LOCUS       NM_004081               4335 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens deleted in azoospermia 1 (DAZ1), transcript variant 1,
            mRNA.
ACCESSION   NM_004081
VERSION     NM_004081.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ghorbel,M., Baklouti-Gargouri,S., Keskes,R., Sellami,A.,
            McElreavy,K. and Ammar-Keskes,L.
  TITLE     Y-chromosome haplogroups and Azoospermia Factor (AZF) analysis in
            Tunisian infertile male
  JOURNAL   Hum Fertil (Camb) 26 (5), 1238-1247 (2023)
   PUBMED   36591797
  REMARK    GeneRIF: Y-chromosome haplogroups and Azoospermia Factor (AZF)
            analysis in Tunisian infertile male.
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Wang,J., Peng,Z., Guo,J., Wang,Y., Wang,S., Jiang,H., Wang,M.,
            Xie,Y., Li,X., Hu,M., Xie,Y., Cheng,H., Li,T., Jia,L., Song,J.,
            Wang,Y., Hou,J. and Liu,Z.
  TITLE     CXCL10 Recruitment of gammadelta T Cells into the Hypoxic Bone
            Marrow Environment Leads to IL17 Expression and Multiple Myeloma
            Progression
  JOURNAL   Cancer Immunol Res 11 (10), 1384-1399 (2023)
   PUBMED   37586075
  REMARK    GeneRIF: CXCL10 Recruitment of gammadelta T Cells into the Hypoxic
            Bone Marrow Environment Leads to IL17 Expression and Multiple
            Myeloma Progression.
REFERENCE   3  (bases 1 to 4335)
  AUTHORS   Nongthombam,P.D. and Malini,S.S.
  TITLE     Association of DAZL polymorphisms and DAZ deletion with male
            infertility: a systematic review and meta-analysis
  JOURNAL   Genes Genomics 45 (6), 709-722 (2023)
   PUBMED   36434389
  REMARK    GeneRIF: Association of DAZL polymorphisms and DAZ deletion with
            male infertility: a systematic review and meta-analysis.
            Review article
REFERENCE   4  (bases 1 to 4335)
  AUTHORS   Lardone,M.C., Ortega,V., Ortiz,E., Florez,M., Piottante,A.,
            Ebensperger,M., Flores,S., Pezo,P., Orellana,M., Moraga,M. and
            Castro,A.
  TITLE     Partial-AZFc deletions in Chilean men with primary spermatogenic
            impairment: gene dosage and Y-chromosome haplogroups
  JOURNAL   J Assist Reprod Genet 37 (12), 3109-3119 (2020)
   PUBMED   33034826
  REMARK    GeneRIF: Partial-AZFc deletions in Chilean men with primary
            spermatogenic impairment: gene dosage and Y-chromosome haplogroups.
REFERENCE   5  (bases 1 to 4335)
  AUTHORS   Pineau,C., Hikmet,F., Zhang,C., Oksvold,P., Chen,S., Fagerberg,L.,
            Uhlen,M. and Lindskog,C.
  TITLE     Cell Type-Specific Expression of Testis Elevated Genes Based on
            Transcriptomics and Antibody-Based Proteomics
  JOURNAL   J Proteome Res 18 (12), 4215-4230 (2019)
   PUBMED   31429579
REFERENCE   6  (bases 1 to 4335)
  AUTHORS   Saxena,R., Brown,L.G., Hawkins,T., Alagappan,R.K., Skaletsky,H.,
            Reeve,M.P., Reijo,R., Rozen,S., Dinulos,M.B., Disteche,C.M. and
            Page,D.C.
  TITLE     The DAZ gene cluster on the human Y chromosome arose from an
            autosomal gene that was transposed, repeatedly amplified and pruned
  JOURNAL   Nat Genet 14 (3), 292-299 (1996)
   PUBMED   8896558
REFERENCE   7  (bases 1 to 4335)
  AUTHORS   Reijo,R., Seligman,J., Dinulos,M.B., Jaffe,T., Brown,L.G.,
            Disteche,C.M. and Page,D.C.
  TITLE     Mouse autosomal homolog of DAZ, a candidate male sterility gene in
            humans, is expressed in male germ cells before and after puberty
  JOURNAL   Genomics 35 (2), 346-352 (1996)
   PUBMED   8661148
REFERENCE   8  (bases 1 to 4335)
  AUTHORS   Cooke,H.J., Lee,M., Kerr,S. and Ruggiu,M.
