LOCUS       NM_001918              10799 bp    mRNA    linear   PRI 13-NOV-2024
DEFINITION  Homo sapiens dihydrolipoamide branched chain transacylase E2 (DBT),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001918
VERSION     NM_001918.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Hwang,R.D., Lu,Y., Tang,Q., Periz,G., Park,G., Li,X., Xiang,Q.,
            Liu,Y., Zhang,T. and Wang,J.
  TITLE     DBT is a metabolic switch for maintenance of proteostasis under
            proteasomal impairment
  JOURNAL   Elife 12, 91002 (2024)
   PUBMED   39255192
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  AUTHORS   Zhang,C., Huang,G., Yang,J., Jiang,Y., Huang,R., Ye,Z., Huang,Y.,
            Hu,H. and Xi,X.
  TITLE     Overexpression of DBT suppresses the aggressiveness of renal clear
            cell carcinoma and correlates with immune infiltration
  JOURNAL   Front Immunol 14, 1197011 (2023)
   PUBMED   37383233
  REMARK    GeneRIF: Overexpression of DBT suppresses the aggressiveness of
            renal clear cell carcinoma and correlates with immune infiltration.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10799)
  AUTHORS   Alijanpour,M., Jazayeri,O., Soleimani Amiri,S. and Brosens,E.
  TITLE     Pathogenic Homozygous Mutations in the DBT Gene (c.1174A>C) Result
            in Maple Syrup Urine Disease in a rs12021720 Carrier
  JOURNAL   Lab Med 53 (6), 596-601 (2022)
   PUBMED   35657820
  REMARK    GeneRIF: Pathogenic Homozygous Mutations in the DBT Gene
            (c.1174A>C) Result in Maple Syrup Urine Disease in a rs12021720
            Carrier.
REFERENCE   4  (bases 1 to 10799)
  AUTHORS   Yang,J., Xiu,J., Sun,Y., Liu,F., Shang,X. and Li,G.
  TITLE     Three novel mutations of the BCKDHA, BCKDHB and DBT genes in
            Chinese children with maple syrup urine disease
  JOURNAL   J Pediatr Endocrinol Metab 35 (3), 303-312 (2021)
   PUBMED   34883003
  REMARK    GeneRIF: Three novel mutations of the BCKDHA, BCKDHB and DBT genes
            in Chinese children with maple syrup urine disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10799)
  AUTHORS   Tsuruta,M., Mitsubuchi,H., Mardy,S., Miura,Y., Hayashida,Y.,
            Kinugasa,A., Ishitsu,T., Matsuda,I. and Indo,Y.
  TITLE     Molecular basis of intermittent maple syrup urine disease: novel
            mutations in the E2 gene of the branched-chain alpha-keto acid
            dehydrogenase complex
  JOURNAL   J Hum Genet 43 (2), 91-100 (1998)
   PUBMED   9621512
REFERENCE   6  (bases 1 to 10799)
  AUTHORS   Strauss,K.A., Puffenberger,E.G. and Carson,V.J.
  TITLE     Maple Syrup Urine Disease
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301495
REFERENCE   7  (bases 1 to 10799)
  AUTHORS   Lau,K.S., Herring,W.J., Chuang,J.L., McKean,M., Danner,D.J.,
            Cox,R.P. and Chuang,D.T.
  TITLE     Structure of the gene encoding dihydrolipoyl transacylase (E2)
            component of human branched chain alpha-keto acid dehydrogenase
            complex and characterization of an E2 pseudogene
  JOURNAL   J Biol Chem 267 (33), 24090-24096 (1992)
   PUBMED   1429740
REFERENCE   8  (bases 1 to 10799)
  AUTHORS   Lau,K.S., Chuang,J.L., Herring,W.J., Danner,D.J., Cox,R.P. and
            Chuang,D.T.
  TITLE     The complete cDNA sequence for dihydrolipoyl transacylase (E2) of
            human branched-chain alpha-keto acid dehydrogenase complex
  JOURNAL   Biochim Biophys Acta 1132 (3), 319-321 (1992)
   PUBMED   1420314
REFERENCE   9  (bases 1 to 10799)
  AUTHORS   Zneimer,S.M., Lau,K.S., Eddy,R.L., Shows,T.B., Chuang,J.L.,
            Chuang,D.T. and Cox,R.P.
