LOCUS       NM_004762               3290 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens cytohesin 1 (CYTH1), transcript variant 1, mRNA.
ACCESSION   NM_004762
VERSION     NM_004762.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 3290)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3290)
  AUTHORS   Bhatt,J.M., Hancock,W., Meissner,J.M., Kaczmarczyk,A., Lee,E.,
            Viktorova,E., Ramanadham,S., Belov,G.A. and Sztul,E.
  TITLE     Promiscuity of the catalytic Sec7 domain within the guanine
            nucleotide exchange factor GBF1 in ARF activation, Golgi
            homeostasis, and effector recruitment
  JOURNAL   Mol Biol Cell 30 (12), 1523-1535 (2019)
   PUBMED   30943106
  REMARK    GeneRIF: GBF1 alone maintains Golgi architecture; facilitates
            secretion; activates ADP-ribosylation factor (ARF)1, 3, 4, and 5;
            and recruits ARF effectors to Golgi membranes.
REFERENCE   5  (bases 1 to 3290)
  AUTHORS   Manzanillo,P., Mouchess,M., Ota,N., Dai,B., Ichikawa,R., Wuster,A.,
            Haley,B., Alvarado,G., Kwon,Y., Caothien,R., Roose-Girma,M.,
            Warming,S., McKenzie,B.S., Keir,M.E., Scherl,A., Ouyang,W. and
            Yi,T.
  TITLE     Inflammatory Bowel Disease Susceptibility Gene C1ORF106 Regulates
            Intestinal Epithelial Permeability
  JOURNAL   Immunohorizons 2 (5), 164-171 (2018)
   PUBMED   31022698
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3290)
  AUTHORS   Nagel,W., Zeitlmann,L., Schilcher,P., Geiger,C., Kolanus,J. and
            Kolanus,W.
  TITLE     Phosphoinositide 3-OH kinase activates the beta2 integrin adhesion
            pathway and induces membrane recruitment of cytohesin-1
  JOURNAL   J Biol Chem 273 (24), 14853-14861 (1998)
   PUBMED   9614087
REFERENCE   7  (bases 1 to 3290)
  AUTHORS   Meacci,E., Tsai,S.C., Adamik,R., Moss,J. and Vaughan,M.
  TITLE     Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for
            ADP-ribosylation factor
  JOURNAL   Proc Natl Acad Sci U S A 94 (5), 1745-1748 (1997)
   PUBMED   9050849
REFERENCE   8  (bases 1 to 3290)
  AUTHORS   Kolanus,W., Nagel,W., Schiller,B., Zeitlmann,L., Godar,S.,
            Stockinger,H. and Seed,B.
  TITLE     Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by
            cytohesin-1, a cytoplasmic regulatory molecule
  JOURNAL   Cell 86 (2), 233-242 (1996)
   PUBMED   8706128
REFERENCE   9  (bases 1 to 3290)
  AUTHORS   Dixon,B., Mansour,M. and Pohajdak,B.
  TITLE     Assignment of human B2-1 gene (D17S811E) to chromosome 17qter by
            PCR analysis of somatic cell hybrids and fluorescence in situ
            hybridization
  JOURNAL   Cytogenet Cell Genet 63 (1), 42-44 (1993)
   PUBMED   8449036
REFERENCE   10 (bases 1 to 3290)
  AUTHORS   Liu,L. and Pohajdak,B.
  TITLE     Cloning and sequencing of a human cDNA from cytolytic NK/T cells
            with homology to yeast SEC7
  JOURNAL   Biochim Biophys Acta 1132 (1), 75-78 (1992)
   PUBMED   1511013
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022966.13 and AC099804.3.
            
            On May 11, 2021 this sequence version replaced NM_004762.5.
