LOCUS       NM_001358235           13354 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens dachsous cadherin-related 2 (DCHS2), transcript
            variant 3, mRNA.
ACCESSION   NM_001358235
VERSION     NM_001358235.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Lodge,E.J., Xekouki,P., Silva,T.S., Kochi,C., Longui,C.A.,
            Faucz,F.R., Santambrogio,A., Mills,J.L., Pankratz,N., Lane,J.,
            Sosnowska,D., Hodgson,T., Patist,A.L., Francis-West,P.,
            Helmbacher,F., Stratakis,C. and Andoniadou,C.L.
  TITLE     Requirement of FAT and DCHS protocadherins during
            hypothalamic-pituitary development
  JOURNAL   JCI Insight 5 (23), 134310 (2020)
   PUBMED   33108146
  REMARK    GeneRIF: Requirement of FAT and DCHS protocadherins during
            hypothalamic-pituitary development.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13354)
  AUTHORS   Vieira,R.N., Avila,R., de Paula,J.J., Cintra,M.T., de Souza,R.P.,
            Nicolato,R., Malloy-Diniz,L., de Miranda,D.M., de Moraes,E.N., de
            Marco,L.A., Romano-Silva,M.A. and Bicalho,M.A.
  TITLE     Association between DCHS2 gene and mild cognitive impairment and
            Alzheimer's disease in an elderly Brazilian sample
  JOURNAL   Int J Geriatr Psychiatry 31 (12), 1337-1344 (2016)
   PUBMED   26876984
  REMARK    GeneRIF: We found significant differences between the genotype
            frequency of DCHS2 between controls and amnestic mild cognitive
            impairment but not between controls and Alzheimers disease.
REFERENCE   3  (bases 1 to 13354)
  AUTHORS   Adhikari,K., Fuentes-Guajardo,M., Quinto-Sanchez,M.,
            Mendoza-Revilla,J., Camilo Chacon-Duque,J., Acuna-Alonzo,V.,
            Jaramillo,C., Arias,W., Lozano,R.B., Perez,G.M., Gomez-Valdes,J.,
            Villamil-Ramirez,H., Hunemeier,T., Ramallo,V., Silva de
            Cerqueira,C.C., Hurtado,M., Villegas,V., Granja,V., Gallo,C.,
            Poletti,G., Schuler-Faccini,L., Salzano,F.M., Bortolini,M.C.,
            Canizales-Quinteros,S., Cheeseman,M., Rosique,J., Bedoya,G.,
            Rothhammer,F., Headon,D., Gonzalez-Jose,R., Balding,D. and
            Ruiz-Linares,A.
  TITLE     A genome-wide association scan implicates DCHS2, RUNX2, GLI3, PAX1
            and EDAR in human facial variation
  JOURNAL   Nat Commun 7, 11616 (2016)
   PUBMED   27193062
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 13354)
  AUTHORS   An,C.H., Je,E.M., Yoo,N.J. and Lee,S.H.
  TITLE     Frameshift mutations of cadherin genes DCHS2, CDH10 and CDH24 genes
            in gastric and colorectal cancers with high microsatellite
            instability
  JOURNAL   Pathol Oncol Res 21 (1), 181-185 (2015)
   PUBMED   24898286
REFERENCE   5  (bases 1 to 13354)
  AUTHORS   Miyashita,A., Hatsuta,H., Kikuchi,M., Nakaya,A., Saito,Y.,
            Tsukie,T., Hara,N., Ogishima,S., Kitamura,N., Akazawa,K.,
            Kakita,A., Takahashi,H., Murayama,S., Ihara,Y., Ikeuchi,T. and
            Kuwano,R.
  CONSRTM   Japanese Alzheimer's Disease Neuroimaging Initiative
  TITLE     Genes associated with the progression of neurofibrillary tangles in
            Alzheimer's disease
  JOURNAL   Transl Psychiatry 4 (6), e396 (2014)
   PUBMED   26126179
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13354)
  AUTHORS   Han,Y., Pei,Y., Liu,Y., Zhang,L., Wu,S., Tian,Q., Chen,X., Shen,H.,
            Zhu,X., Papasian,C.J. and Deng,H.
  TITLE     Bivariate genome-wide association study suggests fatty acid
            desaturase genes and cadherin DCHS2 for variation of both
            compressive strength index and appendicular lean mass in males
  JOURNAL   Bone 51 (6), 1000-1007 (2012)
   PUBMED   22960237
  REMARK    GeneRIF: Findings suggest that the 3 genes, FADS1, FADS2, and
            DCHS2, containing these SNPs might play dual roles influencing both
            CSI and ALM in males.
REFERENCE   7  (bases 1 to 13354)
  AUTHORS   Kamboh,M.I., Barmada,M.M., Demirci,F.Y., Minster,R.L.,
            Carrasquillo,M.M., Pankratz,V.S., Younkin,S.G., Saykin,A.J.,
            Sweet,R.A., Feingold,E., DeKosky,S.T. and Lopez,O.L.
