LOCUS       NM_000788               2506 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens deoxycytidine kinase (DCK), mRNA.
ACCESSION   NM_000788
VERSION     NM_000788.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Dash,S., Ueda,T., Komuro,A., Honda,M., Sugisawa,R. and Okada,H.
  TITLE     Deoxycytidine kinase inactivation enhances gemcitabine resistance
            and sensitizes mitochondrial metabolism interference in pancreatic
            cancer
  JOURNAL   Cell Death Dis 15 (2), 131 (2024)
   PUBMED   38346958
  REMARK    GeneRIF: Deoxycytidine kinase inactivation enhances gemcitabine
            resistance and sensitizes mitochondrial metabolism interference in
            pancreatic cancer.
            Erratum:[Cell Death Dis. 2024 May 31;15(5):384. doi:
            10.1038/s41419-024-06628-3.. PMID: 38821944]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Yoshida,K., Fujita,A., Narazaki,H., Asano,T. and Itoh,Y.
  TITLE     Drug resistance to nelarabine in leukemia cell lines might be
            caused by reduced expression of deoxycytidine kinase through
            epigenetic mechanisms
  JOURNAL   Cancer Chemother Pharmacol 89 (1), 83-91 (2022)
   PUBMED   34825941
  REMARK    GeneRIF: Drug resistance to nelarabine in leukemia cell lines might
            be caused by reduced expression of deoxycytidine kinase through
            epigenetic mechanisms.
REFERENCE   3  (bases 1 to 2506)
  AUTHORS   Hosokawa,M., Seiki,R., Iwakawa,S. and Ogawara,K.I.
  TITLE     Combination of azacytidine and curcumin is a potential alternative
            in decitabine-resistant colorectal cancer cells with attenuated
            deoxycytidine kinase
  JOURNAL   Biochem Biophys Res Commun 578, 157-162 (2021)
   PUBMED   34571370
  REMARK    GeneRIF: Combination of azacytidine and curcumin is a potential
            alternative in decitabine-resistant colorectal cancer cells with
            attenuated deoxycytidine kinase.
REFERENCE   4  (bases 1 to 2506)
  AUTHORS   Hellendahl,K.F., Fehlau,M., Hans,S., Neubauer,P. and Kurreck,A.
  TITLE     Semi-Automated High-Throughput Substrate Screening Assay for
            Nucleoside Kinases
  JOURNAL   Int J Mol Sci 22 (21), 11558 (2021)
   PUBMED   34768989
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2506)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2506)
  AUTHORS   Eriksson,S., Cederlund,E., Bergman,T., Jornvall,H. and Bohman,C.
  TITLE     Characterization of human deoxycytidine kinase. Correlation with
            cDNA sequences
  JOURNAL   FEBS Lett 280 (2), 363-366 (1991)
   PUBMED   2013338
REFERENCE   7  (bases 1 to 2506)
  AUTHORS   Chottiner,E.G., Shewach,D.S., Datta,N.S., Ashcraft,E., Gribbin,D.,
            Ginsburg,D., Fox,I.H. and Mitchell,B.S.
  TITLE     Cloning and expression of human deoxycytidine kinase cDNA
  JOURNAL   Proc Natl Acad Sci U S A 88 (4), 1531-1535 (1991)
   PUBMED   1996353
REFERENCE   8  (bases 1 to 2506)
  AUTHORS   Datta,N.S., Shewach,D.S., Mitchell,B.S. and Fox,I.H.
  TITLE     Kinetic properties and inhibition of human T lymphoblast
            deoxycytidine kinase
  JOURNAL   J Biol Chem 264 (16), 9359-9364 (1989)
   PUBMED   2542307
REFERENCE   9  (bases 1 to 2506)
  AUTHORS   Datta,N.S., Shewach,D.S., Hurley,M.C., Mitchell,B.S. and Fox,I.H.
