LOCUS       NM_145663               3015 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens DBF4B-CDC7 kinase regulatory subunit (DBF4B),
            transcript variant 1, mRNA.
ACCESSION   NM_145663
VERSION     NM_145663.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Goder,A., Maric,C.A., Rainey,M.D., O'Connor,A., Cazzaniga,C.,
            Shamavu,D., Cadoret,J.C. and Santocanale,C.
  TITLE     DBF4, not DRF1, is the crucial regulator of CDC7 kinase at
            replication forks
  JOURNAL   J Cell Biol 223 (8) (2024)
   PUBMED   38865090
  REMARK    GeneRIF: DBF4, not DRF1, is the crucial regulator of CDC7 kinase at
            replication forks.
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 3015)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3015)
  AUTHORS   Chen,L., Luo,C., Shen,L., Liu,Y., Wang,Q., Zhang,C., Guo,R.,
            Zhang,Y., Xie,Z., Wei,N., Wu,W., Han,J. and Feng,Y.
  TITLE     SRSF1 Prevents DNA Damage and Promotes Tumorigenesis through
            Regulation of DBF4B Pre-mRNA Splicing
  JOURNAL   Cell Rep 21 (12), 3406-3413 (2017)
   PUBMED   29262322
  REMARK    GeneRIF: Knockdown of the exon6-containing isoform (DBF4B-FL)
            significantly inhibits the tumorigenic potential of colon cancer.
            Overexpression of DBF4B-FL protects against DNA damage induced by
            SRSF1 knockdown.
REFERENCE   5  (bases 1 to 3015)
  AUTHORS   Tsuji,T., Lau,E., Chiang,G.G. and Jiang,W.
  TITLE     The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage
            checkpoint control
  JOURNAL   Mol Cell 32 (6), 862-869 (2008)
   PUBMED   19111665
  REMARK    GeneRIF: These results indicate that Ddk functions as an upstream
            regulator to monitor S-phase checkpoint signaling.
REFERENCE   6  (bases 1 to 3015)
  AUTHORS   Yoshizawa-Sugata,N., Ishii,A., Taniyama,C., Matsui,E., Arai,K. and
            Masai,H.
  TITLE     A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required
            for efficient progression of S and M phases
  JOURNAL   J Biol Chem 280 (13), 13062-13070 (2005)
   PUBMED   15668232
  REMARK    GeneRIF: ASKL1 in a complex with Cdc7 may play a role in normal
            progression of both S and M phases
REFERENCE   7  (bases 1 to 3015)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   8  (bases 1 to 3015)
  AUTHORS   Montagnoli,A., Bosotti,R., Villa,F., Rialland,M., Brotherton,D.,
            Mercurio,C., Berthelsen,J. and Santocanale,C.
  TITLE     Drf1, a novel regulatory subunit for human Cdc7 kinase
  JOURNAL   EMBO J 21 (12), 3171-3181 (2002)
   PUBMED   12065429
  REMARK    GeneRIF: Results describe a novel human protein, Drf1, related to
            both human and yeast Dbf4. Drf1 is a nuclear cell cycle-regulated
            protein which binds to Cdc7 and activates the kinase.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC016158.1, AF448801.1 and
            AW290902.1.
            
            On Nov 23, 2018 this sequence version replaced NM_145663.2.
