LOCUS       NM_001195415            7398 bp    mRNA    linear   PRI 17-NOV-2024
DEFINITION  Homo sapiens doublecortin like kinase 1 (DCLK1), transcript variant
            2, mRNA.
ACCESSION   NM_001195415
VERSION     NM_001195415.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Afshar-Sterle,S., Carli,A.L.E., O'Keefe,R., Tse,J., Fischer,S.,
            Azimpour,A.I., Baloyan,D., Elias,L., Thilakasiri,P., Patel,O.,
            Ferguson,F.M., Eissmann,M.F., Chand,A.L., Gray,N.S., Busuttil,R.,
            Boussioutas,A., Lucet,I.S., Ernst,M. and Buchert,M.
  TITLE     DCLK1 induces a pro-tumorigenic phenotype to drive gastric cancer
            progression
  JOURNAL   Sci Signal 17 (854), eabq4888 (2024)
   PUBMED   39288218
  REMARK    GeneRIF: DCLK1 induces a pro-tumorigenic phenotype to drive gastric
            cancer progression.
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Fukushima,N.M., Adachi,T., Tanaka,T., Matsushima,H., Imamura,H.,
            Hara,T., Soyama,A., Hidaka,M., Kanetaka,K. and Eguchi,S.
  TITLE     DCLK1 Expression and its Functional Significance in Intrahepatic
            Cholangiocarcinoma and Bil-IN Stage
  JOURNAL   Anticancer Res 44 (6), 2417-2424 (2024)
   PUBMED   38821583
  REMARK    GeneRIF: DCLK1 Expression and its Functional Significance in
            Intrahepatic Cholangiocarcinoma and Bil-IN Stage.
REFERENCE   3  (bases 1 to 7398)
  AUTHORS   Ye,L., Liu,B., Huang,J., Zhao,X., Wang,Y., Xu,Y. and Wang,S.
  TITLE     DCLK1 and its oncogenic functions: A promising therapeutic target
            for cancers
  JOURNAL   Life Sci 336, 122294 (2024)
   PUBMED   38007147
  REMARK    GeneRIF: DCLK1 and its oncogenic functions: A promising therapeutic
            target for cancers.
            Review article
REFERENCE   4  (bases 1 to 7398)
  AUTHORS   Chen,X.Y., Kao,C., Peng,S.W., Chang,J.H., Lee,Y.L., Laiman,V.,
            Chung,K.F., Bhavsar,P.K., Heriyanto,D.S., Chuang,K.J. and
            Chuang,H.C.
  TITLE     Role of DCLK1/Hippo pathway in type II alveolar epithelial cells
            differentiation in acute respiratory distress syndrome
  JOURNAL   Mol Med 29 (1), 159 (2023)
   PUBMED   37996782
  REMARK    GeneRIF: Role of DCLK1/Hippo pathway in type II alveolar epithelial
            cells differentiation in acute respiratory distress syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7398)
  AUTHORS   Yasodha,K., Lizha Mary,L., Surajit,P. and Satish,R.
  TITLE     Exosomes from metastatic colon cancer cells drive the proliferation
            and migration of primary colon cancer through increased expression
            of cancer stem cell markers CD133 and DCLK1
  JOURNAL   Tissue Cell 84, 102163 (2023)
   PUBMED   37487255
  REMARK    GeneRIF: Exosomes from metastatic colon cancer cells drive the
            proliferation and migration of primary colon cancer through
            increased expression of cancer stem cell markers CD133 and DCLK1.
REFERENCE   6  (bases 1 to 7398)
  AUTHORS   Lin,P.T., Gleeson,J.G., Corbo,J.C., Flanagan,L. and Walsh,C.A.
  TITLE     DCAMKL1 encodes a protein kinase with homology to doublecortin that
            regulates microtubule polymerization
  JOURNAL   J Neurosci 20 (24), 9152-9161 (2000)
   PUBMED   11124993
REFERENCE   7  (bases 1 to 7398)
  AUTHORS   Matsumoto,N., Pilz,D.T. and Ledbetter,D.H.
  TITLE     Genomic structure, chromosomal mapping, and expression pattern of
            human DCAMKL1 (KIAA0369), a homologue of DCX (XLIS)
  JOURNAL   Genomics 56 (2), 179-183 (1999)
   PUBMED   10051403
REFERENCE   8  (bases 1 to 7398)
  AUTHORS   Sossey-Alaoui,K. and Srivastava,A.K.