  TITLE     Regional assignment of two genes of the human branched-chain
            alpha-keto acid dehydrogenase complex: the E1 beta gene (BCKDHB) to
            chromosome 6p21-22 and the E2 gene (DBT) to chromosome 1p31
  JOURNAL   Genomics 10 (3), 740-747 (1991)
   PUBMED   1889817
REFERENCE   10 (bases 1 to 10799)
  AUTHORS   Fisher,C.W., Lau,K.S., Fisher,C.R., Wynn,R.M., Cox,R.P. and
            Chuang,D.T.
  TITLE     A 17-bp insertion and a Phe215----Cys missense mutation in the
            dihydrolipoyl transacylase (E2) mRNA from a thiamine-responsive
            maple syrup urine disease patient WG-34
  JOURNAL   Biochem Biophys Res Commun 174 (2), 804-809 (1991)
   PUBMED   1847055
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA274670.1, BC016675.1,
            AL445928.8 and AK024946.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 18, 2020 this sequence version replaced NM_001918.4.
            
            Summary: The branched-chain alpha-keto acid dehydrogenase complex
            (BCKD) is an inner-mitochondrial enzyme complex involved in the
            breakdown of the branched-chain amino acids isoleucine, leucine,
            and valine. The BCKD complex is thought to be composed of a core of
            24 transacylase (E2) subunits, and associated decarboxylase (E1),
            dehydrogenase (E3), and regulatory subunits. This gene encodes the
            transacylase (E2) subunit. Mutations in this gene result in maple
            syrup urine disease, type 2. Alternatively spliced transcript
            variants have been described, but their biological validity has not
            been determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X66785.1, SRR1803614.179918.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000370132.8/
                                                  ENSP00000359151.3
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                DA274670.1         20-33
            15-1163             BC016675.1         1-1149
            1164-1164           AL445928.8         101392-101392       c
            1165-2399           BC016675.1         1151-2385
            2400-10017          AL445928.8         82589-90206         c
            10018-10796         AK024946.1         1281-2059
            10797-10799         AL445928.8         81807-81809         c
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.2"
     gene            1..10799
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="dihydrolipoamide branched chain transacylase E2"
                     /db_xref="GeneID:1629"
                     /db_xref="HGNC:HGNC:2698"
                     /db_xref="MIM:248610"
     exon            1..65
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1463
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /EC_number="2.3.1.168"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; branched chain acyltransferase, E2 component;
                     dihydrolipoyl transacylase; dihydrolipoyllysine-residue
                     (2-methylpropanoyl)transferase; lipoamide acyltransferase
                     component of mitochondrial branched-chain alpha-keto acid
                     dehydrogenase complex; BCKAD E2 subunit; mitochondrial
                     branched chain alpha-keto acid dehydrogenase transacylase