  TITLE     Human T-lymphoblast deoxycytidine kinase: purification and
            properties
  JOURNAL   Biochemistry 28 (1), 114-123 (1989)
   PUBMED   2539852
REFERENCE   10 (bases 1 to 2506)
  AUTHORS   Bohman,C. and Eriksson,S.
  TITLE     Deoxycytidine kinase from human leukemic spleen: preparation and
            characteristics of homogeneous enzyme
  JOURNAL   Biochemistry 27 (12), 4258-4265 (1988)
   PUBMED   2844225
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI601939.1, CD014016.1 and
            BC103764.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000788.2.
            
            Summary:  Deoxycytidine kinase (DCK) is required for the
            phosphorylation of several deoxyribonucleosides and their
            nucleoside analogs.  Deficiency of DCK is associated with
            resistance to antiviral and anticancer chemotherapeutic agents.
            Conversely, increased deoxycytidine kinase activity is associated
            with increased activation of these compounds to cytotoxic
            nucleoside triphosphate derivatives.  DCK is clinically important
            because of its relationship to drug resistance and sensitivity.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC103764.1, SRR1660803.117849.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000286648.10/ ENSP00000286648.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-425               BI601939.1         103-527
            426-1312            CD014016.1         528-1414
            1313-2163           BC103764.1         1325-2175
            2164-2506           CD014016.1         2266-2608
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q13.3"
     gene            1..2506
                     /gene="DCK"
                     /note="deoxycytidine kinase"
                     /db_xref="GeneID:1633"
                     /db_xref="HGNC:HGNC:2704"
                     /db_xref="MIM:125450"
     exon            1..278
                     /gene="DCK"
                     /inference="alignment:Splign:2.1.0"
     CDS             188..970
                     /gene="DCK"
                     /EC_number="2.7.1.74"
                     /EC_number="2.7.1.113"
                     /EC_number="2.7.1.76"
                     /note="deoxynucleoside kinase; deoxyadenosine kinase;
                     deoxyguanosine kinase"
                     /codon_start=1
                     /product="deoxycytidine kinase"
                     /protein_id="NP_000779.1"
                     /db_xref="CCDS:CCDS3548.1"
                     /db_xref="GeneID:1633"
                     /db_xref="HGNC:HGNC:2704"
                     /db_xref="MIM:125450"
                     /translation="MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQL
                     CEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACL
                     SRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHD
                     WMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLH
                     RTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL"
     exon            279..394
                     /gene="DCK"
                     /inference="alignment:Splign:2.1.0"
     exon            395..588
                     /gene="DCK"
                     /inference="alignment:Splign:2.1.0"
     exon            589..736
                     /gene="DCK"
                     /inference="alignment:Splign:2.1.0"
     exon            737..852
                     /gene="DCK"
                     /inference="alignment:Splign:2.1.0"
     exon            853..943
