LOCUS       NM_001399969           10986 bp    mRNA    linear   PRI 13-NOV-2024
DEFINITION  Homo sapiens dihydrolipoamide branched chain transacylase E2 (DBT),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001399969 XM_005270545
VERSION     NM_001399969.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Hwang,R.D., Lu,Y., Tang,Q., Periz,G., Park,G., Li,X., Xiang,Q.,
            Liu,Y., Zhang,T. and Wang,J.
  TITLE     DBT is a metabolic switch for maintenance of proteostasis under
            proteasomal impairment
  JOURNAL   Elife 12, 91002 (2024)
   PUBMED   39255192
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Zhang,C., Huang,G., Yang,J., Jiang,Y., Huang,R., Ye,Z., Huang,Y.,
            Hu,H. and Xi,X.
  TITLE     Overexpression of DBT suppresses the aggressiveness of renal clear
            cell carcinoma and correlates with immune infiltration
  JOURNAL   Front Immunol 14, 1197011 (2023)
   PUBMED   37383233
  REMARK    GeneRIF: Overexpression of DBT suppresses the aggressiveness of
            renal clear cell carcinoma and correlates with immune infiltration.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10986)
  AUTHORS   Alijanpour,M., Jazayeri,O., Soleimani Amiri,S. and Brosens,E.
  TITLE     Pathogenic Homozygous Mutations in the DBT Gene (c.1174A>C) Result
            in Maple Syrup Urine Disease in a rs12021720 Carrier
  JOURNAL   Lab Med 53 (6), 596-601 (2022)
   PUBMED   35657820
  REMARK    GeneRIF: Pathogenic Homozygous Mutations in the DBT Gene
            (c.1174A>C) Result in Maple Syrup Urine Disease in a rs12021720
            Carrier.
REFERENCE   4  (bases 1 to 10986)
  AUTHORS   Yang,J., Xiu,J., Sun,Y., Liu,F., Shang,X. and Li,G.
  TITLE     Three novel mutations of the BCKDHA, BCKDHB and DBT genes in
            Chinese children with maple syrup urine disease
  JOURNAL   J Pediatr Endocrinol Metab 35 (3), 303-312 (2021)
   PUBMED   34883003
  REMARK    GeneRIF: Three novel mutations of the BCKDHA, BCKDHB and DBT genes
            in Chinese children with maple syrup urine disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10986)
  AUTHORS   Tsuruta,M., Mitsubuchi,H., Mardy,S., Miura,Y., Hayashida,Y.,
            Kinugasa,A., Ishitsu,T., Matsuda,I. and Indo,Y.
  TITLE     Molecular basis of intermittent maple syrup urine disease: novel
            mutations in the E2 gene of the branched-chain alpha-keto acid
            dehydrogenase complex
  JOURNAL   J Hum Genet 43 (2), 91-100 (1998)
   PUBMED   9621512
REFERENCE   6  (bases 1 to 10986)
  AUTHORS   Strauss,K.A., Puffenberger,E.G. and Carson,V.J.
  TITLE     Maple Syrup Urine Disease
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301495
REFERENCE   7  (bases 1 to 10986)
  AUTHORS   Lau,K.S., Herring,W.J., Chuang,J.L., McKean,M., Danner,D.J.,
            Cox,R.P. and Chuang,D.T.
  TITLE     Structure of the gene encoding dihydrolipoyl transacylase (E2)
            component of human branched chain alpha-keto acid dehydrogenase
            complex and characterization of an E2 pseudogene
  JOURNAL   J Biol Chem 267 (33), 24090-24096 (1992)
   PUBMED   1429740
REFERENCE   8  (bases 1 to 10986)
  AUTHORS   Lau,K.S., Chuang,J.L., Herring,W.J., Danner,D.J., Cox,R.P. and
            Chuang,D.T.
  TITLE     The complete cDNA sequence for dihydrolipoyl transacylase (E2) of
            human branched-chain alpha-keto acid dehydrogenase complex
  JOURNAL   Biochim Biophys Acta 1132 (3), 319-321 (1992)
   PUBMED   1420314
REFERENCE   9  (bases 1 to 10986)
  AUTHORS   Zneimer,S.M., Lau,K.S., Eddy,R.L., Shows,T.B., Chuang,J.L.,
            Chuang,D.T. and Cox,R.P.
