LOCUS       NM_001344                684 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens defender against cell death 1 (DAD1), mRNA.
ACCESSION   NM_001344
VERSION     NM_001344.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Lampson,B.L., Ramiotarez,A.S., Baro,M., He,L., Hegde,M., Koduri,V.,
            Pfaff,J.L., Hanna,R.E., Kowal,J., Shirole,N.H., He,Y., Doench,J.G.,
            Contessa,J.N., Locher,K.P. and Kaelin,W.G. Jr.
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an
            oligosaccharyltransferase required for inflammatory signaling to
            NF-kappaB
  JOURNAL   Cell 187 (9), 2209-2223 (2024)
   PUBMED   38670073
REFERENCE   2  (bases 1 to 684)
  AUTHORS   Gemmer,M., Chaillet,M.L., van Loenhout,J., Cuevas Arenas,R.,
            Vismpas,D., Grollers-Mulderij,M., Koh,F.A., Albanese,P.,
            Scheltema,R.A., Howes,S.C., Kotecha,A., Fedry,J. and Forster,F.
  TITLE     Visualization of translation and protein biogenesis at the ER
            membrane
  JOURNAL   Nature 614 (7946), 160-167 (2023)
   PUBMED   36697828
REFERENCE   3  (bases 1 to 684)
  AUTHORS   Ramirez,A.S., Kowal,J. and Locher,K.P.
  TITLE     Cryo-electron microscopy structures of human
            oligosaccharyltransferase complexes OST-A and OST-B
  JOURNAL   Science 366 (6471), 1372-1375 (2019)
   PUBMED   31831667
REFERENCE   4  (bases 1 to 684)
  AUTHORS   Pires,A.O., Queiroz,G.A., de Jesus Silva,M., da Silva,R.R., da
            Silva,H.B.F., Carneiro,N.V.Q., Fonseca,H.F., de Santana,M.B.R.,
            Nascimento,R.S., Alcantara-Neves,N.M., Costa,G.N.O., Costa,R.D.S.,
            Barreto,M.L. and Figueiredo,C.A.
  TITLE     Polymorphisms in the DAD1 and OXA1L genes are associated with
            asthma and atopy in a South American population
  JOURNAL   Mol Immunol 101, 294-302 (2018)
   PUBMED   30032071
  REMARK    GeneRIF: variants in both the DAD1 and OXA1L genes may affect atopy
            and asthma in a Latin American population with a high prevalence of
            asthma.
REFERENCE   5  (bases 1 to 684)
  AUTHORS   Qi,W., Fu,R., Wang,H., Liu,C., Ren,Y., Shao,Y. and Shao,Z.
  TITLE     Comparative proteomic analysis of CD34(+) cells in bone marrow
            between severe aplastic anemia and normal control
  JOURNAL   Cell Immunol 304-305, 9-15 (2016)
   PUBMED   27086042
  REMARK    GeneRIF: this study shows that abnormal expression of DAD1 may
            relate to the hyperfunction of immune responses and excessive
            apoptosis of severe aplastic anemia CD34(+) cells
REFERENCE   6  (bases 1 to 684)
  AUTHORS   Kelleher,D.J. and Gilmore,R.
  TITLE     DAD1, the defender against apoptotic cell death, is a subunit of
            the mammalian oligosaccharyltransferase
  JOURNAL   Proc Natl Acad Sci U S A 94 (10), 4994-4999 (1997)
   PUBMED   9144178
REFERENCE   7  (bases 1 to 684)
  AUTHORS   Makishima,T., Nakashima,T., Nagata-Kuno,K., Fukushima,K., Iida,H.,
            Sakaguchi,M., Ikehara,Y., Komiyama,S. and Nishimoto,T.
  TITLE     The highly conserved DAD1 protein involved in apoptosis is required
            for N-linked glycosylation
  JOURNAL   Genes Cells 2 (2), 129-141 (1997)
   PUBMED   9167970
REFERENCE   8  (bases 1 to 684)
  AUTHORS   Apte,S.S., Mattei,M.G., Seldin,M.F. and Olsen,B.R.
  TITLE     The highly conserved defender against the death 1 (DAD1) gene maps
            to human chromosome 14q11-q12 and mouse chromosome 14 and has plant
            and nematode homologs
  JOURNAL   FEBS Lett 363 (3), 304-306 (1995)
   PUBMED   7737422
REFERENCE   9  (bases 1 to 684)
  AUTHORS   Yulug,I.G., See,C.G., Fisher,E.M. and Ylug,I.G.