  CONSRTM   Alzheimer's Disease Neuroimaging Initiative
  TITLE     Genome-wide association analysis of age-at-onset in Alzheimer's
            disease
  JOURNAL   Mol Psychiatry 17 (12), 1340-1346 (2012)
   PUBMED   22005931
  REMARK    GeneRIF: DCHS2 gene is expressed in the cerebral cortex and thus is
            a potential candidate for affecting Age of onset in AD.
REFERENCE   8  (bases 1 to 13354)
  AUTHORS   Hoeng,J.C., Ivanov,N.V., Hodor,P., Xia,M., Wei,N., Blevins,R.,
            Gerhold,D., Borodovsky,M. and Liu,Y.
  TITLE     Identification of new human cadherin genes using a combination of
            protein motif search and gene finding methods
  JOURNAL   J Mol Biol 337 (2), 307-317 (2004)
   PUBMED   15003449
  REMARK    Erratum:[J Mol Biol. 2014 Feb 6;426(3):775. Hong, Julia C
            [corrected to Hoeng, Julia C]]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC110775.3, BC140919.1,
            AY354498.1, AK127704.1, AY354497.1, BX647835.1 and AC079298.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001358235.1.
            
            Summary: This gene encodes a large protein that contains many
            cadherin domains and likely functions in cell adhesion. Genome-wide
            association studies suggest that this gene may be important in
            Alzheimer's disease, compressive strength index, and appendicular
            lean mass. [provided by RefSeq, May 2017].
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357232.10/ ENSP00000349768.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-396               AC110775.3         206658-207053       c
            397-465             BC140919.1         1-69
            466-2302            AY354498.1         1-1837
            2303-2688           AK127704.1         2221-2606
            2689-2920           AY354497.1         463-694
            2921-4174           AY354497.1         980-2233
            4175-10560          AY354497.1         2366-8751
            10561-10721         BX647835.1         3728-3888
            10722-13354         AC079298.8         152278-154910       c
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q31.3"
     gene            1..13354
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /note="dachsous cadherin-related 2"
                     /db_xref="GeneID:54798"
                     /db_xref="HGNC:HGNC:23111"
                     /db_xref="MIM:612486"
     exon            1..2496
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     CDS             445..10560
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /note="isoform 3 is encoded by transcript variant 3;
                     cadherin-like 27; protocadherin 23; cadherin-related
                     family member 7; cadherin-like protein VR8; protocadherin
                     PCDHJ; cadherin-like protein CDHJ; protein dachsous
                     homolog 2; protocadherin J; cadherin J"
                     /codon_start=1
                     /product="protocadherin-23 isoform 3"
                     /protein_id="NP_001345164.1"
                     /db_xref="CCDS:CCDS87275.1"
                     /db_xref="GeneID:54798"
                     /db_xref="HGNC:HGNC:23111"
                     /db_xref="MIM:612486"
                     /translation="MSPCGRKMGEGRQQRRAPVGKLLLLPGRRDTPHGRSGSSGARTQ
                     RSLLWLLVHVWLWAASGSSAQLFNLTLSVDEGLPPDTLVGDIRAGLPAAQQQEGSGFF
                     LSEDSDDSPLLDDFHVHPDTGIIRTARRLDRERRDHYSFVAATLLGAVVQVEIRVNDV
                     NDHSPRFPLDSLQLDVSELSPPGTAFRLPVAHDPDAGLFSTQGYTLVQPSDLPKDPAG
                     PFFQLRYRTPGPLPSPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRR
                     TGLLSVELRVLDENDNPPVFEQDEYRAAVREDAQPGAEVCRVRATDRDLGPNGFVRYS
                     VRARQVPGAGSGGGALGDAAYFAVEELSGVVRVWRPLDREAQAWHQLVVEARDGGAEP