  TITLE     Regional assignment of two genes of the human branched-chain
            alpha-keto acid dehydrogenase complex: the E1 beta gene (BCKDHB) to
            chromosome 6p21-22 and the E2 gene (DBT) to chromosome 1p31
  JOURNAL   Genomics 10 (3), 740-747 (1991)
   PUBMED   1889817
REFERENCE   10 (bases 1 to 10986)
  AUTHORS   Fisher,C.W., Lau,K.S., Fisher,C.R., Wynn,R.M., Cox,R.P. and
            Chuang,D.T.
  TITLE     A 17-bp insertion and a Phe215----Cys missense mutation in the
            dihydrolipoyl transacylase (E2) mRNA from a thiamine-responsive
            maple syrup urine disease patient WG-34
  JOURNAL   Biochem Biophys Res Commun 174 (2), 804-809 (1991)
   PUBMED   1847055
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL445928.8.
            
            On Jan 18, 2022 this sequence version replaced XM_005270545.4.
            
            Summary: The branched-chain alpha-keto acid dehydrogenase complex
            (BCKD) is an inner-mitochondrial enzyme complex involved in the
            breakdown of the branched-chain amino acids isoleucine, leucine,
            and valine. The BCKD complex is thought to be composed of a core of
            24 transacylase (E2) subunits, and associated decarboxylase (E1),
            dehydrogenase (E3), and regulatory subunits. This gene encodes the
            transacylase (E2) subunit. Mutations in this gene result in maple
            syrup urine disease, type 2. Alternatively spliced transcript
            variants have been described, but their biological validity has not
            been determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.156511.1,
                                           SRR1803616.156754.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AL445928.8         144658-144722       c
            66-252              AL445928.8         143787-143973       c
            253-376             AL445928.8         135649-135772       c
            377-452             AL445928.8         130324-130399       c
            453-634             AL445928.8         125621-125802       c
            635-756             AL445928.8         113514-113635       c
            757-973             AL445928.8         110871-111087       c
            974-1140            AL445928.8         109705-109871       c
            1141-1218           AL445928.8         105582-105659       c
            1219-1410           AL445928.8         101333-101524       c
            1411-1482           AL445928.8         101118-101189       c
            1483-10986          AL445928.8         81807-91310         c
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.2"
     gene            1..10986
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="dihydrolipoamide branched chain transacylase E2"
                     /db_xref="GeneID:1629"
                     /db_xref="HGNC:HGNC:2698"
                     /db_xref="MIM:248610"
     exon            1..65
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            66..252
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            253..376
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            377..452
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            453..634
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            635..756
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     CDS             745..1650