                     subunit (E2b); E2 component of branched chain alpha-keto
                     acid dehydrogenase complex; branched chain 2-oxo-acid
                     dehydrogenase complex component E2; 52 kDa mitochondrial
                     autoantigen of primary biliary cirrhosis; lipoamide
                     acyltransferase component of branched-chain alpha-keto
                     acid dehydrogenase complex, mitochondrial; branched-chain
                     alpha-keto acid dehydrogenase complex component E2;
                     dihydrolipoamide acetyltransferase component of
                     branched-chain alpha-keto acid dehydrogenase complex"
                     /codon_start=1
                     /product="lipoamide acyltransferase component of
                     branched-chain alpha-keto acid dehydrogenase complex,
                     mitochondrial isoform 1 precursor"
                     /protein_id="NP_001909.4"
                     /db_xref="CCDS:CCDS767.1"
                     /db_xref="GeneID:1629"
                     /db_xref="HGNC:HGNC:2698"
                     /db_xref="MIM:248610"
                     /translation="MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPS
                     FKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQ
                     SDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDE
                     HTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILP
                     PSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHF
                     GYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQ
                     NITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVSQLSTTDLT
                     GGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSA
                     DHRVIDGATMSRFSNLWKSYLENPAFMLLDLK"
     exon            66..189
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            190..265
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            266..447
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            448..569
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            570..786
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            787..953
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1031
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1223
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1295
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..10799
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3777..3782
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="hexamer: AATAAA"
     polyA_site      3807
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="major polyA site"
     regulatory      4113..4118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="hexamer: AATAAA"
     polyA_site      4131
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
     regulatory      10775..10780
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="hexamer: AATAAA"
     polyA_site      10799
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
ORIGIN      
        1 atttccgggg taagatggct gcagtccgta tgctgagaac ctggagcagg aatgcgggga
       61 agctgatttg tgttcgctat tttcaaacat gtggtaatgt tcatgttttg aagccaaatt
      121 atgtgtgttt ctttggttat ccttcattca agtatagtca tccacatcac ttcctgaaaa
      181 caactgctgc tctccgtgga caggttgttc agttcaagct ctcagacatt ggagaaggga