  TITLE     DCAMKL1, a brain-specific transmembrane protein on 13q12.3 that is
            similar to doublecortin (DCX)
  JOURNAL   Genomics 56 (1), 121-126 (1999)
   PUBMED   10036192
REFERENCE   9  (bases 1 to 7398)
  AUTHORS   Omori,Y., Suzuki,M., Ozaki,K., Harada,Y., Nakamura,Y., Takahashi,E.
            and Fujiwara,T.
  TITLE     Expression and chromosomal localization of KIAA0369, a putative
            kinase structurally related to Doublecortin
  JOURNAL   J Hum Genet 43 (3), 169-177 (1998)
   PUBMED   9747029
REFERENCE   10 (bases 1 to 7398)
  AUTHORS   Nedivi,E., Hevroni,D., Naot,D., Israeli,D. and Citri,Y.
  TITLE     Numerous candidate plasticity-related genes revealed by
            differential cDNA cloning
  JOURNAL   Nature 363 (6431), 718-722 (1993)
   PUBMED   8515813
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK295777.1, AL157760.9 and
            AL139083.15.
            
            On Aug 13, 2020 this sequence version replaced NM_001195415.1.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. The protein encoded by
            this gene contains two N-terminal doublecortin domains, which bind
            microtubules and regulate microtubule polymerization, a C-terminal
            serine/threonine protein kinase domain, which shows substantial
            homology to Ca2+/calmodulin-dependent protein kinase, and a
            serine/proline-rich domain in between the doublecortin and the
            protein kinase domains, which mediates multiple protein-protein
            interactions. The microtubule-polymerizing activity of the encoded
            protein is independent of its protein kinase activity. The encoded
            protein is involved in several different cellular processes,
            including neuronal migration, retrograde transport, neuronal
            apoptosis and neurogenesis. This gene is up-regulated by
            brain-derived neurotrophic factor and associated with memory and
            general cognitive abilities. Multiple transcript variants generated
            by two alternative promoter usage and alternative splicing have
            been reported, but the full-length nature and biological validity
            of some variants have not been defined. These variants encode
            different isoforms, which are differentially expressed and have
            different kinase activities.[provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (2, also known as DCLK-short-B,
            DCK-beta1 or KIAA0369-BS) is produced from the 3' promoter. It
            lacks several 5' exons but has an alternate 5' exon, as compared to
            variant 1. The resulting isoform (2) has a shorter and different
            N-terminus, and lacks doublecortin domains, as compared to isoform
            1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK295777.1, SRR1803617.123440.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2278              AK295777.1         1-2278
            2279-5209           AL157760.9         2001-4931           c
            5210-7398           AL139083.15        159893-162081       c
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q13.3"
     gene            1..7398
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="doublecortin like kinase 1"
                     /db_xref="GeneID:9201"
                     /db_xref="HGNC:HGNC:2700"
                     /db_xref="MIM:604742"
     exon            1..158
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..1408
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine/threonine-protein kinase DCLK1; doublecortin-like
                     and CAM kinase-like 1; doublecortin domain-containing
                     protein 3A"
                     /codon_start=1
                     /product="serine/threonine-protein kinase DCLK1 isoform 2"
                     /protein_id="NP_001182344.1"