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /EC_number="2.3.1.168"
                     /note="isoform 2 is encoded by transcript variant 2;
                     branched chain acyltransferase, E2 component;
                     dihydrolipoyl transacylase; dihydrolipoyllysine-residue
                     (2-methylpropanoyl)transferase; lipoamide acyltransferase
                     component of mitochondrial branched-chain alpha-keto acid
                     dehydrogenase complex; BCKAD E2 subunit; mitochondrial
                     branched chain alpha-keto acid dehydrogenase transacylase
                     subunit (E2b); E2 component of branched chain alpha-keto
                     acid dehydrogenase complex; branched chain 2-oxo-acid
                     dehydrogenase complex component E2; 52 kDa mitochondrial
                     autoantigen of primary biliary cirrhosis; lipoamide
                     acyltransferase component of branched-chain alpha-keto
                     acid dehydrogenase complex, mitochondrial; branched-chain
                     alpha-keto acid dehydrogenase complex component E2;
                     dihydrolipoamide acetyltransferase component of
                     branched-chain alpha-keto acid dehydrogenase complex"
                     /codon_start=1
                     /product="lipoamide acyltransferase component of
                     branched-chain alpha-keto acid dehydrogenase complex,
                     mitochondrial isoform 2"
                     /protein_id="NP_001386898.1"
                     /db_xref="CCDS:CCDS91012.1"
                     /db_xref="GeneID:1629"
                     /db_xref="HGNC:HGNC:2698"
                     /db_xref="MIM:248610"
                     /translation="MENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEI
                     MPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEID
                     LTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKAS
                     HNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVSQLSTTDLTGGTFTLS
                     NIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDG
                     ATMSRFSNLWKSYLENPAFMLLDLK"
     exon            757..973
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1140
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1218
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1410
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1482
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..10986
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3964..3969
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="hexamer: AATAAA"
     polyA_site      3994
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="major polyA site"
     regulatory      4300..4305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="hexamer: AATAAA"
     polyA_site      4318
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
     regulatory      10962..10967
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
                     /note="hexamer: AATAAA"
     polyA_site      10986
                     /gene="DBT"
                     /gene_synonym="BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2;
                     BCOADC-E2; E2; E2B"
ORIGIN      
        1 atttccgggg taagatggct gcagtccgta tgctgagaac ctggagcagg aatgcgggga
       61 agctgatgct cctgccttgg tgaccccgat cttacaaaaa ctttcaagag cctgctatgt
