LOCUS       NM_015726               3867 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DDB1 and CUL4 associated factor 8 (DCAF8), transcript
            variant 1, mRNA.
ACCESSION   NM_015726
VERSION     NM_015726.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Liu,F., Han,Q., Zhang,T., Chang,F., Deng,J., Huang,X., Wang,W.,
            Xu,Y., Li,Q., Xu,L., Zhang,B., Li,W., Li,L., Su,Y., Li,Y. and
            Shao,G.
  TITLE     CRL4-DCAF8L1 Regulates BRCA1 and BARD1 Protein Stability
  JOURNAL   Int J Biol Sci 18 (4), 1434-1450 (2022)
   PUBMED   35280675
  REMARK    GeneRIF: CRL4-DCAF8L1 Regulates BRCA1 and BARD1 Protein Stability.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3867)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3867)
  AUTHORS   Huang,D., Liu,C., Sun,X., Sun,X., Qu,Y., Tang,Y., Li,G. and Tong,T.
  TITLE     CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid
            leukemia factors (MLFs)
  JOURNAL   Biochem Biophys Res Commun 529 (2), 127-132 (2020)
   PUBMED   32703400
  REMARK    GeneRIF: CRL4(DCAF8) and USP11 oppositely regulate the stability of
            myeloid leukemia factors (MLFs).
REFERENCE   5  (bases 1 to 3867)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3867)
  AUTHORS   Li,M., Wang,L., Ren,X. and Zheng,C.
  TITLE     Host cell targets of tegument protein VP22 of herpes simplex virus
            1
  JOURNAL   Arch Virol 156 (6), 1079-1084 (2011)
   PUBMED   21424732
  REMARK    GeneRIF: while RNF10 and WDR42A or VP22 alone showed distinct
            subcellular localization patterns, RNF10 and WDR42A were relocated
            when co-expressed with VP22 or its homologues; these potential host
            cell factors of VP22 might expand the list of host targets of VP22
REFERENCE   7  (bases 1 to 3867)
  AUTHORS   Li,T., Robert,E.I., van Breugel,P.C., Strubin,M. and Zheng,N.
  TITLE     A promiscuous alpha-helical motif anchors viral hijackers and
            substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery
  JOURNAL   Nat Struct Mol Biol 17 (1), 105-111 (2010)
   PUBMED   19966799
REFERENCE   8  (bases 1 to 3867)
  AUTHORS   Venkatesan,K., Rual,J.F., Vazquez,A., Stelzl,U., Lemmens,I.,
            Hirozane-Kishikawa,T., Hao,T., Zenkner,M., Xin,X., Goh,K.I.,
            Yildirim,M.A., Simonis,N., Heinzmann,K., Gebreab,F., Sahalie,J.M.,
            Cevik,S., Simon,C., de Smet,A.S., Dann,E., Smolyar,A.,
            Vinayagam,A., Yu,H., Szeto,D., Borick,H., Dricot,A., Klitgord,N.,
            Murray,R.R., Lin,C., Lalowski,M., Timm,J., Rau,K., Boone,C.,
            Braun,P., Cusick,M.E., Roth,F.P., Hill,D.E., Tavernier,J.,
            Wanker,E.E., Barabasi,A.L. and Vidal,M.
  TITLE     An empirical framework for binary interactome mapping
  JOURNAL   Nat Methods 6 (1), 83-90 (2009)
   PUBMED   19060904
REFERENCE   9  (bases 1 to 3867)
  AUTHORS   Jin,J., Arias,E.E., Chen,J., Harper,J.W. and Walter,J.C.
  TITLE     A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2,
            which is required for S phase destruction of the replication factor
            Cdt1
  JOURNAL   Mol Cell 23 (5), 709-721 (2006)
   PUBMED   16949367
REFERENCE   10 (bases 1 to 3867)
  AUTHORS   Doudney,K., Murdoch,J.N., Paternotte,C., Bentley,L., Gregory,S.,
            Copp,A.J. and Stanier,P.
  TITLE     Comparative physical and transcript maps of approximately 1 Mb
            around loop-tail, a gene for severe neural tube defects on distal
            mouse chromosome 1 and human chromosome 1q22-q23
  JOURNAL   Genomics 72 (2), 180-192 (2001)
   PUBMED   11401431
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139011.20.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_015726.3.
            
            Summary: This gene encodes a WD repeat-containing protein that
            interacts with the Cul4-Ddb1 E3 ligase macromolecular complex.
            Multiple alternatively spliced transcript variants have been found
            for this gene. [provided by RefSeq, Jul 2009].
