LOCUS       NM_006716               3655 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens DBF4-CDC7 kinase regulatory subunit (DBF4), transcript
            variant 1, mRNA.
ACCESSION   NM_006716
VERSION     NM_006716.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Goder,A., Maric,C.A., Rainey,M.D., O'Connor,A., Cazzaniga,C.,
            Shamavu,D., Cadoret,J.C. and Santocanale,C.
  TITLE     DBF4, not DRF1, is the crucial regulator of CDC7 kinase at
            replication forks
  JOURNAL   J Cell Biol 223 (8) (2024)
   PUBMED   38865090
  REMARK    GeneRIF: DBF4, not DRF1, is the crucial regulator of CDC7 kinase at
            replication forks.
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Wu,Z., Zhang,L., Li,X., Liu,L., Kuang,T., Qiu,Z., Deng,W. and
            Wang,W.
  TITLE     The prognostic significance and potential mechanism of DBF4 zinc
            finger in hepatocellular carcinoma
  JOURNAL   Sci Rep 14 (1), 10662 (2024)
   PUBMED   38724606
  REMARK    GeneRIF: The prognostic significance and potential mechanism of
            DBF4 zinc finger in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3655)
  AUTHORS   Zhang,L., Hong,J., Chen,W., Zhang,W., Liu,X., Lu,J., Tang,H.,
            Yang,Z., Zhou,K., Xie,H., Jia,C., Jiang,D. and Zheng,S.
  TITLE     DBF4 Dependent Kinase Inhibition Suppresses Hepatocellular
            Carcinoma Progression and Potentiates Anti-Programmed Cell Death-1
            Therapy
  JOURNAL   Int J Biol Sci 19 (11), 3412-3427 (2023)
   PUBMED   37497004
  REMARK    GeneRIF: DBF4 Dependent Kinase Inhibition Suppresses Hepatocellular
            Carcinoma Progression and Potentiates Anti-Programmed Cell Death-1
            Therapy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3655)
  AUTHORS   Tang,Y., Xu,L., Ren,Y., Li,Y., Yuan,F., Cao,M., Zhang,Y., Deng,M.
            and Yao,Z.
  TITLE     Identification and Validation of a Prognostic Model Based on Three
            MVI-Related Genes in Hepatocellular Carcinoma
  JOURNAL   Int J Biol Sci 18 (1), 261-275 (2022)
   PUBMED   34975331
  REMARK    GeneRIF: Identification and Validation of a Prognostic Model Based
            on Three MVI-Related Genes in Hepatocellular Carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3655)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3655)
  AUTHORS   Yamada,M., Sato,N., Taniyama,C., Ohtani,K., Arai,K. and Masai,H.
  TITLE     A 63-base pair DNA segment containing an Sp1 site but not a
            canonical E2F site can confer growth-dependent and E2F-mediated
            transcriptional stimulation of the human ASK gene encoding the
            regulatory subunit for human Cdc7-related kinase
  JOURNAL   J Biol Chem 277 (31), 27668-27681 (2002)
   PUBMED   12015319
  REMARK    GeneRIF: results suggest that E2F regulates the ASK promoter
            through an atypical mode of recognition of the target site
REFERENCE   7  (bases 1 to 3655)
  AUTHORS   Masai,H., Matsui,E., You,Z., Ishimi,Y., Tamai,K. and Arai,K.
  TITLE     Human Cdc7-related kinase complex. In vitro phosphorylation of MCM
            by concerted actions of Cdks and Cdc7 and that of a criticial
            threonine residue of Cdc7 bY Cdks
  JOURNAL   J Biol Chem 275 (37), 29042-29052 (2000)
   PUBMED   10846177
REFERENCE   8  (bases 1 to 3655)
  AUTHORS   Jiang,W., McDonald,D., Hope,T.J. and Hunter,T.
  TITLE     Mammalian Cdc7-Dbf4 protein kinase complex is essential for
            initiation of DNA replication
  JOURNAL   EMBO J 18 (20), 5703-5713 (1999)
   PUBMED   10523313
REFERENCE   9  (bases 1 to 3655)
  AUTHORS   Lepke,M., Putter,V., Staib,C., Kneissl,M., Berger,C., Hoehn,K.,
            Nanda,I., Schmid,M. and Grummt,F.
  TITLE     Identification, characterization and chromosomal localization of
            the cognate human and murine DBF4 genes
  JOURNAL   Mol Gen Genet 262 (2), 220-229 (1999)
   PUBMED   10517317
REFERENCE   10 (bases 1 to 3655)
  AUTHORS   Kumagai,H., Sato,N., Yamada,M., Mahony,D., Seghezzi,W., Lees,E.,
            Arai,K. and Masai,H.
