LOCUS       NM_017741               4547 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens DDB1 and CUL4 associated factor 16 (DCAF16),
            transcript variant 2, mRNA.
ACCESSION   NM_017741
VERSION     NM_017741.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Pu,C., Tong,Y., Liu,Y., Lan,S., Wang,S., Yan,G., Zhang,H., Luo,D.,
            Ma,X., Yu,S., Huang,Q., Deng,R. and Li,R.
  TITLE     Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for
            triple-negative breast cancer
  JOURNAL   Eur J Med Chem 236, 114321 (2022)
   PUBMED   35430559
  REMARK    GeneRIF: Selective degradation of PARP2 by PROTACs via recruiting
            DCAF16 for triple-negative breast cancer.
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Zhang,X., Thielert,M., Li,H. and Cravatt,B.F.
  TITLE     SPIN4 Is a Principal Endogenous Substrate of the E3 Ubiquitin
            Ligase DCAF16
  JOURNAL   Biochemistry 60 (9), 637-642 (2021)
   PUBMED   33636084
REFERENCE   3  (bases 1 to 4547)
  AUTHORS   Zhang,X., Crowley,V.M., Wucherpfennig,T.G., Dix,M.M. and
            Cravatt,B.F.
  TITLE     Electrophilic PROTACs that degrade nuclear proteins by engaging
            DCAF16
  JOURNAL   Nat Chem Biol 15 (7), 737-746 (2019)
   PUBMED   31209349
REFERENCE   4  (bases 1 to 4547)
  AUTHORS   Jin,J., Arias,E.E., Chen,J., Harper,J.W. and Walter,J.C.
  TITLE     A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2,
            which is required for S phase destruction of the replication factor
            Cdt1
  JOURNAL   Mol Cell 23 (5), 709-721 (2006)
   PUBMED   16949367
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC005768.17.
            
            On Nov 23, 2018 this sequence version replaced NM_017741.3.
            
            Transcript Variant: This variant (2) plus variants 1 and 3-6 all
            encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA153578.1, SRR1803614.233453.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA2162568,
                                           SAMEA2162823 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000382247.6/ ENSP00000371682.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-311               AC005768.17        76958-77268
            312-430             AC005768.17        82490-82608
            431-4547            AC005768.17        82944-87060
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.31"
     gene            1..4547
                     /gene="DCAF16"
                     /gene_synonym="C4orf30"
                     /note="DDB1 and CUL4 associated factor 16"
                     /db_xref="GeneID:54876"
                     /db_xref="HGNC:HGNC:25987"
                     /db_xref="MIM:620524"
     exon            1..311
                     /gene="DCAF16"
                     /gene_synonym="C4orf30"
                     /inference="alignment:Splign:2.1.0"
     exon            312..430
                     /gene="DCAF16"
                     /gene_synonym="C4orf30"
                     /inference="alignment:Splign:2.1.0"
     exon            431..4547
                     /gene="DCAF16"
                     /gene_synonym="C4orf30"
                     /inference="alignment:Splign:2.1.0"
     CDS             1061..1711
                     /gene="DCAF16"
                     /gene_synonym="C4orf30"
