LOCUS       NM_001388484            4272 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens deleted in azoospermia 4 (DAZ4), transcript variant 3,
            mRNA.
ACCESSION   NM_001388484 XM_005262602
VERSION     NM_001388484.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Pineau,C., Hikmet,F., Zhang,C., Oksvold,P., Chen,S., Fagerberg,L.,
            Uhlen,M. and Lindskog,C.
  TITLE     Cell Type-Specific Expression of Testis Elevated Genes Based on
            Transcriptomics and Antibody-Based Proteomics
  JOURNAL   J Proteome Res 18 (12), 4215-4230 (2019)
   PUBMED   31429579
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Ghorbel,M., Baklouti-Gargouri,S., Keskes,R., Chakroun,N.,
            Sellami,A., Fakhfakh,F. and Ammar-Keskes,L.
  TITLE     gr/gr-DAZ2-DAZ4-CDY1b deletion is a high-risk factor for male
            infertility in Tunisian population
  JOURNAL   Gene 592 (1), 29-35 (2016)
   PUBMED   27457284
  REMARK    GeneRIF: study reported a new type of AZFc deletion
            (gr/gr-DAZ2-DAZ4-CDY1b) and hypothesis that this new deletion is
            the result of 2 successive events favored by the unstable structure
            of AZFc region; also demonstrated that this deletion constitutes a
            relative high-risk factor for male infertility in Tunisian
            population
REFERENCE   3  (bases 1 to 4272)
  AUTHORS   Zhang,Y., Li,M., Xiao,F., Teng,R., Zhang,C., Lan,A., Gu,K., Li,J.,
            Wang,D., Li,H., Jiang,L., Zeng,S., He,M., Huang,Y., Guo,P.,
            Zhang,X. and Yang,X.
  TITLE     Impact of partial DAZ1/2 deletion and partial DAZ3/4 deletion on
            male infertility
  JOURNAL   Gene 571 (1), 9-16 (2015)
   PUBMED   26232607
  REMARK    GeneRIF: Studies indicate that partial RNA-binding proteins DAZ1/2
            deletion was associated wih male infertility, but partial
            RNA-binding proteins DAZ3/4 deletion was not associated with male
            infertility.
            Review article
REFERENCE   4  (bases 1 to 4272)
  AUTHORS   Ghorbel,M., Baklouti-Gargouri,S., Keskes,R., Chakroun,N.,
            Sellami,A., Fakhfakh,F. and Ammar-Keskes,L.
  TITLE     Combined deletion of DAZ2 and DAZ4 copies of Y chromosome DAZ gene
            is associated with male infertility in Tunisian men
  JOURNAL   Gene 547 (2), 191-194 (2014)
   PUBMED   24878370
  REMARK    GeneRIF: Oligospermia seems to be promoted by removing DAZ4 copy.
            Deletion of DAZ2 and DAZ4 gene copies is associated with male
            infertility.
REFERENCE   5  (bases 1 to 4272)
  AUTHORS   Kim,B., Lee,Y., Kim,Y., Lee,K.H., Chun,S., Rhee,K., Seo,J.T.,
            Kim,S.W. and Paick,J.S.
  TITLE     Polymorphic expression of DAZ proteins in the human testis
  JOURNAL   Hum Reprod 24 (6), 1507-1515 (2009)
   PUBMED   19223287
  REMARK    GeneRIF: All four DAZ genes are expressed in the human testis, and
            their products are highly polymorphic among men
REFERENCE   6  (bases 1 to 4272)
  AUTHORS   Agulnik,A.I., Zharkikh,A., Boettger-Tong,H., Bourgeron,T.,
            McElreavey,K. and Bishop,C.E.
  TITLE     Evolution of the DAZ gene family suggests that Y-linked DAZ plays
            little, or a limited, role in spermatogenesis but underlines a
            recent African origin for human populations
  JOURNAL   Hum Mol Genet 7 (9), 1371-1377 (1998)
   PUBMED   9700189
REFERENCE   7  (bases 1 to 4272)
  AUTHORS   Yen,P.H., Chai,N.N. and Salido,E.C.
  TITLE     The human DAZ genes, a putative male infertility factor on the Y
            chromosome, are highly polymorphic in the DAZ repeat regions
  JOURNAL   Mamm Genome 8 (10), 756-759 (1997)
   PUBMED   9321470
REFERENCE   8  (bases 1 to 4272)
  AUTHORS   Yen,P.H., Chai,N.N. and Salido,E.C.
