LOCUS       NM_006133               5799 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens diacylglycerol lipase alpha (DAGLA), mRNA.
ACCESSION   NM_006133
VERSION     NM_006133.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Yan,Y.C., Meng,G.X., Yang,C.C., Yang,Y.F., Tan,S.Y., Yan,L.J.,
            Ding,Z.N., Ma,Y.L., Dong,Z.R. and Li,T.
  TITLE     Diacylglycerol lipase alpha promotes hepatocellular carcinoma
            progression and induces lenvatinib resistance by enhancing YAP
            activity
  JOURNAL   Cell Death Dis 14 (7), 404 (2023)
   PUBMED   37414748
  REMARK    GeneRIF: Diacylglycerol lipase alpha promotes hepatocellular
            carcinoma progression and induces lenvatinib resistance by
            enhancing YAP activity.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5799)
  AUTHORS   Okubo,Y., Kasamatsu,A., Yamatoji,M., Fushimi,K., Ishigami,T.,
            Shimizu,T., Kasama,H., Shiiba,M., Tanzawa,H. and Uzawa,K.
  TITLE     Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer
            by cell-cycle progression
  JOURNAL   Exp Cell Res 367 (1), 112-118 (2018)
   PUBMED   29614312
  REMARK    GeneRIF: Overexpression of DAGLA in human oral squamous cell
            carcinomas cells controlled cell proliferation through cell-cycle
            progression.
REFERENCE   4  (bases 1 to 5799)
  AUTHORS   Ishiguro,H., Higuchi,S., Arinami,T. and Onaivi,E.S.
  TITLE     Association between alcoholism and the gene encoding the
            endocannabinoid synthesizing enzyme diacylglycerol lipase alpha in
            the Japanese population
  JOURNAL   Alcohol 68, 59-62 (2018)
   PUBMED   29477030
  REMARK    GeneRIF: Our findings indicated the involvement of DAGLA in
            alcoholism, possibly by its genetic dysfunction and also by the
            influence of stress
REFERENCE   5  (bases 1 to 5799)
  AUTHORS   Smith,D.R., Stanley,C.M., Foss,T., Boles,R.G. and McKernan,K.
  TITLE     Rare genetic variants in the endocannabinoid system genes CNR1 and
            DAGLA are associated with neurological phenotypes in humans
  JOURNAL   PLoS One 12 (11), e0187926 (2017)
   PUBMED   29145497
  REMARK    GeneRIF: phenotypes associated with rare CNR1 variants are
            reminiscent of those implicated in the theory of clinical
            endocannabinoid deficiency syndrome. The severe phenotypes
            associated with rare DAGLA variants underscore the critical role of
            rapid 2-AG synthesis and the endocannabinoid system in regulating
            neurological function and development
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5799)
  AUTHORS   Kennedy,R.B., Ovsyannikova,I.G., Pankratz,V.S., Haralambieva,I.H.,
            Vierkant,R.A. and Poland,G.A.
  TITLE     Genome-wide analysis of polymorphisms associated with cytokine
            responses in smallpox vaccine recipients
  JOURNAL   Hum Genet 131 (9), 1403-1421 (2012)
   PUBMED   22610502
REFERENCE   7  (bases 1 to 5799)
  AUTHORS   Lemaitre,R.N., Tanaka,T., Tang,W., Manichaikul,A., Foy,M.,
            Kabagambe,E.K., Nettleton,J.A., King,I.B., Weng,L.C.,
            Bhattacharya,S., Bandinelli,S., Bis,J.C., Rich,S.S., Jacobs,D.R.
            Jr., Cherubini,A., McKnight,B., Liang,S., Gu,X., Rice,K.,
            Laurie,C.C., Lumley,T., Browning,B.L., Psaty,B.M., Chen,Y.D.,
            Friedlander,Y., Djousse,L., Wu,J.H., Siscovick,D.S.,
            Uitterlinden,A.G., Arnett,D.K., Ferrucci,L., Fornage,M., Tsai,M.Y.,
            Mozaffarian,D. and Steffen,L.M.