                     /codon_start=1
                     /product="DDB1- and CUL4-associated factor 16"
                     /protein_id="NP_060211.3"
                     /db_xref="CCDS:CCDS3423.1"
                     /db_xref="GeneID:54876"
                     /db_xref="HGNC:HGNC:25987"
                     /db_xref="MIM:620524"
                     /translation="MGPRNPSPDHLSESESEEEENISYLNESSGEEWDSSEEEDSMVP
                     NLSPLESLAWQVKCLLKYSTTWKPLNPNSWLYHAKLLDPSTPVHILREIGLRLSHCSH
                     CVPKLEPIPEWPPLASCGVPPFQKPLTSPSRLSRDHATLNGALQFATKQLSRTLSRAT
                     PIPEYLKQIPNSCVSGCCCGWLTKTVKETTRTEPINTTYSYTDFQKAVNKLLTASL"
     regulatory      4519..4524
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCAF16"
                     /gene_synonym="C4orf30"
                     /note="hexamer: AATAAA"
     polyA_site      4547
                     /gene="DCAF16"
                     /gene_synonym="C4orf30"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtagcg tcggcgcggg agctgagcgg agtggccttg ccaccggagg ggaagggctg
       61 ctggcgttag gcctggctgg gtcgtgcggc tgagcgtgag gctagaaaga aaaagcgatc
      121 ctccaagagt gagaacggga gatctgccga ggataacaac ctcccttgtg gccgaagggc
      181 gggggatccc cacacgaggg ctccgcttcg agagacggcg acaaaggaaa gttcgtagcg
      241 cgttccggag gcccggccac ctctgagaag ggagacgacg ctgctgggtc cgctgagcgg
      301 ggcgacccga gcttatatag aattggtgat aggatcacaa cttctgaggt cccagcaaga
      361 gtcctaacat ctagcagctc taccaccagg acaggccccc taaaactaat tccaacaagc
      421 acctctgaag tcttctcatt cacaggttaa ggctaccttt ccagataatc caacagctaa
      481 atcaccagtt tccaagggca tttgcatcac ctatggaaag catcggatcc ttatacacca
      541 gatgttcttg acagctcatc ccgtcattcc cccctgcaca aaagttgcta taccagcgga
      601 caagcaagac aactactgag cttcttttga ttgtcaagta gatataatat cactgccagc
      661 aaaacagcac aaaaagtgtc tcttcacagt atcactacca gttgggacta tgctaagtaa
      721 atcagcttgc ctagatgagg accccttcta ttcttgggta gccaggattt gaaggagata
      781 ctctgacctg tgccagtggt aagtgactaa tacttttaca cactgaactg gtcctggaaa
      841 ctgagatgtc ttctttgtaa gtgaagaata tacaacataa tcttgaagaa cggcacagtg
      901 gtatgggcca caagcatact ttatgtgtgt aacggactga aagacaaatt aatcttggtg
      961 aaaggatttt tcatctctta tttctatttg ccagtgttag tcagtgttct gctggcttag
     1021 attattacct ttttctggtt ccttactgtg ttttattctg atgggtccta gaaatccctc
     1081 tcctgaccac ttgtcagaat cagaaagtga ggaagaagaa aatattagtt acctaaatga
     1141 gagttctggg gaagagtggg attcctctga agaagaggac tctatggtgc ccaacttatc
     1201 gcctcttgag agtcttgcct ggcaggttaa gtgcctttta aaatattcca caacttggaa
     1261 acctttaaat cctaattcct ggttgtatca tgctaaactg ttggatccaa gcacaccagt
     1321 ccatatactt cgagagatag gtctaagact ctcccattgt tcccattgtg tccccaaact
     1381 ggaaccaatt cctgaatggc cccctctggc ctcttgtgga gtcccacctt ttcaaaagcc
     1441 tcttacaagt cccagccggc tctctagaga tcatgccact ctaaatggag cactgcaatt
     1501 tgccaccaaa cagctaagcc gaacattgag tagagccact cccatacctg aatacctaaa
     1561 acagatccct aattcatgtg tttctgggtg ttgctgtggc tggctgacta aaacagttaa
     1621 ggaaacaact cgtactgaac ccatcaacac tacttattct tacactgact tccaaaaggc
     1681 agttaacaaa ctcctaactg catcactgta aagatctacc atttgctctg atatctctga
     1741 tgttgctaat tgagaaagtg gtacaaagtt agtcattaca cagcccacta atgccttctc
     1801 agtcaaactc tcttatgccc tggcaagcca atgaggatac cctttgacaa accctgtgga
     1861 taaaatgata atatcaaggt caggcatggt ggctcacacc tgtaatccca gcactttggg
