LOCUS       NM_000770               1924 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens cytochrome P450 family 2 subfamily C member 8
            (CYP2C8), transcript variant 1, mRNA.
ACCESSION   NM_000770
VERSION     NM_000770.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Almestica-Roberts,M., Nguyen,N.D., Sun,L., Serna,S.N., Rapp,E.,
            Burrell-Gerbers,K.L., Memon,T.A., Stone,B.L., Nkoy,F.L., Lamb,J.G.,
            Deering-Rice,C.E., Rower,J.E. and Reilly,C.A.
  TITLE     The Cytochrome P450 2C8*3 Variant (rs11572080) Is Associated with
            Improved Asthma Symptom Control in Children and Altered Lipid
            Mediator Production and Inflammatory Response in Human Bronchial
            Epithelial Cells
  JOURNAL   Drug Metab Dispos 52 (8), 836-846 (2024)
   PUBMED   38772712
  REMARK    GeneRIF: The Cytochrome P450 2C8*3 Variant (rs11572080) Is
            Associated with Improved Asthma Symptom Control in Children and
            Altered Lipid Mediator Production and Inflammatory Response in
            Human Bronchial Epithelial Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Incze,E., Mango,K., Fekete,F., Kiss,A.F., Poti,A., Harko,T.,
            Moldvay,J., Szuts,D. and Monostory,K.
  TITLE     Potential Association of Cytochrome P450 Copy Number Alteration in
            Tumour with Chemotherapy Resistance in Lung Adenocarcinoma Patients
  JOURNAL   Int J Mol Sci 24 (17), 13380 (2023)
   PUBMED   37686184
  REMARK    GeneRIF: Potential Association of Cytochrome P450 Copy Number
            Alteration in Tumour with Chemotherapy Resistance in Lung
            Adenocarcinoma Patients.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1924)
  AUTHORS   Lee,Y.B., Kim,V., Lee,S.G., Lee,G.H., Kim,C., Jeong,E. and Kim,D.
  TITLE     Functional Characterization of Allelic Variations of Human
            Cytochrome P450 2C8 (V181I, I244V, I331T, and L361F)
  JOURNAL   Int J Mol Sci 24 (9), 8032 (2023)
   PUBMED   37175734
  REMARK    GeneRIF: Functional Characterization of Allelic Variations of Human
            Cytochrome P450 2C8 (V181I, I244V, I331T, and L361F).
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1924)
  AUTHORS   Li,Y., You,C., Liu,Z., He,F., Zhao,F., Song,X., Xie,Z., Wei,S.,
            Yang,Y., Wei,H., Che,F. and Yu,J.
  TITLE     CYP2C8 and CYP2J2 gene variations increase the risk of hypertensive
            intracerebral hemorrhage
  JOURNAL   J Stroke Cerebrovasc Dis 32 (3), 106974 (2023)
   PUBMED   36587509
  REMARK    GeneRIF: CYP2C8 and CYP2J2 gene variations increase the risk of
            hypertensive intracerebral hemorrhage.
REFERENCE   5  (bases 1 to 1924)
  AUTHORS   Muradian,A.A., Sychev,D.A., Blagovestnov,D.A., Petrov,D.I.,
            Skukin,D.S., Epifanova,I.P., Sozaeva,Z.A., Kachanova,A.A.,
            Denisenko,N.P., Abdullaev,S.P. and Grishina,E.A.
  TITLE     [Evaluation of the association of polymorphisms of the CYP2C8 gene
            with the efficacy and safety of ketorolac in patients with
            postoperative pain syndrome]
  JOURNAL   Ter Arkh 94 (5), 610-615 (2022)
   PUBMED   36286958
  REMARK    GeneRIF: [Evaluation of the association of polymorphisms of the
            CYP2C8 gene with the efficacy and safety of ketorolac in patients
            with postoperative pain syndrome].
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1924)
  AUTHORS   Nelson,D.R., Zeldin,D.C., Hoffman,S.M., Maltais,L.J., Wain,H.M. and
            Nebert,D.W.
  TITLE     Comparison of cytochrome P450 (CYP) genes from the mouse and human
            genomes, including nomenclature recommendations for genes,
            pseudogenes and alternative-splice variants
  JOURNAL   Pharmacogenetics 14 (1), 1-18 (2004)
   PUBMED   15128046
  REMARK    Review article
REFERENCE   7  (bases 1 to 1924)
  AUTHORS   Romkes,M., Faletto,M.B., Blaisdell,J.A., Raucy,J.L. and
            Goldstein,J.A.