  TITLE     Genetic loci associated with plasma phospholipid n-3 fatty acids: a
            meta-analysis of genome-wide association studies from the CHARGE
            Consortium
  JOURNAL   PLoS Genet 7 (7), e1002193 (2011)
   PUBMED   21829377
REFERENCE   8  (bases 1 to 5799)
  AUTHORS   Knight,M.A., Hernandez,D., Diede,S.J., Dauwerse,H.G., Rafferty,I.,
            van de Leemput,J., Forrest,S.M., Gardner,R.J., Storey,E., van
            Ommen,G.J., Tapscott,S.J., Fischbeck,K.H. and Singleton,A.B.
  TITLE     A duplication at chromosome 11q12.2-11q12.3 is associated with
            spinocerebellar ataxia type 20
  JOURNAL   Hum Mol Genet 17 (24), 3847-3853 (2008)
   PUBMED   18801880
REFERENCE   9  (bases 1 to 5799)
  AUTHORS   Jung,K.M., Mangieri,R., Stapleton,C., Kim,J., Fegley,D.,
            Wallace,M., Mackie,K. and Piomelli,D.
  TITLE     Stimulation of endocannabinoid formation in brain slice cultures
            through activation of group I metabotropic glutamate receptors
  JOURNAL   Mol Pharmacol 68 (5), 1196-1202 (2005)
   PUBMED   16051747
REFERENCE   10 (bases 1 to 5799)
  AUTHORS   Bisogno,T., Howell,F., Williams,G., Minassi,A., Cascio,M.G.,
            Ligresti,A., Matias,I., Schiano-Moriello,A., Paul,P.,
            Williams,E.J., Gangadharan,U., Hobbs,C., Di Marzo,V. and Doherty,P.
  TITLE     Cloning of the first sn1-DAG lipases points to the spatial and
            temporal regulation of endocannabinoid signaling in the brain
  JOURNAL   J Cell Biol 163 (3), 463-468 (2003)
   PUBMED   14610053
  REMARK    GeneRIF: This gene was functionally characterized as a
            diacylglycerol-lipase (DAGL), specifically referred to as DAGL
            alpha.  This enzyme may play a role in the biosynthesis of the
            endocannbinoid 2-arachidonoylglycerol (2-AG).
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP002380.3 and BC150176.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006133.2.
            
            Summary: This gene encodes a diacylglycerol lipase. The encoded
            enzyme is involved in the biosynthesis of the endocannabinoid
            2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150176.1, BC152453.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000257215.10/ ENSP00000257215.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                AP002380.3         1395-1436
            43-155              BC150176.1         1-113
            156-221             AP002380.3         41157-41222
            222-1681            BC150176.1         180-1639
            1682-1682           AP002380.3         58700-58700
            1683-4725           BC150176.1         1641-4683
            4726-5411           AP002380.3         66932-67617
            5412-5799           BC150176.1         5366-5753
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.2"
     gene            1..5799
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /note="diacylglycerol lipase alpha"
                     /db_xref="GeneID:747"
                     /db_xref="HGNC:HGNC:1165"
                     /db_xref="MIM:614015"
     exon            1..114
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            115..253
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..3287
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /EC_number="3.1.1.116"
                     /note="neural stem cell-derived dendrite regulator;
                     sn1-specific diacylglycerol lipase alpha; DGL-alpha;
                     DAGL-alpha"