  TITLE     The DAD1 protein, whose defect causes apoptotic cell death, maps to
            human chromosome 14
  JOURNAL   Genomics 26 (2), 433-435 (1995)
   PUBMED   7601483
  REMARK    Erratum:[Genomics 1995 Jul 1;28(1):129]
REFERENCE   10 (bases 1 to 684)
  AUTHORS   Nakashima,T., Sekiguchi,T., Kuraoka,A., Fukushima,K., Shibata,Y.,
            Komiyama,S. and Nishimoto,T.
  TITLE     Molecular cloning of a human cDNA encoding a novel protein, DAD1,
            whose defect causes apoptotic cell death in hamster BHK21 cells
  JOURNAL   Mol Cell Biol 13 (10), 6367-6374 (1993)
   PUBMED   8413235
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP198316.1, AC243965.2 and
            AK223129.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001344.3.
            
            Summary: DAD1, the defender against apoptotic cell death, was
            initially identified as a negative regulator of programmed cell
            death in the temperature sensitive tsBN7 cell line.  The DAD1
            protein disappeared in temperature-sensitive cells following a
            shift to the nonpermissive temperature, suggesting that loss of the
            DAD1 protein triggered apoptosis.  DAD1 is believed to be a tightly
            associated subunit of oligosaccharyltransferase both in the intact
            membrane and in the purified enzyme, thus reflecting the essential
            nature of N-linked glycosylation in eukaryotes. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK223129.1, SRR5189655.198726.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142586, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000250498.9/ ENSP00000250498.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                BP198316.1         14-59
            47-47               AC243965.2         177627-177627       c
            48-409              BP198316.1         61-422
            410-410             AC243965.2         163551-163551       c
            411-565             BP198316.1         424-578
            566-684             AK223129.1         566-684
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..684
                     /gene="DAD1"
                     /gene_synonym="OST2"
                     /note="defender against cell death 1"
                     /db_xref="GeneID:1603"
                     /db_xref="HGNC:HGNC:2664"
                     /db_xref="MIM:600243"
     exon            1..278
                     /gene="DAD1"
                     /gene_synonym="OST2"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..409
                     /gene="DAD1"
                     /gene_synonym="OST2"
                     /EC_number="2.4.99.18"
                     /note="oligosaccharyltransferase 2 homolog; DAD-1;
                     oligosaccharyl transferase subunit DAD1;
                     oligosaccharyltransferase subunit 2 (non-catalytic)"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit DAD1"
                     /protein_id="NP_001335.1"
                     /db_xref="CCDS:CCDS9571.1"
                     /db_xref="GeneID:1603"
                     /db_xref="HGNC:HGNC:2664"
                     /db_xref="MIM:600243"
                     /translation="MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGY
                     CLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFAST
                     ILHLVVMNFVG"
     exon            279..453
                     /gene="DAD1"
                     /gene_synonym="OST2"
                     /inference="alignment:Splign:2.1.0"
     exon            454..684
                     /gene="DAD1"
                     /gene_synonym="OST2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      634..639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="DAD1"
                     /gene_synonym="OST2"
                     /note="hexamer: ATTAAA"
     polyA_site      684
                     /gene="DAD1"
                     /gene_synonym="OST2"
                     /note="major polyA site"
ORIGIN      
        1 acatccggtg tggtcgacgg gtcctccaag agtttggggc gcggaccgga gtaccttgcg
       61 tgcagttatg tcggcgtcgg tagtgtctgt catttcgcgg ttcttagaag agtacttgag
      121 ctccactccg cagcgtctga agttgctgga cgcgtacctg ctgtatatac tgctgaccgg
      181 ggcgctgcag ttcggttact gtctcctcgt ggggaccttc cccttcaact cttttctctc
      241 gggcttcatc tcttgtgtgg ggagtttcat cctagcggtt tgcctgagaa tacagatcaa
      301 cccacagaac aaagcggatt tccaaggcat ctccccagag cgagcctttg ctgattttct
      361 ctttgccagc accatcctgc accttgttgt catgaacttt gttggctgaa tcattctcat
      421 ttacttaatt gaggagtagg agactaaaag aatgttcact ctttgaattt cctggataag
      481 agttctggag atggcagctt attggacaca tggattttct tcagatttgc acttactgct
      541 agctctgctt tttatgcagg agaaaagccc agagttcact gtgtgtcaga acaactttct
      601 aacaaacatt tattaatcca gcctctgcct ttcattaaat gtaacctttt gccttccaaa
      661 ttaaagaact ccatgccact cctc
//