                     EVATVRVSIAVLDVNDNRPAIHVLFLTEGGVARVSEGARPGDYVARVSVSDADGDWEK
                     EDEATGELGVGLGDGSISLSLEGGEGDFALLPGGPPGVFFLCVEGPLDRESRDLYELL
                     LVATDAGSPPLSTEETLLLRVADLNDQPPLFSQQHYKASVSEAAAPGTVVMWVSASDA
                     DEAGSDHAWLRYTVVQLSAPCNLGSLQSKMVHTAECGPSFAIDSESGAISTIRTLDRE
                     VQEAVELKVVAQDLGEPPLSATCLVSITVDDVNDNEPIFWRQVYNATIAEHAPVGHCF
                     LQVTASDADSGLYGFIEYSLYDGFLSYEAPQAFRIDPHDGQICVSQDIDRERDPATYD
                     LLVEAKDGGGLSAQAFVRVDLEDVNDNHPVFNPSTYVTSISDETQPGTEIINVLATDQ
                     DSGIYGTVAYELIPGNVSSLFTIDSTTGIIYLTLPLSHLESTTLSLMVSAQDGGGLTA
                     VINADVTIHIFQTTLAPAEFERPKYTFLVYEDVPEDSPIGTVKAREPLNSSEPIFYRI
                     SSGDLGGKFSIHPRLGTIRTRKPLDHETQPVVVLTVQAQLGSAPACSSTEVNITVMDV
                     NDNHPAFLRTSDEIRISQTTPPGTALYLARAEDRDSGRNGLIRYSIASPQPGVFAIDR
                     ALGVLFLNGSLGAGEQRELTLTLRAEDQGVHPQAALLVLTVVIEKREHSPSWTFEHLV
                     YQVEVSESLSPMTQMLQTQAHPLGPQRAASPLRYSLEPSVDSAMFGIRPYTGWIYLRR
                     QFDYESTQTYNFRVFAWIPEDGFLQNVSTTVIVRVWDENDNSPTFLHDVLFLKVEESP
                     VPQGVIGKITAIDMDSGKNGQLLYFLLSDGKFFKMNPNTGELINWVALDREHRGHHEM
                     TVLVTDRGSPPRNATMAVYVSVTDINDNRPFFPQCLPGKELHVKVLEGQPVNMLVTTV
                     FAKDPDEGNNAEVTYSVSSEDSSDHFKIDANNGEIRTTTILSYDYRPSYRMSVIATDQ
                     GVPPLQGQAVVNIQVIPLSKGRAIMSQNIRHLIIPENLKPTKIMSLIKSSDHLQQHYN
                     GKLHFSIVADDKDGHFEIDSSTGDLFLSKELDYETTSHYLFRVITTDHSKNLSLSSTV
                     FLSIDVEDQNDHSPSFQDELIVISVEENVPIGTLVYVFNAKDDDGSFLNSRIQYYIES
                     HNPGTNPFLIHPSFGTLVTVSRLDRESIPTVILTVTASDQAVNVTDRRLRSLTAQIVI
                     LDVNDHNPTFISFPNAHVKEDVTVGSLVHHITAHDPDEGRNGKVTYSILSGNENMTFM
                     LDESSGLLTTTCPLDYEMKTQHILTVLALDDGTPALSSSQTLTVTVLDVNDEAPVFKQ
                     HLYEASVKENQNPGEFVTRVEALDRDSGVNSKLQFEIMPGASFELFEINSDTGEVVTT
                     TILDREIQEVFTLRVLVRDGGFPSLSSTTTILCTVEDENDHAPEFIVSSYDIEVLENQ
                     EPEVVYTVLASDMDAGNNRAVEYHIIDGNTDECFTINEMSGELSTTRALDREQISNFT
                     LVILCSDLGDPPRSSVIHLQVRVLDANDHSPSFPTLYYQSSVREDAEVGTVVLVLSAV
                     DKDEGLNGQTEYFLTDEASGAFTIDPMSGTLKTSNTLDREARSQHTFSAVARDCSIQG
                     SRSTTVIIKVYVTDVNDNDPVLEQNPFDVFLSPESPTNQTTVIVRADDLDLGPNGTVV
                     FSFAETQSMFSIDKYTGEIQFQQNPSSEYFPIWLQLKVTDQGIPARTTTGLLVIHMEG
                     EDVKISFSHHLYKGLVTENCEAGTSIVTVKAFAPDSIQDSMKYSIFSGNEDGVLSLCS
                     KSGQLTVKEPKFLDFEVRNEVQLIVLAESSGHRAYCKVAVLIQDENDNSPCFEQSIYQ
                     ASVSESQLYNAHVIQVFATDLDSGLNGLIEYSILSGNQEEAFQIDALSGVITTKAILD
                     YELTSSYSLIVQATDKGMPRLSNTTVIKVQVTDINDNAPAFLPSEAVEITEDSLPGVI
                     VTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQI
                     SDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENI
                     SYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIG
                     IEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQN
                     NFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQ
                     VLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGH
                     FYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFM
                     FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLID
                     PLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYF
                     FTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLI
                     KSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSF
                     LVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSN
                     EVVPVDATPEWLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKC
                     SDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTT
                     CAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHW
                     NYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAV
                     PPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGE
                     LLGTHISGTCHELKAEDEVQI"
     exon            2497..2688
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2920
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..3157
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3158..4174
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4175..4362
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4363..4462
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4463..4620
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4621..5464
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            5465..5704
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            5705..5839
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            5840..6049
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            6050..6907
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            6908..7021
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            7022..7233
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            7234..7385
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            7386..7516
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            7517..7803
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            7804..7936
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     exon            7937..13354
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10702..10707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /note="hexamer: ATTAAA"
     polyA_site      10726
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
     regulatory      12824..12829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /note="hexamer: AATAAA"
     polyA_site      12849
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /note="major polyA site"
     regulatory      13257..13262
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
                     /note="hexamer: ATTAAA"
     polyA_site      13277
                     /gene="DCHS2"
                     /gene_synonym="CDH27; CDHJ; CDHR7; PCDH23; PCDHJ"
ORIGIN      
        1 actccacccc ctactccggc cagatcccca tcctctcctt gtgtgtgcgc gcttcatata
       61 atctcctcct ttttggcaaa agaaataatg cacctgactt taccaggggg gaacaaccag
      121 ccgagtagaa caaggaacag atgtaaaggg aataaaagga gagagaaaaa gagatgagat
      181 tcgtttaaag aaatccaggg gcagaagagg tggctgccgc ggcagaggca gctagagctt
      241 acttccctgt ctgcgtgagc tgcaggcaga ggacgctttc accagttgca gatgtaacct
      301 cgggaattcc tgggccgtcg gtttgtttgc caaacaaagt cttttcttct ctggctcaga
      361 cactgaagag gctcctggat ttttctgcga tcctgccaca agctggcagt tacccgagag
      421 ccttcccaag gagctggagg agaaatgagc ccttgtgggc ggaagatggg cgaagggcgt
      481 cagcagcggc gggctccggt cgggaagctc cttctgctcc ccgggaggag agatacaccc
      541 catgggcggt caggcagcag cggcgccagg acgcagcgct ccctgctctg gctcttggtg
      601 cacgtgtggc tgtgggcggc ctcgggctcc tctgcccagt tgttcaacct caccctttcc
      661 gtagatgagg ggcttccccc ggacacgctg gtaggtgaca tccgcgccgg gctgccggcc
      721 gcgcagcagc aggaggggag cggcttcttt ctgtcggagg actccgatga ctccccgctg
      781 ctggacgact tccacgtgca cccggacacc ggcatcatcc gcactgcgcg gcgcctggac
      841 cgcgagcggc gggaccacta cagcttcgtc gccgccacgc tgctgggcgc tgtggtgcag
      901 gtggagattc gcgtcaacga cgtgaatgac cactcgcccc gctttcccct cgactccctg
      961 caactcgacg tctccgagct cagcccgcca gggaccgcct tccgcctgcc agttgcccac
     1021 gatccggacg ccggactgtt cagcactcag ggctacaccc tggtgcaacc gtccgacctg
     1081 cccaaggacc ccgcaggccc gttcttccag ttgcgctacc ggactccggg gccactaccg
     1141 tcaccgcttt tgccaggctc ctcgtcaccc ctggagcctc tagatctggt gctgctgcgg