      121 accagtgctg tgttaggcac tggagataca aatatgaaat agacatcata tcttccttct
      181 caagagatta caaccttgtg gtggaggaca aaggagaggg agacagatag aaattacaac
      241 ccagcatgag agatttgtgt tcgctatttt caaacatgtg gtaatgttca tgttttgaag
      301 ccaaattatg tgtgtttctt tggttatcct tcattcaagt atagtcatcc acatcacttc
      361 ctgaaaacaa ctgctgctct ccgtggacag gttgttcagt tcaagctctc agacattgga
      421 gaagggatta gagaagtaac tgttaaagaa tggtatgtaa aagaaggaga tacagtgtct
      481 cagtttgata gcatctgtga agttcaaagt gataaagctt ctgttaccat cactagtcgt
      541 tatgatggag tcattaaaaa actctattat aatctagacg atattgccta tgtggggaag
      601 ccattagtag acatagaaac ggaagcttta aaagattcag aagaagatgt tgttgaaact
      661 cctgcagtgt ctcatgatga acatacacac caagagataa agggccgaaa aacactggca
      721 actcctgcag ttcgccgtct ggcaatggaa aacaatatta agctgagtga agttgttggc
      781 tcaggaaaag atggcagaat acttaaagaa gatatcctca actatttgga aaagcagaca
      841 ggagctatat tgcctccttc acccaaagtt gaaattatgc cacctccacc aaagccaaaa
      901 gacatgactg ttcctatact agtatcaaaa cctccggtat tcacaggcaa agacaaaaca
      961 gaacccataa aaggctttca aaaagcaatg gtcaagacta tgtctgcagc cctgaagata
     1021 cctcattttg gttattgtga tgagattgac cttactgaac tggttaagct ccgagaagaa
     1081 ttaaaaccca ttgcatttgc tcgtggaatt aaactctcct ttatgccttt cttcttaaag
     1141 gctgcttcct tgggattact acagtttcct atccttaacg cttctgtgga tgaaaactgc
     1201 cagaatataa catataaggc ttctcataac attgggatag caatggatac tgagcagggt
     1261 ttgattgtcc ctaatgtgaa aaatgttcag atctgctcta tatttgacat cgccactgaa
     1321 ctgaaccgcc tccagaaatt gggctctgtg agtcagctca gcaccactga tcttacagga
     1381 ggaacattta ctctttccaa cattggatca attggtggta cctttgccaa accagtgata
     1441 atgccacctg aagtagccat tggggccctt ggatcaatta aggccattcc ccgatttaac
     1501 cagaaaggag aagtatataa ggcacagata atgaatgtga gctggtcagc tgatcacaga
     1561 gttattgatg gtgctacaat gtcacgcttc tccaatttgt ggaaatccta tttagaaaac
     1621 ccagctttta tgctactaga tctgaaatga agactgataa gacattcttg aactttttga
     1681 gcttccaaag agtatgtaaa ccctagctgt gccagcacat gttcatcttt acaatttata
     1741 ttgtaaatga tttgtatcgt atgattaagg atctaaggca caatatttgt cactgttcta
     1801 ttagactttt tactgaaaat gaataatggt gtaatggttc tcctggggct gtcacatttt
     1861 ataggtcaga gtgtgacttc ttaatatggt gctgatgttt ttgtgtcaat ggcttgaaac
     1921 tggcaagatt aacaaaatta ggccgggcat ggtggctcac gcctgtaatc ccagcacttt
     1981 gggaggccca ggtgggcgga tcacctgagg ttagaagttt gagaccagcc tggccaacat
     2041 ggtgaaacct ggcctctact aaaaatacaa aaattagccg ggtgtggtgg tgggtaccta
     2101 taatcccagc tacttgggag gctgaggcag gagaatcgct tgaacctggg aggtggaggt
     2161 tgcagtgagc tgagatcgtg ctattgcact ccagcctggg cgacagagca agacgccatc
     2221 tcaaaaacaa aaaaacaaaa ttcatgttac taaaagacag gtagccatat acagacagta
     2281 tatgccctat tttttttaac tgactcttaa tgaaacttta attttactta attaagaaat
     2341 ggaatttata tacaaaaata ttttccattt ccgttattat gctaattgtt gtatgaaata
     2401 agtgcaatta tacttctctt ttgagatatc caagagtata ttcttgctct gtatagagaa
     2461 tatcatctga tagtgtctta tttatattaa ttaatgtctt tgaaaaggga aaagtataaa
     2521 ctggccttaa aattgtccaa ttatagtttt ataaccagtc tattaaaggt gtttgtttaa
     2581 aatggatata gttttagatt tgtggtaatg ctttggtatt ttcttgggga agaccttcac
     2641 ctttgcaaac ttccctcatg taaggaaggt actttaaatg tagcagccac tgacatttct
     2701 ttttttaaaa aaaatttgag aagtctactt ccttttaact tttttggtct tcagctaaaa
     2761 aataggataa gaaattaagg tctattccat tctccatatc ctgggtaaga atgtaaataa
     2821 gaggagaagg aagagtctaa tagtaattat ggatataaaa aataagaaat tttgtataga