  TITLE     A murine homologue of the human DAZ gene is autosomal and expressed
            only in male and female gonads
  JOURNAL   Hum Mol Genet 5 (4), 513-516 (1996)
   PUBMED   8845845
REFERENCE   9  (bases 1 to 4335)
  AUTHORS   Reijo,R., Lee,T.Y., Salo,P., Alagappan,R., Brown,L.G.,
            Rosenberg,M., Rozen,S., Jaffe,T., Straus,D., Hovatta,O. et al.
  TITLE     Diverse spermatogenic defects in humans caused by Y chromosome
            deletions encompassing a novel RNA-binding protein gene
  JOURNAL   Nat Genet 10 (4), 383-393 (1995)
   PUBMED   7670487
REFERENCE   10 (bases 1 to 4335)
  AUTHORS   Fan,Y. and Silber,S.J.
  TITLE     Y Chromosome Infertility
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301513
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC053490.2 and AC010088.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 19, 2020 this sequence version replaced NM_004081.6.
            
            Summary: This gene is a member of the DAZ gene family and is a
            candidate for the human Y-chromosomal azoospermia factor (AZF). Its
            expression is restricted to premeiotic germ cells, particularly in
            spermatogonia. It encodes an RNA-binding protein that is important
            for spermatogenesis. Four copies of this gene are found on
            chromosome Y within palindromic duplications; one pair of genes is
            part of the P2 palindrome and the second pair is part of the P1
            palindrome. Each gene contains a 2.4 kb repeat including a 72-bp
            exon, called the DAZ repeat; the number of DAZ repeats is variable
            and there are several variations in the sequence of the DAZ repeat.
            Each copy of the gene also contains a 10.8 kb region that may be
            amplified; this region includes five exons that encode an RNA
            recognition motif (RRM) domain. This gene contains three copies of
            the 10.8 kb repeat. However, no transcripts containing three copies
            of the RRM domain have been described; thus the RefSeq for this
            gene contains only two RRM domains. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005362.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968968,
                                           SAMEA2142586 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000405239.6/ ENSP00000384573.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC053490.2         63151-63361         c
            212-358             AC053490.2         56405-56551         c
            359-450             AC053490.2         56039-56130         c
            451-502             AC053490.2         55427-55478         c
            503-566             AC053490.2         54927-54990         c
            567-706             AC053490.2         54698-54837         c
            707-853             AC053490.2         45557-45703         c
            854-945             AC053490.2         45191-45282         c
            946-997             AC053490.2         44579-44630         c
            998-1061            AC053490.2         44079-44142         c
            1062-1201           AC053490.2         43850-43989         c
            1202-1348           AC053490.2         34717-34863         c
            1349-1440           AC053490.2         34351-34442         c
            1441-1492           AC053490.2         33739-33790         c
            1493-1556           AC053490.2         33239-33302         c
            1557-1696           AC053490.2         33010-33149         c
            1697-1768           AC053490.2         31538-31609         c
            1769-1840           AC053490.2         29093-29164         c
            1841-1912           AC053490.2         26702-26773         c
            1913-1984           AC053490.2         24306-24377         c
            1985-2056           AC053490.2         21923-21994         c
            2057-2128           AC053490.2         19527-19598         c
            2129-2200           AC053490.2         17150-17221         c
            2201-2272           AC053490.2         7364-7435           c
            2273-2344           AC053490.2         4979-5050           c
            2345-2379           AC053490.2         4200-4234           c
            2380-2478           AC010088.4         107015-107113       c
            2479-4335           AC010088.4         101195-103051       c
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="Y"
                     /map="Yq11.223"
     gene            1..4335
