LOCUS       NM_003861               5946 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens DDB1 and CUL4 associated factor 5 (DCAF5), transcript
            variant 1, mRNA.
ACCESSION   NM_003861 XM_031102
VERSION     NM_003861.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Radko-Juettner,S., Yue,H., Myers,J.A., Carter,R.D., Robertson,A.N.,
            Mittal,P., Zhu,Z., Hansen,B.S., Donovan,K.A., Hunkeler,M.,
            Rosikiewicz,W., Wu,Z., McReynolds,M.G., Roy Burman,S.S.,
            Schmoker,A.M., Mageed,N., Brown,S.A., Mobley,R.J., Partridge,J.F.,
            Stewart,E.A., Pruett-Miller,S.M., Nabet,B., Peng,J., Gray,N.S.,
            Fischer,E.S. and Roberts,C.W.M.
  TITLE     Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing
            SWI/SNF
  JOURNAL   Nature 628 (8007), 442-449 (2024)
   PUBMED   38538798
  REMARK    GeneRIF: Targeting DCAF5 suppresses SMARCB1-mutant cancer by
            stabilizing SWI/SNF.
            Erratum:[Nature. 2024 May;629(8012):E12. doi:
            10.1038/s41586-024-07402-3.. PMID: 38684813]
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 5946)
  AUTHORS   Leng,F., Yu,J., Zhang,C., Alejo,S., Hoang,N., Sun,H., Lu,F. and
            Zhang,H.
  TITLE     Methylated DNMT1 and E2F1 are targeted for proteolysis by L3MBTL3
            and CRL4DCAF5 ubiquitin ligase
  JOURNAL   Nat Commun 9 (1), 1641 (2018)
   PUBMED   29691401
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5946)
  AUTHORS   Li,T., Robert,E.I., van Breugel,P.C., Strubin,M. and Zheng,N.
  TITLE     A promiscuous alpha-helical motif anchors viral hijackers and
            substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery
  JOURNAL   Nat Struct Mol Biol 17 (1), 105-111 (2010)
   PUBMED   19966799
REFERENCE   5  (bases 1 to 5946)
  AUTHORS   Jin,J., Arias,E.E., Chen,J., Harper,J.W. and Walter,J.C.
  TITLE     A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2,
            which is required for S phase destruction of the replication factor
            Cdt1
  JOURNAL   Mol Cell 23 (5), 709-721 (2006)
   PUBMED   16949367
REFERENCE   6  (bases 1 to 5946)
  AUTHORS   Lynch,R.A., Piper,M., Bankier,A., Bhugra,B., Surti,U., Liu,J.,
            Buckler,A., Dear,P.H. and Menon,A.G.
  TITLE     Genomic and functional map of the chromosome 14 t(12;14) breakpoint
            cluster region in uterine leiomyoma
  JOURNAL   Genomics 52 (1), 17-26 (1998)
   PUBMED   9740667
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA058347.1, AB058727.1, BX648006.1, BC136632.1, BC013239.1,
            R41943.1 and AL391262.3.
            
            On May 17, 2019 this sequence version replaced NM_003861.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.45730.1,
                                           SRR1660803.91249.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000341516.10/
                                                  ENSP00000341351.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                DA058347.1         79-165
            88-2198             AB058727.1         1-2111
            2199-2987           BX648006.1         2081-2869
            2988-3025           BC136632.1         2995-3032
            3026-4704           BC136632.1         3034-4712
            4705-5894           BC013239.1         965-2154
            5895-5907           R41943.1           15-27               c
            5908-5946           AL391262.3         108726-108764
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.1"
     gene            1..5946
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /note="DDB1 and CUL4 associated factor 5"