  TITLE     A novel growth- and cell cycle-regulated protein, ASK, activates
            human Cdc7-related kinase and is essential for G1/S transition in
            mammalian cells
  JOURNAL   Mol Cell Biol 19 (7), 5083-5095 (1999)
   PUBMED   10373557
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB028069.1, BC036045.1 and AC005164.2.
            
            On Nov 23, 2018 this sequence version replaced NM_006716.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036045.1, SRR1803614.91073.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265728.6/ ENSP00000265728.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-286               AB028069.1         278-563
            287-3643            BC036045.1         357-3713
            3644-3655           AC005164.2         31404-31415
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.12"
     gene            1..3655
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /note="DBF4-CDC7 kinase regulatory subunit"
                     /db_xref="GeneID:10926"
                     /db_xref="HGNC:HGNC:17364"
                     /db_xref="MIM:604281"
     exon            1..286
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..2265
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     activator of S phase kinase; zinc finger, DBF-type
                     containing 1; DBF4 homolog; DBF4 zinc finger A; protein
                     DBF4 homolog A; chiffon homolog A; DBF4-type zinc
                     finger-containing protein 1; regulatory subunit of DDK"
                     /codon_start=1
                     /product="protein DBF4 homolog A isoform 1"
                     /protein_id="NP_006707.1"
                     /db_xref="CCDS:CCDS5611.1"
                     /db_xref="GeneID:10926"
                     /db_xref="HGNC:HGNC:17364"
                     /db_xref="MIM:604281"
                     /translation="MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCK
                     PLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRI
                     SPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILS
                     NALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKP
                     FVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGD
                     KYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKL
                     VFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTV
                     KEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQ
                     LPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKS
                     DTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNM
                     DSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQT
                     SEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF"
     exon            287..459
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            460..639
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            640..690
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            691..760
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            761..837
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            838..874
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            875..920
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1049
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1164
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1289
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..3655
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2447..2452
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /note="hexamer: AATAAA"
     polyA_site      2478
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /note="major polyA site"
     regulatory      3623..3628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
                     /note="hexamer: AATAAA"
     polyA_site      3655
                     /gene="DBF4"
                     /gene_synonym="ASK; CHIF; DBF4A; ZDBF1"
ORIGIN      
        1 aaatcttcaa ccgccgcagc ccactcgttt gtgctttgcg ccttcctcct ccgcgccttg
       61 gagccggatc cggccccgga aacccgacct gcagacgcgg tacctctact gcgtagaggc
      121 cgtagctggc ggaaggagag aggcggccgt cctgtcaaca ggccggggga agccgtgctt
      181 tcgcggctgc ccggtgcgac actttctccg gacccagcat gtaggtgccg ggcgactgcc
