LOCUS       NM_004227               4482 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens cytohesin 3 (CYTH3), transcript variant 1, mRNA.
ACCESSION   NM_004227
VERSION     NM_004227.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 4482)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 4482)
  AUTHORS   Fu,Y., Li,J., Feng,M.X., Yang,X.M., Wang,Y.H., Zhang,Y.L., Qin,W.,
            Xia,Q. and Zhang,Z.G.
  TITLE     Cytohesin-3 is upregulated in hepatocellular carcinoma and
            contributes to tumor growth and vascular invasion
  JOURNAL   Int J Clin Exp Pathol 7 (5), 2123-2132 (2014)
   PUBMED   24966920
  REMARK    GeneRIF: Cytohesin-3 was upregulated in hepatocellular carcinoma
            tissues, correlating with overall survival/relapse-free survival
            and tumor size/vascular invasion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4482)
  AUTHORS   Malaby,A.W., van den Berg,B. and Lambright,D.G.
  TITLE     Structural basis for membrane recruitment and allosteric activation
            of cytohesin family Arf GTPase exchange factors
  JOURNAL   Proc Natl Acad Sci U S A 110 (35), 14213-14218 (2013)
   PUBMED   23940353
REFERENCE   6  (bases 1 to 4482)
  AUTHORS   Nevrivy,D.J., Peterson,V.J., Avram,D., Ishmael,J.E., Hansen,S.G.,
            Dowell,P., Hruby,D.E., Dawson,M.I. and Leid,M.
  TITLE     Interaction of GRASP, a protein encoded by a novel retinoic
            acid-induced gene, with members of the cytohesin family of guanine
            nucleotide exchange factors
  JOURNAL   J Biol Chem 275 (22), 16827-16836 (2000)
   PUBMED   10828067
REFERENCE   7  (bases 1 to 4482)
  AUTHORS   Ogasawara,M., Kim,S.C., Adamik,R., Togawa,A., Ferrans,V.J.,
            Takeda,K., Kirby,M., Moss,J. and Vaughan,M.
  TITLE     Similarities in function and gene structure of cytohesin-4 and
            cytohesin-1, guanine nucleotide-exchange proteins for
            ADP-ribosylation factors
  JOURNAL   J Biol Chem 275 (5), 3221-3230 (2000)
   PUBMED   10652308
REFERENCE   8  (bases 1 to 4482)
  AUTHORS   Venkateswarlu,K., Gunn-Moore,F., Oatey,P.B., Tavare,J.M. and
            Cullen,P.J.
  TITLE     Nerve growth factor- and epidermal growth factor-stimulated
            translocation of the ADP-ribosylation factor-exchange factor GRP1
            to the plasma membrane of PC12 cells requires activation of
            phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology
            domain
  JOURNAL   Biochem J 335 (Pt 1) (Pt 1), 139-146 (1998)
   PUBMED   9742223
REFERENCE   9  (bases 1 to 4482)
  AUTHORS   Franco,M., Boretto,J., Robineau,S., Monier,S., Goud,B., Chardin,P.
            and Chavrier,P.
  TITLE     ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP
            ribosylation factor 1, is involved in the control of Golgi
            structure and function
  JOURNAL   Proc Natl Acad Sci U S A 95 (17), 9926-9931 (1998)
   PUBMED   9707577
REFERENCE   10 (bases 1 to 4482)
  AUTHORS   Klarlund,J.K., Guilherme,A., Holik,J.J., Virbasius,J.V., Chawla,A.
            and Czech,M.P.
  TITLE     Signaling by phosphoinositide-3,4,5-trisphosphate through proteins
            containing pleckstrin and Sec7 homology domains
  JOURNAL   Science 275 (5308), 1927-1930 (1997)
   PUBMED   9072969
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009412.6, CB988199.1,
            AJ223957.1, BG620295.1 and BC028717.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004227.3.
