LOCUS       NM_178033               2256 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens cytochrome P450 family 4 subfamily X member 1
            (CYP4X1), transcript variant 1, mRNA.
ACCESSION   NM_178033
VERSION     NM_178033.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Gao,L.P., Shi,Q., Xiao,K., Wang,J., Zhou,W., Chen,C. and Dong,X.P.
  TITLE     The genetic Creutzfeldt-Jakob disease with E200K mutation: analysis
            of clinical, genetic and laboratory features of 30 Chinese patients
  JOURNAL   Sci Rep 9 (1), 1836 (2019)
   PUBMED   30755683
  REMARK    GeneRIF: The genetic Creutzfeldt-Jakob disease with E200K mutation:
            analysis of clinical, genetic and laboratory features of 30 Chinese
            patients.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Poleggi,A., van der Lee,S., Capellari,S., Puopolo,M., Ladogana,A.,
            De Pascali,E., Lia,D., Formato,A., Bartoletti-Stella,A., Parchi,P.,
            van Duijn,C. and Pocchiari,M.
  TITLE     Age at onset of genetic (E200K) and sporadic Creutzfeldt-Jakob
            diseases is modulated by the CYP4X1 gene
  JOURNAL   J Neurol Neurosurg Psychiatry 89 (12), 1243-1249 (2018)
   PUBMED   30032116
  REMARK    GeneRIF: results indicate that the CYP4X1 gene modulates the onset
            of disease in patients with E200K genetic and sporadic
            Creutzfeldt-Jakob disease.
REFERENCE   3  (bases 1 to 2256)
  AUTHORS   Kumar,S.
  TITLE     Computational identification and binding analysis of orphan human
            cytochrome P450 4X1 enzyme with substrates
  JOURNAL   BMC Res Notes 8, 9 (2015)
   PUBMED   25595103
  REMARK    GeneRIF: Key residues were identified to be responsible for the
            binding of arachidonic acid and anandamide with orphan human
            cytochrome P450 4X1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2256)
  AUTHORS   Stark,K., Dostalek,M. and Guengerich,F.P.
  TITLE     Expression and purification of orphan cytochrome P450 4X1 and
            oxidation of anandamide
  JOURNAL   FEBS J 275 (14), 3706-3717 (2008)
   PUBMED   18549450
REFERENCE   5  (bases 1 to 2256)
  AUTHORS   Savas,U., Hsu,M.H., Griffin,K.J., Bell,D.R. and Johnson,E.F.
  TITLE     Conditional regulation of the human CYP4X1 and CYP4Z1 genes
  JOURNAL   Arch Biochem Biophys 436 (2), 377-385 (2005)
   PUBMED   15797250
  REMARK    GeneRIF: PPARalpha has a role in CYP4X1 regulation, and the
            glucocorticoid and progesterone receptors have roles in CYP4Z1 gene
            activation
REFERENCE   6  (bases 1 to 2256)
  AUTHORS   Nelson,D.R., Zeldin,D.C., Hoffman,S.M., Maltais,L.J., Wain,H.M. and
            Nebert,D.W.
  TITLE     Comparison of cytochrome P450 (CYP) genes from the mouse and human
            genomes, including nomenclature recommendations for genes,
            pseudogenes and alternative-splice variants
  JOURNAL   Pharmacogenetics 14 (1), 1-18 (2004)
   PUBMED   15128046
  REMARK    Review article
REFERENCE   7  (bases 1 to 2256)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   8  (bases 1 to 2256)
  AUTHORS   Bylund,J., Zhang,C. and Harder,D.R.
  TITLE     Identification of a novel cytochrome P450, CYP4X1, with unique
            localization specific to the brain
  JOURNAL   Biochem Biophys Res Commun 296 (3), 677-684 (2002)
   PUBMED   12176035
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC028102.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_178033.1.
            
            Summary: This gene encodes a member of the cytochrome P450
            superfamily of enzymes and is located within a cluster of genes
            belonging to this superfamily on chromosome 1. The cytochrome P450
            proteins are monooxygenases which catalyze many reactions involved
            in drug metabolism and synthesis of cholesterol, steroids and other
            lipids. The expression pattern of a similar rat protein suggests
            that this protein may be involved in neurovascular function in the
            brain. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028102.1, AY358537.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371901.4/ ENSP00000360968.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2256              BC028102.1         102-2357
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p33"
     gene            1..2256
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /note="cytochrome P450 family 4 subfamily X member 1"
                     /db_xref="GeneID:260293"
                     /db_xref="HGNC:HGNC:20244"
                     /db_xref="MIM:614999"
     exon            1..326
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1679
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /EC_number="1.14.14.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cytochrome P450 4X1; cytochrome P450, family 4, subfamily
                     X, polypeptide 1"
                     /codon_start=1
                     /product="cytochrome P450 4X1 isoform 1"
