LOCUS       NM_001394678            7589 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens cytohesin 1 (CYTH1), transcript variant 12, mRNA.
ACCESSION   NM_001394678
VERSION     NM_001394678.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 7589)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 7589)
  AUTHORS   Bhatt,J.M., Hancock,W., Meissner,J.M., Kaczmarczyk,A., Lee,E.,
            Viktorova,E., Ramanadham,S., Belov,G.A. and Sztul,E.
  TITLE     Promiscuity of the catalytic Sec7 domain within the guanine
            nucleotide exchange factor GBF1 in ARF activation, Golgi
            homeostasis, and effector recruitment
  JOURNAL   Mol Biol Cell 30 (12), 1523-1535 (2019)
   PUBMED   30943106
  REMARK    GeneRIF: GBF1 alone maintains Golgi architecture; facilitates
            secretion; activates ADP-ribosylation factor (ARF)1, 3, 4, and 5;
            and recruits ARF effectors to Golgi membranes.
REFERENCE   5  (bases 1 to 7589)
  AUTHORS   Manzanillo,P., Mouchess,M., Ota,N., Dai,B., Ichikawa,R., Wuster,A.,
            Haley,B., Alvarado,G., Kwon,Y., Caothien,R., Roose-Girma,M.,
            Warming,S., McKenzie,B.S., Keir,M.E., Scherl,A., Ouyang,W. and
            Yi,T.
  TITLE     Inflammatory Bowel Disease Susceptibility Gene C1ORF106 Regulates
            Intestinal Epithelial Permeability
  JOURNAL   Immunohorizons 2 (5), 164-171 (2018)
   PUBMED   31022698
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7589)
  AUTHORS   Nagel,W., Zeitlmann,L., Schilcher,P., Geiger,C., Kolanus,J. and
            Kolanus,W.
  TITLE     Phosphoinositide 3-OH kinase activates the beta2 integrin adhesion
            pathway and induces membrane recruitment of cytohesin-1
  JOURNAL   J Biol Chem 273 (24), 14853-14861 (1998)
   PUBMED   9614087
REFERENCE   7  (bases 1 to 7589)
  AUTHORS   Meacci,E., Tsai,S.C., Adamik,R., Moss,J. and Vaughan,M.
  TITLE     Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for
            ADP-ribosylation factor
  JOURNAL   Proc Natl Acad Sci U S A 94 (5), 1745-1748 (1997)
   PUBMED   9050849
REFERENCE   8  (bases 1 to 7589)
  AUTHORS   Kolanus,W., Nagel,W., Schiller,B., Zeitlmann,L., Godar,S.,
            Stockinger,H. and Seed,B.
  TITLE     Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by
            cytohesin-1, a cytoplasmic regulatory molecule
  JOURNAL   Cell 86 (2), 233-242 (1996)
   PUBMED   8706128
REFERENCE   9  (bases 1 to 7589)
  AUTHORS   Dixon,B., Mansour,M. and Pohajdak,B.
  TITLE     Assignment of human B2-1 gene (D17S811E) to chromosome 17qter by
            PCR analysis of somatic cell hybrids and fluorescence in situ
            hybridization
  JOURNAL   Cytogenet Cell Genet 63 (1), 42-44 (1993)
   PUBMED   8449036
REFERENCE   10 (bases 1 to 7589)
  AUTHORS   Liu,L. and Pohajdak,B.
  TITLE     Cloning and sequencing of a human cDNA from cytolytic NK/T cells
            with homology to yeast SEC7
  JOURNAL   Biochim Biophys Acta 1132 (1), 75-78 (1992)
   PUBMED   1511013
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC099804.3.