     1201 cgcttggacc gagaggaggc ggcggcgcac cggctgcaga tcgaggcatg ggacggcggc
     1261 cgaccccggc gcaccggcct cctgagcgtg gagctgcgcg tgctggatga gaacgacaac
     1321 ccgccggtct ttgagcagga cgagtaccgc gccgcggtgc gcgaggacgc ccagccgggc
     1381 gccgaggtct gtcgcgtgcg cgccaccgac cgcgacctgg ggcccaatgg cttcgtgcgc
     1441 tacagcgtcc gcgcccggca agtgcctggg gcgggtagcg gcggcggggc actgggcgac
     1501 gcggcctact tcgcggtgga ggagctgagc ggcgtggtgc gagtgtggag acctctggac
     1561 cgcgaggcac aggcctggca ccagttggtg gtggaggccc gcgatggagg cgccgagcct
     1621 gaggttgcca cggtgcgcgt gtccatcgcc gtgctggacg tgaatgacaa ccggccagca
     1681 attcacgtgc tctttctcac agagggaggc gtcgcccgtg tctctgaagg cgcccgaccg
     1741 ggcgactacg tggctcgcgt ctcggtgtct gacgcggacg gtgactggga gaaggaagat
     1801 gaggccacag gggagcttgg tgtgggtctt ggagacggga gcatctctct gtccttggaa
     1861 ggcggagagg gagacttcgc gttgctaccc ggcggccccc caggggtatt tttcctttgc
     1921 gtggaggggc ccctggacag agagagccgc gatctgtatg agttactact ggtggccacg
     1981 gacgcggggt ccccgccgct gagcacggag gagacgctgc tactccgggt cgctgacctc
     2041 aatgaccaac cacctctctt cagccaacag cattacaagg cctcagtgtc cgaggccgcg
     2101 gcccctggca ctgtagtcat gtgggtcagc gcctccgatg ccgacgaggc aggcagtgat
     2161 cacgcctggc tgcgctacac tgtagtccaa ctctcggctc cctgcaatct cggctccctg
     2221 caatcaaaga tggtccacac cgcagagtgt ggaccatctt ttgccattga ttccgaaagc
     2281 ggtgcgatca gcactatccg gactctagac cgagaggtcc aggaggcggt ggagctgaaa
     2341 gtggtggccc aggacctcgg agagccccca ctctctgcca cctgcctggt gagcatcacc
     2401 gtagatgatg tgaatgacaa tgagcccatc ttctggaggc aggtgtacaa tgccaccatt
     2461 gcagagcatg ccccggttgg acactgcttt ctgcaggtga cagcctctga tgcagattca
     2521 ggactctatg gctttattga atattctctt tatgatggat tcctgagcta tgaagcacct
     2581 caggcattcc ggatcgaccc tcatgatggg caaatctgtg tttctcaaga tatcgacagg
     2641 gaaagggatc cagctaccta tgatctcctg gtggaagcta aggatggggg tgggctaagt
     2701 gcccaagcct ttgttcgtgt ggacctggag gacgtgaatg ataatcatcc tgtgtttaac
     2761 ccatcaacct atgtgacgag catcagtgat gagacccagc caggcaccga gatcatcaat
     2821 gttcttgcca ctgaccagga ctctgggata tatgggacag tggcttatga gcttattcca
     2881 ggaaacgtgt cgtccctttt taccattgac tccaccacag gaattattta cttaacatta
     2941 cctcttagtc atttggaatc taccacactt tcgttgatgg tctctgctca agacggtggt
     3001 gggctcacag ctgtcattaa tgccgatgtc accatacaca ttttccagac aactctggca
     3061 cctgctgagt ttgaaaggcc taagtacact ttcttagttt atgaagatgt gcctgaagat
     3121 agtcccattg gaacagtgaa agcaagagag cccttgaact cctcagaacc aatcttttac
     3181 aggatttctt ctggtgatct cggcggaaag ttctccattc acccgcggct gggcactatt
     3241 cgcacccgga agcccctgga tcacgagacg cagcccgtgg ttgtgctcac ggtgcaggcg
     3301 cagctcggca gcgccccagc ctgcagcagc accgaggtca acataacagt catggatgtc
     3361 aatgacaacc acccagcgtt cctcaggacc tcggatgaga ttagaatatc ccagaccacg
     3421 ccccctggca cagccttgta cctcgcacgt gcggaagaca gagacagtgg gcggaacgga
     3481 ctcatccggt actccatcgc cagcccgcag ccaggcgtct ttgccatcga cagagccctg
     3541 ggggtgctgt tcctcaacgg cagcctgggc gcgggcgagc agcgggagct cacgctgact
     3601 ctcagggccg aggaccaagg cgtgcatcct caggcagccc tgctggtgct gacagtcgtt
     3661 atcgagaaac gcgaacacag cccatcctgg actttcgaac atttggtcta tcaagtggaa
     3721 gtcagtgagt ctctctcacc gatgacacaa atgctgcaaa cacaggcgca cccacttggc
     3781 ccccagcgtg cagcctcgcc tcttaggtac tcgctggaac ccagcgtaga ctctgctatg
     3841 tttggaatcc gcccttacac gggctggatt tatttgcggc gacagtttga ctatgaatcc
     3901 acccaaacat ataattttag agtgtttgct tggatccccg aggacggatt cttgcaaaat
     3961 gtgagcacta cagtcattgt tcgtgtctgg gatgagaatg acaattcccc caccttcttg
     4021 catgatgtgt tgtttttgaa agtcgaagag agccctgttc cccaaggggt aataggcaaa
     4081 attacagcta ttgacatgga ctctggaaag aatggacagc tattatattt ccttttgtct
     4141 gatggaaaat tcttcaagat gaatcctaat acaggagagt taatcaattg ggtggcactg
     4201 gatcgtgagc accgggggca ccatgagatg actgtgctag tgacagaccg cggctcccca