     2881 aatgaaggtt tcataatgat cattttgtta aaggtctact ttaatcagaa atagcaacga
     2941 gatgaatgta tccaacattt caatttgcat tcggaaatcc atgttgtttc taatattgtc
     3001 cagttgaaaa ctgtatgcca aaattagttg tttaagtgaa gttttgtgac agaaaaaagg
     3061 ttgttttaat atctacttgg tttttctcaa aatggaaata attttaaaat caggaaagaa
     3121 taaatcagcc aggtgtgatg acttgtaact gtaatcccag ttatagggga ggctgaagca
     3181 ggaggatcac ttgaggccag gagtttgaga ccagcctggg caacatagtg agatcccatc
     3241 tcaaaaaaca ttatttttaa aattagcctg gtggctcacg cctgtaatcc cagcactttg
     3301 ggaggccgag gtggccagat cacctgaggt caggagttcg agaccaccct ggccaacatg
     3361 gtgaaacccc atctctacag ttttgtaaaa atacaaaaaa ttacctgggc ctggtggcac
     3421 acgcctgtag tcccagctac ttgggaggct gaggcaggag aattgcttga gcccaagagg
     3481 tggaggttac agtgagcaga gatcacacca ctgcactcca gcctgggtgg cagagcaaca
     3541 ctttgtctca gaaaaaaaaa aaaaaaccaa aaagccaagt gtggtggtgt gcacctatag
     3601 tcccagctac tcaggaagct gagacaagag gatcaattga gcccaggagt tcaaagctgt
     3661 agtgagctgt cattgtgcca ctatcctcca gtatgggtga cagagtgaga cctggtctct
     3721 aaaaaataaa ataaaataaa aaaaacaggt caaataaatg ctgttgttgt aaaatttcag
     3781 ataatacaaa gagttaacca ataaaagaaa aagtcattca taatcttacc actattaaca
     3841 ttttgatgta tctatctgta tgtatggcta ttcttttttg ttaaaacatg atcatagcat
     3901 atctactatt ttattatttg atttttaaaa tttaacatta tattatgggt aaccttacat
     3961 gtcaataaac aattccacat tgtcatgctt taaatggctg cataggctgg gtgtggtggc
     4021 tcacgcctgt aatcctacca ctttgggagg ccaaggcagg tagatcacaa ggtcaggagt
     4081 tcgagaccag cctggccaat atggtgaaac ctcatctcta ttgaaaatac aaaaatcagc
     4141 caggcacggt ggcaggcgcc tgtagtccac ctactcggga ggctgaggca ggagaactgc
     4201 ttgaacccag cggcagaggt tgcagtgagc caagattgag ccactgcact ccagcctggt
     4261 gatagagcga gactctgtct ctaaataaat aaataaataa ataaatgtct gcatagtatt
     4321 ccattgtatg catttattat acattcaaac attaaacatc taccatacca atcatggttc
     4381 taagcataca gtagccacct tcatgcttcc ttgattgggc ttacagtcta gtaaggagac
     4441 agatattaaa taactgcata aaacactgtt cttggtaggt aataacccag atgctatgag
     4501 ccatccagta aaggttaccg atcttatctg gaaggatcag aagatggatg tcccataatt
     4561 taaccaatta tctattgata ggcatttatt gagttttaaa tgaataatga catgatcaat
     4621 aaaaatgtcc tcctcactta aagaatagaa aaaccaggag aagggagttc tgaaataagt
     4681 aagtatgtag gtccagcatc aatggctcaa gatactctat tcccaaataa cccttaagtt
     4741 atctggttct attccaaccc ttgggtatta ctatcttctc tttggtctca tgcattgaga
     4801 attcagcaaa ggatttgttc cctacagtgt gcacggccct taggatagca ggattccctg
     4861 aagagggcca aaggggcagg agcgcagcct tagtggcagt gctggtccat gaggctctac
     4921 aaagcaaggg atgggtgttt gggaggagga aaaggctctt gctgtactcc tagaggttgg
     4981 aattaaagtg gaaaagccag aaaattcaag tcctttacta acatgtctgg agattagaag
     5041 gtatatgtac atgggggaga agccaaaatt attgctgcga gtggtcagca agaaaatcaa
     5101 ggctcttttc attattttga gcattcctct gatatttgaa aaggaagtac aacaggaaag
     5161 gaagtctgag gatggaagct aaaattggta tgaatttata ttttagagat caaaatgtac
     5221 cttatgttga aacctatgta agaagtgatt atgtagaaag agtgaaagtg atagctctta
     5281 gtctggaaag cccactggct tgtttgggca tttctcatgg cttcccactc aaagtggatc
     5341 cccaaaatca cttgatggat ttccttgctg atttctaagt aaactatggt ttaagaaaga
     5401 aatgacaggg ctcagcactg ccctacagta ccaagaatac aaatgtttcc atgaagtctt
     5461 caaaggcatt tgtaaaattc aggctgtaag tgattagtta gttcattctg cacttattta
     5521 ttaactgtat attcagttcc aggctctagg gtagagatta tggataaagg tgaattagat