  TITLE     The human autosomal gene DAZLA: testis specificity and a candidate
            for male infertility
  JOURNAL   Hum Mol Genet 5 (12), 2013-2017 (1996)
   PUBMED   8968756
REFERENCE   9  (bases 1 to 4272)
  AUTHORS   Saxena,R., Brown,L.G., Hawkins,T., Alagappan,R.K., Skaletsky,H.,
            Reeve,M.P., Reijo,R., Rozen,S., Dinulos,M.B., Disteche,C.M. and
            Page,D.C.
  TITLE     The DAZ gene cluster on the human Y chromosome arose from an
            autosomal gene that was transposed, repeatedly amplified and pruned
  JOURNAL   Nat Genet 14 (3), 292-299 (1996)
   PUBMED   8896558
REFERENCE   10 (bases 1 to 4272)
  AUTHORS   Reijo,R., Lee,T.Y., Salo,P., Alagappan,R., Brown,L.G.,
            Rosenberg,M., Rozen,S., Jaffe,T., Straus,D., Hovatta,O. et al.
  TITLE     Diverse spermatogenic defects in humans caused by Y chromosome
            deletions encompassing a novel RNA-binding protein gene
  JOURNAL   Nat Genet 10 (4), 383-393 (1995)
   PUBMED   7670487
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010089.4, AC006982.3 and
            AC006338.6.
            
            On Nov 12, 2020 this sequence version replaced XM_005262602.1.
            
            Summary: This gene is a member of the DAZ gene family and is a
            candidate for the human Y-chromosomal azoospermia factor (AZF). Its
            expression is restricted to premeiotic germ cells, particularly in
            spermatogonia. It encodes an RNA-binding protein that is important
            for spermatogenesis. Four copies of this gene are found on
            chromosome Y within palindromic duplications; one pair of genes is
            part of the P2 palindrome and the second pair is part of the P1
            palindrome. Each gene contains a 2.4 kb repeat including a 72-bp
            exon, called the DAZ repeat; the number of DAZ repeats is variable
            and there are several variations in the sequence of the DAZ repeat.
            Each copy of the gene also contains a 10.8 kb region that may be
            amplified; this region includes five exons that encode an RNA
            recognition motif (RRM) domain. This gene contains two copies of
            the 10.8 kb repeat. Alternatively spliced transcript variants
            encoding different isoforms have been described. [provided by
            RefSeq, Feb 2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968968,
                              SAMEA2148093 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000682740.1/ ENSP00000508087.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression, longest
                                         protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC010089.4         97456-97666
            212-358             AC006982.3         1112-1258
            359-450             AC006982.3         1533-1624
            451-502             AC006338.6         238-289
            503-566             AC006338.6         726-789
            567-706             AC006338.6         879-1018
            707-853             AC006338.6         10013-10159
            854-945             AC006338.6         10434-10525
            946-997             AC006338.6         11086-11137
            998-1061            AC006338.6         11574-11637
            1062-1201           AC006338.6         11727-11866
            1202-1273           AC006338.6         13267-13338
            1274-1345           AC006338.6         15710-15781
            1346-1417           AC006338.6         18100-18171
            1418-1489           AC006338.6         20496-20567
            1490-1561           AC006338.6         22880-22951
            1562-1633           AC006338.6         25276-25347
            1634-1705           AC006338.6         27653-27724
            1706-1777           AC006338.6         30033-30104
            1778-1849           AC006338.6         32410-32481
            1850-1921           AC006338.6         42198-42269
            1922-1993           AC006338.6         44583-44654
            1994-2065           AC006338.6         46968-47039
            2066-2137           AC006338.6         49352-49423
            2138-2209           AC006338.6         51737-51808
            2210-2281           AC006338.6         54122-54193
            2282-2316           AC006338.6         54939-54973
            2317-2415           AC006338.6         59546-59644
            2416-4272           AC006338.6         63618-65474
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /map="Yq11.23"
     gene            1..4272