  TITLE     Cloning and expression of complementary DNAs for multiple members
            of the human cytochrome P450IIC subfamily
  JOURNAL   Biochemistry 30 (13), 3247-3255 (1991)
   PUBMED   2009263
  REMARK    Erratum:[Biochemistry. 1993 Feb 9;32(5):1390. doi:
            10.1021/bi00056a025.. PMID: 8095407]
REFERENCE   8  (bases 1 to 1924)
  AUTHORS   Ged,C. and Beaune,P.
  TITLE     Isolation of the human cytochrome P-450 IIC8 gene: multiple
            glucocorticoid responsive elements in the 5' region
  JOURNAL   Biochim Biophys Acta 1088 (3), 433-435 (1991)
   PUBMED   1707679
REFERENCE   9  (bases 1 to 1924)
  AUTHORS   Kolyada,A.Y.
  TITLE     Sequence of a human liver cytochrome P-450 cDNA clone
  JOURNAL   Nucleic Acids Res 18 (18), 5550 (1990)
   PUBMED   2216732
REFERENCE   10 (bases 1 to 1924)
  AUTHORS   Shephard,E.A., Phillips,I.R., Santisteban,I., Palmer,C.N. and
            Povey,S.
  TITLE     Cloning, expression and chromosomal localization of a member of the
            human cytochrome P450IIC gene sub-family
  JOURNAL   Ann Hum Genet 53 (1), 23-31 (1989)
   PUBMED   2729895
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC020596.1, Y00498.1 and
            M21941.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 19, 2006 this sequence version replaced NM_000770.2.
            
            Summary: This gene encodes a member of the cytochrome P450
            superfamily of enzymes. The cytochrome P450 proteins are
            monooxygenases which catalyze many reactions involved in drug
            metabolism and synthesis of cholesterol, steroids and other lipids.
            This protein localizes to the endoplasmic reticulum and its
            expression is induced by phenobarbital. The enzyme is known to
            metabolize many xenobiotics, including the anticonvulsive drug
            mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer
            drug taxol. This gene is located within a cluster of cytochrome
            P450 genes on chromosome 10q24. Several transcript variants
            encoding a few different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC020596.1, Y00498.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162895, SAMN04284274
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371270.6/ ENSP00000360317.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-338               BC020596.1         1-338
            339-1591            Y00498.1           286-1538
            1592-1924           M21941.1           1467-1799
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q23.33"
     gene            1..1924
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /note="cytochrome P450 family 2 subfamily C member 8"
                     /db_xref="GeneID:1558"
                     /db_xref="HGNC:HGNC:2622"
                     /db_xref="MIM:601129"
     exon            1..263
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     CDS             96..1568
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /EC_number="1.14.14.1"
                     /note="isoform a is encoded by transcript variant 1;
                     microsomal monooxygenase; xenobiotic monooxygenase;
                     flavoprotein-linked monooxygenase; P450 form 1; cytochrome
                     P450, family 2, subfamily C, polypeptide 8; cytochrome
                     P450, subfamily IIC (mephenytoin 4-hydroxylase),
                     polypeptide 8; cytochrome P450 2C8; s-mephenytoin
                     4-hydroxylase; cytochrome P450 IIC2; cytochrome P450
                     MP-12; cytochrome P450 MP-20; cytochrome P450 form 1"
                     /codon_start=1
                     /product="cytochrome P450 2C8 isoform a"
                     /protein_id="NP_000761.3"
                     /db_xref="CCDS:CCDS7438.1"
                     /db_xref="GeneID:1558"
                     /db_xref="HGNC:HGNC:2622"
                     /db_xref="MIM:601129"
