LOCUS       NM_001387579            5649 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript
            variant 9, mRNA.
ACCESSION   NM_001387579 XM_017007546
VERSION     NM_001387579.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Zhang,J., Shi,Y., Ding,K., Yu,W., He,J. and Sun,B.
  TITLE     DCAF1 interacts with PARD3 to promote hepatocellular carcinoma
            progression and metastasis by activating the Akt signaling pathway
  JOURNAL   J Exp Clin Cancer Res 43 (1), 136 (2024)
   PUBMED   38711082
  REMARK    GeneRIF: DCAF1 interacts with PARD3 to promote hepatocellular
            carcinoma progression and metastasis by activating the Akt
            signaling pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Ghate,N.B., Kim,S., Shin,Y., Kim,J., Doche,M., Valena,S., Situ,A.,
            Kim,S., Rhie,S.K., Lenz,H.J., Ulmer,T.S., Mumenthaler,S.M. and
            An,W.
  TITLE     Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger
            aberrant gene silencing in colon cancer
  JOURNAL   Nat Commun 14 (1), 2140 (2023)
   PUBMED   37069142
  REMARK    GeneRIF: Phosphorylation and stabilization of EZH2 by DCAF1/VprBP
            trigger aberrant gene silencing in colon cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5649)
  AUTHORS   Zhang,C., Jiang,H., Yuan,L., Liao,Y., Liu,P., Du,Q., Pan,C.,
            Liu,T., Li,J., Chen,Y., Huang,J., Liang,Y., Xia,M., Xu,M., Qin,S.,
            Zou,Q., Liu,Y., Huang,H., Pan,Y., Li,J., Liu,J., Wang,W. and Yao,S.
  TITLE     CircVPRBP inhibits nodal metastasis of cervical cancer by impeding
            RACK1 O-GlcNAcylation and stability
  JOURNAL   Oncogene 42 (11), 793-807 (2023)
   PUBMED   36658304
  REMARK    GeneRIF: CircVPRBP inhibits nodal metastasis of cervical cancer by
            impeding RACK1 O-GlcNAcylation and stability.
REFERENCE   4  (bases 1 to 5649)
  AUTHORS   Poulose,N., Forsythe,N., Polonski,A., Gregg,G., Maguire,S.,
            Fuchs,M., Minner,S., Sauter,G., McDade,S.S. and Mills,I.G.
  TITLE     VPRBP Functions Downstream of the Androgen Receptor and OGT to
            Restrict p53 Activation in Prostate Cancer
  JOURNAL   Mol Cancer Res 20 (7), 1047-1060 (2022)
   PUBMED   35348747
  REMARK    GeneRIF: VPRBP Functions Downstream of the Androgen Receptor and
            OGT to Restrict p53 Activation in Prostate Cancer.
REFERENCE   5  (bases 1 to 5649)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 5649)
  AUTHORS   Borgne-Sanchez,A., Dupont,S., Langonne,A., Baux,L., Lecoeur,H.,
            Chauvier,D., Lassalle,M., Deas,O., Briere,J.J., Brabant,M.,
            Roux,P., Pechoux,C., Briand,J.P., Hoebeke,J., Deniaud,A.,
            Brenner,C., Rustin,P., Edelman,L., Rebouillat,D. and Jacotot,E.
  TITLE     Targeted Vpr-derived peptides reach mitochondria to induce
            apoptosis of alphaVbeta3-expressing endothelial cells
  JOURNAL   Cell Death Differ 14 (3), 422-435 (2007)
   PUBMED   16888644
  REMARK    GeneRIF: Hence, this chimeric peptide (TEAM-VP) constitutes the
            first example of a virus-derived mitochondriotoxic compound as a
            candidate to kill selectively tumor neo-endothelia.
REFERENCE   7  (bases 1 to 5649)
  AUTHORS   Jin,J., Arias,E.E., Chen,J., Harper,J.W. and Walter,J.C.