      241 atgaactccg gagccatgag gatccacagt aaaggacatt tccagggtgg aatccaagtc
      301 aaaaatgaaa aaaacagacc atctctgaaa tctctgaaaa ctgataacag gccagaaaaa
      361 tccaaatgta agccactttg gggaaaagta ttttaccttg acttaccttc tgtcaccata
      421 tctgaaaaac ttcaaaagga cattaaggat ctgggagggc gagttgaaga atttctcagc
      481 aaagatatca gttatcttat ttcaaataag aaggaagcta aatttgcaca aaccttgggt
      541 cgaatttctc ctgtaccaag tccagaatct gcatatactg cagaaaccac ttcacctcat
      601 cccagccatg atggaagttc atttaagtca ccagacacag tgtgtttaag cagaggaaaa
      661 ttattagttg aaaaagctat caaggaccat gattttattc cttcaaatag tatattatca
      721 aatgccttgt catggggagt aaaaattctt catattgatg acattagata ctacattgaa
      781 caaaagaaaa aagagttgta tttactcaag aaatcaagta cttcagtaag agatgggggc
      841 aaaagagttg gtagtggtgc acaaaaaaca agaacaggaa gactcaaaaa gccttttgta
      901 aaggtggaag atatgagcca actttatagg ccattttatc ttcagctgac caatatgcct
      961 tttataaatt attctattca gaagccctgc agtccatttg atgtagacaa gccatctagt
     1021 atgcaaaagc aaactcaggt taaactaaga atccaaacag atggcgataa gtatggtgga
     1081 acctcaattc aactccagtt gaaagagaag aagaaaaaag gatattgtga atgttgcttg
     1141 cagaaatatg aagatctaga aactcacctt ctaagtgagc aacacagaaa ctttgcacag
     1201 agtaaccagt atcaagttgt tgatgatatt gtatctaagt tagtttttga ctttgtggaa
     1261 tatgaaaagg acacacctaa aaagaaaaga ataaaataca gtgttggatc cctttctcct
     1321 gtttctgcaa gtgtcctgaa aaagactgaa caaaaggaaa aagtggaatt gcaacatatt
     1381 tctcagaaag attgccagga agatgataca acagtgaagg agcagaattt cctgtataaa
     1441 gagacccagg aaactgaaaa aaagctcctg tttatttcag agcccatccc ccacccttca
     1501 aatgaattga gagggcttaa tgagaaaatg agtaataaat gttccatgtt aagtacagct
     1561 gaagatgaca taagacagaa ttttacacag ctacctctac ataaaaacaa acaggaatgc
     1621 attcttgaca tttccgaaca cacattaagt gaaaatgact tagaagaact aagggtagat
     1681 cactataaat gtaacataca ggcatctgta catgtttctg atttcagtac agataatagt
     1741 ggatctcaac caaaacagaa gtcagatact gtgctttttc cagcaaagga tctcaaggaa
     1801 aaggaccttc attcaatatt tactcatgat tctggtctga taacaataaa cagttcacaa
     1861 gagcacctaa ctgttcaggc aaaggctcca ttccatactc ctcctgagga acccaatgaa
     1921 tgtgacttca agaatatgga tagtttacct tctggtaaaa tacatcgaaa agtgaaaata
     1981 atattaggac gaaatagaaa agaaaatctg gaaccaaatg ctgaatttga taaaagaact
     2041 gaatttatta cacaagaaga aaacagaatt tgtagttcac cggtacagtc tttactagac
     2101 ttgtttcaga ctagtgaaga gaaatcagaa tttttgggtt tcacaagcta cacagaaaag
     2161 agtggtatat gcaatgtttt agatatttgg gaagaggaaa attcagataa tctgttaaca
     2221 gcgtttttct cgtccccttc aacttctaca tttactggct tttagaattt aaaaaatgca
     2281 tacttttcag aagtgataag gatcatattc ttgaaatttt tataaatatg tatggaaatt
     2341 cttaggattt ttttaccagc tttgtttaca gacccaaatg taaatattaa aaataaatat
     2401 ttgcaatttt ctacagaatt gaatacctgt taaagaaaaa ttacagaata aacttgtgac
     2461 tggtcttgtt ttacattata tatatgttcg taattgtttc ctgagaatta caatgaataa
     2521 taatttgctt tgtcactgaa aaccaccagt gaagtgcaat ttggggaata tcaaacttag
     2581 cattatacat ttggatattc tagttgtatt gtaaatttta aaagattatc aggataacat
     2641 gaccttgcct tgaaaaacct aaatttcata cagaaaggaa aaatacattt ctatgcttga
     2701 attctggaaa tcatcagaaa tattgacact tgagggtcac tggtggacca tgctagtatt
     2761 ttggtgctga gtaattagat tatgtatagg ggtaactgga agccatgaag gaaggactta
     2821 accaggctac caagtggaat ataatttatc tgctattttt aattacttac cttgtaaata
     2881 actttaataa tattcaaaag ttgactctcc tgtggctcat gccacaggat cccagcactt
     2941 tgggaggccg aggcaggctg atcacgaggt cagaagatca agaccatcct ggccaacatg
     3001 gtgaaacctt gtctactaaa aatacaaaaa attagctggg catggcggca tgcgcctgta
     3061 gtcccagcta ctccctccag aggctgagaa ttgcttgaac ctgggaagtg caggttgcag
     3121 tgagccaaga ttgtgccact gtattccaga ctgggtgaca gagtgagact ccatctcaaa
     3181 aaaaaaaaag ttgactctac cccctaattt ggtaggagat gaaggagaaa aggatggcat
     3241 tgaattatag atacagtttt gggatatata caaggattgc cttgatctgg cacttactga
     3301 tacaagcatt tggagaagag aaaattcaaa tataagaaat ttcaatttga atctgtggat
     3361 ataaggcatt tgcctttgag tatgttcttg taggagtaag actaccacca gtgacattca
     3421 attggttcta ttgcatagta catgaatcta ttgtcactaa aaattaggtt actaatctta
     3481 atttttttga aatatatgat tctcagtaca ggactaattc atatgctttt cctgcataca
     3541 ttattttgat catttggata acatcaatga acactggctt tttaacacct ttgtttttca
     3601 taccagtacc tgaagtaggc tcaataaaag agtctttaca gtgcatacac gcaaa
//