                     /db_xref="GeneID:8816"
                     /db_xref="HGNC:HGNC:20224"
                     /db_xref="MIM:603812"
     exon            1..355
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..2970
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /note="isoform 1 is encoded by transcript variant 1;
                     breakpoint cluster region protein, uterine leiomyoma, 2;
                     WD repeat-containing protein 22"
                     /codon_start=1
                     /product="DDB1- and CUL4-associated factor 5 isoform 1"
                     /protein_id="NP_003852.1"
                     /db_xref="CCDS:CCDS32106.1"
                     /db_xref="GeneID:8816"
                     /db_xref="HGNC:HGNC:20224"
                     /db_xref="MIM:603812"
                     /translation="MKRRAGLGGSMRSVVGFLSQRGLHGDPLLTQDFQRRRLRGCRNL
                     YKKDLLGHFGCVNAIEFSNNGGQWLVSGGDDRRVLLWHMEQAIHSRVKPIQLKGEHHS
                     NIFCLAFNSGNTKVFSGGNDEQVILHDVESSETLDVFAHEDAVYGLSVSPVNDNIFAS
                     SSDDGRVLIWDIRESPHGEPFCLANYPSAFHSVMFNPVEPRLLATANSKEGVGLWDIR
                     KPQSSLLRYGGNLSLQSAMSVRFNSNGTQLLALRRRLPPVLYDIHSRLPVFQFDNQGY
                     FNSCTMKSCCFAGDRDQYILSGSDDFNLYMWRIPADPEAGGIGRVVNGAFMVLKGHRS
                     IVNQVRFNPHTYMICSSGVEKIIKIWSPYKQPGCTGDLDGRIEDDSRCLYTHEEYISL
                     VLNSGSGLSHDYANQSVQEDPRMMAFFDSLVRREIEGWSSDSDSDLSESTILQLHAGV
                     SERSGYTDSESSASLPRSPPPTVDESADNAFHLGPLRVTTTNTVASTPPTPTCEDAAS
                     RQQRLSALRRYQDKRLLALSNESDSEENVCEVELDTDLFPRPRSPSPEDESSSSSSSS
                     SSEDEEELNERRASTWQRNAMRRRQKTTREDKPSAPIKPTNTYIGEDNYDYPQIKVDD
                     LSSSPTSSPERSTSTLEIQPSRASPTSDIESVERKIYKAYKWLRYSYISYSNNKDGET
                     SLVTGEADEGRAGTSHKDNPAPSSSKEACLNIAMAQRNQDLPPEGCSKDTFKEETPRT
                     PSNGPGHEHSSHAWAEVPEGTSQDTGNSGSVEHPFETKKLNGKALSSRAEEPPSPPVP
                     KASGSTLNSGSGNCPRTQSDDSEERSLETICANHNNGRLHPRPPHPHNNGQNLGELEV
                     VAYSSPGHSDTDRDNSSLTGTLLHKDCCGSEMACETPNAGTREDPTDTPATDSSRAVH
                     GHSGLKRQRIELEDTDSENSSSEKKLKT"
     exon            356..499
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     exon            500..536
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     exon            537..676
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     exon            677..806
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     exon            807..1020
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1087
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1215
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..5946
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3061..3066
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /note="hexamer: ATTAAA"
     polyA_site      3090
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /note="major polyA site"
     regulatory      5884..5889
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /note="hexamer: AATAAA"
     polyA_site      5904
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
                     /note="major polyA site"
     polyA_site      5946
                     /gene="DCAF5"
                     /gene_synonym="BCRG2; BCRP2; D14S1461E; WDR22"
ORIGIN      
        1 acacctcgct ctgcctgcca tggctggtta aagaaccatc cggatcgcag cgcgggggga
       61 gggccgggtg gggggagcag cggccgcgcg tgaggccgag ggaggggcgg cggcgcgagc
      121 ggcggcggcg gcggttccag catgaagagg agagctggcc tggggggcag catgaggtca
      181 gtggtgggct tcttgtccca gcggggcttg catggggacc ccctgctcac tcaggacttt