                     /codon_start=1
                     /product="diacylglycerol lipase-alpha"
                     /protein_id="NP_006124.1"
                     /db_xref="CCDS:CCDS31578.1"
                     /db_xref="GeneID:747"
                     /db_xref="HGNC:HGNC:1165"
                     /db_xref="MIM:614015"
                     /translation="MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLV
                     YNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRL
                     AILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPT
                     GRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEI
                     AYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTK
                     YLDLKNSQEMLRYKEVCYYMLFALAAYGWPMYLMRKPACGLCQLARSCSCCLCPARPR
                     FAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKK
                     VVISIRGTLSPKDALTDLTGDAERLPVEGHHGTWLGHKGMVLSAEYIKKKLEQEMVLS
                     QAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDA
                     MEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKS
                     ELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEE
                     PTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVS
                     PTEVDLTPELIFQQQPLPTGPPMPTGLALELPTADHRNSSVRSKSQSEMSLEGFSEGR
                     LLSPVVAAAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSS
                     GFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPL
                     EAALGSGGVTPERPPSAAANDEEEEVGGGGGGPASRGELALHNGRLGDSPSPQVLEFA
                     EFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYAEGPKSPSQQEILLRAQFEPNLVPKP
                     PRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGAS
                     PAKQDELVISAR"
     exon            254..465
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            466..567
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            568..706
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            707..794
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            795..929
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1007
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1132
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1286
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1370
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1448
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1529
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1672
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1741
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1814
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..2011
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2141
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2329
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..5799
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5779..5784
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /note="hexamer: TATAAA"
     polyA_site      5799
                     /gene="DAGLA"
                     /gene_synonym="C11orf11; DAGL(ALPHA); DAGLALPHA; NOC2;
                     NSDDR"
                     /note="major polyA site"
ORIGIN      
        1 cttcgcgccg gcgccggcgc cctgcggaag cggcgttagt gaatcggggc cttggggagc
       61 ccaggatgga ggtggcggtc gcggcggcgg gccgagccct gcggcgggcg ggaggaggtg
      121 agcaccaggc ccactgagcc tctgcagagc caccagccat gcccgggatc gtggtgttcc
