LOCUS       NM_177538              10552 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens cytochrome P450 family 20 subfamily A member 1
            (CYP20A1), transcript variant 2, mRNA.
ACCESSION   NM_177538
VERSION     NM_177538.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Liang,T., Liang,A., Zhang,X., Wang,Q., Wu,H., He,J. and Jin,T.
  TITLE     The association study between CYP20A1, CYP4F2, CYP2D6 gene
            polymorphisms and coronary heart disease risk in the Han population
            in southern China
  JOURNAL   Genes Genomics 44 (9), 1125-1135 (2022)
   PUBMED   34302632
  REMARK    GeneRIF: The association study between CYP20A1, CYP4F2, CYP2D6 gene
            polymorphisms and coronary heart disease risk in the Han population
            in southern China.
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 10552)
  AUTHORS   Bhattacharya,A., Jha,V., Singhal,K., Fatima,M., Singh,D.,
            Chaturvedi,G., Dholakia,D., Kutum,R., Pandey,R., Bakken,T.E.,
            Seth,P., Pillai,B. and Mukerji,M.
  TITLE     Multiple Alu Exonization in 3'UTR of a Primate-Specific Isoform of
            CYP20A1 Creates a Potential miRNA Sponge
  JOURNAL   Genome Biol Evol 13 (1) (2021)
   PUBMED   33434274
  REMARK    GeneRIF: Multiple Alu Exonization in 3'UTR of a Primate-Specific
            Isoform of CYP20A1 Creates a Potential miRNA Sponge.
REFERENCE   4  (bases 1 to 10552)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 10552)
  AUTHORS   Croteau-Chonka,D.C., Marvelle,A.F., Lange,E.M., Lee,N.R.,
            Adair,L.S., Lange,L.A. and Mohlke,K.L.
  TITLE     Genome-wide association study of anthropometric traits and evidence
            of interactions with age and study year in Filipino women
  JOURNAL   Obesity (Silver Spring) 19 (5), 1019-1027 (2011)
   PUBMED   20966902
REFERENCE   6  (bases 1 to 10552)
  AUTHORS   Ross,C.J., Katzov-Eckert,H., Dube,M.P., Brooks,B., Rassekh,S.R.,
            Barhdadi,A., Feroz-Zada,Y., Visscher,H., Brown,A.M., Rieder,M.J.,
            Rogers,P.C., Phillips,M.S., Carleton,B.C. and Hayden,M.R.
  CONSRTM   CPNDS Consortium
  TITLE     Genetic variants in TPMT and COMT are associated with hearing loss
            in children receiving cisplatin chemotherapy
  JOURNAL   Nat Genet 41 (12), 1345-1349 (2009)
   PUBMED   19898482
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Nat Genet. 2013 May;45(5):578]
REFERENCE   7  (bases 1 to 10552)
  AUTHORS   Jiang,J.H., Jia,W.H., Qin,H.D., Liang,H., Pan,Z.G. and Zeng,Y.X.
  TITLE     [Expression of cytochrome P450 enzymes in human nasopharyngeal
            carcinoma and non-cancerous nasopharynx tissue]
  JOURNAL   Ai Zheng 23 (6), 672-677 (2004)
   PUBMED   15191668
REFERENCE   8  (bases 1 to 10552)
  AUTHORS   Nelson,D.R., Zeldin,D.C., Hoffman,S.M., Maltais,L.J., Wain,H.M. and
            Nebert,D.W.
  TITLE     Comparison of cytochrome P450 (CYP) genes from the mouse and human
            genomes, including nomenclature recommendations for genes,
            pseudogenes and alternative-splice variants
  JOURNAL   Pharmacogenetics 14 (1), 1-18 (2004)
   PUBMED   15128046
  REMARK    Review article
REFERENCE   9  (bases 1 to 10552)
  AUTHORS   Werck-Reichhart,D. and Feyereisen,R.