            
            Summary: The protein encoded by this gene is a member of the PSCD
            family. Members of this family have identical structural
            organization that consists of an N-terminal coiled-coil motif, a
            central Sec7 domain, and a C-terminal pleckstrin homology (PH)
            domain. The coiled-coil motif is involved in homodimerization, the
            Sec7 domain contains guanine-nucleotide exchange protein activity,
            and the PH domain interacts with phospholipids and is responsible
            for association of PSCDs with membranes. Members of this family
            appear to mediate the regulation of protein sorting and membrane
            trafficking. This gene is highly expressed in natural killer and
            peripheral T cells, and regulates the adhesiveness of integrins at
            the plasma membrane of lymphocytes. A pseudogene of this gene has
            been defined on the X chromosome. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, May 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.1666962.1,
                                           SRR14038196.3247192.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-354               AC099804.3         115886-116239       c
            355-437             AC099804.3         102323-102405       c
            438-502             AC099804.3         100870-100934       c
            503-569             AC099804.3         95211-95277         c
            570-688             AC099804.3         94795-94913         c
            689-769             AC099804.3         94344-94424         c
            770-882             AC099804.3         93004-93116         c
            883-1031            AC099804.3         91493-91641         c
            1032-1143           AC099804.3         90942-91053         c
            1144-1220           AC099804.3         85090-85166         c
            1221-1292           AC099804.3         73644-73715         c
            1293-7589           AC099804.3         66721-73017         c
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..7589
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="cytohesin 1"
                     /db_xref="GeneID:9267"
                     /db_xref="HGNC:HGNC:9501"
                     /db_xref="MIM:182115"
     exon            1..354
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            355..437
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            438..502
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            503..569
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     CDS             510..1451
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="isoform 8 is encoded by transcript variant 12;
                     pleckstrin homology, Sec7 and coiled-coil domains 1;
                     cytoadhesin 1; homolog of secretory protein SEC7; SEC7
                     homolog B2-1; PH, SEC7 and coiled-coil domain-containing
                     protein 1"
                     /codon_start=1
                     /product="cytohesin-1 isoform 8"
                     /protein_id="NP_001381607.1"
                     /db_xref="GeneID:9267"
                     /db_xref="HGNC:HGNC:9501"
                     /db_xref="MIM:182115"
                     /translation="MQRNKQVAMGRKKFNMDPKKGIQFLIENDLLKNTCEDIAQFLYK
                     GEGLNKTAIGDYLGERDEFNIQVLHAFVELHEFTDLNLVQALRQFLWSFRLPGEAQKI
                     DRMMEAFAQRYCQCNNGVFQSTDTCYVLSFAIIMLNTSLHNPNVKDKPTVERFIAMNR
