LOCUS       NM_004820               7462 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens cytochrome P450 family 7 subfamily B member 1
            (CYP7B1), transcript variant 1, mRNA.
ACCESSION   NM_004820
VERSION     NM_004820.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Zhang,Z., Su,Z., Li,Z., Li,J., Yu,W., Ye,G., Lin,J., Che,Y., Xu,P.,
            Zeng,Y., Wu,Y., Shen,H. and Xie,Z.
  TITLE     CYP7B1-mediated 25-hydroxycholesterol degradation maintains
            quiescence-activation balance and improves therapeutic potential of
            mesenchymal stem cells
  JOURNAL   Cell Chem Biol 31 (7), 1277-1289 (2024)
   PUBMED   38382532
  REMARK    GeneRIF: CYP7B1-mediated 25-hydroxycholesterol degradation
            maintains quiescence-activation balance and improves therapeutic
            potential of mesenchymal stem cells.
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Theuriet,J., Pegat,A., Leblanc,P., Vukusic,S., Cazeneuve,C.,
            Millecamps,S., Banneau,G., Guillaud-Bataille,M. and Bernard,E.
  TITLE     Phenoconversion from Spastic Paraplegia to ALS/FTD Associated with
            CYP7B1 Compound Heterozygous Mutations
  JOURNAL   Genes (Basel) 12 (12), 1876 (2021)
   PUBMED   34946825
  REMARK    GeneRIF: Phenoconversion from Spastic Paraplegia to ALS/FTD
            Associated with CYP7B1 Compound Heterozygous Mutations.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7462)
  AUTHORS   Liang,T., Zhang,X., Liang,A., Wu,H., Wang,Q., He,J., Long,M. and
            Jin,T.
  TITLE     The effect of CYP7B1 polymorphisms on the risk of coronary heart
            disease in Hainan Han population
  JOURNAL   BMC Med Genomics 14 (1), 220 (2021)
   PUBMED   34493281
  REMARK    GeneRIF: The effect of CYP7B1 polymorphisms on the risk of coronary
            heart disease in Hainan Han population.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7462)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 7462)
  AUTHORS   Nelson,D.R., Zeldin,D.C., Hoffman,S.M., Maltais,L.J., Wain,H.M. and
            Nebert,D.W.
  TITLE     Comparison of cytochrome P450 (CYP) genes from the mouse and human
            genomes, including nomenclature recommendations for genes,
            pseudogenes and alternative-splice variants
  JOURNAL   Pharmacogenetics 14 (1), 1-18 (2004)
   PUBMED   15128046
  REMARK    Review article
REFERENCE   6  (bases 1 to 7462)
  AUTHORS   Setchell,K.D., Schwarz,M., O'Connell,N.C., Lund,E.G., Davis,D.L.,
            Lathe,R., Thompson,H.R., Weslie Tyson,R., Sokol,R.J. and
            Russell,D.W.
  TITLE     Identification of a new inborn error in bile acid synthesis:
            mutation of the oxysterol 7alpha-hydroxylase gene causes severe
            neonatal liver disease
  JOURNAL   J Clin Invest 102 (9), 1690-1703 (1998)
   PUBMED   9802883
REFERENCE   7  (bases 1 to 7462)
  AUTHORS   Schwarz,M., Lund,E.G. and Russell,D.W.
  TITLE     Two 7 alpha-hydroxylase enzymes in bile acid biosynthesis
  JOURNAL   Curr Opin Lipidol 9 (2), 113-118 (1998)
   PUBMED   9559267
  REMARK    Review article
REFERENCE   8  (bases 1 to 7462)
  AUTHORS   Hentati,A., Pericak-Vance,M.A., Hung,W.Y., Belal,S., Laing,N.,
            Boustany,R.M., Hentati,F., Ben Hamida,M. and Siddique,T.
  TITLE     Linkage of 'pure' autosomal recessive familial spastic paraplegia
            to chromosome 8 markers and evidence of genetic locus heterogeneity
  JOURNAL   Hum Mol Genet 3 (8), 1263-1267 (1994)
   PUBMED   7987300
REFERENCE   9  (bases 1 to 7462)
  AUTHORS   Amendola,M. and Squires,J.E.