                     /translation="MEPFVVLVLCLSFMLLFSLWRQSCRRRKLPPGPTPLPIIGNMLQ
                     IDVKDICKSFTNFSKVYGPVFTVYFGMNPIVVFHGYEAVKEALIDNGEEFSGRGNSPI
                     SQRITKGLGIISSNGKRWKEIRRFSLTTLRNFGMGKRSIEDRVQEEAHCLVEELRKTK
                     ASPCDPTFILGCAPCNVICSVVFQKRFDYKDQNFLTLMKRFNENFRILNSPWIQVCNN
                     FPLLIDCFPGTHNKVLKNVALTRSYIREKVKEHQASLDVNNPRDFIDCFLIKMEQEKD
                     NQKSEFNIENLVGTVADLFVAGTETTSTTLRYGLLLLLKHPEVTAKVQEEIDHVIGRH
                     RSPCMQDRSHMPYTDAVVHEIQRYSDLVPTGVPHAVTTDTKFRNYLIPKGTTIMALLT
                     SVLHDDKEFPNPNIFDPGHFLDKNGNFKKSDYFMPFSAGKRICAGEGLARMELFLFLT
                     TILQNFNLKSVDDLKNLNTTAVTKGIVSLPPSYQICFIPV"
     exon            264..426
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     exon            427..576
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     exon            577..737
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     exon            738..914
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1056
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1244
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1386
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1924
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1896..1901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /note="hexamer: AATAAA"
     polyA_site      1924
                     /gene="CYP2C8"
                     /gene_synonym="CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20"
                     /note="major polyA site"
ORIGIN      
        1 acatgtcaaa gagacacaca ctaaattagc agggagtgtt ataaaaactt tggagtgcaa
       61 gctcacagct gtcttaataa gaagagaagg cttcaatgga accttttgtg gtcctggtgc
      121 tgtgtctctc ttttatgctt ctcttttcac tctggagaca gagctgtagg agaaggaagc
      181 tccctcctgg ccccactcct cttcctatta ttggaaatat gctacagata gatgttaagg
      241 acatctgcaa atctttcacc aatttctcaa aagtctatgg tcctgtgttc accgtgtatt
      301 ttggcatgaa tcccatagtg gtgtttcatg gatatgaggc agtgaaggaa gccctgattg
      361 ataatggaga ggagttttct ggaagaggca attccccaat atctcaaaga attactaaag
      421 gacttggaat catttccagc aatggaaaga gatggaagga gatccggcgt ttctccctca
      481 caaccttgcg gaattttggg atggggaaga ggagcattga ggaccgtgtt caagaggaag
      541 ctcactgcct tgtggaggag ttgagaaaaa ccaaggcttc accctgtgat cccactttca
      601 tcctgggctg tgctccctgc aatgtgatct gctccgttgt tttccagaaa cgatttgatt
      661 ataaagatca gaattttctc accctgatga aaagattcaa tgaaaacttc aggattctga
      721 actccccatg gatccaggtc tgcaataatt tccctctact cattgattgt ttcccaggaa
      781 ctcacaacaa agtgcttaaa aatgttgctc ttacacgaag ttacattagg gagaaagtaa
      841 aagaacacca agcatcactg gatgttaaca atcctcggga ctttatcgat tgcttcctga
      901 tcaaaatgga gcaggaaaag gacaaccaaa agtcagaatt caatattgaa aacttggttg
      961 gcactgtagc tgatctattt gttgctggaa cagagacaac aagcaccact ctgagatatg
     1021 gactcctgct cctgctgaag cacccagagg tcacagctaa agtccaggaa gagattgatc
     1081 atgtaattgg cagacacagg agcccctgca tgcaggatag gagccacatg ccttacactg
     1141 atgctgtagt gcacgagatc cagagataca gtgaccttgt ccccaccggt gtgccccatg
     1201 cagtgaccac tgatactaag ttcagaaact acctcatccc caagggcaca accataatgg
     1261 cattactgac ttccgtgcta catgatgaca aagaatttcc taatccaaat atctttgacc
     1321 ctggccactt tctagataag aatggcaact ttaagaaaag tgactacttc atgcctttct
     1381 cagcaggaaa acgaatttgt gcaggagaag gacttgcccg catggagcta tttttatttc
     1441 taaccacaat tttacagaac tttaacctga aatctgttga tgatttaaag aacctcaata
     1501 ctactgcagt taccaaaggg attgtttctc tgccaccctc ataccagatc tgcttcatcc
     1561 ctgtctgaag aatgctagcc catctggctg ccgatctgct atcacctgca actctttttt
     1621 tatcaaggac attcccacta ttatgtcttc tctgacctct catcaaatct tcccattcac
     1681 tcaatatccc ataagcatcc aaactccatt aaggagagtt gttcaggtca ctgcacaaat
     1741 atatctgcaa ttattcatac tctgtaacac ttgtattaat tgctgcatat gctaatactt
     1801 ttctaatgct gactttttaa tatgttatca ctgtaaaaca cagaaaagtg attaatgaat
     1861 gataatttag atccatttct tttgtgaatg tgctaaataa aaagtgttat taattgctgg
     1921 ttca
//