                     GINDGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTW
                     KRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDSKKPNCFELYIPDNKDQVI
                     KACKTEADGRVVEGNHTVYRISAPTPEEKEEWIKCIK"
     exon            570..688
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            689..769
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            770..882
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1031
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1143
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1220
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1292
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..7589
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7564..7569
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="hexamer: AATAAA"
     polyA_site      7589
                     /gene="CYTH1"
                     /gene_synonym="B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7"
                     /note="major polyA site"
ORIGIN      
        1 acacactcgg agcgtctgct agggctgcag ctctgcgcgt ccatctctgc gctctgcgcc
       61 agccttcccc accgggccca gcgagtttcc acctgcaggg ccggtgctct cagcttttgt
      121 tttctctgga caccttcttc tctacccctg ctgtgctggc gcacacatgc tgtccttgct
      181 ccggctctcc ctgcacctac agctgtggga gctcccggca gcctggtact gtggggtcgc
      241 tgcacggagc caccatggcc tctgacgtgg agagctgtga cctgccgagg ctttgatgtt
      301 gctgcccagg gctggccagg acactggggc cgcaggagag gggactcacc gtggttccca
      361 gtgacctgac agcagaggag cgtcaagaac tggagaacat ccgacggaga aaacaggagc
      421 tgctggctga cattcagagg ctgaaggatg agatagcaga agtagctaat gaaattgaaa
      481 acctgggatc cacagaggaa aggaaaaaca tgcagaggaa caaacaggta gccatgggca
      541 ggaaaaaatt taatatggac cctaaaaagg ggatccagtt cttaatagag aacgacctcc
      601 tgaagaacac ttgtgaagac attgcccagt tcttatataa aggcgaaggg ctcaacaaga
      661 cagccatcgg cgactaccta ggggagagag atgagtttaa tatccaggtt cttcatgcat
      721 ttgtggagct gcatgagttc actgatctta atctcgtcca ggcactacgg cagttcctgt
      781 ggagcttccg gctacccgga gaggcccaga agatcgaccg gatgatggag gcgtttgccc
      841 agcgatattg tcagtgcaat aatggcgtgt tccagtccac ggatacttgt tacgtcctct
      901 cctttgccat catcatgttg aacaccagtc tgcacaaccc caatgtcaaa gataagccca
      961 ctgtggagag gttcattgcc atgaaccgag gcatcaatga tgggggagac ctgccggagg
     1021 agctcctccg gaatctctat gagagcataa aaaatgaacc ctttaaaatc ccagaagacg
     1081 acgggaatga cctcactcac actttcttca atccagaccg agaaggctgg ctattgaaac
     1141 tcggtggcag ggtaaagact tggaagagac gctggttcat tctgactgac aactgccttt
     1201 actactttga gtataccacg gataaggagc cccgtggaat catcccttta gagaatctga
     1261 gtatccggga agtggaggac tccaaaaaac caaactgctt tgagctttat atccccgaca
     1321 ataaagacca agttatcaag gcctgcaaga ccgaggctga cgggcgggtg gtggagggga
     1381 accacactgt ttaccggatc tcagctccga cgcccgagga gaaggaggag tggattaagt
     1441 gcattaagta agtgactgct gagaaagggc agtgcgcgcc tggtgcagcc ctcacctcac
     1501 caggtgttga tcatcttagt ggttaaacat gccgcccgca tctcttctgt gctccaagtt
     1561 cttagggaag cttcgtcctg gttcccagct gacggcttcc caggaggcct ttggattctt
     1621 ggctattata gcatatgtaa gagagcacgt cccatctggg ttccccacgt gtcctcatag
     1681 acccaaaata ggaagaggcg agtgagctac ccacacagat gccatctgct caactggtct
     1741 ttctgtgtct gacgaagccg ctctcattct catagtgaca gctcagagga gatagtagtc
     1801 atgttctgta atttgtgaat cctgtgagac accattttcc tagctacaga tgaggaaact
     1861 gaggcccata gaggttaaga aatttgctgg aagtcacata ccgtgatcca aggtccggga
     1921 ggagggagaa gcttcctcag ggttccacag ctaagaagtg gagctcccaa gtccaggcct