  TITLE     A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2,
            which is required for S phase destruction of the replication factor
            Cdt1
  JOURNAL   Mol Cell 23 (5), 709-721 (2006)
   PUBMED   16949367
REFERENCE   8  (bases 1 to 5649)
  AUTHORS   Zhao,L.J., Zhang,S. and Chinnadurai,G.
  TITLE     Sox9 transactivation and testicular expression of a novel human
            gene, KIAA0800
  JOURNAL   J Cell Biochem 86 (2), 277-289 (2002)
   PUBMED   12111997
  REMARK    GeneRIF: demonstration that a novel human gene, KIAA0800, is
            preferentially expressed in the testis and is transactivated by
            Sox9
REFERENCE   9  (bases 1 to 5649)
  AUTHORS   Zhang,S., Feng,Y., Narayan,O. and Zhao,L.J.
  TITLE     Cytoplasmic retention of HIV-1 regulatory protein Vpr by
            protein-protein interaction with a novel human cytoplasmic protein
            VprBP
  JOURNAL   Gene 263 (1-2), 131-140 (2001)
   PUBMED   11223251
REFERENCE   10 (bases 1 to 5649)
  AUTHORS   Zhao,L.J., Mukherjee,S. and Narayan,O.
  TITLE     Biochemical mechanism of HIV-I Vpr function. Specific interaction
            with a cellular protein
  JOURNAL   J Biol Chem 269 (22), 15577-15582 (1994)
   PUBMED   8195203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092037.2 and AC233756.2.
            
            On Oct 8, 2020 this sequence version replaced XM_017007546.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14372080.3896634.1,
                                           SRR11853563.22942.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000684031.1/ ENSP00000506880.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-143               AC092037.2         118333-118475       c
            144-190             AC092037.2         115194-115240       c
            191-308             AC092037.2         102179-102296       c
            309-385             AC092037.2         89389-89465         c
            386-459             AC092037.2         85263-85336         c
            460-573             AC092037.2         81574-81687         c
            574-711             AC092037.2         62226-62363         c
            712-1224            AC092037.2         59845-60357         c
            1225-1326           AC092037.2         59430-59531         c
            1327-1485           AC233756.2         30775-30933         c
            1486-1665           AC233756.2         27702-27881         c
            1666-1875           AC233756.2         26930-27139         c
            1876-2045           AC233756.2         25041-25210         c
            2046-2170           AC233756.2         19976-20100         c
            2171-3434           AC233756.2         17403-18666         c
            3435-3633           AC233756.2         16347-16545         c
            3634-3716           AC233756.2         15785-15867         c
            3717-3801           AC233756.2         14456-14540         c
            3802-4035           AC233756.2         12293-12526         c
            4036-4129           AC233756.2         11619-11712         c
            4130-4234           AC233756.2         10951-11055         c
            4235-4308           AC233756.2         10662-10735         c
            4309-4410           AC233756.2         10048-10149         c
            4411-4663           AC233756.2         812-1064            c
            4664-5649           AC092037.2         19717-20702         c
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.2"
     gene            1..5649
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /note="DDB1 and CUL4 associated factor 1"
                     /db_xref="GeneID:9730"
                     /db_xref="HGNC:HGNC:30911"
                     /db_xref="MIM:617259"
     exon            1..143
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            144..190
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            191..308