  TITLE     Cytochromes P450: a success story
  JOURNAL   Genome Biol 1 (6), REVIEWS3003 (2000)
   PUBMED   11178272
  REMARK    Review article
REFERENCE   10 (bases 1 to 10552)
  AUTHORS   Smith,G., Stubbins,M.J., Harries,L.W. and Wolf,C.R.
  TITLE     Molecular genetics of the human cytochrome P450 monooxygenase
            superfamily
  JOURNAL   Xenobiotica 28 (12), 1129-1165 (1998)
   PUBMED   9890157
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011737.10 and AC080075.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 23, 2019 this sequence version replaced NM_177538.2.
            
            Summary: This gene encodes a member of the cytochrome P450
            superfamily of enzymes. The cytochrome P450 proteins are
            monooxygenases that catalyze many reactions involved in drug
            metabolism and synthesis of cholesterol, steroids and other lipids.
            This protein lacks one amino acid of the conserved heme binding
            site. It also lacks the conserved I-helix motif AGX(D,E)T,
            suggesting that its substrate may carry its own oxygen. [provided
            by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.3693718.1,
                                           SRR1660809.169218.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356079.9/ ENSP00000348380.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-117               AC011737.10        121110-121226
            118-167             AC011737.10        127938-127987
            168-334             AC011737.10        128847-129013
            335-477             AC011737.10        134059-134201
            478-645             AC011737.10        148606-148773
            646-724             AC011737.10        154762-154840
            725-840             AC011737.10        160665-160780
            841-895             AC011737.10        162151-162205
            896-1016            AC011737.10        167704-167824
            1017-1128           AC011737.10        171857-171968
            1129-1193           AC011737.10        174354-174418
            1194-1283           AC080075.7         3857-3946
            1284-10552          AC080075.7         4141-13409
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.2"
     gene            1..10552
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="cytochrome P450 family 20 subfamily A member 1"
                     /db_xref="GeneID:57404"
                     /db_xref="HGNC:HGNC:20576"
     exon            1..117
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..1434
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="isoform 2 is encoded by transcript variant 2;
                     cytochrome P450 monooxygenase; cytochrome P450 20A1;
                     cytochrome P450, family 20, subfamily A, polypeptide 1"
                     /codon_start=1
                     /product="cytochrome P450 20A1 isoform 2"
                     /protein_id="NP_803882.1"
                     /db_xref="CCDS:CCDS2357.1"
                     /db_xref="GeneID:57404"
                     /db_xref="HGNC:HGNC:20576"
                     /translation="MLDFAIFAVTFLLALVGAVLYLYPASRQAAGIPGITPTEEKDGN