     1981 ctgactccta cctctcggct ctgttggctc cactgggcca cgccatgtaa ggtctatcac
     2041 ggggctggaa atttcctcat gtgacaagta tctggaaaca tggctttcta aagtcagttg
     2101 gcttctcttg cttaatgttc ttccctggga cttggtctgc ctggctctcc ctccagtccc
     2161 tgtgcgaggg tcagcagtga gcattctgac tttgccttct ctcagtgatg ctttctcctc
     2221 cctccagtcc ctgtgcgagg gtcagcagtg agcattctga ctttgccttc tctcagtgat
     2281 cctttctcct aagagcacag ccttgccctc tccctcctgg tgcttcatcc atgagagttc
     2341 acctagctgt tttcctgaga aagactcctc agctgcctgt gtgacttcac ctggaagaca
     2401 gggagaatgt gcccacaggt gactgcgaag gtccctgtaa gtcctctgtg tactgtgtga
     2461 cgggctttct cccacccagg ctacagcccc gttcccgagg gtgtgcaata tcctctagcc
     2521 tttgttttat cttttggtat ttctactcag accagtgtag gttttggccc aagctaaata
     2581 ccttaaaact gactgaagat ggagatgatg tgatgatgga gggaaaggaa tagatgattt
     2641 atcctctcaa aagtaatctg aaaattcatg caatgaaaac agacaggaac cagctggaga
     2701 gaggatggat ttggcgccag gtcacgctgc gctgagactc ctcgctctgt ctctctgagc
     2761 gcagtgcctg gtaggtgggt aacctacctc ttctgtgtga tagaggtcat ttctgcctca
     2821 tggggctctt ttgaggatta aatgagattg tgtagtgggc atggggtttg gcagcttccc
     2881 tggcatgaca gccatcatgg gaagtgtggc cctgagcggg ggccagccca ccctccattg
     2941 agcagacgcg gtgccacggt gttttcagag tcccctacca gtaatggcac cagccctgtg
     3001 ttctggggaa gaaaacagca gtgttgtggg ggaccggtat cctttttttt aacatactga
     3061 aaaaaaaatt tattgaatca ttatgaaatg tattttttat ataagatttg aagtaagggt
     3121 ttaagtttgt tctcatgaat gaatagctga tggtccggac accatttatg gattcattgg
     3181 catcgccccc actgacttca tatgctctcc ttagagtgta cccagctccc ccaccacgct
     3241 tcatgcacct gttgctccac cgcgcctccc agcgtgagcg ccgcccgccc ctccccctgt
     3301 ggcatcgtgg tgtcctttga ctgtgctggc tggcgagttc cctgtcctcc ctcactggtc
     3361 tttcaaaaca gttttggctg gttttgtaca tgtgtctttc atatggtttt tacttctata
     3421 gtcagaaaaa ctaagcattt ttcaaagtca catttagaat cattcaatcc tttcatgagc
     3481 gtgagtgcca agttctcctg tgggcttgct ggactcctct gctctgtccc ggagcgggct
     3541 ggcctcagcc tcgggatctg cagacacccc ctttggcact ctgcaggcac actgtcctca
     3601 aaccttcttg cacccagtgc gaggtgggac cattaggatt atccccattt cacagatgag
     3661 ggacactgag gcacagcaaa gttgagtaag ttgtccaaaa ccccacagag gtggagctgg
     3721 tcctcaaacc tgagcagtgt gactcatgca gctgcactgg taaccaccgt gcagcctcag
     3781 ctgtccttgg ccctagatac tcctcccagt ggaaacattg gtgacaacag gagcaggaag
     3841 atgatctggc tggtggatgc ctcctcccca gtattgccag aaaggctttc gaggtcaagt
     3901 tcaggacgtg ttttcctctc acgaagtgct tttcctggag ttcccagcac cctcagttct
     3961 agtgcccctg cgtgtgggtg gtcccagcat tcggttctgt agaatcaggt gtgttctcta
     4021 atgctgggac tttcttcacg ctgtacccag aggtacagca gtagaactgc gtgtcagcgg
     4081 taacagcggc tgccatcgag tgtgtcggct aggcgctgag atgtgctctt tgaatatggg
     4141 atctcttttt gactctccgg aacccaggga ggtggaggtg gggcatctcc agcttgcatg
     4201 tgaggaagcg ggagttgaga agcagcaggc agggccaggc agggccgggc ctggggcctg
     4261 gtgtccgacc gcagcccagc ccccctcatc gcctgacact ctgcctctgc acacagggca
     4321 gtgccacacg cacctctctg cagaaccccc cagcttaccc gaaagggttg gcctacccag
     4381 gaagccaagg gagattcacc ccaacacctc caaacatgaa agcaggtgtc ccggccgcca
     4441 gattccctcg tgaaagcact tcaggtggtc agaccgcttc ccagtgagat cccatcggga
     4501 catgtttcta gtgctcttca gttcctagca ttccccgggg agctgcggaa gcattttctc
     4561 atggacacac tgtctcttgt gaataggttc caggtcagcc caggagagcc atagcagctg
     4621 ctggtgccac cgttcagcag gggtgagtgc cctgcctgca gtcaggaggc ttgtgcccga
     4681 gctctggaac aaatcatcac ttaggataca gcttccctgg aaagaaatta agtgtcagga
     4741 cttttagacc ataagttgct tgaaagtcga gaatggcaga catagggttg tggtgttgcc