  TITLE     Pediatric Genetic Cholestatic Liver Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36108118
REFERENCE   10 (bases 1 to 7462)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104232.5, BC029155.1,
            BP349825.1, AC090136.6 and BQ575990.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004820.4.
            
            Summary: This gene encodes a member of the cytochrome P450
            superfamily of enzymes. The cytochrome P450 proteins are
            monooxygenases which catalyze many reactions involved in drug
            metabolism and synthesis of cholesterol, steroids and other lipids.
            This endoplasmic reticulum membrane protein catalyzes the first
            reaction in the cholesterol catabolic pathway of extrahepatic
            tissues, which converts cholesterol to bile acids. This enzyme
            likely plays a minor role in total bile acid synthesis, but may
            also be involved in the development of atherosclerosis,
            neurosteroid metabolism and sex hormone synthesis. Mutations in
            this gene have been associated with hereditary spastic paraplegia
            (SPG5 or HSP), an autosomal recessive disorder. [provided by
            RefSeq, Apr 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.34537.1,
                                           SRR1660807.27723.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000310193.4/ ENSP00000310721.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AC104232.5         48632-48650
            20-1543             BC029155.1         1-1524
            1544-1841           BP349825.1         290-587
            1842-6778           AC090136.6         1513-6449
            6779-7460           BQ575990.1         18-699              c
            7461-7462           AC090136.6         7132-7133
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q12.3"
     gene            1..7462
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /note="cytochrome P450 family 7 subfamily B member 1"
                     /db_xref="GeneID:9420"
                     /db_xref="HGNC:HGNC:2652"
                     /db_xref="MIM:603711"
     exon            1..272
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..1671
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /EC_number="1.14.14.29"
                     /EC_number="1.14.14.26"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cytochrome P450, subfamily VIIB (oxysterol 7
                     alpha-hydroxylase), polypeptide 1; 25-hydroxycholesterol
                     7-alpha-hydroxylase; cytochrome P450 7B1; oxysterol
                     7-alpha-hydroxylase; 3-hydroxysteroid 7-alpha hydroxylase;
                     24-hydroxycholesterol 7-alpha-hydroxylase;
                     25/26-hydroxycholesterol 7-alpha-hydroxylase"
                     /codon_start=1
                     /product="cytochrome P450 7B1 isoform 1"
                     /protein_id="NP_004811.1"
                     /db_xref="CCDS:CCDS6180.1"
                     /db_xref="GeneID:9420"
                     /db_xref="HGNC:HGNC:2652"
                     /db_xref="MIM:603711"
                     /translation="MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPG
                     EPPLIKGWLPYLGVVLNLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLV
                     IKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQN
                     LKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLK
                     FDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYV
                     HEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKG
                     SGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDL
                     VAIFPPVLHGDPEIFEAPEEFRYDRFIEDGKKKTTFFKRGKKLKCYLMPFGTGTSKCP
                     GRFFALMEIKQLLVILLTYFDLEIIDDKPIGLNYSRLLFGIQYPDSDVLFRYKVKS"
     exon            273..409
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /inference="alignment:Splign:2.1.0"
     exon            410..1000
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1207
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1383
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..7462
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7441..7446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /note="hexamer: AATAAA"
     polyA_site      7462
                     /gene="CYP7B1"
                     /gene_synonym="CBAS3; CP7B; SPG5A"
                     /note="major polyA site"
ORIGIN      
        1 gacattcctg tctgcgctgc gggcaggcca gcctctccgg ccctggacta gggggcgccg
       61 ccgagagaag ccgccgcagg ctgcagagtc gccgaccgcg ctgttcgcag gcagaccccg
      121 ggctgcccac cgcggcctag cgcgcgccgg atggcaggag aagtgtccgc ggccacgggc
      181 cgcttttcgc tggagcggtt gggcctcccg ggcctggccc tcgccgcggc cctgctgctc
      241 ctggccctct gcttgcttgt ccggcgcacc aggagacccg gtgagcctcc attgataaaa
      301 ggttggcttc cttatcttgg agtggtcctg aacttacgaa aagacccctt aaggttcatg
      361 aaaacacttc aaaagcaaca tggtgacact ttcacagttc ttcttggtgg aaagtacata
      421 acatttatcc tggacccctt ccagtaccag ctagtgataa aaaatcataa acaattaagc
      481 tttcgagtat tttctaataa attattagag aaagcattta gcatcagtca gttgcaaaaa
      541 aatcatgaca tgaatgatga gcttcacctc tgctatcaat ttttgcaagg caaatctttg
      601 gacatactct tggaaagcat gatgcagaat ctaaaacaag tttttgaacc ccagctgtta
      661 aaaaccacaa gttgggacac ggcagaactg tatccattct gcagctcaat aatatttgag
      721 atcacattta caactatata tggaaaagtt attgtttgtg acaacaacaa atttattagt
      781 gagctaagag atgatttttt aaaatttgat gacaagtttg catatttagt atccaacata
      841 cccattgagc ttctaggaaa tgtcaagtct attagagaga aaattataaa atgcttctca
      901 tcagaaaagt tagccaagat gcaaggatgg tcagaagttt ttcaaagcag gcaagatgtc
      961 ctggagaaat attatgtgca cgaggacctt gaaataggag cacatcattt aggctttctc
     1021 tgggcctctg tggcaaacac tattccaact atgttctggg caatgtatta tcttctgcgg
     1081 cacccagaag ctatggcagc agtgcgtgac gaaattgacc gtttgctgca gtcaacaggt
     1141 caaaagaaag ggtctggatt tcccatccac ctcaccagag aacaattgga cagcctaatc
     1201 tgcctagaaa gcagcatttt tgaagcttta cgactgtcct catattcaac caccattcgt
     1261 tttgttgagg aggatttgac tctcagttca gagaccgggg actactgtgt gcgaaaggga
     1321 gacttggtag ccatctttcc tccagtccta catggtgacc ctgaaatctt tgaagctcca
     1381 gaggagttta gatatgatcg ttttatagaa gatggtaaga agaaaaccac ctttttcaaa
     1441 agagggaaaa agctgaagtg ttacctaatg ccgtttggaa ctggaaccag caaatgtcca
     1501 ggccgatttt ttgcacttat ggaaataaaa caattgttgg ttatactttt aacttatttt
     1561 gatttagaaa taattgatga taagcccata ggactaaact acagccgctt gttgtttggt
     1621 attcagtatc cagattctga tgttttattt agatacaaag tgaaatctta gagaagctaa
     1681 aaggaaagaa aataaatcta tcaaaattac cctaaacatc ctaagctcat ctatttcttt
     1741 ttaatttctg caaatgtaat tgatttattt gtttaaaaag cgctaatttc tatttgatct
     1801 gatatcagtc cagtttgtcc ttagtcacaa agcccatcat aatataaaac aggatggtgg
     1861 caggaaaatg gacatcaaaa tcaacttaag ggtaggctca aaacagggtt gttgttgttt