                     /protein_id="NP_828847.1"
                     /db_xref="CCDS:CCDS544.1"
                     /db_xref="GeneID:260293"
                     /db_xref="HGNC:HGNC:20244"
                     /db_xref="MIM:614999"
                     /translation="MEFSWLETRWARPFYLAFVFCLALGLLQAIKLYLRRQRLLRDLR
                     PFPAPPTHWFLGHQKFIQDDNMEKLEEIIEKYPRAFPFWIGPFQAFFCIYDPDYAKTL
                     LSRTDPKSQYLQKFSPPLLGKGLAALDGPKWFQHRRLLTPGFHFNILKAYIEVMAHSV
                     KMMLDKWEKICSTQDTSVEVYEHINSMSLDIIMKCAFSKETNCQTNSTHDPYAKAIFE
                     LSKIIFHRLYSLLYHSDIIFKLSPQGYRFQKLSRVLNQYTDTIIQERKKSLQAGVKQD
                     NTPKRKYQDFLDIVLSAKDESGSSFSDIDVHSEVSTFLLAGHDTLAASISWILYCLAL
                     NPEHQERCREEVRGILGDGSSITWDQLGEMSYTTMCIKETCRLIPAVPSISRDLSKPL
                     TFPDGCTLPAGITVVLSIWGLHHNPAVWKNPKVFDPLRFSQENSDQRHPYAYLPFSAG
                     SRNCIGQEFAMIELKVTIALILLHFRVTPDPTRPLTFPNHFILKPKNGMYLHLKKLSE
                     C"
     exon            327..468
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            469..513
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            514..641
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            642..769
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            770..924
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1031
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1222
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1356
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1421
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1504
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..2256
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2041..2046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /note="hexamer: AATAAA"
     polyA_site      2060
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
     regulatory      2233..2238
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /note="hexamer: AATAAA"
     polyA_site      2256
                     /gene="CYP4X1"
                     /gene_synonym="CYPIVX1"
                     /note="major polyA site"
ORIGIN      
        1 actgcctttc cttcttcccg cgagtcagaa gcttcgcgag ggcccagaga ggcggtgggg
       61 tgggcgaccc tacgccagct ccgggcggga gaaagcccac cctctcccgc gccccaggaa
      121 accgccggcg ttcggcgctg cgcagagcca tggaattctc ctggctggag acgcgctggg
      181 cgcggccctt ttacctggcg ttcgtgttct gcctggccct ggggctgctg caggccatta
      241 agctgtacct gcggaggcag cggctgctgc gggacctgcg ccccttccca gcgcccccca
      301 cccactggtt ccttgggcac cagaagttta ttcaggatga taacatggag aagcttgagg
      361 aaattattga aaaataccct cgtgccttcc ctttctggat tgggcccttt caggcatttt
      421 tctgtatcta tgacccagac tatgcaaaga cacttctgag cagaacagat cccaagtccc
      481 agtacctgca gaaattctca cctccacttc ttggaaaagg actagcggct ctagacggac
      541 ccaagtggtt ccagcatcgt cgcctactaa ctcctggatt ccattttaac atcctgaaag
      601 catacattga ggtgatggct cattctgtga aaatgatgct ggataagtgg gagaagattt
      661 gcagcactca ggacacaagc gtggaggtct atgagcacat caactcgatg tctctggata
      721 taatcatgaa atgcgctttc agcaaggaga ccaactgcca gacaaacagc acccatgatc
      781 cttatgcaaa agccatattt gaactcagca aaatcatatt tcaccgcttg tacagtttgt
      841 tgtatcacag tgacataatt ttcaaactca gccctcaggg ctaccgcttc cagaagttaa
      901 gccgagtgtt gaatcagtac acagatacaa taatccagga aagaaagaaa tccctccagg
      961 ctggggtaaa gcaggataac actccgaaga ggaagtacca ggattttctg gatattgtcc
     1021 tttctgccaa ggatgaaagt ggtagcagct tctcagatat tgatgtacac tctgaagtga
     1081 gcacattcct gttggcagga catgacacct tggcagcaag catctcctgg atcctttact
     1141 gcctggctct gaaccctgag catcaagaga gatgccggga ggaggtcagg ggcatcctgg
     1201 gggatgggtc ttctatcact tgggaccagc tgggtgagat gtcgtacacc acaatgtgca
     1261 tcaaggagac gtgccgattg attcctgcag tcccgtccat ttccagagat ctcagcaagc
     1321 cacttacctt cccagatgga tgcacattgc ctgcagggat caccgtggtt cttagtattt
     1381 ggggtcttca ccacaaccct gctgtctgga aaaacccaaa ggtctttgac cccttgaggt
     1441 tctctcagga gaattctgat cagagacacc cctatgccta cttaccattc tcagctggat
     1501 caaggaactg cattgggcag gagtttgcca tgattgagtt aaaggtaacc attgccttga
     1561 ttctgctcca cttcagagtg actccagacc ccaccaggcc tcttactttc cccaaccatt
     1621 ttatcctcaa gcccaagaat gggatgtatt tgcacctgaa gaaactctct gaatgttaga
     1681 tctcagggta caatgattaa acgtactttg tttttcgaag ttaaatttac agctaatgat
     1741 ccaagcagat agaaagggat caatgtatgg tgggaggatt ggaggttggt gggatagggg
     1801 tctctgtgaa gagatccaaa atcatttcta ggtacacagt gtgtcagcta gatctgtttc
     1861 tatataactt tgggagattt tcagatcttt tctgttaaac tttcactact attaatgctg
     1921 tatacaccaa tagactttca tatattttct gttgttttta aaatagtttt cagaattatg
     1981 caagtaataa gtgcatgtat gctcactgtc aaaaattccc aacactagaa aatcatgtag
     2041 aataaaaatt ttaaatctca cttcacttag ccgacattcc atgccctgac caatcctact
     2101 gcttttccta aaaacagaat aatttggtgt gcattctttc agactttttc ctatacattt
     2161 tatatgtaga aatgtagcaa tgtatttgta tagatgtgat cattcctata ttgttattga
     2221 tttttttcac ttaataaaaa ttcaccttat tcctta
//