     5581 agatgaagtt tttgccctca cagcaaaagc tttagccaat aattaaagct atcactggaa
     5641 gtgggtctgt gccaataacc tagagaagag cagtgctttt agagttgagc tatattccca
     5701 atcagttctt aatggtggtt ttaccccctt ccctctacac tgtcttttct tgagattgga
     5761 tcatgtgtgt gaacccacat catttccagt caaaatcacc aagagaggcc ggacacagtg
     5821 gctcatgcct ataatcccag cactttggga ggcctaaatg ggtgggcctc ttgagtccag
     5881 agtttgagac cagcctgggc gaccccagct ctaccaaaaa aaccaaaaaa caaaaaatac
     5941 caaaaaaaaa aaaaattagg ccgatgaagt gacatgcacc tgtgtgccac ctactcagga
     6001 ggctgaggtg gcaggatcac ttgcatctgg gggcaaaggc tgccgtgagc tgagattgtg
     6061 ccattgcgct ccagcctggg cgacagagca agactctgtg tgtgtgtgtg tgtgtgtgtg
     6121 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtataca cacacacata tatatacaca
     6181 cacccccagg agaccttttg tctttcttgg ttcatgtgcc catcagtgtg ggcaggagaa
     6241 tagaatactc tgtccacact tgagtcttag tcacctttgg agcctaggag tcagccatcc
     6301 tcattcaact acatggatcg agaatgggaa tctgggagaa tttttttatg ggcagattaa
     6361 aaaggaagtt aactatgtaa agtaaggcat tgccttccca ccccatccca cctcagttcg
     6421 tgttaaatta tgaatttttc taagagagtt ctttaatgac atggatagag aggaagctga
     6481 ggtggcagta gagtagggag tggaaggcaa atacataaga tactattaaa ctgtgaagct
     6541 gtttgttaca attccaagat tttataattt ctccaacaaa aaacatttgt attttcttcc
     6601 cagtttaagt tcaatgttaa tttgagaaat aattctactc tcaaacttca tgttatgctg
     6661 acagttcaac tgatgctgtc aaaatgtgaa ttactaactt cagtattctc tattcatctg
     6721 atttttctta aaatgtttgc gtgttaatat actcactagc tttaatgctc tactcttata
     6781 ctggcacctt aattatggta aaaggtaaaa ggttaagtta aaattcccaa tcctagttat
     6841 ttggtcaaac agaagattaa cagaactcca tggcttacca caggaatatt tatcaagaga
     6901 aaaagaagat acctatttga atttacatga cactttctgt gaaatatctg gattaccaga
     6961 ttagtctgaa acaaatgcct tccaaattct acagcgcatg cccaaggcac tctttacagt
     7021 acctgttcgg gtcttttctt ctaattttaa gcacctaccc aaacgtcttc ccaggcttgt
     7081 cataataagc aaataacctc agagcagaca aagtgattca atctaagaac cttgaatttc
     7141 tctccttttc acctcaaaat ttctattctc acactttctt tttgtttctc tttccgagaa
     7201 gtggttctcc atcagcaaat accaactgtt ttctgtcctc tattatttat tccatctctt
     7261 cctcctgatc cctgccccat gttgaacccc cattgcccca ctttgatcct tcagtctctt
     7321 cctcacatcc agaactgtac cagatttact cccttagcac actgattaaa atttatttcc
     7381 atttccttat ggaggaggta ttagagactg atcccagacc tctacaaatt tcattgtggc
     7441 agggtaatgt tatgtgtcca taaaccttgt tccttttctt tcagcctgca gttaggtaga
     7501 gccatgtcat ttatttgtaa ctattagaat atgggtatat gggttgaagt aatgtaggcc
     7561 atttccagac atggcccata acacttctca agagattctt catattctat ctcttcccct
     7621 ccaacatgca cccttcaagg ttgaagatgg caccacacaa gatagaagga gccagtgttc
     7681 caaatgacta ggcagagcag aatgctcccc tccctacacc ctagcaccaa ttggtttgga
     7741 tgtgatagtt gaataataca cttttattgt tttaagacat taatattttg agattaatct
     7801 gcaagtctag ttagtaaaac tcgaactctg agctgtctaa tgcagtagcc tctagccaca
     7861 tgtagctatt taaatttaaa ttttaatttt aattaatcaa aattcaactc agatatacta
     7921 gccacatttc aattgctcag tagccacact aattaaattt aaatttaaat agctacatgt
     7981 ggcttatagc accataccag atagcacaga tgtagactgt ttccatcatc acagaaaatt
     8041 ctattggata gtgctgcctt aactaataag acacaaatgt tcttattttt taaatagtcc
     8101 ctaaaacaaa cgaaaaacaa agaactccct tctctaatgg caaatttcag tttgcctaaa
     8161 agaagactca gaatgctttt cttatattgt tacctctgac tgccactaaa tgaataaatt
     8221 tcctgtcttg ctccttctgc caaacttctt gaatgggtga tctttaactc cttgcaatcc