                     /db_xref="CCDS:CCDS73561.1"
                     /db_xref="GeneID:9201"
                     /db_xref="HGNC:HGNC:2700"
                     /db_xref="MIM:604742"
                     /translation="MLELIEVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRSSQHGG
                     SSTSLASTKVCSSMDENDGPGEEVSEEGFQIPATITERYKVGRTIGDGNFAVVKECVE
                     RSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMEL
                     VKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDG
                     SKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCG
                     FPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAVQV
                     LEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIALDHGFTIKRSGSL
                     DYYQQPGMYWIRPPLLIRRGRFSDEDATRM"
     exon            159..253
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            254..338
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            339..447
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            448..505
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            506..625
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            626..772
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            773..906
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            907..984
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1081
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1162
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1276
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1350
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..7398
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4693..4698
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAA"
     polyA_site      4710
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="major polyA site"
     regulatory      7042..7047
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAA"
     regulatory      7047..7052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
                     /note="hexamer: AATAAT"
     polyA_site      7065
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
     polyA_site      7069
                     /gene="DCLK1"
                     /gene_synonym="CL1; CLICK1; DCAMKL1; DCDC3A; DCLK"
ORIGIN      
        1 atctcattca gcctgctctg ggagcagcct tagccatcct taaactcatc caaccgattc
       61 ccatttccgc gggtgcttgg cttagtgctg agacgggatt ccgccgttgg tattcaggtc
      121 caacactaag actgtgtcca tgttagaact catagaagtt aatggaaccc ctggtagtca
      181 gctctctact ccgcgctcag gcaagtcgcc aagcccatca cccaccagcc caggaagcct
      241 gcggaagcag aggagctctc agcatggcgg ctcctctacg tcacttgcgt ccaccaaagt
      301 ctgcagctcg atggatgaga acgatggccc tggagaagaa gtgtcggagg aaggcttcca
      361 gattccagct acaataacag aacgatataa agtcggaaga acaataggag atggaaattt
      421 tgctgttgtc aaggaatgtg tagaaagatc gactgctaga gagtatgctc tgaaaattat
      481 caagaaaagc aaatgtcgag gcaaagagca catgatccag aatgaagtgt ctattttaag
      541 aagagtgaag catcccaata tcgttcttct gattgaggag atggatgtgc caactgaact
      601 gtatcttgtc atggaattag taaagggggg agaccttttt gatgccatta cttccactaa
      661 caaatacacc gagagagacg ccagtgggat gctgtacaac ctagccagcg ccatcaaata
      721 cctgcatagc ctgaacatcg tccaccgtga tatcaagcca gagaacctgc tggtgtatga
      781 gcaccaagat ggcagcaaat cactgaagct gggtgacttt ggactggcca ccattgtaga
      841 cggccccctg tacacagtct gtggcacccc aacatacgtg gctccagaaa tcattgcaga
      901 gactggatac ggcctcaagg tggacatctg ggcagcaggt gtaatcactt atatcctgct
      961 gtgtggtttc cctccattcc gtggaagtgg tgatgaccag gaggtgcttt ttgatcagat
     1021 tttgatgggg caggtggact ttccttctcc atactgggat aatgtttccg attctgcaaa
     1081 ggagctcatt accatgatgc tgttggtcga tgtagatcag cgattttctg ctgttcaagt
     1141 acttgagcat ccctgggtta atgatgatgg cctcccagaa aatgaacatc agctgtcagt
     1201 agctggaaag ataaagaagc atttcaacac aggccccaag ccgaatagca cagcagctgg