            
            Summary: The protein encoded by this gene is a member of the PSCD
            family. Members of this family have identical structural
            organization that consists of an N-terminal coiled-coil motif, a
            central Sec7 domain, and a C-terminal pleckstrin homology (PH)
            domain. The coiled-coil motif is involved in homodimerization, the
            Sec7 domain contains guanine-nucleotide exchange protein activity,
            and the PH domain interacts with phospholipids and is responsible
            for association of PSCDs with membranes. Members of this family
            appear to mediate the regulation of protein sorting and membrane
            trafficking. This gene is highly expressed in natural killer and
            peripheral T cells, and regulates the adhesiveness of integrins at
            the plasma membrane of lymphocytes. A pseudogene of this gene has
            been defined on the X chromosome. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, May 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038385.1, M85169.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000446868.8/ ENSP00000389095.3
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                AC022966.13        89074-89145         c
            73-155              AC099804.3         102323-102405       c
            156-220             AC099804.3         100870-100934       c
            221-287             AC099804.3         95211-95277         c
            288-406             AC099804.3         94795-94913         c
            407-487             AC099804.3         94344-94424         c
            488-600             AC099804.3         93004-93116         c
            601-749             AC099804.3         91493-91641         c
            750-861             AC099804.3         90942-91053         c
            862-864             AC099804.3         88680-88682         c
            865-941             AC099804.3         85090-85166         c
            942-1013            AC099804.3         73644-73715         c
            1014-1168           AC099804.3         72863-73017         c
            1169-3290           AC099804.3         66721-68842         c
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..3290
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="cytohesin 1"
                     /db_xref="GeneID:9267"
                     /db_xref="HGNC:HGNC:9501"
                     /db_xref="MIM:182115"
     exon            1..72
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1247
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pleckstrin homology, Sec7 and coiled-coil domains 1;
                     cytoadhesin 1; homolog of secretory protein SEC7; SEC7
                     homolog B2-1; PH, SEC7 and coiled-coil domain-containing
                     protein 1"
                     /codon_start=1
                     /product="cytohesin-1 isoform 1"
                     /protein_id="NP_004753.1"
                     /db_xref="CCDS:CCDS42392.3"
                     /db_xref="GeneID:9267"
                     /db_xref="HGNC:HGNC:9501"
                     /db_xref="MIM:182115"
                     /translation="MEEDDSYVPSDLTAEERQELENIRRRKQELLADIQRLKDEIAEV
                     ANEIENLGSTEERKNMQRNKQVAMGRKKFNMDPKKGIQFLIENDLLKNTCEDIAQFLY
                     KGEGLNKTAIGDYLGERDEFNIQVLHAFVELHEFTDLNLVQALRQFLWSFRLPGEAQK
                     IDRMMEAFAQRYCQCNNGVFQSTDTCYVLSFAIIMLNTSLHNPNVKDKPTVERFIAMN
                     RGINDGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVK
                     TWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDSKKPNCFELYIPDNKDQ
                     VIKACKTEADGRVVEGNHTVYRISAPTPEEKEEWIKCIKAAISRDPFYEMLAARKKKV
                     SSTKRH"
     exon            73..155
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            156..220
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            221..287
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            288..406
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            407..487
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            488..600
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            601..749
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            750..861
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            862..864
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            865..941
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1013