     1921 ttttagtttc tccttgttgt gattttcacc tgttataata aatgtacctt cacaccaata
     1981 gattgggcac tgtggaattt aaatcactca aattgttctc taatatgtaa aattacactt
     2041 tgaactacta gaaaattgtg ctggaaatgg acacagtcta acaaattcca aattctcaca
     2101 gaagaaggaa attgaatttc catgagttac actggatgaa aatgttccct tcaagtataa
     2161 tattaataat acctatattt ccagggtatg tgtgcattaa atgagtttca cagtagattg
     2221 aatgcttcaa cttcacttca atatttaatc atgcataaat gttccttatt actttgtata
     2281 ctatggctca atagaacaaa atttctggtt acataagacc tttcatgttc aagttagata
     2341 ctgataagat ttaatgtata agaaaacaca gaacctatta tatattcaga aattgcccta
     2401 gacattagta ttaggtttat tatgggagca aatgttgaaa taattacagc attgattata
     2461 tcatttggct ttagtataaa ctatacaggt tatgcaaata ttgcaattaa ccctttccta
     2521 aatgctttca tatatataaa ctgagaagct agatatagta tttggttttg atgtctcacc
     2581 ttttggattt ggagttcatg tgttctattt tgaattacaa catcatgtaa tgcagtgtaa
     2641 tttcatatga cacatgcagc attgtgtcaa taattcaaag attttttttc aattgagaga
     2701 gtatctggct gtgttgccca ggctggtctc aaatcctgac ctcaagtgat cctcccgctt
     2761 tggtcccttg ggaacctgga ctacaggcat gtaccaccaa gcccagtgat tcaaagattc
     2821 taatatcaga gtaccacatg attggcatct gatataaatg atgatgcaat atataagcag
     2881 gcaaggcagg taataaagtc cagttaattc atgcttcgtt aattggaact tctaatattt
     2941 cagaaatctg ctactgtttt gagaaaagga aactgacttt aaaaataaca ttacaaaata
     3001 tttacattct tagtaagtaa ataagacagt ctagcattta ttttatcctt cctgaagtac
     3061 atgttctgaa tgttccttca gtgaaggtct gctggtgcaa attctatttt tgcttgattt
     3121 agggtgtctt tatttttact ctccttcttg aaagatggtt ttgctggatg cacaaatcta
     3181 gattgacagt tatttcctca cagcactttg aagatattgc ccgttcttaa ggtatccatt
     3241 gttgttattg ggcggatagc cgttagtgag tttaattact caataggtag tctgtctctt
     3301 ctctctgact actttaacgt gtcctctttg tctttgatgt tcttagtttc tttctgtttc
     3361 tcatgcttgc atgtcctgct gcttcttgaa tctgagcaca ggtgtctccc atcaagtatg
     3421 gaatcttctc agccattctc tctgtgaata ttactgttct cacactctac attagatcct
     3481 ctcactctac atacatcctt tgatcttttc tcaccacact ttgtatctct aacttacact
     3541 ccatgtaatt tctttagatg tctcttccag tttcttcttt gaacagtata tctaatctct
     3601 ccaactcatc cactgagttt gttttttcct tctttttgtt ttaactctag gttcatcgag
     3661 ttgagtgatc actctcctta cactctttag ttcatgatac acaattataa ttggttctaa
     3721 taaggtccct ctggctttat ttacttattc tgttttgtcc ctaataccca ctttcatacc
     3781 ccttcctaat atcataggca aatattctaa tgtgtttgat gcatatcttg gtatgtatat
     3841 gttttcatgt aacatatatt atcttgagta cctattttct gtaagttata ttcttcctaa
     3901 ttttcttatg aagctctgtg attttaaaga tatattcata tttctgtagt atatacatag
     3961 tatactacta tatttaatct attgcttcta actgctgcac agcaatccat aatgtgcaca
     4021 cattatgttt taatgatcta gttacccagt gatggacact gtcagcagga cagttattgt
     4081 gggacagagg ccaaagcaga acttaaaagc ttttccatca cctatcctca cactactgtg
     4141 cctaaccatc tcagcccagg gctgctaaat gccttgtccc caagccccac cacccacccc
     4201 ccacatacac acatttagtc catgataact ttaattcttc ctaagatgtt ctaaatttgt
     4261 tatgtcccat cctcagatcc atccaatcct ctccattctg tagggtctcc aaggttgatt
     4321 ttgagtcaga gtgtcagtct ccagcttgtt aaccttcagg ttttccattc cactgagttt
     4381 gctaagttca atcattacat ttttcagttt tacgattccc ttttgttctt catatatgct
     4441 gcttattttt tataatatct tgtaccttta ttataaactc aacatccttt ttcatttctt
     4501 caactgtatg aagcaaacat tttagattct gtatctgata tttccaaatc attggtgttt
     4561 tgtggatcta attctgtagt ttatgtgttt tgctgtcact gttaccagga atggcttgtt
     4621 tactgagccc atgttccttg gatttttatc tctgaaaaat ctttagacct agatttaaag