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     CDS             199..4722
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 9;
                     protein VPRBP; Vpr-binding protein; vpr-interacting
                     protein; HIV-1 Vpr-binding protein;
                     serine/threonine-protein kinase VPRBP; Vpr (HIV-1) binding
                     protein"
                     /codon_start=1
                     /product="DDB1- and CUL4-associated factor 1 isoform 1"
                     /protein_id="NP_001374508.1"
                     /db_xref="CCDS:CCDS74943.1"
                     /db_xref="GeneID:9730"
                     /db_xref="HGNC:HGNC:30911"
                     /db_xref="MIM:617259"
                     /translation="MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEK
                     ETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLN
                     TAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDI
                     AANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDY
                     GDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRE
                     NFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYL
                     TPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFV
                     AHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLME
                     CSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDD
                     EIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHE
                     QIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFAL
                     DVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSAL
                     QIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLS
                     LLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDK
                     RSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRN
                     HLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLG
                     SHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKI
                     RVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFS
                     LFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFRE
                     ANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRD
                     GSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYD
                     IQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNI
                     SGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDD
                     LMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDAL
                     NMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAE
                     LEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDN
                     SDLEDDIILSLNE"
     exon            309..385
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            386..459
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            460..573
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            574..711
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            712..1224
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1326
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1485
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1665
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1875
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..2045
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2170
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..3434
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            3435..3633
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            3634..3716
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            3717..3801
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            3802..4035