      241 cagaggagac gcctgcgggg ctgcagaaac ctctacaaga aggacctcct cggccacttc
      301 ggctgtgtca atgccattga attctccaac aatggaggcc agtggctggt ctcaggagga
      361 gatgaccgcc gggttctgct atggcacatg gaacaagcca tccactccag ggtcaagccc
      421 atacagctga aaggagagca ccattccaac attttttgcc tggctttcaa cagtgggaac
      481 actaaagtgt tctctggagg caatgatgag caagttatcc tccatgatgt tgaaagcagt
      541 gagacattgg acgtgtttgc tcatgaagat gcagtatatg gcttgtctgt gagcccagtg
      601 aatgacaaca tttttgccag ttcctcagat gatggccggg ttctcatttg ggacattcgg
      661 gaatcccccc atggagagcc cttctgcctg gcaaactatc catcagcctt tcatagtgtc
      721 atgtttaacc ctgtggagcc caggttgttg gccacagcca attcaaagga aggagtggga
      781 ctctgggaca ttcgaaaacc tcagagttct ctcctgcgct atggtggaaa cctgtccctc
      841 caaagtgcca tgagtgtacg attcaacagc aacgggaccc agctcctggc cctgaggcga
      901 cgcctgcccc ctgtgctcta tgacatccat tcccgcctgc ctgtgtttca gtttgacaat
      961 cagggttact tcaactcatg caccatgaaa agctgctgtt ttgcaggaga tcgtgaccag
     1021 tatatccttt cgggctctga tgacttcaac ctgtacatgt ggagaattcc tgcagatcca
     1081 gaagcaggtg gcattggtag ggtggtcaac ggagccttca tggtgctgaa agggcatcga
     1141 tctattgtta accaagtccg atttaatccc cacacctaca tgatctgctc ttctggtgta
     1201 gaaaagatta tcaagatctg gagcccatac aagcagccag gatgtactgg agacctcgac
     1261 ggtcggattg aggacgattc ccgctgcctc tatacccatg aagagtacat cagccttgtg
     1321 ctgaacagtg ggagtggcct gtcgcatgac tacgccaacc agtcggtcca ggaagacccc
     1381 cggatgatgg ccttctttga ctcactggta cgccgagaga tcgagggctg gagctctgac
     1441 tcagacagtg acctcagtga gagtactatc ctccaactgc acgctggggt cagcgagcgc
     1501 tcaggctaca ctgactcaga gtcttcggcc tcattgcctc gctccccgcc tcccacagta
     1561 gatgagtctg ccgacaacgc cttccacctg gggcccctgc gggtcaccac cacaaacaca
     1621 gtagcctcaa ctccaccaac acccacgtgt gaggatgcag cctctcgcca gcagcgtctg
     1681 tctgctctgc ggcgctacca agacaaacgc ctcctggccc tttccaatga gtccgattct
     1741 gaggagaatg tctgtgaggt ggaactagac acagatctct ttccccggcc acggtcaccc
     1801 agccccgaag atgaatccag cagttccagc agctctagca gctctgagga tgaggaggag
     1861 ctgaatgaac gccgagcctc tacctggcag cggaatgcca tgcggcgccg acagaagaca
     1921 acccgagaag acaagcccag tgccccaatc aagcccacca acacttacat tggagaagac
     1981 aactatgatt acccccagat caaagtggat gacctctcct cctccccaac ctcgtcccct
     2041 gagcggagca cttccacgct agagattcaa ccaagccggg catcaccaac ttctgacata
     2101 gaatcagttg agcgaaaaat ttataaagct tacaagtggc tccgctactc ttatatctcc
     2161 tactcaaata acaaagatgg agagacctcc ttggtgaccg gggaggcaga tgaagggaga
     2221 gcaggaacca gccacaaaga caacccagcc ccttcttcca gtaaggaagc ctgtctaaac
     2281 atagcaatgg cccagaggaa ccaggacctg ccacctgaag gctgcagcaa ggacactttt
     2341 aaagaagaga ctcctagaac tcccagcaat ggcccaggcc atgagcacag cagccatgct
     2401 tgggcagagg tgccagaggg tacctctcag gacactggca atagcggctc tgtagagcac
     2461 ccttttgaaa ccaagaagct caatggaaag gccctgagca gtcgggctga ggagccgcct
     2521 tctcctcctg tccccaaggc atctggctcc actctcaaca gcgggtctgg caactgtccc
     2581 aggacccagt ctgatgacag tgaggagagg agcctcgaaa ccatctgtgc caaccacaac
     2641 aatggacgct tacaccctcg tccccctcac cctcacaata acgggcagaa cttgggggag
     2701 ctggaggtgg tggcctactc ttccccagga cactcagaca ctgaccgtga taactcgtcc
     2761 ctgacaggga cactcctaca caaagattgt tgcgggtctg aaatggcctg tgagaccccc
     2821 aatgctggaa caagagagga ccccactgac accccagcca cagatagtag cagggctgtt
     2881 catggccaca gtggcctcaa aaggcaacga attgaattgg aagatacaga ttcagagaat
     2941 tcctcctcag agaagaaatt aaaaacatga taaatacaaa gggaaaacaa aaagctacaa
     3001 aagtagcctt acaaaaaaaa aaaaattgtt tagtgaatac agaggaaagg aaaaacaagt
     3061 attaaaggca catgaaacca gggcctgaaa ttttgtgtct gatgctgctc ccttctatcc