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1168
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..3290
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3265..3270
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="hexamer: AATAAA"
     polyA_site      3290
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="major polyA site"
ORIGIN      
        1 gcggcgggac gcgagcggcg agccggagcg cggagcccgg ctcccgcacc atggaggagg
       61 acgacagcta cgttcccagt gacctgacag cagaggagcg tcaagaactg gagaacatcc
      121 gacggagaaa acaggagctg ctggctgaca ttcagaggct gaaggatgag atagcagaag
      181 tagctaatga aattgaaaac ctgggatcca cagaggaaag gaaaaacatg cagaggaaca
      241 aacaggtagc catgggcagg aaaaaattta atatggaccc taaaaagggg atccagttct
      301 taatagagaa cgacctcctg aagaacactt gtgaagacat tgcccagttc ttatataaag
      361 gcgaagggct caacaagaca gccatcggcg actacctagg ggagagagat gagtttaata
      421 tccaggttct tcatgcattt gtggagctgc atgagttcac tgatcttaat ctcgtccagg
      481 cactacggca gttcctgtgg agcttccggc tacccggaga ggcccagaag atcgaccgga
      541 tgatggaggc gtttgcccag cgatattgtc agtgcaataa tggcgtgttc cagtccacgg
      601 atacttgtta cgtcctctcc tttgccatca tcatgttgaa caccagtctg cacaacccca
      661 atgtcaaaga taagcccact gtggagaggt tcattgccat gaaccgaggc atcaatgatg
      721 ggggagacct gccggaggag ctcctccgga atctctatga gagcataaaa aatgaaccct
      781 ttaaaatccc agaagacgac gggaatgacc tcactcacac tttcttcaat ccagaccgag
      841 aaggctggct attgaaactc ggaggtggca gggtaaagac ttggaagaga cgctggttca
      901 ttctgactga caactgcctt tactactttg agtataccac ggataaggag ccccgtggaa
      961 tcatcccttt agagaatctg agtatccggg aagtggagga ctccaaaaaa ccaaactgct
     1021 ttgagcttta tatccccgac aataaagacc aagttatcaa ggcctgcaag accgaggctg
     1081 acgggcgggt ggtggagggg aaccacactg tttaccggat ctcagctccg acgcccgagg
     1141 agaaggagga gtggattaag tgcattaaag cagccatcag cagggaccct ttctacgaaa
     1201 tgctcgcagc acggaaaaag aaggtctcct ccacgaagcg acactgagcg tgcagccaag
     1261 ggcgttggtc tgcgggggcc ttggagctcc tgctcttctc ccgcacctcc atggatgcac
     1321 tgctgccgag cagagcgtcc tctgccaggc cccgccctgg attcctagag actagcttca
     1381 gcttttgcta ttttttttaa gtgggagaag ggtgggcagt tatcactggg gaagagagga
     1441 ccggccacct gtccagcatg ggctccagag ccttcctctc tcacagggca gagctcttgt
     1501 cggcagggca gcctcctggc cagtttctct gctcagtgtt ctggtagcag agctcagagc
     1561 caactgttta cctcttggtt gtccccgtga agaagccttc aaaccctgca ccataaatac
     1621 atgtgtccat atattattat atgttaagag aaaaaggtgg aaaggaagag aagccacata
     1681 ctataaagat ctattttttt ttttttaaga gagaacgtag ggctgttcag gtgcattctg
     1741 ccctggctgc gctggggagc ttctccctgg agaagagcac ctggggctgc ggccaagggg
     1801 catcagcctg ggcccgcggc agggcctggc ctgcctctcc tgtgctgtgg gagctcgctg
     1861 cctggtgctt gtctgggcga gatggacagg tgaggtcgag gacgcagagg gcagaggccc
     1921 agtggagcct cagacggcac agtcagagtc gggggcctgc cctggccggg gtcgcagtcg
     1981 gcagcagcgt gcagtccggc atctcccgcg gatgcttttc catcccaagt gcctgcggag
     2041 cgccgaggag aggagagagc tgactggacg cttacgttat tttcctcctt cagaatccaa
     2101 gttcttgttg ggctttaaag tagaaagtca gcattttcct tgagctaaat acctaataac
     2161 caaaactgtg aggaaggtta tcgggacaga ggttccggat aacctgtttc attttgggtt
     2221 ttcttcctct tccccagact ccagtcctcg ttctagagga aggagtagga cttccccgat
     2281 ccccgtaggg cttcagcttt ttctgcctca aaaccagccc taactggact actctggatg
     2341 cattttgtgg tgggccccct agaggggaag atgggccttt atctgctccg tggggtgcac
     2401 tggagtgagg ggggtggccg ggctgcctct cgcatctctg tcttcccctg caggcgctgt
     2461 gtgagctggc cctgcccctc ctcattacag tatgaaggga gccgtgacac gcagcatttt
     2521 cctgccgttc tctcagggac tctcagggca gctcctgcca ctccgccagg gccagcatgc
     2581 cagtccaggc agagcaggtg gctggctgtc tggccgtctc gccccgcccc tccacaggac
     2641 cctggaccag ggcggtgcag ggcgcagccc tgaggaggca ggtggaggag ctgcgggttt
     2701 tcacagggcc gcgtcgccac ggctcctctg atcctttagg gttggcgagc atctctggaa
     2761 atagcttttg cagaggagtg gtgggaggaa tagaggggga cagtctgtca cctccctccc
     2821 cgccactttg tgtagatcct acctggaggg aatggcttta ggcacttttg tgccagagct
     2881 tgtgagggtg acagaagagg gtccaggctg gaaacctgaa ctttctgggt gggagaacca
     2941 ggtggtgcct gccgaggtct gggcgtgttt gggccggtgc tggagcctgt ccagctggcc
     3001 cgggccctgg cctggttctc aagtgtttcc tagacagaga ggcacctggg tcagtattag
     3061 tctatttatc agaggtgtaa ataatctatg tatagttttt ctccttttag attattttgt
     3121 atttgtttaa aagaagtttt gtcaaaatac aaaaatataa agaaatgact gaaagttgtt
     3181 gacagggttt ttaagaaata attattctaa ttgtttttgt ttgtttgttt ttgccttgta
     3241 aactagcgcc aaggaactgc agcaaataaa ctccaactct gcccaagcca
//