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.1930.1,
                                           SRR11853560.2262.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368074.6/ ENSP00000357053.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AL139011.20        48387-48487         c
            102-175             AL139011.20        47223-47296         c
            176-250             AL139011.20        29898-29972         c
            251-924             AL139011.20        25635-26308         c
            925-1065            AL139011.20        24545-24685         c
            1066-1160           AL139011.20        23073-23167         c
            1161-1271           AL139011.20        17235-17345         c
            1272-1344           AL139011.20        11529-11601         c
            1345-1402           AL139011.20        11000-11057         c
            1403-1510           AL139011.20        10380-10487         c
            1511-1641           AL139011.20        8589-8719           c
            1642-1761           AL139011.20        4787-4906           c
            1762-1878           AL139011.20        4262-4378           c
            1879-3867           AL139011.20        1658-3646           c
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.2"
     gene            1..3867
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /note="DDB1 and CUL4 associated factor 8"
                     /db_xref="GeneID:50717"
                     /db_xref="HGNC:HGNC:24891"
                     /db_xref="MIM:615820"
     exon            1..101
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            102..175
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            176..250
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..1995
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /note="WD repeat domain 42A; WD repeat-containing protein
                     42A"
                     /codon_start=1
                     /product="DDB1- and CUL4-associated factor 8"
                     /protein_id="NP_056541.2"
                     /db_xref="CCDS:CCDS1200.1"
                     /db_xref="GeneID:50717"
                     /db_xref="HGNC:HGNC:24891"
                     /db_xref="MIM:615820"
                     /translation="MSSKGSSTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEAS
                     DLSLSLTGDDGGPNRTSTESRGTDTESSGEDKDSDSMEDTGHYSINDENRVHDRSEEE
                     EEEEEEEEEEQPRRRVQRKRANRDQDSSDDERALEDWVSSETSALPRPRWQALPALRE
                     RELGSSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVV
                     VWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNT
                     KRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGL
                     YTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKKFCPHHLVNSESKANITC
                     LVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFV
                     VSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPT
                     AEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGV
                     GATDADSDESPSSSDTSDEEEGPDRVQCMPS"
     exon            251..924
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1065
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1160
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1271
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1344
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1402
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1510
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1641
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1761
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1878
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..3867
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2907..2912
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /note="hexamer: AATAAA"
     polyA_site      2928
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /note="major polyA site"
     regulatory      3846..3851
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /note="hexamer: AGTAAA"
     polyA_site      3867
                     /gene="DCAF8"
                     /gene_synonym="GAN2; H326; WDR42A"
                     /note="major polyA site"
ORIGIN      
        1 agtcttctcg agcacatcgt cgcaaacggg gccggaaagc gtggcagcgc aggcgcaagc
       61 gcagagagcg gaggcggtgg tggtggcggc cgctggccag ttccttcagt gaatctacag
      121 acctattttc tcaggagctc agcctggcct tacttcagtg ataaaaggag gaaaggctgg
      181 ctacagcaaa catcattcaa gatgtccagc aaagggagca gcacagatgg cagaacagac