     3121 aacattcaac aacgggtctg aatgtctagc tggccattgg cttgtgctgt gttaagaaag
     3181 gggaaaaaaa aataacccaa gtgactcctt tctagtgaga cgtgagcgta gtgtgccgcg
     3241 caaggctctg tggaagtaaa tctttgttga gacgttcgac ttgttcttgt gtacgcacaa
     3301 tggcacgtgt gcgcttattg tctcataaga ccttcctttt agttctccat cagaaaggac
     3361 ctatgaattt gtactttttg ccatctcacc ctgtttacat gccataggtg ttctggtctg
     3421 gatgatgggt gcctgttaaa tcaggttgtg ctgccagtga gttgtagatt tatgctggaa
     3481 gaatggagaa tggaaacagg ccttcagccc attggctgcg ctggagagga aagcatggtt
     3541 cctttcttaa agaaatcttc catgttttct ctaagtaaca cttctttatt gagcatttaa
     3601 tgtggacata ctataaaaaa gaatttttaa tgctaatgag aggtttagca cttaagatca
     3661 gattcaagag aatatagatt cctgataaat ttcactgttt gcaaattgag cagtgctgca
     3721 acatgtactg tatgaaggcc ataaaataga gctagtcatg gataacagac tttaaccagt
     3781 taggtgctag atcctaagag gcactcacaa ggccagcagg tgcttccttg gctcccatga
     3841 tcgatcgagg catagtgaaa gaagggcaaa gacagctttc cagagctttg tgctcaggct
     3901 actaaagtag ttgttatagg tgtgttaacg aagaagctta gtgaggagca gaatgtggta
     3961 ctatattcat ttaactcaac ccagaaccat tggtcagaat tggtgggact ggcatcttcc
     4021 ctcagacttg cagttgatag aggctgtggt gttctcacta gactttgcag agcagtaggc
     4081 atctcaaggt aacagattct tattgagtag gtctattagg agtcagaagg tgttggggag
     4141 gagtctctgg cttatgactc ctggttccca ttagtcttaa gtcttctggt tcccataaaa
     4201 gactcagttt tattgaggta gatacaggtt tagaagagaa aagagcaaca gattttcagc
     4261 gttgcagtca ctcggttatc tatgaagggt tgtgcaaaac aggaaggtct gggaatgacc
     4321 agatatcttg ttggcataaa tgttatgaaa aggcagaata ctggcaagaa atgaggaaga
     4381 gagagaaaaa caatataact ttcttgagtg caaaggtgct agacaactag gagcccagct
     4441 gggattggtg tagggagggc cgaacgctga ctagtgtggc ctcactgccc caggcttcct
     4501 ttcagaggaa ggcctgtcct gctggaactg gagccaaggg gaggctgcct atgaaggcag
     4561 aagatgtgga tgaattcagg cactgggggt gggggggtta tttataaaca caaggtggtt
     4621 ggtttaagcc aaaaggagat accatcctga taagctctgt gaaagagaag atgtgcctgg
     4681 tgtgtgtaat gcggctgggg tatgaaaaca ctgagaagga gatgaaggag tgtgtgtagc
     4741 atgggggtgt gtacaactta acacctggac ttttactggg ggagtgggaa agtgattctg
     4801 taaccaacat tagaattact ttggatttgt aatcagattg gttctatgat ttgtctttga
     4861 ctattcacag ataattgtaa atgctggttt tataagccca agtcatttta catttgcttt
     4921 tccaaaatgt attaaattgg aattttttaa tcctttatat acaaaaaaaa tacgatcact
     4981 cagtgtgtgt ttctcttccc cttcttagtc agcacaccca gtcctttatt ttattctatt
     5041 ctatttattt tattttaata agtacttagc ctcttctgac ttctcaaagg acaatcggat
     5101 acatatcttc ctgaaaagaa gtagttaaag ttcccttggg aactagctac ttggtagtta
     5161 caattcccaa atgcagaact tggtcttttc atgttaaaag ccttaatctc atttgggtac
     5221 tcaaaagaga aatcagagtt tattctggtt aacttgagaa gccaagaaag atcattttta
     5281 gcaagtatgc cttccatcct tggcagaggc ttcccagcat cctaggctca gcttaccggg
     5341 tgataggccc cataagacac acacatgagc agggtgagaa atgcttgctt tatagctgct
     5401 ccaccctcat ctccagagat tttgtgtcta tggttgtggg tctggaaacc tggcaggaac
     5461 cacttctaga gtacctttgc ctaaactctt ttagaattga ggccccaact ttgatggcac
     5521 atttaatacc caatttcttc cataatagat atttttaata gcaggacaga tggcactgat
     5581 tagatcaaga actatttgga agtgcctctt atgttttttt cctgcattct gtacataaag
     5641 ttttaagtgg caggtccaga tatgtaaaat catttctctc tattttccag ctggttttgt
     5701 taatgattct ctaggacagc aggtaaatga gttaagttgt tggcttgggg caagtgaagt
     5761 ttgtgactat gcttgattta ataagctgag agtctcttgt ttgtctgtca aggtgccttc
     5821 ctctgctgaa gccttacctc cttctcaccc agtaacggtt aactcttctc ttgtgtatat
     5881 gcaaataaac ctattattca cttctcacgt cttcctcttc tcttccaggg aagacttggt
     5941 tgctta
//