                     /gene="DCK"
                     /inference="alignment:Splign:2.1.0"
     exon            944..2506
                     /gene="DCK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2471..2476
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCK"
                     /note="hexamer: AATAAA"
     polyA_site      2506
                     /gene="DCK"
                     /note="major polyA site"
ORIGIN      
        1 agtgtcctca gctgcctccg cgcgccaaag tcaaaccccg acacccgccg gcgggccggt
       61 gagctcacta gctgacccgg caggtcagga tctggcttag cggcgccgcg agctccagtg
      121 cgcgcacccg tggccgcctc ccagccctct ttgccggacg agctctgggc cgccacaaga
      181 ctaaggaatg gccaccccgc ccaagagaag ctgcccgtct ttctcagcca gctctgaggg
      241 gacccgcatc aagaaaatct ccatcgaagg gaacatcgct gcagggaagt caacatttgt
      301 gaatatcctt aaacaattgt gtgaagattg ggaagtggtt cctgaacctg ttgccagatg
      361 gtgcaatgtt caaagtactc aagatgaatt tgaggaactt acaatgtctc agaaaaatgg
      421 tgggaatgtt cttcagatga tgtatgagaa acctgaacga tggtctttta ccttccaaac
      481 atatgcctgt ctcagtcgaa taagagctca gcttgcctct ctgaatggca agctcaaaga
      541 tgcagagaaa cctgtattat tttttgaacg atctgtgtat agtgacaggt atatttttgc
      601 atctaatttg tatgaatctg aatgcatgaa tgagacagag tggacaattt atcaagactg
      661 gcatgactgg atgaataacc aatttggcca aagccttgaa ttggatggaa tcatttatct
      721 tcaagccact ccagagacat gcttacatag aatatattta cggggaagaa atgaagagca
      781 aggcattcct cttgaatatt tagagaagct tcattataaa catgaaagct ggctcctgca
      841 taggacactg aaaaccaact tcgattatct tcaagaggtg cctatcttaa cactggatgt
      901 taatgaagac tttaaagaca aatatgaaag tctggttgaa aaggtcaaag agtttttgag
      961 tactttgtga tcttgctgaa gactacaggc agccaaatgg ttccagatac ttcagctttg
     1021 tgtatcttcg taacttcata ttaatataag tttctttaga aaacccaagt ttttaatcgt
     1081 ttttgtttta aggaaaaaag atttttaaaa tgaatcttat gcaaaacttt ttgaccagtt
     1141 tcttttcttt tgtttttttt ttaaaaaaga catttaaaga caaagacatt atttctcata
     1201 gcaggaaatg tagaggtaga tggttccagt atcagcatag tgactaaact acattataaa
     1261 agatccagct tccttctgtc attcccctct tttgtcttcc tcagcaggtt ggcttttttc
     1321 cctggtgcct ctcacttcgt tggtgaccag tttcttaaac tgaaagcttt aatgttacat
     1381 agtaaatggt agtgtgtcct gtgtaaatta gtgtacctat taaaagttgc aaagtggaat
     1441 taaaggaatc cctagaataa ggattctgaa gttttatttt aaattattat cttcttaaca
     1501 gtttagtccc acctcttact tcctgcctca gtctgctttc tctactgtct ggattaatta
     1561 ggcagcctgc tataaagtta aagtcacaca tttctatttt gcaaacactg tgattactct
     1621 ttgctttgta gtttgctttg ctttgtaggg ttctgctttt aagtttttct ctttttcaga
     1681 caaattactg ataaaaatga tattgctcta tatgtaatat atcctgaaag cattattttt
     1741 tgttgaatag gaaataaaat taatgaagac agaggctaga aagcatccat taattaatga
     1801 gacacactta actacttatc tctaaaccat ctatgtgaat atttgtaaaa ataatgaatg
     1861 gactcatctt agttctgtat ataaatatat tttctttcta gtttgtttag ttaaggtgtg
     1921 cagtgttttt cctgtgtatt aaacctttcc attttacgtt ttagaaaatt ttatgtattt
     1981 taaaataagg ggaagagtca ttttcacttt taaactacta tttttctttc caagtcattt
     2041 ttgtttttgg tttcttattc aaagatgata atttagtgga ttaaccagtc cagacgcact
     2101 gatctttgca aaggagactt aatttcaaat ctgtaattac catacataaa ctgtctcatt
     2161 atacgtatgc atttttttag tttgtttttg tttggtataa attaatttgt taattaaata
     2221 tttcttaagt ataaacctta tgaactacag tggagctaca ctcattgaaa tgtaatttca
     2281 gttctaaaaa gatgtaataa tcattttaga attaaaattt attctacttt taaataaatt
     2341 atgaatatta aaggtgaaaa ttgtataaat tactttgatt ccattttaag tggagacata
     2401 tttcagtgat ttttagtaac ctttaaaaat gtataatgac ttttaaaatt tgtagaattg
     2461 aaaagacgct aataaaaatt tattatttat ttgtcatgac tcaaaa
//