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /note="deleted in azoospermia 4"
                     /db_xref="GeneID:57135"
                     /db_xref="HGNC:HGNC:15966"
                     /db_xref="MIM:400048"
     exon            1..211
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..2380
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /note="isoform 3 is encoded by transcript variant 3;
                     deleted in azoospermia protein 4"
                     /codon_start=1
                     /product="deleted in azoospermia protein 4 isoform 3"
                     /protein_id="NP_001375413.1"
                     /db_xref="CCDS:CCDS94720.1"
                     /db_xref="GeneID:57135"
                     /db_xref="HGNC:HGNC:15966"
                     /db_xref="MIM:400048"
                     /translation="MSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFV
                     GGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIH
                     FHGKKLKLGPAIRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNP
                     VTQHVQSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDE
                     TEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLG
                     PAIRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNPVTQHVQAYS
                     AYPHSPGQVITGCQLLVYNYQEYPTYPDSAFQVTTGYQLPVYNYQPFPAYPRSPFQVT
                     AGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQPFPAYPSSPFQVTAGYQLPVYNY
                     QAFPAYPNSPFQVATGYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPNSP
                     FQVATGYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPSSPFQVTTGYQLP
                     VYNYQAFPAYPSSPFQVTTGYQLPVYNYQAFPAYPSSPFQVTTGYQLPVYNYQAFPAY
                     PSSPFQVTTGYQLPVYNYQAFPAYPSSPFQVTTGYQLPVYNYQAFPAYPNSAVQVTTG
                     YQFHVYNYQMPPQCPVGEQRRNLWTEAYKWWYLVCLIQRRD"
     exon            212..358
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            359..450
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            451..502
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            503..566
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            567..706
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            707..853
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            854..945
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            946..997
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1061
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1201
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1273
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1345
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1417
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1489
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1561
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1633
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1705
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1777
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1849
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..1921
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..1993
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2065
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2137
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            2138..2209
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2281
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2316
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2415
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..4272
                     /gene="DAZ4"
                     /gene_synonym="pDP1680; pDP1681"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcggcctg cgctcctcag cctggcggtt ctacctccga gggttcgccc gcccttggtt
       61 ttccttacac cttagccttt ggctcctttg accactcgaa gccccacagc gtgttccagc
      121 ggacttcacc agcagaccca gaagtggtgg gtgaaacact gcctctgttc ctccttgagc
      181 ctgtcgggag ctgctgcctg ccaccaccat gtctgctgca aatcctgaga ctccaaactc
      241 aaccatctcc agagaggcca gcacccagtc ttcatcagct gcagctagcc aaggctgggt
      301 gttaccagaa ggcaaaatcg tgccaaacac tgtttttgtt ggtggaattg atgctaggat
      361 ggatgaaact gagattggaa gctgctttgg tagatacggt tcagtgaaag aagtgaagat
      421 aatcacgaat cgaactggtg tgtccaaagg ctatggattt gtttcgtttg ttaatgacgt
      481 ggatgtccag aagatagtag gatcacagat acatttccat ggtaaaaagc tgaagctggg
      541 ccctgcaatc aggaaacaaa agttatgtgc tcgtcatgtg cagccacgtc ctttggtagt
      601 taatcctcct cctccaccac agtttcagaa cgtctggcgg aatccaaaca ctgaaaccta