     1261 agtttctgtc atagcactgg accacgggtt taccatcaag agatcagggt ctttggacta
     1321 ctaccagcaa ccaggaatgt attggataag accaccgctc ttgataagga gaggcaggtt
     1381 ttccgacgaa gacgcaacca ggatgtgagg agccggtaca aggcgcagcc agctcctccc
     1441 gaactcaact cggaatcgga agactactcc ccaagctcct ccgagactgt tcgctcccct
     1501 aactcgccct tttaataaga cccttttact caaagtccta gcttaaccct ttgagactct
     1561 gagatttttt tcccccaaat ttgtgtaaaa cagtttcatc tgatctatct agcgctcaat
     1621 gcttgaatgg cagaactgaa agtgttttca ggtatctttg tagcggtttc cctttactga
     1681 ataagatgac acgtggtgat tgtgaagatg gtaatttgct gctaatagag tcctcaaagg
     1741 gttaaggcca atttgcaatt tttttttaaa cttagaagca atgaatgttt tcatcagtca
     1801 agctaggatc tgcagtatgt aatatagcac ttgttaaccc tctgagtgca tagaatttta
     1861 ttgagaattc ttgtttggga atttttcagg cctttggatg tatacacaca tgtttcttga
     1921 ttttactgca gatcaagggg tgttgttaga tgctgaaatg tccagaaaag aaggacattt
     1981 agaatgatat cttgtttgtc cttttctgtg ggtttagaac gtggcaggtt tataacttcg
     2041 acacacgcac ggttctttct tcttcacaat cctattcaga aacagatttt ttttttcatt
     2101 agagatatga ctgtcagttg cagtgagttc tgcatcccaa gtggagggaa ttgggtttgt
     2161 ggcaaagagc ttgacccagg aaatagatgg tgccccccaa attgtctcca catgaagatg
     2221 tactgatgac gccccagaaa tgctgcttcc atatcagctg ctgctagcgc cagcgcagac
     2281 tctcagggag tcaccacagc ttgtcttgtg cttggtgagt gagggtctct ctactcagtg
     2341 tcagacatct acaggaaaga aacaactggt ggaaaagagc aataaattgc ccggtgctct
     2401 gcagggctgg aatttcaaac agaaagaggg aataagatcc tgtgattttt ctcacctgct
     2461 tttccacgca ctgtggtcat cactgtgcaa tctacatcta gtatgaaatc cacacatagg
     2521 agagctgggg cacaagggga ctggaggcag ttgctttgca agatggctga ggagaaagca
     2581 cactgggaac acaatccaga atgttctaac aataagtttt cagtgaataa accactggca
     2641 agacaattcc atgtgcacct ttaggttacc tatatagtct cctaggaaga tcaggatgaa
     2701 agacctagat gatacccctg aggataaaac ctccatcccc taaaatgatt ttttttaaat
     2761 accactgtct ttagctgtcc aggaggtcag agtgtttttt ctgtctttgg gccaagtcct
     2821 gtctgagacc tgtattttca ctcttgttac caaatctatc tccctagtgc agtgtctcca
     2881 ggcctgagtt tcttctggaa cagattccat tttagaatgg ggattcacag gttctgtgca
     2941 tcaccacagt gctcagagag gattctcctg gggtgtctta gaggcaggtg cccaactcaa
     3001 atgtattccc aaggtttgct gggctctggg atccacgaga caaccagaga gggatatctc
     3061 atgaaatttg catctggtgg ctgaacagta cctatgttct ctgttttgaa tatactttaa
     3121 tacctgagag tcttaaaatt tgtgaacaac gtttctatag tcctttattt tcaaatgcac
     3181 attgatcttc acttgctgca tttttactct tcaaccctga aactatggtc tacattaata
     3241 tggattttta aatcacatgt cattactttt gcaacaccat caccaaaatt ttttgctctt
     3301 ttacatttag gttcatctct gtggtctgtg ttgtcctgac atgtaaaaag catatcgttt
     3361 attgaggttt ttttcccccc cttttagagc atccggaagt gataacacgc aaaatcacaa
     3421 agtagcataa atcagtaaat tagttgagtt gtttttgggg gggaggtggg ggtagggggc
     3481 acagaacacc agaaagagtg ttggtgtgta ggtagattcc atattaatga ggaacactga
     3541 actagttgga aattactgct ttctctagaa atataaagca aagcactatt ccaaggctat
     3601 ggagtagctc tacagcctgg cctcaactct aaaagtgtga agaatgcaat gggcagagac
     3661 ctacctgcag tggactgtca ttttcctttc tttctctgaa ttactgcttt ttctgtgggc
     3721 attaactata ttgctacagc atctagtgta ctgagcctgc ggtgcatggc tcaggccttt
     3781 tcccatcgac gtctaggggg actctggacc gtgtgaagct agggggtgtt tctcagcaca
     3841 ctgcagaagg gcagctcaga agaatgcagg gcccattcag catggggatc ccagcacatc
     3901 actgtagaat ttgagtgatc tatgctgaat aaacagtgga atgtgaccag tcaagtagaa
     3961 atcttgagta atcagatgga atgcaatctt tctaacatta agctaccaag atcctgaatg
     4021 tcagagatgt actcagaggg ttaacagaca agcacaaggc atgctgacta cattggtgta
     4081 tccagattgc tttgctttta gccagtgctt tctaattttt ttctcgacat tcttgggata
     4141 gttcaagttt gaaataatta agtggtggtg ttctttaagg aatttctata accaaattga
     4201 tcttattttt gatttcactt atcatagaac aaatatgtat cattatggca gtgtatctat
     4261 gtaattatca atttaatcat caccaccggt gtttccatat tttttcccaa gtatttaata