     4261 ccacgaaacg ccaccatggc ggtttacgtc tcagttactg acatcaatga taacaggccc
     4321 ttcttccccc agtgtctccc tggaaaggag ttacacgtga aggttctgga aggtcaacca
     4381 gtaaatatgt tggttacaac tgtgtttgca aaggatcctg atgaaggaaa taatgcagaa
     4441 gttacatact cagtatcttc agaagatagt tctgatcact ttaagattga cgccaacaat
     4501 ggtgaaataa gaacaaccac aatactttcg tatgattata gaccttccta cagaatgagt
     4561 gtcattgcca ctgaccaggg agtgcctcct cttcaaggac aggcagttgt taatattcag
     4621 gtgatcccac tatccaaagg gagagcaatc atgtctcaga atattagaca tttaattata
     4681 ccagaaaatt tgaagcccac aaaaataatg agcttgataa agtcatctga tcaccttcaa
     4741 caacattata atggaaagtt acattttagt attgttgcag atgataagga tggacacttt
     4801 gaaatagaca gctcaaccgg agacttgttt ctttctaagg aacttgatta tgagacgaca
     4861 tctcattatc ttttcagagt gattactaca gaccatagca aaaacctttc cctgagtagc
     4921 acagtcttcc ttagtatcga tgtggaagat cagaatgacc attccccatc tttccaggat
     4981 gagctcattg tgatcagtgt agaggagaat gttcccatag gaaccctggt gtatgtcttc
     5041 aatgccaaag atgatgacgg cagttttttg aacagtagaa tacaatacta cattgaatcc
     5101 cacaaccctg gcacgaatcc atttctcatc cacccctcat ttggcacact agtcactgtg
     5161 tcccgtcttg acagagaaag cattccaact gtcatcctga cagtaacagc atctgatcag
     5221 gctgtgaatg tgacagaccg gcgactgaga tcactgacag cacaaatagt gattttggat
     5281 gtaaatgacc acaaccccac ttttatttct ttccccaatg cccatgtcaa agaggatgtc
     5341 acagtgggct ccttggtcca ccacataact gctcacgatc cagacgaagg aaggaatgga
     5401 aaagtaacat acagcatcct ctcaggaaat gaaaacatga cgtttatgct agatgagtca
     5461 tcaggcttac taaccacaac ctgtcctttg gattatgaaa tgaaaactca gcatattctg
     5521 actgttctgg cactggatga tggcacacca gcactttctt catcccagac tttgacagtt
     5581 actgttcttg atgtaaatga tgaagctcca gtatttaagc agcacctgta tgaagcctca
     5641 gtgaaagaaa accaaaatcc aggggagttt gttaccaggg ttgaagctct ggacagagat
     5701 tcaggagtca attccaagct tcagtttgaa atcatgccag gtgcttcatt tgaattattc
     5761 gagataaatt ctgacactgg agaggtagtg acaaccacca tacttgacag agaaattcaa
     5821 gaagtcttca cccttcgagt actagtacga gatgggggat tcccttcatt gtccagcacc
     5881 acaacaatcc tctgcactgt tgaagatgaa aacgatcacg caccagagtt tattgtttcc
     5941 agttatgaca ttgaggttct ggaaaaccag gaaccagagg ttgtctatac ggttttagcc
     6001 tctgatatgg atgctggcaa taacagagct gttgaatatc acataattga tggaaatact
     6061 gatgagtgct ttactataaa tgagatgtca ggagaactct caaccactcg tgctttggac
     6121 cgggagcaaa tcagcaattt tacccttgtc attctgtgct ctgacctggg agatccacct
     6181 aggagctctg taatacacct gcaagttaga gttttggatg ccaatgacca cagtccttct
     6241 tttcccacac tttattacca gtcctctgtg agagaagatg ctgaagtggg aacagtggtt
     6301 cttgtgcttt cagctgtgga caaggatgaa ggcctgaatg ggcaaactga gtattttctg
     6361 actgatgagg cttctggtgc attcaccatt gatcctatgt caggcacatt gaaaaccagc
     6421 aacaccctcg accgtgaagc cagatctcag catacattta gtgctgtggc cagagactgt
     6481 agcatccagg gttcacgaag caccactgta attataaaag tatatgtcac tgatgttaat
     6541 gacaatgatc cagttttgga acagaaccct tttgatgtgt ttctttcccc cgagtcgcct
     6601 acaaaccaga caactgtcat tgtgagagct gatgacctgg acttggggcc caatggaact
     6661 gtggttttta gttttgcaga gacccagtca atgttttcta ttgataaata cacaggagaa
     6721 attcaatttc agcaaaatcc atcttcagaa tacttcccta tctggctgca gttaaaagtt
     6781 acagaccagg gcattccagc caggacaacc acgggtctct tggtcattca catggaagga
     6841 gaagatgtaa agatttcctt cagccaccac ctgtataaag ggctcgtgac tgaaaattgt
     6901 gaggcaggta cttctattgt gactgttaaa gcttttgctc ctgactcaat tcaggacagc
     6961 atgaaatatt caatttttag tggaaatgaa gatggagttc tttccctgtg ctctaagtca
     7021 ggacaactca ctgtgaaaga acccaagttt cttgattttg aagtcagaaa tgaggttcaa
     7081 ctcatcgttt tagctgaaag tagtgggcat agagcctatt gcaaagtagc agtcttgata
     7141 caggatgaga atgataattc accatgcttt gaacaaagca tttaccaggc atcagtgtct
     7201 gaaagccaac tctacaatgc tcatgtcata caggtttttg ctaccgactt ggacagtggt