            
            Summary: This gene encodes a regulator of the cell division cycle 7
            homolog (S. cerevisiae) protein, a serine-threonine kinase which
            links cell cycle regulation to genome duplication. This protein
            localizes to the nucleus and, in complex with the cell division
            cycle 7 homolog (S. cerevisiae) protein, may facilitate M phase
            progression. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF448801.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315005.8/ ENSP00000323663.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BC016158.1         58-75
            19-2409             AF448801.1         1-2391
            2410-3015           AW290902.1         12-617              c
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.31"
     gene            1..3015
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /note="DBF4B-CDC7 kinase regulatory subunit"
                     /db_xref="GeneID:80174"
                     /db_xref="HGNC:HGNC:17883"
                     /db_xref="MIM:611661"
     exon            1..175
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..2004
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Dbf4-related factor 1; activator of S-phase kinase-like
                     protein 1; chiffon homolog B; zinc finger, DBF-type
                     containing 1B; ASK-like protein 1; DBF4 homolog B; DBF4
                     zinc finger B"
                     /codon_start=1
                     /product="protein DBF4 homolog B isoform 1"
                     /protein_id="NP_663696.1"
                     /db_xref="CCDS:CCDS11485.1"
                     /db_xref="GeneID:80174"
                     /db_xref="HGNC:HGNC:17883"
                     /db_xref="MIM:611661"
                     /translation="MSEPGKGDDCLELESSMAESRLRAPDLGVSRCLGKCQKNSPGAR
                     KHPFSGKSFYLDLPAGKNLQFLTGAIQQLGGVIEGFLSKEVSYIVSSRREVKAESSGK
                     SHRGCPSPSPSEVRVETSAMVDPKGSHPRPSRKPVDSVPLSRGKELLQKAIRNQGSIS
                     GGGSGGSSSLLTNARSWGVRILHVDEMMMHVQQLSLASLCVKKQQPKKPEGTCPAAES
                     RTRKVARLKAPFLKIEDESRKFRPFHHQFKSFPEISFLGPKDASPFEAPTTLGSMHHT
                     RESKDGEPSPRSAAHTMPRRKKGYCECCQEAFEELHVHLQSAQHRSFALEAHLYAEVD
                     RIIAQLSHSFADIPFQAGLPRWSGSPASDCDPLCPETLHPHQPSHPRAASPRIRKEDS
                     CQASVTQGRAAGQQRWTESLDGVMGPPASHTCVSATTLLPALPKGSREQGCLCPCPAS
                     FTQSHLVTSLALLPGEWSPAEDMPLHPSQENSFAPADIPVKGPLLFPEARPWLMSARC
                     WVRPFPFVTWGCLIPHDTTPLHEEVSPCPCLRLGYLYLLLTQSLWCRVRVPSLSTAGP
                     IPRTSHPCTLAFPSYLNDHDLGHLCQAKPQGWNTPQPFLHCGFLAVDSG"
     exon            176..238
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            239..381
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            382..573
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            574..624
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            625..712
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            713..786
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            787..823
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            824..869
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            870..986
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1095
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1220
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1345
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..3015
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2995..3000
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /note="hexamer: AATAAA"
     polyA_site      3015
                     /gene="DBF4B"
                     /gene_synonym="ASKL1; CHIFB; DRF1; ZDBF1B"
                     /note="major polyA site"