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /note="deleted in azoospermia 1"
                     /db_xref="GeneID:1617"
                     /db_xref="HGNC:HGNC:2682"
                     /db_xref="MIM:400003"
     exon            1..211
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..2443
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /note="isoform 1 is encoded by transcript variant 1;
                     deleted in azoospermia protein 1; testicular tissue
                     protein Li 49"
                     /codon_start=1
                     /product="deleted in azoospermia protein 1 isoform 1"
                     /protein_id="NP_004072.3"
                     /db_xref="CCDS:CCDS48209.1"
                     /db_xref="GeneID:1617"
                     /db_xref="HGNC:HGNC:2682"
                     /db_xref="MIM:400003"
                     /translation="MSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFV
                     GGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIH
                     FHGKKLKLGPAIRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNP
                     VTQHVQSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDE
                     TEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLG
                     PAIRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNPVTQHVQSAA
                     NPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGR
                     YGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLC
                     ARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNPVTQHVQAYSAYPHSPGQV
                     ITGCQLLVYNYQEYPTYPDSAFQVTTGYQLPVYNYQPFPAYPRSPFQVTAGYQLPVYN
                     YQAFPAYPNSPFQVATGYQFPVYNYQPFPAYPSSPFQVTAGYQLPVYNYQAFPAYPNS
                     PFQVATGYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPSSPFQVTTGYQL
                     PVYNYQAFPAYPNSAVQVTTGYQFHVYNYQMPPQCPVGEQRRNLWTEAYKWWYLVCLI
                     QRRD"
     exon            212..358
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            359..450
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            451..502
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            503..566
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            567..706
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            707..853
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            854..945
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            946..997
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1061
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1201
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1348
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1440
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1492
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1556
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1696
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1768
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1840
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1912
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..1984
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2056
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2128
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2200
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2272
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2344
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2379
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2478
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..4335
                     /gene="DAZ1"
                     /gene_synonym="DAZ; SPGY"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcggcctg cgctcctcag cctggcggtt ctacctccga gggttcgccc gcccttggtt
       61 ttccttacac cttagccttt ggctcctttg accactcgaa gccccacagc gtgttccagc
      121 ggacttcacc agcagaccca gaagtggtgg gtgaaacact gcctctgttc ctccttgagc
      181 ctgtcgggag ctgctgcctg ccaccaccat gtctgctgca aatcctgaga ctccaaactc
      241 aaccatctcc agagaggcca gcacccagtc ttcatcagct gcagctagcc aaggctgggt
      301 gttaccagaa ggcaaaatcg tgccaaacac tgtttttgtt ggtggaattg atgctaggat
      361 ggatgaaact gagattggaa gctgctttgg tagatacggt tcagtgaaag aagtgaagat
      421 aatcacgaat cgaactggtg tgtccaaagg ctatggattt gtttcgtttg ttaatgacgt
      481 ggatgtccag aagatagtag gatcacagat acatttccat ggtaaaaagc tgaagctggg
      541 ccctgcaatc aggaaacaaa agttatgtgc tcgtcatgtg cagccacgtc ctttggtagt
      601 taatcctcct cctccaccac agtttcagaa cgtctggcgg aatccaaaca ctgaaaccta
      661 cctgcagccc caaatcacgc cgaatcctgt aactcagcac gttcagtctg ctgcaaatcc