     4321 tagctctctt atggtggtgg cctggtgatg gggaccgtct ttcttttact gacacatgac
     4381 caatcatatg gtattttcaa gggaatttta agattcatct tttcagtttg atagtagact
     4441 agttaaggaa gaactctttc attacttgca tcgtgtaaat catctctgta gacatgtgtt
     4501 catattaatg aacacatttt ttctcaacat tgtagcagaa atcattttat tcgtcatgat
     4561 caatgaatat gtgatttgct ccagatcgtt agaaggaaaa gtaagatttc agtcatcaaa
     4621 aatgttttta ccgtagccct catctaactt acacgtggtg catattaaaa taagcagaga
     4681 aaaaaaaatg tgaataaact actgaaaaca cttggtgttt tgtgttcaat gagaccttcc
     4741 tgcaacctgc tccccatggg tggcagttaa caggcccatc agatattgtt gaaagaaagc
     4801 aatatatcca tgaatgaagg ctaaaattgc aatcctttac cctttgaggc atatttcagt
     4861 tgaaaacaaa aagaaaagaa aatttggctt agagggtcac agagctccca tatgaccaag
     4921 tctcaagcac attaaatcat ggttgtttac tggccaaggg cgtccactag acaactctat
     4981 cccttgcgct gaagctcaat cgtgctgagg gagagctttc ttaatattac tgtgttgctc
     5041 ttagcccttc tctgggttag gatctgtcag catttctatg ataaactcct attctcaaag
     5101 gtttttaatt tgaccataaa aatgtgcccc aggctgaagt ttgctataca gggctgtacc
     5161 aaagagtgaa ggtttacttc cttctctttc caacttcttc cccattctcc aaggaaaaga
     5221 acaacaaaaa aatctggtat ggtccctcct taatagtgat ttcagaattt tggaaagcac
     5281 caagatccaa gatggtagtt ttaatgtagt tactcattcg cacacatttt ttaaatttaa
     5341 tgggtcacct ggcatatatt gtagataaca tatcttttct ataatttgta agtcaataat
     5401 ttttttaact gctacatgat attttttttt gcccaaagat tttaaaagac ttgaagttgg
     5461 tcagttcaaa actcagattt ttctacacat tgtctgccat gtccattagg agtttgggga
     5521 aaatactctc acacagaccc ttactttgca tgcagtttag agggtaagat acgtgcttct
     5581 tttggggata aagatttcct tacttaattg tcaaatttca tggagccatt ctagtctgtt
     5641 ggggaaaata gtgattaaaa gcacttccaa aattaacatt ttttgacaat tcagatatga
     5701 aaagaagcag gggaaaataa tacactttac tcttttcttg cttaaaggca aacaaatcaa
     5761 tgaaacttga ggacacacta aacatttgat aactgcaaat gtgctttaaa aattggttca
     5821 atggtgctta cacatgaaac ggtaacaaat ggggttccta ggacgtcaga aggaatcttt
     5881 agtttgtatg taattacaca ctagaggagg aggtgctttt aagccagtct tttattttta
     5941 atcatctcaa atatgcaacc atacatgcag taacattaag ggtcgtaaac tggtgggaaa
     6001 caggaacttc agtggagagg cttaaatgcc tctggttaga gtgggggttt ttgtttgttt
     6061 gtttattgtt gggtttcaac actgagcatc atttctgtga tcaagtttct aactggcatg
     6121 tgttttgatc atgaggttta ccatatcttg cccatacaga caaatgagag atctagtttc
     6181 attttgttcc ctaaagaaag aacactctct aaaattaaat catacctgta aatttcttca
     6241 gcatttgttt ctgttcaatg aaattgagac ccttaatgtt gctttaatgt aaaattgaat
     6301 attttgtctg tgatatactt taataattta aagtaagtaa tagttctaaa gtcttcactg
     6361 ttgctactaa gagaaaatag aattttaaag gtgatgataa agatgctata atgtcagttc
     6421 actccagtcc aatcaaatgt agtaagaaaa agtccttgaa tagttctcta gggacaattt
     6481 ctcacttgcc attgacatta atctttggtg tattctcaga aaaaataaaa agaaattgaa
     6541 actggtccaa ggttatagtc atatcctcga taacttttga aaaaaaattt tattaggaaa
     6601 ttaatactag cctttttcat tctggctgaa agaaaattat taaaggatta gttgagtgtg
     6661 aaattcaaca gtattttgct catacatact aaaaaggtgc gtagggactt ggcgcattta
     6721 aacaagtttc tgaaaggttt caatttgact caagaaaaaa attcaatatt tcttttgaaa
     6781 atactgaatt tatcacttgc tgcatggatc agatggcata ggttaatctt tgattttcag
     6841 aatcctaatg aaataacttt caaacaattt gtgtccttaa ttaaaggtgg aatgagatcc
     6901 aatttttccc cctaatcctt cagtttaagc tgatacatgt aggttaatgt ggaatgaaat
     6961 catctgtgat atattatgtt catttatcaa ctgagctttt ttgatgttgc ctgtttttat
     7021 gtaaaacatg ttcttaaagt taataaaata atagtacttg gtgtatgaac tactacataa
     7081 taccttagct gaagggtacg ctcctcagtt catttgccat attggagtaa aatttcacct
     7141 tagttcaatt cacaattgac acattcagtt cacgaaggaa tagcccagct gtgttcttag
     7201 ggggaattct ctctatgcaa ggctttaaaa attagtcatg cggtcagagt gtagctttcc
     7261 cacatgtcct ccatagcaca gtgtgtattt catgagcata attcaaaaac agctatttca
     7321 aaaatgtccc ttgtcatata ttacgttcct gcctgggcca ctgacttgga taaatacaaa
     7381 taaaccaaat accattca
//