      181 ggcggcgctg gtctgtgggc agtgatgacc tcgtcctacc ggccatcttc ctctttctcc
      241 tgcataccac ctggtttgtg atcctgtccg tggtgctctt cggcctggtc tataacccgc
      301 acgaggcctg ctccctgaac ctggtggacc acggccgcgg ctacctgggc atcctgctga
      361 gctgcatgat cgctgagatg gccatcatct ggctgagcat gcgcgggggc atcctctaca
      421 cggagccccg tgactccatg cagtacgtgc tctacgtgcg cctggccatc ctggtgatcg
      481 agttcatcta cgccatcgtg ggcatcgtct ggctcactca gtactacacc tcctgcaacg
      541 acctcactgc caagaatgtc accctcggaa tggttgtctg caactgggta gtcatcctca
      601 gtgtgtgcat cactgtcctc tgcgtcttcg accccacggg ccgcaccttt gtcaagctga
      661 gagccaccaa gaggaggcag cgtaacctgc ggacctacaa cctgcggcac cgcttagagg
      721 agggtcaagc caccagctgg tcgcgccggc tcaaagtgtt cctctgctgc acgcggacga
      781 aggactccca gtcagatgcc tactcagaaa tcgcctacct ctttgcggag ttcttccggg
      841 accttgacat tgtgccatcc gacatcattg ctggcctggt gctgctccgg cagcggcagc
      901 gggccaagcg caacgccgtg ctggacgagg caaacaatga catcttggcc ttcctgtctg
      961 ggatgccggt gaccagaaac accaagtacc tcgacctcaa gaattcacaa gagatgctcc
     1021 gctacaaaga ggtctgctac tacatgctct ttgccctggc tgcctacggg tggcccatgt
     1081 acctgatgcg gaagcccgcc tgcggcctct gccaactggc tcggtcctgc tcgtgttgcc
     1141 tgtgtcctgc gaggccgcgg ttcgcccctg gagtcaccat cgaggaagac aactgctgtg
     1201 gctgtaatgc cattgccatc cggcgccact tcctggacga gaacatgact gcggtggaca
     1261 tcgtctatac ctcctgccat gatgcggtct atgaaacgcc cttctacgtg gcggtggacc
     1321 atgacaagaa gaaagtggtg atcagtatcc gggggaccct gtcccccaag gatgccctga
     1381 ctgacctgac gggtgatgct gagcgcctcc ccgtggaggg gcaccacggc acctggctgg
     1441 gccacaaggg tatggtcctc tcagctgagt acatcaagaa gaaactggag caggagatgg
     1501 tcctgtccca ggcctttggg cgagacctgg gccgcggaac caaacactac ggcctgattg
     1561 tggtgggcca ctccctgggc gcgggcactg ctgccatcct ctccttcctt ctgcgcccac
     1621 agtatccgac cctcaagtgc tttgcctact ccccgccagg gggcctgctg agtgaggatg
     1681 cgatggagta ttccaaggag ttcgtgactg ctgtggttct gggcaaagac ctcgtcccca
     1741 ggattggcct ctctcagctg gaaggcttcc gcagacagct cctggatgtc ctgcagcgaa
     1801 gcaccaagcc caaatggcgg atcatcgtgg gggccaccaa atgcatcccc aagtcggagc
     1861 tgcctgagga ggtagaggtg accaccctgg ccagcacgcg gctctggacc caccccagcg
     1921 acctaactat agccctctca gccagcactc cactctaccc gcccggccgc atcatccacg
     1981 tggtccacaa ccaccctgca gagcagtgct gctgctgtga gcaggaggag cccacatact
     2041 ttgccatctg gggcgacaac aaggccttca atgaggtgat catctcgcca gccatgctgc
     2101 atgagcacct gccctatgtg gtcatggagg ggctcaacaa ggtgctggag aactacaaca
     2161 aggggaagac cgctctgctc tctgcagcca aggtcatggt gagccctacc gaggtggacc
     2221 tgactcctga gctcatcttc cagcagcagc cactccccac ggggccgccc atgcccactg
     2281 gccttgccct ggagctgccg actgcagacc accgcaacag cagcgtcagg agcaagtccc
     2341 agtctgagat gagcctggag ggcttctcgg aggggcggct gctgtcgcca gtggttgcgg
     2401 cggcggcccg ccaggacccg gtggagctgc tgctgctgtc tacccaggag cggctggcgg
     2461 cggagctgca ggcccggcgg gcaccactgg ccaccatgga gagcctctcg gacactgagt
     2521 ccctgtacag cttcgactcg cgccgctcct caggcttccg cagcatccgg ggctccccca
     2581 gcctccacgc tgtgctggag cgtgatgaag gccacctctt ctacattgac cctgccatcc
     2641 ccgaggaaaa cccatccctg agctcgcgca ctgagctgct ggcggccgac agcctgtcca
     2701 agcactcaca ggacacgcag cccctggagg cggccctggg cagtggcggc gtcactcctg
     2761 agcggccccc cagtgctgcg gccaatgacg aggaggaaga ggttggcggt gggggtggcg
     2821 ggccggcctc ccgcggggag ctggcgctgc acaatgggcg cctgggggac tcgcccagtc
     2881 ctcaggtgct ggaattcgcc gagttcatcg acagcctctt caacctggac agcaagagca
     2941 gctccttcca agacctctac tgcatggtgg tgcccgagag ccccaccagt gactacgctg