                     LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTSDPFET
                     MLKSLLRYQSGGGSVSENHMRKKLYENGVTDSLKSNFALLLKLSEELLDKWLSYPETQ
                     HVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGTVWSEIGKGFLDGSLDKNM
                     TRKKQYEDALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQILEDSMIFSLA
                     SCIITAKLCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLRYCQHVLCETV
                     RTAKLTPVSAQLQDIEGKIDRFIIPRETLVLYALGVVLQDPNTWPSPHKFDPDRFDDE
                     LVMKTFSSLGFSGTQECPELRFAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSS
                     REEAWITVSKRY"
     exon            118..167
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            168..334
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            335..477
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            478..645
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            646..724
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            725..840
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            841..895
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1016
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1128
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1193
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1283
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..10552
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1535..1540
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="hexamer: TATAAA"
     polyA_site      1557
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="major polyA site"
     regulatory      10118..10123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="hexamer: AATAAA"
     polyA_site      10137
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
     regulatory      10286..10291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="hexamer: AGTAAA"
     polyA_site      10309
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
     regulatory      10344..10349
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="hexamer: AATAAA"
     polyA_site      10366
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
     regulatory      10513..10518
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="hexamer: AATATA"
     regulatory      10536..10541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
                     /note="hexamer: ACTAAA"
     polyA_site      10542
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
     polyA_site      10552
                     /gene="CYP20A1"
                     /gene_synonym="CYP-M"
ORIGIN      
        1 gctggagcgg ccgatccgag acgtggctcc ctgggcggca gaaccatgtt ggacttcgcg
       61 atcttcgccg ttaccttctt gctggcgttg gtgggagccg tgctctacct ctatccggct
      121 tccagacaag ctgcaggaat tccagggatt actccaactg aagaaaaaga tggtaatctt
      181 ccagatattg tgaatagtgg aagtttgcat gagttcctgg ttaatttgca tgagagatat
      241 gggcctgtgg tctccttctg gtttggcagg cgcctcgtgg ttagtttggg cactgttgat
      301 gtactgaagc agcatatcaa tcccaataag acatcggacc cttttgaaac catgctgaag
      361 tcattattaa ggtatcaatc tggtggtggc agtgtgagtg aaaaccacat gaggaaaaaa