      241 ttagagaagt aactgttaaa gaatggtatg taaaagaagg agatacagtg tctcagtttg
      301 atagcatctg tgaagttcaa agtgataaag cttctgttac catcactagt cgttatgatg
      361 gagtcattaa aaaactctat tataatctag acgatattgc ctatgtgggg aagccattag
      421 tagacataga aacggaagct ttaaaagatt cagaagaaga tgttgttgaa actcctgcag
      481 tgtctcatga tgaacataca caccaagaga taaagggccg aaaaacactg gcaactcctg
      541 cagttcgccg tctggcaatg gaaaacaata ttaagctgag tgaagttgtt ggctcaggaa
      601 aagatggcag aatacttaaa gaagatatcc tcaactattt ggaaaagcag acaggagcta
      661 tattgcctcc ttcacccaaa gttgaaatta tgccacctcc accaaagcca aaagacatga
      721 ctgttcctat actagtatca aaacctccgg tattcacagg caaagacaaa acagaaccca
      781 taaaaggctt tcaaaaagca atggtcaaga ctatgtctgc agccctgaag atacctcatt
      841 ttggttattg tgatgagatt gaccttactg aactggttaa gctccgagaa gaattaaaac
      901 ccattgcatt tgctcgtgga attaaactct cctttatgcc tttcttctta aaggctgctt
      961 ccttgggatt actacagttt cctatcctta acgcttctgt ggatgaaaac tgccagaata
     1021 taacatataa ggcttctcat aacattggga tagcaatgga tactgagcag ggtttgattg
     1081 tccctaatgt gaaaaatgtt cagatctgct ctatatttga catcgccact gaactgaacc
     1141 gcctccagaa attgggctct gtgagtcagc tcagcaccac tgatcttaca ggaggaacat
     1201 ttactctttc caacattgga tcaattggtg gtacctttgc caaaccagtg ataatgccac
     1261 ctgaagtagc cattggggcc cttggatcaa ttaaggccat tccccgattt aaccagaaag
     1321 gagaagtata taaggcacag ataatgaatg tgagctggtc agctgatcac agagttattg
     1381 atggtgctac aatgtcacgc ttctccaatt tgtggaaatc ctatttagaa aacccagctt
     1441 ttatgctact agatctgaaa tgaagactga taagacattc ttgaactttt tgagcttcca
     1501 aagagtatgt aaaccctagc tgtgccagca catgttcatc tttacaattt atattgtaaa
     1561 tgatttgtat cgtatgatta aggatctaag gcacaatatt tgtcactgtt ctattagact
     1621 ttttactgaa aatgaataat ggtgtaatgg ttctcctggg gctgtcacat tttataggtc
     1681 agagtgtgac ttcttaatat ggtgctgatg tttttgtgtc aatggcttga aactggcaag
     1741 attaacaaaa ttaggccggg catggtggct cacgcctgta atcccagcac tttgggaggc
     1801 ccaggtgggc ggatcacctg aggttagaag tttgagacca gcctggccaa catggtgaaa
     1861 cctggcctct actaaaaata caaaaattag ccgggtgtgg tggtgggtac ctataatccc
     1921 agctacttgg gaggctgagg caggagaatc gcttgaacct gggaggtgga ggttgcagtg
     1981 agctgagatc gtgctattgc actccagcct gggcgacaga gcaagacgcc atctcaaaaa
     2041 caaaaaaaca aaattcatgt tactaaaaga caggtagcca tatacagaca gtatatgccc
     2101 tatttttttt aactgactct taatgaaact ttaattttac ttaattaaga aatggaattt
     2161 atatacaaaa atattttcca tttccgttat tatgctaatt gttgtatgaa ataagtgcaa
     2221 ttatacttct cttttgagat atccaagagt atattcttgc tctgtataga gaatatcatc
     2281 tgatagtgtc ttatttatat taattaatgt ctttgaaaag ggaaaagtat aaactggcct
     2341 taaaattgtc caattatagt tttataacca gtctattaaa ggtgtttgtt taaaatggat
     2401 atagttttag atttgtggta atgctttggt attttcttgg ggaagacctt cacctttgca
     2461 aacttccctc atgtaaggaa ggtactttaa atgtagcagc cactgacatt tcttttttta
     2521 aaaaaaattt gagaagtcta cttcctttta acttttttgg tcttcagcta aaaaatagga
     2581 taagaaatta aggtctattc cattctccat atcctgggta agaatgtaaa taagaggaga
     2641 aggaagagtc taatagtaat tatggatata aaaaataaga aattttgtat agaaatgaag
     2701 gtttcataat gatcattttg ttaaaggtct actttaatca gaaatagcaa cgagatgaat
     2761 gtatccaaca tttcaatttg cattcggaaa tccatgttgt ttctaatatt gtccagttga
     2821 aaactgtatg ccaaaattag ttgtttaagt gaagttttgt gacagaaaaa aggttgtttt