     3001 agggccccaa gtcccccagc cagcaagaga tcctgctccg tgcccagttc gagcccaacc
     3061 tggtgcccaa gcccccacgg ctctttgccg gctcagccga cccctcctcg ggcatctcac
     3121 tctcgccctc cttcccgctc agctcctcgg gtgagctcat ggacctgacg cccacgggcc
     3181 tcagtagcca ggaatgcctg gcggctgaca agatccggac ttctaccccc actggccacg
     3241 gagccagccc cgccaagcaa gatgagctgg tcatctcagc acgctagcac cccagttgcg
     3301 tggccagccg ggcccaggca ggagcaggtg gccctgtggg cacctggtgc ctgccccctg
     3361 ccgggcagct ttaaggacag acccccaggg gcagtttagc ctcaggcaca ggcatcgctg
     3421 ctgagctggg ggtccgcatc cctacctcag cttaggaccc ccagagccaa ggtggctggg
     3481 atctggcccc acagatgggg aaagatgggg aagggtgtgg agtggggagg agcctgggca
     3541 gcctgctggg tgggccacac tcagcctgac tgccctccat gggggcattc tggcaccccc
     3601 tgctccagga caggccatgg gcaagctgcc tcccatcact gcctgctggc tgctctccca
     3661 ggggccaggt ggagagcagt gccccccgac acatgtattc tcatctgtgg tccaggccgg
     3721 catcgtcctg gccacccccc agatctggtg cctgctggcc ggccccctgg ggtgcccctg
     3781 ccgaggtggc ctgcagtgct gtacatgttt acagaagctg ctgggcttgg ctcaggatgt
     3841 gttctgggct tgcaagcccc ccgcccaatc atgtgttcag tagccgtcct ctgagcaggg
     3901 cccaaggcag ccaggggcct ggaggggcca gaggagggtg gggtcagggc cgccccttct
     3961 ctgccttgtg cctctcatgc tgcctcctct gcccatgggt cctgggcacc caggcctgcc
     4021 ctgcctgctg gctacttcct ggcttacctt ctacccccaa ggatcctcac cacccaaagg
     4081 gtggtgggca ctgctgtgac caccccagct gcagagtcag tgccctgggt ggaaggaagg
     4141 cactgagagc ccccttcctc tgagggcccc acctcacccc ttggtgtcac ccccaccacg
     4201 cctaggcagc tctgggccct gggatctgga accaacacac ccctgttccc ctcagctttc
     4261 cctcctcgct ggcctgggca ccctcctggg agcaggcctt cctccctccc acccccaatg
     4321 tcctgttggt aggaggtggg gccaagagtg gggtatggtg ggccttggct ggagacctct
     4381 gtccactgcc cagggagggg cctggggctg ggagcagtcc cggtttagcc tgaggtcccc
     4441 atagggcttc ctcccctgct gggtttggga agcagttagg gagatagcga cccggagttt
     4501 ccccagaagc ggggtgggag ggtgtgcatg ctagtgttgg cgcgtatgca tgtgcatgag
     4561 tgtgcaccgt tcctaaggaa ggggcctctg gggctgccca ccctacctgc cctgcctgcc
     4621 tgctgcccct cccagcctgc caagaaaacg gtaggggagc atgatggggc ctttgaggca
     4681 gggtcgcagg gacaagctca gctttaggca ccatctgttc ccatcgcgcc tgctgctgtg
     4741 acccgttttg gaaaactggt gtgtaccgag gcgctgactg cacggctgac cgcctgctcg
     4801 tgccttcatt ctgcagcggc atggtccctc ccattctggc tccacctgca gcctccctgg
     4861 gtggcctagg ctcccccgac caagagacct ccctctcatg atcactggta cctgggggcc
     4921 tgaattctgg cccccggctc cccacacagc tgggactggc ctggatggct gtcctgggag
     4981 cccctgccca ccctgacaga gggagctggg cctcccctca tcctctgtaa ctcccgcctt
     5041 caccagactc aaggacaccc tggccctgct gaggcataca gagcttcagc ccagcacaga
     5101 agcaagacaa aatcagtggc tcttagagtt tagaaaacaa gacagactct cagatgaaag
     5161 atctgacaag caccgtggcc agtcacaggg agagacttga tgtctggcct tttaattcct
     5221 cctctgccag ggtgggtcct gggacctcta atgtgggcat gtcgtccacc ccaggacgag
     5281 ccatcaggga cagacccccc acccccaagg ctgcagccac accatgtttc aggcttgggg
     5341 ctggggcagg cttgggctca atcctgggca cccaggggca gcccacccct aacctggctc
     5401 ctacccacct cgcccttgaa ggatgggcct gctgcacgtc tccctcctcc accccatacc
     5461 acactggggg gtctgagcca cccccctcag ccccgttcgg ctcagaccga cccccactcc
     5521 atccccagac ctgcagcaca agtgcgcggg cctgtcctcc caggggcctg ggcgactcca
     5581 tatgcaatca gtagcgagca gccgggcccc acagaccctc atgcactctc ttacgtgcca
     5641 ttctccccag actttttttg tacttaatgt atgaaagatc caaactaata ttgctgtaaa
     5701 aaggagagac aaattaatat agcttattct ataaatatat ctgtatataa aggtttctgt
     5761 atattgtata gagctgtgta taaactggat gtagaagca
//