      421 ttgtatgaaa atggtgtgac tgattctctg aagagtaact ttgccctcct cctaaagctt
      481 tcagaagaat tattagataa atggctctcc tacccagaga cccagcacgt gcccctcagc
      541 cagcatatgc ttggttttgc tatgaagtct gttacacaga tggtaatggg tagtacattt
      601 gaagatgatc aggaagtcat tcgcttccag aagaatcatg gcacagtttg gtctgagatt
      661 ggaaaaggct ttctagatgg gtcacttgat aaaaacatga ctcggaaaaa acaatatgaa
      721 gatgccctca tgcaactgga gtctgtttta aggaacatca taaaagaacg aaaaggaagg
      781 aacttcagtc aacatatttt cattgactcc ttagtacaag ggaaccttaa tgaccaacag
      841 atcctagaag acagtatgat attttctctg gccagttgca taataactgc aaaattgtgt
      901 acctgggcaa tctgtttttt aaccacctct gaagaagttc aaaaaaaatt atatgaagag
      961 ataaaccaag tttttggaaa tggtcctgtt actccagaga aaattgagca gctcagatat
     1021 tgtcagcatg tgctttgtga aactgttcga actgccaaac tgactccagt ttctgcccag
     1081 cttcaagata ttgaaggaaa aattgaccga tttattattc ctagagagac cctcgtcctt
     1141 tatgcccttg gtgtggtact tcaggatcct aatacttggc catctccaca caagtttgat
     1201 ccagatcggt ttgatgatga attagtaatg aaaacttttt cctcacttgg attctcaggc
     1261 acacaggagt gtccagagtt gaggtttgca tatatggtga ccacagtact tcttagtgta
     1321 ttggtgaaga gactgcacct actttctgtg gagggacagg ttattgaaac aaagtatgaa
     1381 ctggtaacat catcaaggga agaagcttgg atcactgtct caaagagata ttaaaatttt
     1441 atacatttaa aatcattgtt aaattgattg aggaaaacaa ccatttaaaa aaaatctatg
     1501 ttgaatcctt ttataaacca gtatcacttt gtaatataaa cacctatttg tacttaattt
     1561 tgtaaatttg gatttttata tatcatattt tcttaattca ttgtacacat ttgacttact
     1621 gcacagtata ttgatcattt taatgggaaa ctttagcttt ctacttttta tttttgtttt
     1681 ttcactttct atgccattat ttttgtattc tttttcttag tgtgagctct aaaatcaatg
     1741 ttcttgaaaa agaaattatt ttgcagaagt tggggaatca tgtttgttga atatgtataa
     1801 aatagaaaca taggctgggc gcggtggctc acacctgtaa tccctacact ttgggaggct
     1861 gaggcaggtg gatcacctga ggtccagagt ttgagaccag tctggccaac atgatgaaac
     1921 cccatctcta ctaaaaatac aaaacattgg ccgggagtgg tggctcatgc ctgtaatccc
     1981 agcactttgg gatgctgagg cgggtggatc acctgaggtc aggagtttgc gaccagcctg
     2041 gccaacatga tgaaaccctg tctctactaa aaatacaaaa aaattggctg ggtgtggtgg
     2101 cccacacctg taatcccagc actttgggag gtcgaggcgg gtggatcacc tgaggtccga
     2161 agttcgaggc cagcctggcc aacaggatga aaccctgtct ctattaaaaa tacagaaaat
     2221 tggccgggtg cggtggctca cccctgtaat cccagtactt tgggaggctg aggcgggtgg
     2281 agcacctgag gtcaggaatt cgagatcagc ctggccaaca tggtgaaacc ccatctctac
     2341 tgaaaaacac acacaaaaaa attagctggg catggtggca catgcctgta atcccagcta
     2401 ctcaggaggc tgaggcagga gaatcatttg aacctgggag gcggagcttg cagtgagccg
     2461 agattgcacc cctgcactcc agcctgggcc acagagcaag actctgtctc aagaaaaaca
     2521 aaaaaaaaga taaatggaca aaagacatga acaaacagct tttataggat agtatggcta
     2581 acaaactttt agaaaagtgt ttagtctcac tagtaagtaa caaaatattg gggcagggca
     2641 cagtggctca tgcgtataat cctagaactt tggaaggcca aagctggtgg attgcttgag
     2701 cccaggagtt tgagaccagc ccaggcaaca ttgggagacc ctgcctctac aaatatacaa
     2761 aaaaattact gggcatggtg acacacacct gtagtcccag ctgttctgaa ggctgaggtg
     2821 ggaggatcac ttgagaccag gagaggtcaa ggctgcagtg atctgtgatc acaccactgc
     2881 actccagcct gggcaacaga gcaaaaccct gcctttaaaa aaacaaaaaa caaaaaaggc