      241 ttagctaatg gaagcctgtc tagcagtcca gaggagatgt ctggagctga agaggggagg
      301 gagacatcct caggcattga agtggaggcc tcagacctga gtttgagctt gactggggat
      361 gatggtggcc ccaaccgcac cagcacagaa agtcgaggca cagacacaga gagctcaggt
      421 gaagataagg actctgacag catggaggac actggtcatt actccattaa tgatgaaaat
      481 cgagtccatg accgctcaga ggaagaggaa gaggaggaag aagaggagga agaagagcag
      541 cctcggcgcc gtgtacagcg caagcgggct aaccgtgacc aggactcatc agatgatgag
      601 cgggccctag aggactgggt gtcctcagaa acatcagctc taccccgacc tcgctggcaa
      661 gccctccctg cccttcggga gcgggagctg ggttcaagtg cccgctttgt ctatgaggcc
      721 tgtggggcaa gagtctttgt gcagcgtttc cgcctgcagc atgggcttga gggccatact
      781 ggttgtgtca ataccctgca ctttaaccag cgcggcacct ggctggccag tggcagcgat
      841 gacctgaagg tggtggtgtg ggattgggta cggcggcagc cagtactgga ctttgagagt
      901 ggccacaaaa gtaatgtgtt ccaggccaag tttcttccta acagtggtga ttctactctg
      961 gccatgtgtg cccgtgacgg gcaggttcga gtagcagaac tgtctgccac acagtgttgc
     1021 aagaatacaa aacgtgtggc ccagcacaag ggagcgtccc acaagttggc actggaacca
     1081 gactctccct gtacgttctt atctgcaggt gaagatgcag ttgttttcac cattgacctg
     1141 agacaagacc gcccagcgtc gaaactggtg gtgacaaaag agaaagagaa gaaagtgggg
     1201 ctgtatacga tctatgtgaa tcctgccaat acccaccagt ttgcagtggg tggacgagat
     1261 cagtttgtaa ggatttatga ccagaggaaa attgatgaga atgagaacaa tggagtactc
     1321 aagaagttct gtcctcatca cctggtgaac agtgagtcca aagcaaacat cacctgtctt
     1381 gtgtacagcc acgacggcac agagctcctg gccagttaca atgatgaaga catttacctc
     1441 ttcaactcct ctcacagtga tggggcccag tatgttaaga gatacaaggg ccacagaaat
     1501 aatgccacag taaaaggcgt caatttctat ggccccaaga gtgagtttgt ggtgagcggt
     1561 agtgactgtg ggcacatctt cctctgggag aaatcatcct gccagattat tcagttcatg
     1621 gagggggaca agggaggcgt ggtaaactgt cttgagcccc accctcacct gcctgtgctg
     1681 gcaaccagtg gcctagacca tgatgtgaag atctgggcac ccacagctga agcttccact
     1741 gagctgacag ggttaaaaga tgtgattaag aagaacaagc gggagcggga tgaagatagc
     1801 ttgcaccaaa ctgacctgtt tgatagtcac atgctgtggt tccttatgca tcacctgaga
     1861 cagagacgcc atcaccggcg ctggcgagaa cctggggttg gggccacaga cgcggactct
     1921 gatgagtctc ccagctcctc agacacatcg gacgaggagg agggccctga ccgggtgcag
     1981 tgcatgccat cttgaggcct catacctagg tggggcaggc tggggctgcc aacctgatcc
     2041 tgcctgggca accctttcct gtcccaggcc ctacattcag cagaaacgca ctttggactt
     2101 tttgctttag ataaaagaaa gacatcccag gagaaggaca aaccagagga gtgaaccaac
     2161 aaagagtacc taggaatggg agttgagccc tggaatgggg ctctatggag aggtgcatag
     2221 gactcggcag aaatggcctc tccccaaagc ctctttttga gaggagaggg aagcctattt
     2281 tgttaactgg tttgggatag ggaatggggt ttctttttct ttaatctccc ttgtttcttg
     2341 ggctggggga ggggtggggg gaacaactgg ctattcagta ccaaggggcc agagtggagg
     2401 gtaggagtgc cactctctct ttggtttagg tttttgacct tttcttcctt tgttttttaa
     2461 aagtttatga cagttggctc cccccccacc cccagcaacc ccatcccaga atcctatttt
     2521 cctgggaagt ccttaaagcc cctaaccatc ccacactctt cactttcctt tccaccttat
     2581 tcattctctg tacttaccac agtattttgc acttgattac atatccttca ctctcttctc
     2641 ttcatcccat caccccctaa ataggtcagg tgagggaggc tgggaagagg tgggaggagg
     2701 ggcagaagtg aaggaagaat aggaaggata ttacctcttc tgttattttt ttaagaaaca
     2761 ttgtttggtg gcagcaatct ccctgtccct atcactgtta gaggcctaat tttatatcta
     2821 taaatatatt aaaaagcaag tcaaacttgg atgtatcaag gtaaaattat tgtcaaagtt
     2881 taaataccta tatattctct gaatgcaata aagggactta agagtgaaca agagtaatgg
     2941 tgtggaagtg acacctgggg tcagtttacc tctgtgtatg gtcactagag attgggactt
     3001 accctttagg ttttaggagg cttgagaatg gaaggatcct catttctgcc cttcctggtt
     3061 ccctgctttg gtgtaggggt tgggaaaaac aggaaattcc tctcagctct gcctcagatc
     3121 tcctacctct ccttaagtct tgtagggggt tccaaggatg gctcttctaa ccagaggctg
     3181 gcctgtcttt aaaacttaac tactttaggg tggtgccacc actgcagact attgtggtac
     3241 tttgtgacag aagacatgta cacacacacc acacacatac atacacactc tctcactctg
     3301 tctctcttac ctttagctgc ttgatcatta agccatccaa cttcatgcca gttcccttct
     3361 ttatagaaga gtgaagggaa agacttcctg ggtttgactt aaaccttgtc cacctcttga
     3421 tattttagga ttgaggaata agtcattaat ctaaggactg attacagtgg ctggagcttg
     3481 ggcacttgtc ttatcactgg tcactgagtc tgaaagtccc agctgaattc ttgcccttaa
     3541 gtgcttttgc tgctattttt ttgcccccag ttccacaaga tccaaccaag aattctgtat
     3601 cctgggacag tcagattctt ctaaatcagg ccaggaagga ggggaaaaga gtgagagaat
     3661 ggtattccca gatacttctt cctcctgccc cttttcccag cagctctgag accagatgtt
     3721 ggctgctgta cttactccct gaggtaggga atgtgtggtg atcgagtggt ctgtgttcct
     3781 attgctggtg gggtgatagg gtgggctaaa aaccatgcac tctggaattt gttgtatttt
     3841 ctcccagtaa agcttttctt ctcccga
//