            
            Summary: This gene encodes a member of the PSCD (pleckstrin
            homology, Sec7 and coiled-coil domains) family. PSCD family members
            have identical structural organization that consists of an
            N-terminal coiled-coil motif, a central Sec7 domain, and a
            C-terminal pleckstrin homology (PH) domain. The coiled-coil motif
            is involved in homodimerization, the Sec7 domain contains
            guanine-nucleotide exchange protein (GEP) activity, and the PH
            domain interacts with phospholipids and is responsible for
            association of PSCDs with membranes. Members of this family appear
            to mediate the regulation of protein sorting and membrane
            trafficking. This encoded protein is involved in the control of
            Golgi structure and function, and it may have a physiological role
            in regulating ADP-ribosylation factor protein 6 (ARF) functions, in
            addition to acting on ARF1. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028717.1, SRR1803616.73428.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000350796.8/ ENSP00000297044.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC009412.6         44782-44794         c
            14-112              CB988199.1         33-131
            113-1148            AJ223957.1         21-1056
            1149-1216           BG620295.1         346-413
            1217-4482           BC028717.1         1240-4505
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..4482
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /note="cytohesin 3"
                     /db_xref="GeneID:9265"
                     /db_xref="HGNC:HGNC:9504"
                     /db_xref="MIM:605081"
     exon            1..151
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1317
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /note="isoform a is encoded by transcript variant 1;
                     pleckstrin homology, Sec7 and coiled-coil domains 3; ARF
                     nucleotide-binding site opener 3; general receptor of
                     phosphoinositides 1; PH, SEC7 and coiled-coil
                     domain-containing protein 3"
                     /codon_start=1
                     /product="cytohesin-3 isoform a"
                     /protein_id="NP_004218.1"
                     /db_xref="CCDS:CCDS5346.1"
                     /db_xref="GeneID:9265"
                     /db_xref="HGNC:HGNC:9504"
                     /db_xref="MIM:605081"
                     /translation="MDEDGGGEGGGVPEDLSLEEREELLDIRRRKKELIDDIERLKYE
                     IAEVMTEIDNLTSVEESKTTQRNKQIAMGRKKFNMDPKKGIQFLIENDLLQSSPEDVA
                     QFLYKGEGLNKTVIGDYLGERDEFNIKVLQAFVELHEFADLNLVQALRQFLWSFRLPG
                     EAQKIDRMMEAFASRYCLCNPGVFQSTDTCYVLSFAIIMLNTSLHNHNVRDKPTAERF
                     IAMNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGG
                     RVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSH
                     KGQVIKACKTEADGRVVEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRK
                     RRIANKK"
     exon            152..234
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            235..299
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            300..366