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            4036..4129
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            4130..4234
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            4235..4308
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            4309..4410
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4663
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     exon            4664..5649
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5625..5630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /note="hexamer: AATAAA"
     polyA_site      5649
                     /gene="DCAF1"
                     /gene_synonym="RIP; VPRBP"
                     /note="major polyA site"
ORIGIN      
        1 ataatatgtg ggccgctgtg gagccgtggt cgcgctgtgt gtgtgtgtga gtggagagtg
       61 agtgcgggac ctgaggctgt gtgtgtgtga actgtggcca ctggcggggt cggggggggg
      121 cagcggcgga gccgcctcgg acgataagga aattgagatt tggagaagtt aaataaccaa
      181 gacttcacag gcaaagccat gactacagta gtggtacatg tggactccaa agctgagctc
      241 actaccctgc tggagcagtg ggaaaaggaa catggcagtg ggcaggacat ggtacctatc
      301 cttaccagga tgtctcaatt gattgaaaaa gaaactgaag agtatcgtaa aggggatcca
      361 gacccatttg atgatcgaca tcctggtcga gctgatccag agtgtatgct gggccacttg
      421 ctgagaatac tcttcaagaa tgatgatttc atgaatgcac tggtgaatgc atatgtgatg
      481 acaagccgag agcccccttt aaacactgca gcttgcagac tcctattaga catcatgcca
      541 gggctggaaa ctgctgtcgt ctttcaagaa aaggagggaa ttgtcgagaa tcttttcaaa
      601 tgggcccgag aggccgatca accattgagg acatattcta ctggactgtt aggaggtgct
      661 atggaaaatc aagacattgc tgccaactat agagatgaaa attcacagct ggtggcaata
      721 gtgcttcgaa gactgaggga gctacagcta caggaagtgg ctttgcggca ggaaaacaag
      781 cgtcccagtc cacggaagct ctcttctgaa ccccttttgc ctctggatga ggaggctgtg
      841 gatatggact atggtgacat ggctgtagat gtagtggatg gagaccaaga ggaagcttct
      901 ggagacatgg agatctcctt tcatcttgat tcaggccaca agactagtag cagagtgaac
      961 tcaacaacca aacctgagga tggaggatta aagaaaaaca agtcagcaaa acagggtgac
     1021 agagagaact ttaggaaagc caagcaaaag ttgggtttct catcttctga tccagatcgc
     1081 atgtttgttg agctgtctaa tagcagttgg tcagaaatgt ctccctgggt gattggcacc
     1141 aattataccc tttatcctat gactcctgct atcgagcagc gactcattct ccaatatttg
     1201 acccctctag gagaatatca ggagctactt cccatattca tgcaacttgg atcacgggag
     1261 ctgatgatgt tctatattga cctgaagcaa actaatgatg tcctgcttac atttgaggca
     1321 ctaaagcacc tagcatctct cctgctccat aacaaatttg ccacagaatt tgttgcgcat
     1381 ggtggagtac agaaattact ggaaatacct cgtccttcta tggctgcaac cggtgtgtct
     1441 atgtgtttgt attacctatc ctacaatcag gatgccatgg aaagagtttg catgcatccc
     1501 cacaatgttc tgtctgatgt ggtgaactat accctgtggt taatggagtg ttctcatgct
     1561 tcaggatgct gccatgctac catgtttttt tcaatttgct tctcatttcg ggccgtcttg
     1621 gagctctttg accgctatga tggtcttcgt cgtctggtga acttgatcag tactttggag
     1681 attctaaatt tggaagatca gggtgcactt ctgagtgatg atgaaatatt tgctagccgc
     1741 caaactggga aacatacctg catggccttg cgcaaatact ttgaggctca cctggccatt
     1801 aaattggaac aagtgaagca gtcacttcag aggactgagg gtggcattct tgtccaccca
     1861 caacccccgt acaaggcatg ctcatatact catgaacaga ttgtggaaat gatggaattt
     1921 ttgatagaat atggcccagc gcagctatat tgggaaccag ctgaagtttt cctcaaactt
     1981 tcttgtgtgc aactcttgtt gcagcttatt tctattgcct gcaattggaa gacctattat
     2041 gcaaggaatg acactgtgcg ctttgctttg gatgtcctgg ctattcttac tgtggtgcca
     2101 aaaatccagc tccagttggc agaatcagtg gacgtgttgg atgaggctgg atctacagtc
     2161 tctactgtag gtatcagcat tattttggga gtggctgagg gtgagttctt catccatgat
     2221 gctgaaattc agaagtcagc acttcagatt atcatcaatt gtgtgtgtgg cccagataac
     2281 cgaatatcca gtattggtaa atttatctct ggtactcctc ggagaaagct gcctcagaac
     2341 cctaaaagca gtgagcacac cctggccaag atgtggaatg tggttcagtc caacaacggc
     2401 atcaaggtgc tcctgtcctt actgtccatt aagatgccca tcacagatgc agaccaaatc
     2461 cgggccctgg cctgcaaagc cctagtgggc ctgtctcgca gtagcactgt ccggcagatc