     4681 ttcattcctc aagagagaat ttgtatttgc atttttcaga ctgctgatac tactaccaaa
     4741 ctcagatcac cttaaaattt cagcttgatt atctgggagt ggggagggaa cgctacatag
     4801 gtattgtggt ttctagccct aaatcaggta gaatgcaacc attttattag gaatagacag
     4861 gggacacatt tgggggttca tttttttgtt tatttttacc cggagctaag actgagatag
     4921 gcaaatatac tgacagtttt gctctgtgga gcacagtttc tttctagttc acctattgag
     4981 tatgttgtct ttgcaagttc ttggctttat gcaaaggcct tggattacac acacacacac
     5041 acacacacac acacacacac acacacacac acacacacac cctgggccct agcctttagt
     5101 ccaccacaaa gcatactctg tccttttcgt acttggacgt ctagttcgcc atgttgccta
     5161 aagtggaagt tccatagctt taaattcaac ttagtggtac acttttgtga tgttaaggca
     5221 cagagaacac aggaaattat tccattccaa tttcggctta ttttataact ggaaatgtgt
     5281 acaggaattt agaaatggaa agtaaggata aatgaaatgg ttgagaaaag atgacataaa
     5341 aggaatgaat agtagaacca aaacaaaaca ttgagaatct tgtgacagtc ttaaatccag
     5401 taactaaata gtatttacaa atagaaaaac atgtcctgga caaatcagtt aaaagatgca
     5461 gattctggct gtgacataac tggcttatta ttaaacaatt tccgtctcag agcttcaacg
     5521 tcctcatttg aaaaataaaa taataaggcc ctttgacatc agagttcatt gtaacgatta
     5581 aacacagtaa cgtgtatgga tttatggtat aatgcaatat accaatgcaa agtttaatga
     5641 agatacttca acagtgttgt gcctttaaaa aattgctctt gtgtattgtt caagggaatc
     5701 gttttttata gtcatttgtt aattcattgt tcatttattc aaccaaacat tcattgagca
     5761 ttttctctgt tctagatatg attgatgcta gacactggat tccccttccc cttcaagaac
     5821 atactatttg ttagggaaaa caatacgtag gcaactaatt tattataaat gcagttgtaa
     5881 gtgataaatt catctcttta aaactatttt aaaattctga tttatcacta gttctaacta
     5941 gccttccatc agtcattcca aagtaatggt ctgtaatgag aaatcactat gtataattat
     6001 acacaataaa aatatataca acaggtattt tgataatatg ataattaaaa ccaaatatag
     6061 tcattgaggc ttagaatttt taaaaactgt attatatatt gtaaaatccc atcctttttt
     6121 ttttttagat ggagtcttgc tctgtcgccc aggctggagt ccagtggcat gatctcagct
     6181 cactgcaacc tccacttcct gggttcaagc aattcccttg tctcagccct ccgagtagct
     6241 gggactacag gcacccacca ctatgcctga ccaacttttg tatttttagt ggagacgagg
     6301 tttcaccatg ttgatcaggc tggtctcgaa ctccagacct caggtgatcc acccgcctcg
     6361 gactccctaa gtgctggggt tgcagacatg agccaccgtg cccgccccca tcttttcatt
     6421 tttatagctt cacctaagtt ttgaattaaa agaaataaat aattaatacc caaaatattg
     6481 ttttatatca atgaccaacg taatgaaaca ctcagcagaa actaaagccc tgaagtggtt
     6541 aagaaaaagc tacctatcac taaatcaggc atgcttataa gcaacctaga agaaaacttt
     6601 tatctgcctt gttttggctt tcctggcata cttccttact tcatctccat tttataatta
     6661 agttttgggt cacaagtcta aggcaaagga gcttccatac tgaaaatcta catttttaat
     6721 gcttatttta tcataaaaat aatttgggta attttctgca agtgacttct aacttaacag
     6781 tagaagttta aaactgttca aagaccaaag cacaacattt atctagtgtt tgatcctagt
     6841 ataaaagaat ggcaataatt atgtgaacag gaattacatg cccttagaat gtgcattttt
     6901 taacctatta aatttgccaa tcttgcaaac tattgtttac ttgtattgca taattagata
     6961 ctcatattaa catattgaat tcagaaaaag ttagcaagcc aagatgacat tctctgtagc
     7021 actattttaa attataatga atgatcacat aaaactcttt agtatttatc taaagtaatt
     7081 attactctac ttcatttgtt tatctaaatc agtgatcatt gatgtttgaa ctttttggct
     7141 taaatgttta ttttgtttat actacttgct agagtaaaat aaatttaata catgaaaaac
     7201 tctacacaat ttaaaatagg ttataatttg tcaatactta tgttttaaaa tatttttaga
     7261 aggaggagtg ctgtatatta ttaaaacaat tttctgaaat tgtttaatat tatctttgat
     7321 tttaaaatga catatatgtg gatttacaat gaatcaaatt gtcctaaaag atgtcagata
     7381 agaaatgcaa gtgctttgca agtctaatac ttaatgttct tttatgtaca acaaaaattt
     7441 aataaattaa ctttaaagca ca
//