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            367..485
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            486..566
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            567..679
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            680..828
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            829..940
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1017
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1089
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1244
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..4482
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4457..4462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /note="hexamer: AATAAA"
     polyA_site      4482
                     /gene="CYTH3"
                     /gene_synonym="ARNO3; cytohesin-3; GRP1; PSCD3"
                     /note="major polyA site"
ORIGIN      
        1 ccgggccgcg cgctgaggag ccgcccggtc gcctgcgcgc tccctccggc ggcgtcccca
       61 gcccgcggcc cctctgctgc cggcccccgg ctcgccggct gcgggagtgg cctcaagatg
      121 gatgaagacg gcggcggcga gggtggtggc gtgcctgaag acctctcatt agaagagaga
      181 gaagaacttc tagacattcg tcgaagaaaa aaggaactta ttgatgacat tgagaggctg
      241 aaatatgaaa ttgcagaggt gatgacagag atcgacaatc taacttccgt agaggagagc
      301 aaaacgactc agaggaacaa acagatagcc atgggaagaa agaaattcaa catggatccc
      361 aaaaagggaa ttcagtttct aatagaaaat gacctgctac agagttcccc agaagacgtc
      421 gcccagttcc tttataaagg agaaggccta aataagaccg tcattgggga ctacctgggt
      481 gaaagggatg aatttaatat taaagttctt caagcctttg ttgaactcca tgagtttgct
      541 gatctcaacc ttgtacaagc cttaaggcag ttcttatgga gcttcaggct gcccggggag
      601 gcgcagaaga ttgatcgcat gatggaggct ttcgcttctc gctactgcct gtgcaacccc
      661 ggggtcttcc agtccacaga cacgtgctac gtgctgtcat tcgccatcat catgctcaac
      721 accagcctcc acaaccacaa cgtgcgtgac aagcccacgg cagaacggtt catcgccatg
      781 aaccgcggca tcaacgaggg cggggacctc cctgaggagc tgctgaggaa tttgtatgag
      841 agcattaaga acgagccatt taagatcccg gaggacgacg ggaacgacct gacccacacc
      901 ttcttcaacc ccgaccgcga gggctggctc ctgaagctgg gagggcgtgt gaagacctgg
      961 aagcgccggt ggttcatcct gaccgataac tgcctctatt actttgaata cacaacagat
     1021 aaggagccca ggggaatcat cccgttggaa aacctcagca tcagggaggt ggaggacccc
     1081 cggaaaccca actgttttga gctctacaat cccagccaca aagggcaggt catcaaggcc
     1141 tgtaagactg aggccgacgg ccgcgtggta gaggggaacc atgtggtgta ccggatctca
     1201 gccccgagcc cggaggagaa ggaggagtgg atgaaatcca tcaaagccag tatcagcaga
     1261 gatcccttct atgacatgtt ggcaacgagg aaacgaagga ttgccaataa aaaatagctt
     1321 tcctggctaa aagacccagg taaaagaccc aaccccagca gaaagacacc gcgggcggcc
     1381 cctccgcgga aggcgtggca gggaggcagt cgccctgcgg tgcaagctgc tgctccagag
     1441 cataccgtgg cccaggtggt atccccaagg cctcgtgccg tggctggggt cctgggaggt
     1501 ggtcgccctg cagtgcaagc tgctgctcca gagcgtaccg tggcccagac tgatcctcga
     1561 ggcctcctgc cgtggctggg gtcatggtcg gctgcgcatg tccagaagca tttccttcct
     1621 gcgaccatcc cggcgcccct agggggagaa gccaggacag cagcttccgc tgtctccaca
     1681 gcagacacgg gacggattcc acagacggga gcctcattcg taccatgcca aacgcattca
     1741 ctcggggcag tattaaccgt tctagaaagc cactgtttta tagcaaaaca ggaaaggaaa
     1801 agctaccagt tttttattca gaatttttct cagatatata ggattatagc ttttatatgc