     1921 aggctgaggc gggtggatct gctgaagtca gaagtttgag accagccttg ccaacacagt
     1981 gaaaccctgt ctctactaaa aataaaaaaa ttagccgggc gcagcagtgc acgcctgtaa
     2041 tccctgctac tcgggaggct gaaacaggag aatcacttga acccaggaga cggaggttgc
     2101 ggtgagttga gattatgcca ctgtactcca gcctgggtga cagagcgaga ctcagtctca
     2161 aaaaaaaaaa aggagaatta gttaagggga ccatattaca aaagatagtt gaatattctt
     2221 cacactcact tctcaccctc cccaccccaa tttactctgc ctttctgaat ggctttaatg
     2281 agatacctga ttattaatga agaaaagtag accaccaccc cttttgttat ccagacttta
     2341 gaacagccat tgttgaaagc aagaagttag ctacccacaa taataccttt ctgagcatac
     2401 tcttctctaa catacatttc tgtggaagta gttgaattaa attgagtaac tacacaaagg
     2461 aaaatggcag ggctatgaat ttctttccca gcttttatat agatgtgcct acatatgaca
     2521 ctctttaggg ttccttttat tttttctttt tttttttttt tgaggtagtt gaattaaact
     2581 gggtaactac acaaaggaaa atggcaggga tatgaatttc tttcccagct tttatataga
     2641 tgtgcctgca tatgacactc tttagagttc ctttcacttt ttcttttttt tggaaggggg
     2701 cggggaggca ggaggagtgc agttttaagc ctagcctata ctactacaaa cttattatgg
     2761 tacttaaaat tacagcatga acgttctata tcggagtctc ccaccaggct gtctctgact
     2821 aaatcagcct ataagatcat cccttgggat taaacaaaca cctgttctgt agggaatgtg
     2881 tttcctgaat cttggtactc agacttttgt caagagtaaa taacatttca aaagatgaat
     2941 ggaaaaatga ttaagagagc atttctgctt tcagcattaa ctgctaatgg attaaaattt
     3001 taatctgtat agcatttcct ggagtaaaag ctcaagtact ttccacatcc attgcaaact
     3061 tttcaggaac ccttctaatt tttttttttt tttttttttt gagacggagt cttgctctgt
     3121 cgcccaggct ggagtgtagt ggcatgatct cagctcactg caagctctgc ctcccgggtt
     3181 cgtgccattc tcctgccctg cctcccgaat agctgggact acaggcgtcc accaccactc
     3241 ctggctattt ttttgtattt ttagtagaga cagggtttca ccgtgttagc caggatggtc
     3301 ttgatctcct gacctcgtga tccacctgcc tcggcctccc aaagtgctgg gattgaaccc
     3361 ttctaatttt aaagaacctt gttattagaa aatctcagcc taatacaatc tgaagttaag
     3421 agttttagca gcattgtttt tctaagtaga tttagctata gattttcttc tggccaaaca
     3481 aggaagagta tatgcccttg taaatgagtc ttgttttgtt tatttaaata gtcagtcaaa
     3541 acgtagaaat cagtatacgt aaaataaaat gcatgagact attaaatctt ttcatatact
     3601 ctacaaataa aatgaaatct gtgtgtggtc ctggttgact gggcatctaa agggaatcag
     3661 aaaagagatt gtgaaaagtt atatatatat cctcttcctt attttagttt tgctttttcc
     3721 tattttccat aattaagtgc cgtttacaaa gtggcatcaa aaaattgaag caggccaggc
     3781 atggtggctc atgcctgtaa tcccaacagt ttgggaggct gagggcaggt ggatcacttg
     3841 agatcaggag ttcgtgacca gcctggccaa catggtgaaa gcccatctct actaaaaata
     3901 taaaaattag ccgggcgtgg tggcatgtgc ctgtaatcgc agctacttgg gaggctaaga
     3961 caggagaatt gcttgaacct gggaggtgga ggttgcaatg agctgagatc gcgccactgc
     4021 actccagcct gggcaacagt gagacaccgt ctcaaaaaaa aaaaaggtta ggaatcacaa
     4081 attgtgtaaa tatatagcaa ctttaagaag agagaagaat gcttataacc tagtgaatat
     4141 ttctgctgag ccttagttgt cttgtctaaa ggaaaaaaga gggaattgta gaataaaagt
     4201 atttgttagt ggaaattatt gacctgaaac catatcatat tgttagtgta actgaatgta
     4261 tcagaaattg ttcctatgta ggtattctgt gaaactggtt tctaaaatgt tacaggaaac
     4321 tgtttccttt ttttttgttt gtttgtttaa gtggaggtgg gaggaggtag ggaagaggtc
     4381 tgaatattaa aatatgttta tgttcatgtg ttcataatac gttccttttt ctctctgtgt
     4441 gtgtataaaa gtgccttgct catgaatgca tttttgctat tcacttcgaa aggaagcctt
     4501 tccccagctt ggcagctgaa taaaccaata gctttgtaca tgctgaa
//