     2941 cgggctcggt ggctcatgcc tataatactt tgggaggccg aggcaggtgg atcaattgag
     3001 gtcaggtgtt cgagaccagc caggccaact tggtgaaacc ccgtctctac taaaaataca
     3061 aaaaaaaaaa aaaaaaatta gccagatgtg gtggtgcaca tttgtaatcc cagctactca
     3121 ggaggctgag gcaggataat cgcttgaacc tgggaggcag aggttgcagt gagccaagat
     3181 cgcaccactg cactccagcc tgggtgtcga gtgaaactca ttctcaaaaa aaaaaaaaaa
     3241 aggaggtggg gagagctatt tcaagaatat tttaagtagg ctgggcacgg tagctcatgc
     3301 ctgttatcct aacactttgg gaagccgaca tgggcagatc acgaggtcaa gagatttgag
     3361 accatcctgg ccaacatggt gaaaccccat ctctactaaa aatacaaaaa attatctgga
     3421 catgctggcg tgcgcctgta gtctcagcta cttgggaggc tgaggcagga gaatcgcttg
     3481 aacctgggag gcggaggttg cagtgagctg atgttgtgct agtgcactcc agcctgggcg
     3541 acagggctga gaccttgtct caaaaaaaaa aggagtatct taagtgtctt tcaaaattac
     3601 actttagcac ttataaattt tatatataat actttactga tagcattgta tattagtcca
     3661 acttttttgg ctacactatt tgtgaaggat tccttttttt ctttaagaca gccactgtag
     3721 gaaatatgcc aagtacagat agtgaggaaa gaaaggaaga aaaatgataa ttgtctgcaa
     3781 gtgggcttat ttttaacact gctgaatttc atttgtaatt ttactttcaa gaatatactc
     3841 tggtagactg aggcgggagg atcacttgag cccaggagtt ggaggcttca gtgagctgtg
     3901 atcacaccac tgccctcagc caaacctgag tgataaagtg agaccctatc tcttaaaaaa
     3961 aaaaatccct ttgttgttga gttattttat gtcatttatt ggtaaatacc taacatttta
     4021 gacctagtca ttaccagtta acatagaatt taattggatg cagtctgtat ttttgaaaaa
     4081 aaaaattttt tttggaataa cttaaattct aaaactttgg ccgggcgtga tggctcatgc
     4141 ctgtaatccc agcactttgg gaggccgagg caggcagatc acctgaggtc aggcgttcaa
     4201 gaccagcctg accaacatgg agaaaccccg tctctactaa aaatacaaaa ttagccaggc
     4261 ttggtggtgc atgcctgtaa taccagctac tcgggaggct gaagcaggag aactgcttga
     4321 acctgggagg cagaggttgc ggtgagccaa gatcgcgcca ttgcactcca gcctgggcaa
     4381 caagagtgaa actccgtctc aaaaaaaaaa gaaaaattct aaaactttaa gtgaaattta
     4441 ttatcccttc ccacttattt tctgtggttt tgtgtgtata gagcatcttc ctttgttacc
     4501 caagtacctc gaatgggttg gaaactggcg gtagaaggtt agagtagcca attgatatgt
     4561 aaaccaagtg tcagatcaga tgagatgaaa gaggtggagt ttaaagcaag tgagatatga
     4621 taaacaatga tgaaagcaaa aatagggcag ttgaaccaga gtcagccagt tggcataact
     4681 gccaataaat gaggcagtgt agaataacta ataatgattg gaatcaagga aaagagagca
     4741 tagaggaatc agctaaatta gtatgtttta tccatagtta catgactaac aaatgattaa
     4801 aaagaagtta ttctctagat aattttttaa ataaaagtac tgttaatata taaactaagt
     4861 tattcattca ctgtttttct tcacagtaag aatgacaaaa atctgtgttc tttgtactta
     4921 tcaagtgact caaattttgg ccacttcact gtcactgatg gcttatagaa tgattctagc
     4981 aggactcaag ttattgaact atcaaatggg tttttaattg atattgaagt aagtaagttt
     5041 tgtagctttt ggagttttta aaaactactt cagtgatttt gatttatatt ctttttgata
     5101 gttcagaagt gtattgttaa aaaacataag catggccggg cacagtggct cacacctgta
     5161 atcccagctc tttgggaggc caaggcgggt aaatcacctg aggttgggag tttgagacca
     5221 gcctgaccaa catggagaaa ccctgtctct accaaaaata caaaattagc cgggcatgat
     5281 ggagcatccc tgtaatccta gctacttgag acgctgaggc aggaaaattg cttgaacccg
     5341 tgaggcagag gttgcgatga gccgagatcg tgccattgca ctccagcctg ggcaataaga
     5401 gcaaaactcc gtctcagaaa aaaaaaaaaa aaaaaaaaac ggccaggcga ggtggctcac
     5461 acctgtaatc ccagcacttt gggaggctga ggcgggcaga tcacctgaag tcaggagttc