     1861 ctttttatat tctgaaatta taacaaaaga tactttctaa cagtagtatt tttagaatgg
     1921 cagctataaa gttaactcct ggacacaagt atatactgtg cactgaaaaa atatccatct
     1981 acacagcacc caaggggagg gctgggggca ccggcacggg ggcagcgtgc agccctgccc
     2041 tgtcaggctg tcagacaagc cccggggggc agcaggtggg ctcgggacgg gctgggggag
     2101 ggacggccat ggcacttggg ggctccaggg tgactcccat gaggcctccc ttcaaccagg
     2161 ctttttggcc ccacaaatac tttaagcaaa tcattaaaat tataacagtt aatggtttgg
     2221 gggtgtttag gctgtaactg ctaactccta ggaaacagcc ttttccctgg acacagatgg
     2281 tccatacgct gagccacgtg aaactgctga tgttttgttt agatgcacac acatggcagc
     2341 gtttcataca ggtcagcagg ttagaccggc ttttgaccat attcatcgct atttaaaacc
     2401 tgtggcaaaa tgaacgctta ttttacagac tttctaattt gaccagattt cttaatgaat
     2461 agacacagaa ttaactaaaa acagtctcac cccatgtagt gcgccgtgtc ctgagagagg
     2521 tgccctccct acgaggaggg aagaacaggc cctggggtgc agaggcccgg cacgtagaga
     2581 acccagatag acgccggtgg tggaactggt caaactccac gcccgcctgg gaggttgtca
     2641 ggttgctgtg gatgtaagga taggaggtgc ccagtgctcc gctcaaggaa ggctggatct
     2701 gggccccacc tacagagagg gctcagggct ggaccggggg cattgtgtgc ttgggccgac
     2761 ccgggccggt ggcagacgct gttctctgtc gggagatttg cgtccccagg accctgttac
     2821 acagtgggct gttgggttgg tggctggctt ttcctctatg gacttcctct tcctgcccca
     2881 cctgcatagg cacacacacc ttgaatctgc accctctgga gggcatctgt actcctgtgc
     2941 aaaatgccca gtccagagac aaaacctcag actttgtgca cctaggtttc cttctcagca
     3001 gcggagactg ttctttgagt tgccttgaag tggaggccga gcggctgcgg gcccttcgcc
     3061 tccctgcggc tgaccttgat gtagctttaa gtcacactag actgcagagg ggtccgaggc
     3121 cagaaacccc tgtcctgcat cagactttca ttcccacgtt cttaggcttt gttactgata
     3181 cctcaaatcg gaagttttag ttctgagaaa ggcaagtcag cgttcttgaa atgcctgact
     3241 ggtagatatg caactctggc ctccagtctt ccatgaaaat aaatgctgcc tggaccccca
     3301 cccagaccac acactgacgg ccggctccgg cggtgcccac ccctcaggct ggcccggcac
     3361 ccaagactgg ccacagccag ctctgtcagc atgttgtgct cggacaagct gtttccttct
     3421 tctgaccaac ccaggtgtga cctggggatg cagagctttc tgttttgggt gttgggagaa
     3481 gcagcaggaa ggagtcgcca gatgatcaag ctcccccttt gctgtcatct gtgaatgagc
     3541 ttcgccaggt ggtgggcacc tgggagccat gcagaggctg tggtgctgag ttagactcca
     3601 ggtactttgt ggtcaaagga aatcgcctag ctccaggctg tgttaggaca gtattagcat
     3661 gaaggctgtg cgaccatcat gcctgctgat ccttgaggca ggcctggtcc agaaaactct
     3721 gggtcagtga ctgcgcaggg ccagccgcta ccaggacggc cctgaaacag gacacatctg
     3781 ttttttgtcc ctcaccctgg gcaggccgcg tcacaatcac agtcctcctc ctccccaccc
     3841 tgacgtctga gcgcagggct tgaattgtta gtcccaactc tggccaaaga tacttttttc
     3901 cagagacaga ggccaggagg cagtgagggg agccccgcgg ggaggcggcg gcgactgcca
     3961 cagcccttcc agcctgtctt gctggccgcc ctggttcata tttgagttta attgtactga
     4021 ccctggaccc agataagcag caactttgtg tctttggggt cacagaacat tttggggcag
     4081 tttaatgtgg taccaaactg aaaataggag ctatttatag atggagcagc acttagtgct
     4141 tcatagaaag caatgcctat ttttaaagtt acaaacgcag atatctacat agatatgctt
     4201 tgctgagaag ttaggtctgt ggtagaccag aaaccacaaa ttgacttttt ttcttagaaa
     4261 atatttctat ttgcggtaaa tatagtaata tgtaaataat gtacatctgt tgatttctgg
     4321 agtgtctgtt attcaatgat gtatatactc ccacagctcg catgaaggaa cagcctctat
     4381 tgatacttgg ttgtaaagtg aagtaagatt ggagggtgga tggctgtcag agctcttgca
     4441 gatactgtgt tcactaaata aaaatcacat gtattgttaa aa
//