ORIGIN      
        1 aaactgaagc cgcggccgaa aacgccaaga gattgatgct gtagctgccc tgagataacc
       61 aggactgtgg aatcgggaag agctcatgga gctcgcgaat gtaatacgga ggcctctgag
      121 gaaggagtac ggaggccgag aaggagccgg catttgatga gcgaaccggg aaagggagac
      181 gattgcctcg agctggagag ttccatggct gagagtaggc tccgggcccc ggacctagga
      241 gtttccaggt gtctaggaaa atgccagaag aactcaccag gtgccaggaa gcatcccttt
      301 tccggaaagt ccttttactt ggatctgcct gctggcaaga atctccagtt tttgacgggg
      361 gccattcagc aactgggtgg ggtaattgag ggttttctga gcaaagaagt aagttacatc
      421 gtgtccagcc gcagagaagt aaaggcagag agcagtggga aaagccatag aggctgccct
      481 agccctagcc ccagtgaggt cagagtggaa acatcggcca tggttgatcc aaaaggcagc
      541 caccccaggc cttcacggaa acccgttgac tcggtgcctc taagcagagg gaaggagctg
      601 ctgcagaagg ctatcagaaa ccaggggagc atcagtggag gaggcagtgg gggcagcagc
      661 agcctcctga ccaatgcccg ctcttgggga gtgaggattc tgcacgtgga tgaaatgatg
      721 atgcacgtgc aacagctgtc tcttgcgtct ttatgtgtga aaaaacaaca gccaaagaag
      781 ccagagggaa catgtccagc agcagagtca agaacacgga aagtggccag actgaaggcc
      841 ccgttcctca aaatcgaaga tgaaagcagg aagtttcgtc ctttccatca tcagtttaaa
      901 tcctttcctg aaatttcttt tcttggaccc aaagatgcaa gtccctttga ggccccgacg
      961 accctgggca gcatgcacca taccagagaa tccaaggatg gagagccaag cccacgatca
     1021 gctgcccaca ccatgcccag gaggaagaaa ggctactgcg agtgctgtca ggaggccttc
     1081 gaggagctcc atgtgcatct tcagagtgcc cagcaccgga gctttgccct ggaagcccat
     1141 ctatatgcag aagtggacag gatcattgct cagctcagcc acagctttgc agacatccct
     1201 ttccaggctg gcctccccag gtggtcaggt tccccagctt ctgattgtga ccctctctgt
     1261 cctgagactc tgcaccccca tcagccctcc catcccaggg cagcatctcc caggataagg
     1321 aaagaagaca gctgccaggc atcagtgacc caaggcaggg ctgcgggcca gcagcgatgg
     1381 acagaatcac tagatggtgt gatgggacct cctgcaagtc acacatgtgt gagtgccaca
     1441 accctcctgc cggccttgcc caagggctcc agggagcagg gctgcctctg tccctgccca
     1501 gcctccttta cccagtctca tctggtcact tccttggctc tgctgcctgg ggagtggtcg
     1561 cctgcagagg acatgcccct ccatccctcc caagaaaact cctttgcccc ggcggacatt
     1621 cctgttaagg gcccactcct cttccctgaa gccagaccgt ggcttatgtc tgcacgctgc
     1681 tgggttcgtc cctttccttt tgtgacatgg ggttgcctca ttccccatga caccacccct
     1741 ctgcatgagg aagtttcccc ttgcccctgt ctcagacttg gataccttta cctgctgctc
     1801 acacaaagcc tgtggtgccg ggttcgggtg ccctcattgt caactgcagg acccattccc
     1861 cgaacctcac atccgtgtac ccttgccttc ccctcctatc tcaatgatca tgaccttgga
     1921 catctctgcc aggccaaacc ccaaggctgg aacactcctc agccatttct ccattgcggc
     1981 ttcctggctg tagactcagg ttagaggtga acccagaaca cctgagactt gacccaggat
     2041 ggatgggtgc tgcttgatgt gaatgaggtc ccgcagtggc tccttggcgt gagcactgct
     2101 cagactcctt tccactccag ccccctttcc acatcgcacc agatgacttt tacccagacc
     2161 cagtgggcat tgccttatct tgcagtcagt cccttttcaa catgttgccg tttctttctg
     2221 aagaggtgtc ctccctccac aagtcacact gtctgtccct ggccctccag cccacctcgc
     2281 caaccactct tgttggtttc cttctcagac ttgccacctt tcccctctgc cccaaaatgc
     2341 catgctcctc tcctggaaaa cacttgagtt gattcagtaa atcgacttca aatacttgaa
     2401 ggctcccacc ttctgttctc tggctccttc ctgcggtcta tacctaccgc ctcctcttca
     2461 cctccttccc ttccacactt ccttcctggg tagctctgcc tgaagcattc cactaagatc
     2521 atctattcca aggtcatgga caggctactg gtgaccaaag ttggttcctt ttctcctttc
     2581 tttcctcctt gaagcctggc tcccttggtc gcagcagccc ctcagtggcc tggttctcct
     2641 gtccccctgc ccttcctcac cattgcccat tccctcgttc gttcattcag cacaggcctt
     2701 gccgtctgcc ctgagtcagc tccgagacac ctgaagagcc ctccagccct aactacttta
     2761 ctcagactag gtccccaggc ctttgttctt gcctcttctc gctgagcctt tcacttctcg
     2821 gcagatgtga ccgattggta gctccacccc aactcccttc tgctgggtgg aatgcaggag
     2881 ctagctgcct ccaactcact gtgacctcag aaaaatgcct ttattactcg ggcctcagtt
     2941 tcctcgtctt taagtaaggg gcttggatga gatgatttca ggaccctttc caataataaa
     3001 atactgtgac tgcca
//