     2521 atcagtaaac tgcccctttt cagcagctgc cagatccagc agctgatgaa ggagcctgtg
     2581 ctgcaggaca agcgcagtga ccatgtcaag ttctgcaagt atgctgctga actcattgaa
     2641 cgggtgtcag gaaaaccact tctcattggc actgatgttt ccctagcacg actgcagaaa
     2701 gcagatgttg ttgcccagtc aaggatctcc ttccctgaga aagagctgct tttgttgata
     2761 cgaaaccatc ttatttctaa agggcttgga gaaacagcaa ccgtgctgac aaaagaggct
     2821 gacctgccca tgactgctgc ctcccattct tctgccttta ccccagtcac tgctgctgct
     2881 tctcctgtct ctctaccccg aacccctcgt atcgctaatg gcattgcaac tcgtctgggc
     2941 agccatgctg ctgtgggtgc ctctgcgcct tctgccccta ctgctcatcc tcagccacgg
     3001 cccccccagg gtccgctagc tctgcccggc ccatcttatg caggcaactc ccctttgatt
     3061 ggtagaatca gttttatcag agagaggcca tcaccctgca atggcaggaa aatcagagtg
     3121 ttgcggcaga agtcggacca tggtgcctac agccaaagcc cagccataaa aaaacagctg
     3181 gacagacatc ttccttcccc acctacgctg gacagtataa tcacagagta tcttagagaa
     3241 caacatgctc gctgcaagaa tccagttgcc acctgcccac ctttctccct ctttactcct
     3301 caccaatgtc ctgagccaaa acagaggcgg caagcgccaa taaactttac gtcaaggcta
     3361 aaccgcaggg catcatttcc aaagtatgga ggggtggatg gcggatgctt tgataggcac
     3421 cttatcttta gcagattccg tcctatttca gtgttccggg aagccaatga agatgagagt
     3481 ggcttcacct gctgtgcatt ctcagcacgg gagcggttcc tgatgcttgg cacctgcaca
     3541 gggcagctga agctctataa tgtgtttagt ggacaggagg aggccagcta taactgtcac
     3601 aactcagcca tcacacatct tgaaccttcc agggatgggt ccttgctgct gacatctgct
     3661 acttggagcc agcctttgtc tgcactttgg ggaatgaagt cagtatttga tatgaagcat
     3721 tccttcacag aagatcacta tgttgagttc agtaagcact cccaggatcg ggtcatcggc
     3781 acaaaaggag acattgccca catttatgat attcagactg gcaacaagct gttgactctg
     3841 tttaacccag atcttgccaa caactacaag aggaactgtg ccacctttaa tcctacagat
     3901 gatcttgtct taaatgatgg cgtcctctgg gatgtccgct ctgcacaggc catccacaag
     3961 tttgacaagt tcaatatgaa catcagtggt gttttccatc caaatggact ggaggtgatc
     4021 attaatactg agatttggga ccttcgaact tttcatcttt tgcatactgt tcccgctctg
     4081 gatcagtgtc gcgtggtgtt caatcacacg ggaacagtga tgtatggagc tatgttgcag
     4141 gcagatgatg aagatgactt aatggaagag aggatgaaaa gcccctttgg gtcatccttc
     4201 cgaacattta atgcaactga ctacaaacct atagcaacca ttgatgtgaa acggaacatc
     4261 tttgacctgt gtacagacac caaagactgc tatcttgctg tcattgagaa tcaaggcagc
     4321 atggatgccc tgaacatgga cacagtatgc aggctgtatg aagtgggcag gcagcgtctg
     4381 gcagaggatg aggatgaaga ggaggaccag gaagaggaag aacaggagga agaagatgat
     4441 gatgaagatg atgatgacac cgatgattta gatgagcttg acactgacca gttgctggag
     4501 gcggagttgg aggaggacga caataatgag aacgcagggg aagatgggga caatgacttc
     4561 tctccctctg atgaggagct agcaaacctt ctagaggagg gagaggacgg ggaggatgaa
     4621 gactctgatg cagatgagga ggtggaactg atcctggggg acactgacag ctctgacaac
     4681 tctgatttgg aagatgacat catcttatct ctgaatgagt gaggagccat cactgcttgg
     4741 aagagattct tggcaggcga gaaactgagt caaatgaatt cagaacatat tcccttctct
     4801 ttctcccagg gctgtctgtc ttttaaggag ctgcatgccc tgcattcaga agattatggc
     4861 ttagagagcc tcattggcac ccgagggtcc ttccagaatc aataaccacc acaaaaatga
     4921 caacagggac taggccctac tctgcacccc ccacatccac cccccaccat ttcctaggat
     4981 ggacatatct ttcaagggga aaaaaaaacc atgtctctgg ggtatttcac aatatatttt
     5041 ctttgtatag tgttctttac ttaaagaaca agaaatagtt ttttataaaa ctttaaaaag
     5101 gaaaaaaaaa caggcattta taactgaggg tatgaactta tatccaccag gtctcttgtc
     5161 cctgcacttc attttctttg gaagaaaatg atgtctaaag aacaatatag aggcattttt
     5221 acatacgtat ttaaatgaaa aggaaaatcg tggtttctta aattgataag gattaagaat
     5281 attttattat aaatataata tatgattttt taacctgttt tgttgcctca tatgctgtca
     5341 ggttaatttg ttttccttcg tgccagaggt ggggaggaag gcactctgtc tgctgggtaa
     5401 atgcctaaat tcactcacct tcatggtttg ggggcagcat ggtcattgtg gatattggtt
     5461 ttgtggagtt gagggaactt aggatataag ttcactccct ctatttttct ttgtgattca
     5521 gtttttcaaa aatctttttt tcttcccttt ctccccattg tggaaattac aaatcaaagg
     5581 cctttttctt taatgtaaag tgtatttatt taaaaaaaat acaaaataaa ctacaagtct
     5641 gtctttgtt
//