     2881 aatatctact tggtttttct caaaatggaa ataattttaa aatcaggaaa gaataaatca
     2941 gccaggtgtg atgacttgta actgtaatcc cagttatagg ggaggctgaa gcaggaggat
     3001 cacttgaggc caggagtttg agaccagcct gggcaacata gtgagatccc atctcaaaaa
     3061 acattatttt taaaattagc ctggtggctc acgcctgtaa tcccagcact ttgggaggcc
     3121 gaggtggcca gatcacctga ggtcaggagt tcgagaccac cctggccaac atggtgaaac
     3181 cccatctcta cagttttgta aaaatacaaa aaattacctg ggcctggtgg cacacgcctg
     3241 tagtcccagc tacttgggag gctgaggcag gagaattgct tgagcccaag aggtggaggt
     3301 tacagtgagc agagatcaca ccactgcact ccagcctggg tggcagagca acactttgtc
     3361 tcagaaaaaa aaaaaaaaac caaaaagcca agtgtggtgg tgtgcaccta tagtcccagc
     3421 tactcaggaa gctgagacaa gaggatcaat tgagcccagg agttcaaagc tgtagtgagc
     3481 tgtcattgtg ccactatcct ccagtatggg tgacagagtg agacctggtc tctaaaaaat
     3541 aaaataaaat aaaaaaaaca ggtcaaataa atgctgttgt tgtaaaattt cagataatac
     3601 aaagagttaa ccaataaaag aaaaagtcat tcataatctt accactatta acattttgat
     3661 gtatctatct gtatgtatgg ctattctttt ttgttaaaac atgatcatag catatctact
     3721 attttattat ttgattttta aaatttaaca ttatattatg ggtaacctta catgtcaata
     3781 aacaattcca cattgtcatg ctttaaatgg ctgcataggc tgggtgtggt ggctcacgcc
     3841 tgtaatccta ccactttggg aggccaaggc aggtagatca caaggtcagg agttcgagac
     3901 cagcctggcc aatatggtga aacctcatct ctattgaaaa tacaaaaatc agccaggcac
     3961 ggtggcaggc gcctgtagtc cacctactcg ggaggctgag gcaggagaac tgcttgaacc
     4021 cagcggcaga ggttgcagtg agccaagatt gagccactgc actccagcct ggtgatagag
     4081 cgagactctg tctctaaata aataaataaa taaataaatg tctgcatagt attccattgt
     4141 atgcatttat tatacattca aacattaaac atctaccata ccaatcatgg ttctaagcat
     4201 acagtagcca ccttcatgct tccttgattg ggcttacagt ctagtaagga gacagatatt
     4261 aaataactgc ataaaacact gttcttggta ggtaataacc cagatgctat gagccatcca
     4321 gtaaaggtta ccgatcttat ctggaaggat cagaagatgg atgtcccata atttaaccaa
     4381 ttatctattg ataggcattt attgagtttt aaatgaataa tgacatgatc aataaaaatg
     4441 tcctcctcac ttaaagaata gaaaaaccag gagaagggag ttctgaaata agtaagtatg
     4501 taggtccagc atcaatggct caagatactc tattcccaaa taacccttaa gttatctggt
     4561 tctattccaa cccttgggta ttactatctt ctctttggtc tcatgcattg agaattcagc
     4621 aaaggatttg ttccctacag tgtgcacggc ccttaggata gcaggattcc ctgaagaggg
     4681 ccaaaggggc aggagcgcag ccttagtggc agtgctggtc catgaggctc tacaaagcaa
     4741 gggatgggtg tttgggagga ggaaaaggct cttgctgtac tcctagaggt tggaattaaa
     4801 gtggaaaagc cagaaaattc aagtccttta ctaacatgtc tggagattag aaggtatatg
     4861 tacatggggg agaagccaaa attattgctg cgagtggtca gcaagaaaat caaggctctt
     4921 ttcattattt tgagcattcc tctgatattt gaaaaggaag tacaacagga aaggaagtct
     4981 gaggatggaa gctaaaattg gtatgaattt atattttaga gatcaaaatg taccttatgt
     5041 tgaaacctat gtaagaagtg attatgtaga aagagtgaaa gtgatagctc ttagtctgga
     5101 aagcccactg gcttgtttgg gcatttctca tggcttccca ctcaaagtgg atccccaaaa
     5161 tcacttgatg gatttccttg ctgatttcta agtaaactat ggtttaagaa agaaatgaca
     5221 gggctcagca ctgccctaca gtaccaagaa tacaaatgtt tccatgaagt cttcaaaggc
     5281 atttgtaaaa ttcaggctgt aagtgattag ttagttcatt ctgcacttat ttattaactg
     5341 tatattcagt tccaggctct agggtagaga ttatggataa aggtgaatta gatagatgaa
     5401 gtttttgccc tcacagcaaa agctttagcc aataattaaa gctatcactg gaagtgggtc
     5461 tgtgccaata acctagagaa gagcagtgct tttagagttg agctatattc ccaatcagtt