     5521 aagaccagcc tgaccaacat ggagaaactt catctctact aaaaatacaa aattagccgg
     5581 gcgtggtggc acatgccttt aatcccagct actggggagg ctgaaacagg agaatcgctt
     5641 gaacccagta ggggaaggtt gtggtgagcc aagattgcac cattgcactc cagcctgggc
     5701 aagaagagtg aaactctatc caaaaaaaaa aaaaaaccat acgtacataa ctttttttct
     5761 ttttcttttc tttctttctt ttcttttctt tttttttttt gaggcacagt cttgctttgt
     5821 tgaccaagct ggagtgcatt ggcatgatca cagcttactg tagcctccac ctcctgggct
     5881 ccagcgatct cacctcagcc tctagaatag ctgggactac atatgcatgc caccatgccc
     5941 agctgttttt ttgtttgctt gtttttggtt ttgttttttt gagacagggt ctccctgcgt
     6001 cgcccagtct tgtctcaaac tcctaggctc aagcgatcct cccacctggg cctcccaaag
     6061 tgttgagatt gcaggtgtga gccacggcac ctggtccata aaacattttt ttatgaacta
     6121 aagagctctc tcaaacttgt gctaacaaaa tttaaggcaa tattaattct gtgcatgtgt
     6181 ttgggaaaat taaaaaaact tacatagtgc ttatttggcc cacaaaagta cagttccatt
     6241 ttttatataa atagattcta cctaacttat aacccctatg tcaaaatttg tttatcttaa
     6301 aatagtttgt ataaaaagtc tttactgctt tattatgaaa taataatgtt tattgtagaa
     6361 aaatctagga aaacacaaaa atgtcaaact cagttaacag tcatagtttc actatttgaa
     6421 gttaaccact gttaagattc tttttttttt tttttttttt ttgttttgag actgagtccc
     6481 gctgtctatt gcccaggctg gagtgccatg gcaccatctc ggctcactgc aacctctgct
     6541 tcccaggttc aagtgatcct cccgcctcag cctcccaagt agctgggatt gcaaacatgt
     6601 gccaccacac ctggctaatt tttgtgtttt tagtacagat ggggtttcac catgttggcc
     6661 aggctggcct cgaactcctc aacctcaagt agtctgccca ccttgacctc ccaaagtgct
     6721 gggattacag gcatggggca ccatgcccag ccccactgtt aagattctaa tgtaggccgg
     6781 gcacagtgac tcatgcctat aactcaagca ctttgggagt tcaaggcggc cagattgctt
     6841 gagcttttag aagtttgaga ccagcctggg caacgtggca aaagcttgtt tctataaaaa
     6901 aatataaaaa cttagccagc cttggtggca ccacctgcag tcccaactat tcaggaggct
     6961 gaggcgagag gatggcttga gctcaggaag tcaaggctac agtgagctgt ggtagtgcca
     7021 ctgcactccc tcatggttga cagagcaaga ccctgtctca aaagattcta atatagatac
     7081 tctttttatg catatgtaat ttatatatat tatacaaaat attatttgca tttaacatat
     7141 tctgaaccaa tagtcttttc tacaagcaga acattagtat tcttgtcact ctgaatgtag
     7201 gcacagattt ttgtcattct ttatcttttt tgtgtgtgtg tgacagagtc tcactgtcac
     7261 caggctggaa tgcagtggcg tgatctcggc tcactgcaac ctctgcctcc caggttcaag
     7321 cgattctctt gcctcagcct tttgagtaac tggggttaca ggcgcgtgcc atcacaccca
     7381 gctcattttt gtatttttag tagagatggg gttttaccgt gttggtcagg ctggtcctga
     7441 actcttgacc ttgtgatctg cccaactcag cctcccagag tgctgggatt acaagcatga
     7501 gccaccgcgc tcggtccctt tctttttatt tatttattta ttttgagatg gagtctcgct
     7561 gtgtcaccca ggctggagtg tggtggcacg atcttggctc actgcaaccc ctgcctccca
     7621 ggttcaagca attctcctgc ctcagcctcc tgagtagctg agactacggg tatgtgccac
     7681 tacactcagc taatttttgt attttttttt agtagagaca gggtttcacc atgttggcca
     7741 ggctggtctt gaactcctaa cctcaggtga ttcacacccc ttggcctcct gaagtgctgg
     7801 gattacaggc atgagccact gtgcccggcc atttttttgt gtttttagtg gagacaggtt
     7861 ttcaccatgt tggccagtag tgtatttagt tatttaagga gggctcaatc aacaggttta
     7921 aatggtgtgc tattcataat tattgcaaaa ataggccggg cctgtaattc cagcactttg
     7981 ggaggccaag gcaggtgaat cacttgaggc caggagtttg agatcagcct ggccaacgtg