     8281 tggctttttt ccccagtgct cacctgtaat cagtgttggc cacaactccc acccagatgt
     8341 ctcctaaata gttctctgaa cccacctctg tcctgatcta cagacattca ttccaatgct
     8401 ttagcatttc aaggtcatca aatgactcag tgacatctca gacagtcaga cagaccaggt
     8461 caactatgaa ctatttatgc caaaccttac atggttcatg ttatccttgc tatcccatca
     8521 aaataaccac aggctttttt tttttttttt tttaattttt gagatggagt ctcactctgt
     8581 caccccgact agagtgcagt ggtgcgatct cggctctgct cactgcaacc tccgtctcct
     8641 gggttcaaac agtgctcatt cctcaactcc caagtagctg agattacagg catgcaccat
     8701 cacgcctggc taatttttct atttttagca gagatggggt ttcgccatgt tggccaggct
     8761 ggtctcgaac tcctgacctc aagtgatctg cccgcctcca cctcacaaag ctttgggatt
     8821 acaggcatga gccacggtgc ctggcctgca gcctccttta ttttacaaaa ggcattacca
     8881 cttaaacact gaagatagca cgaatctctg atgtttgcaa gttcagaata aaatggaaat
     8941 gaaaattcat tatgtggcag tctcagtact gagcacaaag gcttttaacc acaagcaaat
     9001 tactgtgcta gccagaccgt gtgagagaac tccaggccag cttgctaaaa ctgagcagga
     9061 gaatttggag agagtccaga ggagagccac agaaacaact gaagcatgac aagaataatt
     9121 gcagttctgt tatagagagg tcatctatat caggctttta tggagtacca tgtggaatgt
     9181 taccacgcag agcatgggga acagctgttg tgactcaaca agagacagga cagaagaaat
     9241 gagaacaggt ggaagatcaa taatttaggt ttgatatggg gaaagatgga acagtcaaag
     9301 ggattcacac tgatttttgg ctgagcagaa ggcagagtct cctccaacgg gcaatgtaag
     9361 tgcccccact catctacacc ccgagaagag ccatcaaact gtattaaact tggaaaaagc
     9421 tatttaactt caagtgtgct gcgagaaaac ttcataatag ttcctaagat gtgctaaaaa
     9481 gtaaagtcca aaaagatcat aaagtctgta gagaagttct aagagtgcag tcagctataa
     9541 aaacctagca atttaatttc ttagaaaaat gtagctggag ttcaaactgt agtaacaaag
     9601 gcaagtaaat taagttgtgg gcaggtgtaa ttaagttaat aggaatggca gggatgaata
     9661 taaatcagaa caggactaac agtttgaaac attagatatt caaatgctca gagctgatga
     9721 gccgagccac tacgctaaca actctgcaaa ccaaaggttt cacaaattgc acaagaacct
     9781 ggcctcgatc ccattgtgtt gctggcataa gattagaagg gcctgttggt tcaattgttg
     9841 ctttggacag aggaggacaa acttgccaca gtgaagaaat catttttaaa aaccaccact
     9901 gtcctttagt gcactaattt ctctaataac taggtgtata ttagaaataa tgatatataa
     9961 gtgcagtata ctccagtttc aacagaatgg tgtcacttct gtaaaatgtt gattttcaga
    10021 ttgtcatact ttctaaatac aaaaatttgt cacagcttcc acatttacaa gatattaaac
    10081 attccataaa atatttgtag aaatatgaaa aaccaatttt tgtagttgcc ttttgaaatg
    10141 tatacaaaat atcccacatg ggcaacatgg taaaacctcg tctctacaaa aaaaaaaaaa
    10201 aaaatacaaa aagttagcca ggcctggtgg tgcacgcctg tggtcccagc tactcgggaa
    10261 gctgaagtga gatcacctgt gcccaggagg tcgaagctgc agtgagctgt gattgcacca
    10321 ctgcactcca gcctgggtga cagagtgaga ctttgtctca aataaacaaa aagagacata
    10381 tacaaaatat attagaaagt tgggttaata tcctacaaac aaagtattac atagtttgca
    10441 caggattatg tgtgtaaaaa gttttaaatt cagtatagct tttatttctg cagaactaaa
    10501 agtagtgcat aaaaataaaa gtgggatgag catatctttt taatttgcta tgcagtaaaa
    10561 agtatctcag tcaacatata ggaaaccagt gccatttctg tatcatttga caccgaaggg
    10621 gtattgtttt ttgttttgtt ttgtttttct tttgaagaaa gacttttatg tcttttattc
    10681 tattcttccg atttggggtt taaattaccc ccaataagtt tgcagaatat tctgattact
    10741 actgcttagg tgatatattt catatgagaa ctatctttac acaatgggaa aaaatatggt
    10801 aaccacttca ttcatcagag aagttgtgtt ttgtttactt gtattttgtt tgctaaatga
    10861 aattggttat ttagactcat ggcatgcttt ttctcaaatg gagatttaaa agtcatttcc
    10921 aatgtccttc taacttctgg ccaattccca gtaggctata gaataaacag ttttcatata
    10981 atagtc
//