     5521 cttaatggtg gttttacccc cttccctcta cactgtcttt tcttgagatt ggatcatgtg
     5581 tgtgaaccca catcatttcc agtcaaaatc accaagagag gccggacaca gtggctcatg
     5641 cctataatcc cagcactttg ggaggcctaa atgggtgggc ctcttgagtc cagagtttga
     5701 gaccagcctg ggcgacccca gctctaccaa aaaaaccaaa aaacaaaaaa taccaaaaaa
     5761 aaaaaaaatt aggccgatga agtgacatgc acctgtgtgc cacctactca ggaggctgag
     5821 gtggcaggat cacttgcatc tgggggcaaa ggctgccgtg agctgagatt gtgccattgc
     5881 gctccagcct gggcgacaga gcaagactct gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     5941 gtgtgtgtgt gtgtgtgtgt gtgtgtgtat acacacacac atatatatac acacaccccc
     6001 aggagacctt ttgtctttct tggttcatgt gcccatcagt gtgggcagga gaatagaata
     6061 ctctgtccac acttgagtct tagtcacctt tggagcctag gagtcagcca tcctcattca
     6121 actacatgga tcgagaatgg gaatctggga gaattttttt atgggcagat taaaaaggaa
     6181 gttaactatg taaagtaagg cattgccttc ccaccccatc ccacctcagt tcgtgttaaa
     6241 ttatgaattt ttctaagaga gttctttaat gacatggata gagaggaagc tgaggtggca
     6301 gtagagtagg gagtggaagg caaatacata agatactatt aaactgtgaa gctgtttgtt
     6361 acaattccaa gattttataa tttctccaac aaaaaacatt tgtattttct tcccagttta
     6421 agttcaatgt taatttgaga aataattcta ctctcaaact tcatgttatg ctgacagttc
     6481 aactgatgct gtcaaaatgt gaattactaa cttcagtatt ctctattcat ctgatttttc
     6541 ttaaaatgtt tgcgtgttaa tatactcact agctttaatg ctctactctt atactggcac
     6601 cttaattatg gtaaaaggta aaaggttaag ttaaaattcc caatcctagt tatttggtca
     6661 aacagaagat taacagaact ccatggctta ccacaggaat atttatcaag agaaaaagaa
     6721 gatacctatt tgaatttaca tgacactttc tgtgaaatat ctggattacc agattagtct
     6781 gaaacaaatg ccttccaaat tctacagcgc atgcccaagg cactctttac agtacctgtt
     6841 cgggtctttt cttctaattt taagcaccta cccaaacgtc ttcccaggct tgtcataata
     6901 agcaaataac ctcagagcag acaaagtgat tcaatctaag aaccttgaat ttctctcctt
     6961 ttcacctcaa aatttctatt ctcacacttt ctttttgttt ctctttccga gaagtggttc
     7021 tccatcagca aataccaact gttttctgtc ctctattatt tattccatct cttcctcctg
     7081 atccctgccc catgttgaac ccccattgcc ccactttgat ccttcagtct cttcctcaca
     7141 tccagaactg taccagattt actcccttag cacactgatt aaaatttatt tccatttcct
     7201 tatggaggag gtattagaga ctgatcccag acctctacaa atttcattgt ggcagggtaa
     7261 tgttatgtgt ccataaacct tgttcctttt ctttcagcct gcagttaggt agagccatgt
     7321 catttatttg taactattag aatatgggta tatgggttga agtaatgtag gccatttcca
     7381 gacatggccc ataacacttc tcaagagatt cttcatattc tatctcttcc cctccaacat
     7441 gcacccttca aggttgaaga tggcaccaca caagatagaa ggagccagtg ttccaaatga
     7501 ctaggcagag cagaatgctc ccctccctac accctagcac caattggttt ggatgtgata
     7561 gttgaataat acacttttat tgttttaaga cattaatatt ttgagattaa tctgcaagtc
     7621 tagttagtaa aactcgaact ctgagctgtc taatgcagta gcctctagcc acatgtagct
     7681 atttaaattt aaattttaat tttaattaat caaaattcaa ctcagatata ctagccacat
     7741 ttcaattgct cagtagccac actaattaaa tttaaattta aatagctaca tgtggcttat
     7801 agcaccatac cagatagcac agatgtagac tgtttccatc atcacagaaa attctattgg
     7861 atagtgctgc cttaactaat aagacacaaa tgttcttatt ttttaaatag tccctaaaac
     7921 aaacgaaaaa caaagaactc ccttctctaa tggcaaattt cagtttgcct aaaagaagac
     7981 tcagaatgct tttcttatat tgttacctct gactgccact aaatgaataa atttcctgtc
     8041 ttgctccttc tgccaaactt cttgaatggg tgatctttaa ctccttgcaa tcctggcttt
     8101 tttccccagt gctcacctgt aatcagtgtt ggccacaact cccacccaga tgtctcctaa