     7261 ttgaacggcc tgattgagta ttctattctg tctggcaacc aagaagaagc attccagatt
     7321 gatgcactga gtggtgtgat aacaacaaaa gcgattctag attacgagct caccagctcc
     7381 tacagcttga ttgtccaagc cacagataaa gggatgccca ggctttctaa tacgactgta
     7441 atcaaggtac aggtgactga tataaatgac aatgccccag cttttctccc ctctgaagca
     7501 gtggaaatta cagaagattc tttgcctggt gtaattgtga ctcatgtgtc agttcatgat
     7561 gtggatttga attcagcttt catattcagt tttgccaaag agagtaatcc tggaaccaag
     7621 tttgctattg atcagaacac tggagtggtg gtgttggtga aaacattgga ttttgaagaa
     7681 atgactgaat atgagctgct catccaaatt tctgattcag tgcactacac agagggagca
     7741 cttgtagtcc gtgtgctgga tgtcaatgat aatccaccag tgttttctca agatttctat
     7801 caggtcacag ttcctgaatc aatacctgtg gggtattcag tgctgactct gtcagccaca
     7861 gacttagaaa gcaatgagaa catttcttac agaattctat cctcttctaa ggaattctcc
     7921 attgatccta agaatggcac aatatttact atcagtcccg tattacttct ggatacaata
     7981 tcaacaactc aatttcttgt ggaagccagt gatggtggaa atcctgacct gagagctctt
     8041 actttagtgg agataggaat agaagatatg aacaattatg cccctgaatt cacagtcaaa
     8101 tcctataatc ttagcctaag tgaggatgct ctggttggaa gcacgcttgt tacattttca
     8161 aacatcgacc atgactggac ccgtgaaaac acatatgttg aatattccat catcagtggt
     8221 aattcacaga acaattttca tgtggaaact aagttctttc attcagaata tccttataag
     8281 caagtcggtt atcttgtgtt gcttcacagt ctggacagag aagcaagtgc tagccatgag
     8341 cttgtcattc tggcatctga cagtggctgc cctccattga gttccacagc tgtcatatca
     8401 atacaagtac ttgatgtcaa tgacaatccc ccaaacttca gcagcctgag ctatcacacc
     8461 catgtcaagg aaagcacccc tctagggagt cacatcactg tggtctcagc aaatgaccgt
     8521 gacacagggt cacatgcaga aatcatctac aacatcatct ctggaaatga gaagggacat
     8581 ttttacttag aagaaaacac tggagttctt tatttgatta aacctctgga ttatgaaaaa
     8641 atgacaaaat tcaccttaac tgtccaagct tcagatgcag aaaagaaaca tttttctttt
     8701 gcagttgtgt ttgtcagtgt cctggatgat aacgaccatg cacctcagtt tatgttctca
     8761 agcttcagct gtattgttcc agaaaatctg cctatttcct ctaccatatg ctctataaat
     8821 gctctggatt ttgatgctgg tccgtatgga gaattgacct attctattgt atcaccctgt
     8881 tttctcactc atggaatgtc ttatgatcat gatctcttcc tcattgaccc tttgacaggg
     8941 gatattcatg ctaagcaaat ccttgactat gaaaatggca ataaatactg cctcacagtc
     9001 caagccaaag acaaaggtga tgcaactgcc tccttagtgg tctgggtgga tattgaaggg
     9061 atagatgaat ttgagcccat tttcactcaa gatcagtatt ttttcaccct cccagaaaag
     9121 aataaagaca gacagttgat tggcagagtg gaagcctcag atgcagatgc tggtattgat
     9181 ggagtcattc tttactccct tggaacctca tctcctttct tttcagtaaa taaaaccaat
     9241 ggaaatattt atttgattag agcccttccc ctaataaaaa gtcaactcaa caaagaagac
     9301 accttggaaa tgaaaataat cgctcatagt cccaaatcag attccaagtt tgcatcttgc
     9361 actgtttttg tgaatgtgtc tttctcctct gaaggaacac ccttggcagt gttcgccagc
     9421 agcttttcaa tcagcctggt ggtctccttt ttagtgtttc tgatactcat ctgcattcta
     9481 attgtaatga ttttaagaca taaacaaaaa gacacaataa acaattatga ggagaagaaa
     9541 acctcatctt tagatgcgga cttgagagtg acccgggatg ccagtgtgct caaagccttc
     9601 cagaaaactg acgactgcag taacgaggtg gtccctgtgg atgccactcc ggaatggttg
     9661 agtttaataa gtatcatgga gaaggatatt gtcaatctgt acagacactc aaactccagt
     9721 ggccactgtt ctgtggaagg agaaactgca gaagataagg aaatccagag gataaatgag
     9781 catccctaca gaaagtgctc agactcagct ctgagtgacc acgagtccag ggtgccagac
     9841 tcgggtatcc cgagggactc agaccagctc tcctgcctat ctggggaaac tgatgtgatg
     9901 gtgactgccg aaacagcaga agccagccaa acatttgggg aaggagatca aggggaaggc
     9961 tgcagcacca cctgtgctca aaataatgtg ttaccccaga cagttcagaa gagagaggca
    10021 aaagagagca tcctggctga cgttagaaaa gagtctgtct ttatttcagg tgatcaggaa
    10081 gtaaggtgtg cagctctttc aactcagacg acctctgatc atgatggaaa agacaactat
    10141 cactggaatt atcttcttag ttgggagccc aaattccaac ctcttgcctc agtatttaat
    10201 gatattgcaa aactaaagga tgaacatttg catatgcctg gcattccaaa agagaagaaa
    10261 tcttttgttt ttccaccccc tttgataaca gcagtagccc agcctgggat taaagcagtc