     8041 gtgaaactcc atctctacta aaaataccaa aagtagcggg cgtggtgaca ggtgcctcta
     8101 atcccagcta ctcgggaggc tgaggcagga gaatcgcttg aacccaagag tcggaggttg
     8161 cagtgagccg agatcgcgcc attgcactcc agtgtgggtg acgagcgaaa atccgtctca
     8221 accaagaaaa aagaaaaaaa gaaaaagaaa acttaactgg cctttggggc acatgcctgt
     8281 aatcccagct tcttgggagg ctgaggcatg agaaaaaaaa taaacctggg aggtggaggt
     8341 tgcagtgagt tgagattgtg ccactgcact ccagcttagg caataaaaca agactgtctc
     8401 aaaaaaaaaa aaaaaaaaaa cttattgaga gaataatata ccactaatat ttaaagaaat
     8461 ttttggctgg tgccgtggct cacacctgta atcccagcac tttgggagtc caaagtgggc
     8521 gtataacttg aggtcaggag ttcaagaaca gcctggccaa catggggaaa ccgcatctct
     8581 actaaaaata caaaaattag tgggtgtgga ggcgggcgaa tgtaatccca gctagttgag
     8641 aggctgaggc aggagaatgt ctgggaggtg gaggttgcag tgtgccgaga tcgcaccact
     8701 gtactccagc ctaggtgaca gagtgagact ccataggtct cagccagact ctggaggtct
     8761 cagcccaggc ttgagtgcag tggcatgatc tgggctcact acaacctcca tctccccagt
     8821 tcaagcgatt ctcctgcctc agcctcccga gtagctgcga ctacaggcga gtgcctccat
     8881 gcccagctaa ttttttgtat ttttagtaga gacggggttt caccatgtta gccaggatgg
     8941 tcttgatctc ctgaccttgt gatctgtcca ccgcggcttc ccaaagtgct gggattacag
     9001 gtgtgagcca cagcgcccgg ccaaaaaaag gaatttttaa gaggaaaaag aatgctacca
     9061 acctaaacac atttctgtga ctgtttatat ttttccctgt tccacataca tacattttta
     9121 catagtacgt tcattgcagc atgagttact tttcacttaa taaattttaa acattttcca
     9181 gctgggtgtg gtggctcatg cctgtaatcc caacacttgg agaggccaag tcaggcttat
     9241 tgggtgagtc caggtgtttg agactagcct aggcaacatg gcgaaactgc atctctacaa
     9301 aaaatacaaa aattagccag gtgtgctggc gcacacctgt agtcccaact actcaggagg
     9361 ctgaggtggg aggattgctt gagcccagga ggtagaggtt gcagtgagcc atgatcatgc
     9421 cactgcactc cagcctaggt gacaaagcac aaccctcaaa taaataaata aatacatgtt
     9481 aaacatatac ctttattact tcatttttgt aagagcttca gggaataggc ttttttaaag
     9541 ggaatagact tatagatcaa ttttaatatt tgacttatag aattttatga cactaaatgg
     9601 ataattgtaa agtttgagtt gggacccatt ttttaagaca ttcaggctat aaacaaggta
     9661 ataaatcaca ctcttccagt tctactccca atttaattgg tttacagttc ggtggctgtc
     9721 tttttttttt tttttttttt tttttgagac cgagtctccc tctgtcacca ggctggagtg
     9781 cagtggtgca atctcagctc actgcaacct ccccctctca ggttcaagta attctcctgc
     9841 ctcagcctgc cgagtagctg ggactacagt cgcacgccaa ccacccccag ctaatttttt
     9901 tatttttagt agagacagga tttcaccatg tttgccagga tggtctcgat ctcttgtgct
     9961 cataatctac ctacctcggc ctcccaaagt gctcggatta cagacgtgag ccactgcgcc
    10021 cggcccgttg gcttgctttc tagatactgg gaatagatct tgttatagta tgtgataaat
    10081 agatcttgtt atagtatgtg ataaaatgcc atgatcaaat aaattactta tatcctgttt
    10141 ggtgattttt agaaaaacat atttatctac atctaaaata cttattttaa ttttaaacac
    10201 ttgatggtat gaaatgaaat tttgttactt taccattgta actatgtcat aaagtcataa
    10261 agtacctgtc cccaaaaagg caaccagtaa atatttttga gtaaatgaat gaaatcttta
    10321 ttgccggtgt tctgtgcaca tagaataaag gaaattatta atgttactta atagtttaaa
    10381 aaaattcaga agccaagcta tccataaagc tactttgtga tgtgctgaat actccattaa
    10441 tagaagtgtt taagaggatg ttgtatgcct gccttccact catgcctgtg ttagatgtga
    10501 acatagattt ataatatagt actatacaaa atacaactaa aatagttggt aa
//