     8161 atagttctct gaacccacct ctgtcctgat ctacagacat tcattccaat gctttagcat
     8221 ttcaaggtca tcaaatgact cagtgacatc tcagacagtc agacagacca ggtcaactat
     8281 gaactattta tgccaaacct tacatggttc atgttatcct tgctatccca tcaaaataac
     8341 cacaggcttt tttttttttt ttttttaatt tttgagatgg agtctcactc tgtcaccccg
     8401 actagagtgc agtggtgcga tctcggctct gctcactgca acctccgtct cctgggttca
     8461 aacagtgctc attcctcaac tcccaagtag ctgagattac aggcatgcac catcacgcct
     8521 ggctaatttt tctattttta gcagagatgg ggtttcgcca tgttggccag gctggtctcg
     8581 aactcctgac ctcaagtgat ctgcccgcct ccacctcaca aagctttggg attacaggca
     8641 tgagccacgg tgcctggcct gcagcctcct ttattttaca aaaggcatta ccacttaaac
     8701 actgaagata gcacgaatct ctgatgtttg caagttcaga ataaaatgga aatgaaaatt
     8761 cattatgtgg cagtctcagt actgagcaca aaggctttta accacaagca aattactgtg
     8821 ctagccagac cgtgtgagag aactccaggc cagcttgcta aaactgagca ggagaatttg
     8881 gagagagtcc agaggagagc cacagaaaca actgaagcat gacaagaata attgcagttc
     8941 tgttatagag aggtcatcta tatcaggctt ttatggagta ccatgtggaa tgttaccacg
     9001 cagagcatgg ggaacagctg ttgtgactca acaagagaca ggacagaaga aatgagaaca
     9061 ggtggaagat caataattta ggtttgatat ggggaaagat ggaacagtca aagggattca
     9121 cactgatttt tggctgagca gaaggcagag tctcctccaa cgggcaatgt aagtgccccc
     9181 actcatctac accccgagaa gagccatcaa actgtattaa acttggaaaa agctatttaa
     9241 cttcaagtgt gctgcgagaa aacttcataa tagttcctaa gatgtgctaa aaagtaaagt
     9301 ccaaaaagat cataaagtct gtagagaagt tctaagagtg cagtcagcta taaaaaccta
     9361 gcaatttaat ttcttagaaa aatgtagctg gagttcaaac tgtagtaaca aaggcaagta
     9421 aattaagttg tgggcaggtg taattaagtt aataggaatg gcagggatga atataaatca
     9481 gaacaggact aacagtttga aacattagat attcaaatgc tcagagctga tgagccgagc
     9541 cactacgcta acaactctgc aaaccaaagg tttcacaaat tgcacaagaa cctggcctcg
     9601 atcccattgt gttgctggca taagattaga agggcctgtt ggttcaattg ttgctttgga
     9661 cagaggagga caaacttgcc acagtgaaga aatcattttt aaaaaccacc actgtccttt
     9721 agtgcactaa tttctctaat aactaggtgt atattagaaa taatgatata taagtgcagt
     9781 atactccagt ttcaacagaa tggtgtcact tctgtaaaat gttgattttc agattgtcat
     9841 actttctaaa tacaaaaatt tgtcacagct tccacattta caagatatta aacattccat
     9901 aaaatatttg tagaaatatg aaaaaccaat ttttgtagtt gccttttgaa atgtatacaa
     9961 aatatcccac atgggcaaca tggtaaaacc tcgtctctac aaaaaaaaaa aaaaaaatac
    10021 aaaaagttag ccaggcctgg tggtgcacgc ctgtggtccc agctactcgg gaagctgaag
    10081 tgagatcacc tgtgcccagg aggtcgaagc tgcagtgagc tgtgattgca ccactgcact
    10141 ccagcctggg tgacagagtg agactttgtc tcaaataaac aaaaagagac atatacaaaa
    10201 tatattagaa agttgggtta atatcctaca aacaaagtat tacatagttt gcacaggatt
    10261 atgtgtgtaa aaagttttaa attcagtata gcttttattt ctgcagaact aaaagtagtg
    10321 cataaaaata aaagtgggat gagcatatct ttttaatttg ctatgcagta aaaagtatct
    10381 cagtcaacat ataggaaacc agtgccattt ctgtatcatt tgacaccgaa ggggtattgt
    10441 tttttgtttt gttttgtttt tcttttgaag aaagactttt atgtctttta ttctattctt
    10501 ccgatttggg gtttaaatta cccccaataa gtttgcagaa tattctgatt actactgctt
    10561 aggtgatata tttcatatga gaactatctt tacacaatgg gaaaaaatat ggtaaccact
    10621 tcattcatca gagaagttgt gttttgttta cttgtatttt gtttgctaaa tgaaattggt
    10681 tatttagact catggcatgc tttttctcaa atggagattt aaaagtcatt tccaatgtcc
    10741 ttctaacttc tggccaattc ccagtaggct atagaataaa cagttttcat ataatagtc
//