    10321 ccaccaagaa tgccggcagt aaacctgggg caggtgcctc cgaaacaccc acgctctccc
    10381 atcccctacc atcttggttc tctgccagaa ggcatgactc ccaatttttc tccatctctt
    10441 tccctattga cgatgcagcc tcctgccttg tctccactgt tgagagaagg agaattatta
    10501 ggaacacaca tcagtggtac atgccatgaa cttaaagcag aagatgaagt tcaaatatga
    10561 aaccactggg atgccaagta cctgctcacc attggtcatg aatgaatgaa caaaatgttt
    10621 tcaagccagc aactcgagat tgggctcatt tttatctaaa agcaagtgat gtaatttagt
    10681 tagagttttt aaaacttccc cattaaagtt tctccaattt cttccagcct taatgaaaag
    10741 ttatttcctc ttttaatcct tatgtgttgc tctcaggact cacatttgta tatattaagt
    10801 agtgtatatt ctgtcagacc tgttgtctgt atgattagac ttccaaagga cacagtgaac
    10861 ttaactgact gccagaatgt ttatatggaa acaaaatata gtgtactctg gtcacattgc
    10921 tgctgatcac actgctatat caaaaacaaa ctagtaaagg ctaaatggag aatgagttta
    10981 ggtcagaaca tatagcagag aggggaagtc tagaggaata aactttcatt ttaagataaa
    11041 gcaatagttg gtggatctga tatatttaga ggagtatttc tccatttttc cccatatacc
    11101 acacctgtag agtttagcca taattgaatt tcaatgtgga gtcttttgga aaactaataa
    11161 aaaattcaga aggaaatttg tcataactct gtgacaatag ggagaaacaa ataggattta
    11221 taatgatagg taatataaat aattattcat gattcaataa accagaaatg catgttcctg
    11281 atagagggcc aggattgata ctcgcaatat ttatgtggtt tgctaccacc tgaaacatat
    11341 tatcttggcc ttctttacaa acagaatggc ttttttttca ggtcttgtct tataagtagg
    11401 tgtgggtaaa gacattaaac ttcaaaggtt aatttatagc aatgtcttca ttttattttc
    11461 ttctagatac agtaactttt ttgtgtgaaa taatttatcc actcaatgtc agtttttaaa
    11521 atttgtgtag ataaaaatca tgtcattagc taatgataaa tctcatagag taatttagca
    11581 atatattttt aaatgaagtt ttttatggaa atgtttctac tataaaagtt aagaatagaa
    11641 gtcagaagtc tttttaccat ttgctcacac acttgcagct ttaatttaac tgtcattaga
    11701 tggagcagtt tggcacatgg ttaaaatgac tctgtacaat tactatttta gaaatatagc
    11761 agtgctagat ggtgctcaca tacaatgctc tagccttctt tctgataagc ctgggaatcc
    11821 ctcacagatt ctcatccctt tcaggaaggt ggacagcatc ctgacgatgg tggttcagaa
    11881 gtcctcttcc tgtggtcagc atgagggcca ggtgttaggc taacagttta ggaattctaa
    11941 tgtgaaatct aaagggtatc gtgtggcttt cttagtagtt aggatgggct tctactacag
    12001 atatagttcc tttgtgttct tcttccatag gcctcatctg tgaaattttg tgacataatt
    12061 tctctttact aaaaaatatt gcaaggtgca gtgtttgatg tctggttttg tagataagta
    12121 attctacagc ttcctatgct gatattttct attatctatc tatgctgact gagattggga
    12181 acaaatgtat aaagctatat tacaaattgt atgaaacatg aaggtttttg aataagcctg
    12241 aaatcctagg aaaaatcctt taggagtaga aaatttaaat actatgggac tgatatatga
    12301 tgtttctcct tttcttcctg gcatctgggg aagaagattt catgacttat gtaaaaaaaa
    12361 aacccacaca tatttaatca tttatgtgtt ccagtattta ttatctctga ctcttttgaa
    12421 aacattgctt tgagcataga aagaaaccct atactatatt ttcctaatta tatatggaag
    12481 ttttcttttc acaatagaat tatttctgga aactaagaac attttgttgt ttttaattaa
    12541 acaggttctt catttactta tgcttaaaaa gagttattca tctacctatt ccctaagtta
    12601 gttatttatt actgtgattt atttactcat tatggatgtg attctgtatg ttttctagcc
    12661 attgcataac aagagaagac atgtaataga gcttttttac caatatttaa tggattttta
    12721 attgatgggt ttatcagtct gtatccagtg ccacaatgcc accaaacaag tactacaagt
    12781 gggaatgggg ttaaaaatgt gaacacgctg gttaagtgtt tgcaataaat cgtatgtcag
    12841 ctctttcaac tagagtgctt tttttcctaa gtaaacaata taatttgtac atttttcctt
    12901 caagtttaat attaacttgc ctcatatgtt ctgggttact ttgctgatct atgcactggc
    12961 tttagcactc tggagggggt acagaacttg ttagtggaaa atcatattaa gccccaaatt
    13021 cataagctat aaaataaaag acagttgtct cttgtggaat tttggctgcc tgatgaaatt
    13081 ctggactttc ttgttggcag ccttaaaaat actggtgtaa atcagtacca aatggatatc
    13141 aaaattccat gtcctagtag taatcattga attggcttcc aaaacagatt cctgggcaaa
    13201 gaaaaagaaa aatgctgtat ctttagggat ttgatcattt aactctttta aaaattatta
    13261 aaatgcactc tacattactg gctgcctcca tgtattcata ttcaatctat aatagacgtc
    13321 cagttgagac aggatttgtg tttctcagca cctt
//