      661 cctgcagccc caaatcacgc cgaatcctgt aactcagcac gttcagtctg ctgcaaatcc
      721 tgagactcca aactcaacca tctccagaga ggccagcacc cagtcttcat cagctgcagc
      781 tagccaaggc tgggtgttac cagaaggcaa aatcgtgcca aacactgttt ttgttggtgg
      841 aattgatgct aggatggatg aaactgagat tggaagctgc tttggtagat acggttcagt
      901 gaaagaagtg aagataatca cgaatcgaac tggtgtgtcc aaaggctatg gatttgtttc
      961 gtttgttaat gacgtggatg tccagaagat agtaggatca cagatacatt tccatggtaa
     1021 aaagctgaag ctgggccctg caatcaggaa acaaaagtta tgtgctcgtc atgtgcagcc
     1081 acgtcctttg gtagttaatc ctcctcctcc accacagttt cagaacgtct ggcggaatcc
     1141 aaacactgaa acctacctgc agccccaaat cacgccgaat cctgtaactc agcacgttca
     1201 ggcttactct gcttatccac attcaccagg tcaggtcatc actggatgtc agttgcttgt
     1261 atataattat caggaatatc ctacttatcc cgattcagca tttcaggtca ccactggata
     1321 tcagttgcct gtatataatt atcagccatt tcctgcttat ccaagatcac catttcaggt
     1381 cactgctgga tatcagttgc ctgtatataa ttatcaggca tttcctgctt atccaaattc
     1441 accatttcaa gtcgccactg gatatcagtt ccctgtatac aattatcagc catttcctgc
     1501 ttatccaagt tcaccatttc aggtcactgc tggatatcag ttgcctgtat ataattatca
     1561 ggcatttcct gcttatccaa attcaccatt tcaagtcgcc actggatatc agttccctgt
     1621 atacaattat caggcatttc ctgcttatcc aaattcacca gttcaggtca ccactggata
     1681 tcagttgcct gtatacaatt atcaggcatt tcctgcttat ccaaattcac catttcaagt
     1741 cgccactgga tatcagttcc ctgtatacaa ttatcaggca tttcctgctt atccaaattc
     1801 accagttcag gtcaccactg gatatcagtt gcctgtatac aattatcagg catttcctgc
     1861 ttatccaagt tcaccatttc aggtcaccac tggatatcag ttgcctgtat ataattatca
     1921 ggcatttcct gcttatccaa gttcaccatt tcaggtcacc actggatatc agttgcctgt
     1981 atataattat caggcatttc ctgcttatcc aagttcacca tttcaggtca ccactggata
     2041 tcagttgcct gtatataatt atcaggcatt tcctgcttat ccaagttcac catttcaggt
     2101 caccactgga tatcagttgc ctgtatataa ttatcaggca tttcctgctt atccaagttc
     2161 accatttcag gtcaccactg gatatcagtt gcctgtatat aattatcagg catttcctgc
     2221 ttatccaaat tcagcagttc aggtcaccac tggatatcag ttccatgtat acaattacca
     2281 gatgccaccg cagtgccctg ttggggagca aaggagaaat ctgtggaccg aagcatacaa
     2341 atggtggtat cttgtctgtt taatccagag aagagactga taaattccgt tgttactcaa
     2401 gatgactgct tcaagggtaa aagagtgcat cgctttagaa gaagtttggc agtatttaaa
     2461 tctgttggat cctctcagct atctagtttc atgggaagtt gctggttttg aatattaagc
     2521 taaaagtttt ccactattac agaaattctg aattttggta aatcacactg aaactttctg
     2581 tataacttgt attattagac tctctagttt tatcttaaca ctgaaactgt tcttcattag
     2641 atgtttattt agaacctggt tctgtgttta atatatagtt taaagtaaca aataatcgag
     2701 actgaaagaa tgttaagatt tatctgcaag gatttttaaa aaattgaaac ttgcatttta
     2761 agtgtttaaa agcaaatact gactttcaaa aaagttttta aaacctgatt tgaaagctaa
     2821 caattttgat agtctgaaca caagcatttc acttctccaa gaagtacctg tgaacagtac
     2881 aatatttcag tattgagctt tgcatttatg atttatctag aaatttacct caaaagcaga
     2941 atttttaaaa ctgcattttt aatcagtgga actcaatgta tagttagctt tattgaagtc
     3001 ttatccaaac ccagtaaaac agattctaag caaacagtcc aatcagtgag tcataatgtt
     3061 tattcaaagt attttatctt ttatctagaa tccacatatg tatgtccaat ttgattggga
     3121 tagtagttag gataactaaa attctgggcc taatttttta aagaatccaa gacaaactaa
     3181 actttactgg gtatataacc ttctcaatga gttaccattc ttttttataa aaaaaattgt
     3241 tccttgaaat gctaaactta atggctgtat gtgaaatttg caaaatactg gtattaaaga
     3301 acgctgcagc ttttttatgt cactcaaagg ttaatcggag tatctgaaag gaattgtttt
     3361 tataaaaaca ttgaagtatt agttacttgc tataaataga tttttatttt tgttttttag
     3421 cctgttatat ttccttctgt aaaataaaat atgtccagaa gaggcatgtt gtttctagat
     3481 taggtagtgt cctcatttta tattgtgacc acacagctag agcaccagag cccttttgct
     3541 atactcacag tcttgttttc ccagcctctt ttactagtct ttcaggaggt ttgctcttag
     3601 aactggtgat gtaaagaatg gaagtagctg tatgagcagt tcaaaggcca agccgtggaa
     3661 tggtagcaat gggatataat acctttctaa gggaaacatt tgtatcagta tcatttgatc
     3721 tgccatggac atgtgtttaa agtggctttc tggcccttct ttcaatggct tcttccctaa
     3781 aacgtggaga ctctaagtta atgtcgttac tatgggccat attactaatg cccactgggg
     3841 tctatgattt ctcaaaattt tcattcggaa tccgaaggat acagtcttta aactttagaa
     3901 ttcccaagaa ggctttatta cacctcagaa attgaaagca ccatgacttt gtccattaaa
     3961 aaattatcca tagttttttt agtgctttta acattccgac atacatcatt ctgtgattaa
     4021 atctccagat ttctgtaaat gatacctaca ttctaaagag ttaattctaa ttattccgat
     4081 atgaccttaa ggaaaagtaa aggaataaat ttttgtcttt gttgaagtat ttaatagagt
     4141 aaggtaaaga agatattaag tccctttcaa aatggaaaat taattctaaa ctgagaaaaa
     4201 tgttcctact acctattgct gatactgtct ttgcataaat gaataaaaat aaactttttt
     4261 tcttcaaatg tg
//