      721 tgagactcca aactcaacca tctccagaga ggccagcacc cagtcttcat cagctgcagc
      781 tagccaaggc tgggtgttac cagaaggcaa aatcgtgcca aacactgttt ttgttggtgg
      841 aattgatgct aggatggatg aaactgagat tggaagctgc tttggtagat acggttcagt
      901 gaaagaagtg aagataatca cgaatcgaac tggtgtgtcc aaaggctatg gatttgtttc
      961 gtttgttaat gacgtggatg tccagaagat agtaggatca cagatacatt tccatggtaa
     1021 aaagctgaag ctgggccctg caatcaggaa acaaaagtta tgtgctcgtc atgtgcagcc
     1081 acgtcctttg gtagttaatc ctcctcctcc accacagttt cagaacgtct ggcggaatcc
     1141 aaacactgaa acctacctgc agccccaaat cacgccgaat cctgtaactc agcacgttca
     1201 gtctgctgca aatcctgaga ctccaaactc aaccatctcc agagaggcca gcacccagtc
     1261 ttcatcagct gcagctagcc aaggctgggt gttaccagaa ggcaaaatcg tgccaaacac
     1321 tgtttttgtt ggtggaattg atgctaggat ggatgaaact gagattggaa gctgctttgg
     1381 tagatacggt tcagtgaaag aagtgaagat aatcacgaat cgaactggtg tgtccaaagg
     1441 ctatggattt gtttcgtttg ttaatgacgt ggatgtccag aagatagtag gatcacagat
     1501 acatttccat ggtaaaaagc tgaagctggg ccctgcaatc aggaaacaaa agttatgtgc
     1561 tcgtcatgtg cagccacgtc ctttggtagt taatcctcct cctccaccac agtttcagaa
     1621 cgtctggcgg aatccaaaca ctgaaaccta cctgcagccc caaatcacgc cgaatcctgt
     1681 aactcagcac gttcaggctt actctgctta tccacattca ccaggtcagg tcatcactgg
     1741 atgtcagttg cttgtatata attatcagga atatcctact tatcccgatt cagcatttca
     1801 ggtcaccact ggatatcagt tgcctgtata taattatcag ccatttcctg cttatccaag
     1861 atcaccattt caggtcactg ctggatatca gttgcctgta tataattatc aggcatttcc
     1921 tgcttatcca aattcaccat ttcaagtcgc cactggatat cagttccctg tatacaatta
     1981 tcagccattt cctgcttatc caagttcacc atttcaggtc actgctggat atcagttgcc
     2041 tgtatataat tatcaggcat ttcctgctta tccaaattca ccatttcaag tcgccactgg
     2101 atatcagttc cctgtataca attatcaggc atttcctgct tatccaaatt caccagttca
     2161 ggtcaccact ggatatcagt tgcctgtata caattatcag gcatttcctg cttatccaag
     2221 ttcaccattt caggtcacca ctggatatca gttgcctgta tataattatc aggcatttcc
     2281 tgcttatcca aattcagcag ttcaggtcac cactggatat cagttccatg tatacaatta
     2341 ccagatgcca ccgcagtgcc ctgttgggga gcaaaggaga aatctgtgga ccgaagcata
     2401 caaatggtgg tatcttgtct gtttaatcca gagaagagac tgataaattc cgttgttact
     2461 caagatgact gcttcaaggg taaaagagtg catcgcttta gaagaagttt ggcagtattt
     2521 aaatctgttg gatcctctca gctatctagt ttcatgggaa gttgctggtt ttgaatatta
     2581 agctaaaagt tttccactat tacagaaatt ctgaattttg gtaaatcaca ctgaaacttt
     2641 ctgtataact tgtattatta gactctctag ttttatctta acactgaaac tgttcttcat
     2701 tagatgttta tttagaacct ggttctgtgt ttaatatata gtttaaagta acaaataatc
     2761 gagactgaaa gaatgttaag atttatctgc aaggattttt aaaaaattga aacttgcatt
     2821 ttaagtgttt aaaagcaaat actgactttc aaaaaagttt ttaaaacctg atttgaaagc
     2881 taacaatttt gatagtctga acacaagcat ttcacttctc caagaagtac ctgtgaacag
     2941 tacaatattt cagtattgag ctttgcattt atgatttatc tagaaattta cctcaaaagc
     3001 agaattttta aaactgcatt tttaatcagt ggaactcaat gtatagttag ctttattgaa
     3061 gtcttatcca aacccagtaa aacagattct aagcaaacag tccaatcagt gagtcataat
     3121 gtttattcaa agtattttat cttttatcta gaatccacat atgtatgtcc aatttgattg
     3181 ggatagtagt taggataact aaaattctgg gcctaatttt ttaaagaatc caagacaaac
     3241 taaactttac tgggtatata accttctcaa tgagttacca ttctttttta taaaaaaaat
     3301 tgttccttga aatgctaaac ttaatggctg tatgtgaaat ttgcaaaata ctggtattaa
     3361 agaacgctgc agctttttta tgtcactcaa aggttaatcg gagtatctga aaggaattgt
     3421 ttttataaaa acattgaagt attagttact tgctataaat agatttttat ttttgttttt
     3481 tagcctgtta tatttccttc tgtaaaataa aatatgtcca gaagaggcat gttgtttcta
     3541 gattaggtag tgtcctcatt ttatattgtg accacacagc tagagcacca gagccctttt
     3601 gctatactca cagtcttgtt ttcccagcct cttttactag tctttcagga ggtttgctct
     3661 tagaactggt gatgtaaaga atggaagtag ctgtatgagc agttcaaagg ccaagccgtg
     3721 gaatggtagc aatgggatat aatacctttc taagggaaac atttgtatca gtatcatttg
     3781 atctgccatg gacatgtgtt taaagtggct ttctggccct tctttcaatg gcttcttccc
     3841 taaaacgtgg agactctaag ttaatgtcgt tactatgggc catattacta atgcccactg
     3901 gggtctatga tttctcaaaa ttttcattcg gaatccgaag gatacagtct ttaaacttta
     3961 gaattcccaa gaaggcttta ttacacctca gaaattgaaa gcaccatgac tttgtccatt
     4021 aaaaaattat ccatagtttt tttagtgctt ttaacattcc gacatacatc attctgtgat
     4081 taaatctcca gatttctgta aatgatacct acattctaaa gagttaattc taattattcc
     4141 gatatgacct taaggaaaag taaaggaata aatttttgtc tttgttgaag tatttaatag
     4201 agtaaggtaa agaagatatt aagtcccttt caaaatggaa aattaattct aaactgagaa
     4261 aaatgttcct actacctatt gctgatactg tctttgcata aatgaataaa aataaacttt
     4321 ttttcttcaa atgtg
//