     4801 agtccactgc aggtgctcca gcccgcggcg cggcctgcgc tgctgtcttt gaggctgtag
     4861 cacaagcatg agctcgggcc ccctccctgt gcaccggaga cccagccagg tccagccggt
     4921 ctgtccatgg tgccccacca gcagcatcgt gctgggcagt gccgcctgca gagtcatgga
     4981 gccttagtta ctgagcaggt gcacgtgggg ggcttggaag gccccactgc attaccatgc
     5041 cagctatcac acaccccgtg ccagaggact gcatgtgaca cggcttgatt acgtggcact
     5101 cgctgctgca aagcaaagtc agatgtcatc atggaaactc aagcaccagt ctttttctct
     5161 gaattggaat atagctgtaa gaatgtggta tgattctgtt cctaaatgtg aattgattat
     5221 tatgttgaaa caggtaaaaa ccccaaaatt ttcttgtcac gtgttcctgt gtctctttcg
     5281 aagtgtgtca ccttaggtca ctgtgtggac acagcaaggg tggaggacgc taacttggcc
     5341 tttgcagtga tggtggggtg ggacaggtgt tctggggcac gaggggcccc tgagaatccc
     5401 ctgcctgggt gtgtttcttc tgattctgtc cctcacgtct ctgttttctc ccttttctgt
     5461 gctccagagc agccatcagc agggaccctt tctacgaaat gctcgcagca cggaaaaaga
     5521 aggtctcctc cacgaagcga cactgagcgt gcagccaagg gcgttggtct gcgggggcct
     5581 tggagctcct gctcttctcc cgcacctcca tggatgcact gctgccgagc agagcgtcct
     5641 ctgccaggcc ccgccctgga ttcctagaga ctagcttcag cttttgctat tttttttaag
     5701 tgggagaagg gtgggcagtt atcactgggg aagagaggac cggccacctg tccagcatgg
     5761 gctccagagc cttcctctct cacagggcag agctcttgtc ggcagggcag cctcctggcc
     5821 agtttctctg ctcagtgttc tggtagcaga gctcagagcc aactgtttac ctcttggttg
     5881 tccccgtgaa gaagccttca aaccctgcac cataaataca tgtgtccata tattattata
     5941 tgttaagaga aaaaggtgga aaggaagaga agccacatac tataaagatc tatttttttt
     6001 tttttaagag agaacgtagg gctgttcagg tgcattctgc cctggctgcg ctggggagct
     6061 tctccctgga gaagagcacc tggggctgcg gccaaggggc atcagcctgg gcccgcggca
     6121 gggcctggcc tgcctctcct gtgctgtggg agctcgctgc ctggtgcttg tctgggcgag
     6181 atggacaggt gaggtcgagg acgcagaggg cagaggccca gtggagcctc agacggcaca
     6241 gtcagagtcg ggggcctgcc ctggccgggg tcgcagtcgg cagcagcgtg cagtccggca
     6301 tctcccgcgg atgcttttcc atcccaagtg cctgcggagc gccgaggaga ggagagagct
     6361 gactggacgc ttacgttatt ttcctccttc agaatccaag ttcttgttgg gctttaaagt
     6421 agaaagtcag cattttcctt gagctaaata cctaataacc aaaactgtga ggaaggttat
     6481 cgggacagag gttccggata acctgtttca ttttgggttt tcttcctctt ccccagactc
     6541 cagtcctcgt tctagaggaa ggagtaggac ttccccgatc cccgtagggc ttcagctttt
     6601 tctgcctcaa aaccagccct aactggacta ctctggatgc attttgtggt gggcccccta
     6661 gaggggaaga tgggccttta tctgctccgt ggggtgcact ggagtgaggg gggtggccgg
     6721 gctgcctctc gcatctctgt cttcccctgc aggcgctgtg tgagctggcc ctgcccctcc
     6781 tcattacagt atgaagggag ccgtgacacg cagcattttc ctgccgttct ctcagggact
     6841 ctcagggcag ctcctgccac tccgccaggg ccagcatgcc agtccaggca gagcaggtgg
     6901 ctggctgtct ggccgtctcg ccccgcccct ccacaggacc ctggaccagg gcggtgcagg
     6961 gcgcagccct gaggaggcag gtggaggagc tgcgggtttt cacagggccg cgtcgccacg
     7021 gctcctctga tcctttaggg ttggcgagca tctctggaaa tagcttttgc agaggagtgg
     7081 tgggaggaat agagggggac agtctgtcac ctccctcccc gccactttgt gtagatccta
     7141 cctggaggga atggctttag gcacttttgt gccagagctt gtgagggtga cagaagaggg
     7201 tccaggctgg aaacctgaac tttctgggtg ggagaaccag gtggtgcctg ccgaggtctg
     7261 ggcgtgtttg ggccggtgct ggagcctgtc cagctggccc gggccctggc ctggttctca
     7321 agtgtttcct agacagagag gcacctgggt cagtattagt ctatttatca gaggtgtaaa
     7381 taatctatgt atagtttttc tccttttaga ttattttgta tttgtttaaa agaagttttg
     7441 tcaaaataca aaaatataaa gaaatgactg aaagttgttg acagggtttt taagaaataa
     7501 ttattctaat tgtttttgtt tgtttgtttt tgccttgtaa actagcgcca aggaactgca
     7561 gcaaataaac tccaactctg cccaagcca
//
