LOCUS       NM_024843               4235 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens cytochrome b reductase 1 (CYBRD1), transcript variant
            1, mRNA.
ACCESSION   NM_024843
VERSION     NM_024843.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Wang,X., Men,C., Shan,S., Yang,J., Zhang,S., Ji,X., Li,C. and
            Wang,Y.
  TITLE     EGFR upregulates miRNA subset to inhibit CYBRD1 and cause DDP
            resistance in gastric cancer
  JOURNAL   Gene 933, 149005 (2025)
   PUBMED   39419238
  REMARK    GeneRIF: EGFR upregulates miRNA subset to inhibit CYBRD1 and cause
            DDP resistance in gastric cancer.
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Zhao,G., Wang,Q., Zhang,Y., Gu,R., Liu,M., Li,Q., Zhang,J.,
            Yuan,H., Feng,T., Ou,D., Li,S., Li,S., Li,K., Mo,C. and Lin,P.
  TITLE     DDX17 induces epithelial-mesenchymal transition and metastasis
            through the miR-149-3p/CYBRD1 pathway in colorectal cancer
  JOURNAL   Cell Death Dis 14 (1), 1 (2023)
   PUBMED   36593242
  REMARK    GeneRIF: DDX17 induces epithelial-mesenchymal transition and
            metastasis through the miR-149-3p/CYBRD1 pathway in colorectal
            cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4235)
  AUTHORS   Ma,J., Huang,W., Zhu,C., Sun,X., Zhang,Q., Zhang,L., Qi,Q., Bai,X.,
            Feng,Y. and Wang,C.
  TITLE     miR-423-3p activates FAK signaling pathway to drive EMT process and
            tumor growth in lung adenocarcinoma through targeting CYBRD1
  JOURNAL   J Clin Lab Anal 35 (12), e24044 (2021)
   PUBMED   34714955
  REMARK    GeneRIF: miR-423-3p activates FAK signaling pathway to drive EMT
            process and tumor growth in lung adenocarcinoma through targeting
            CYBRD1.
REFERENCE   4  (bases 1 to 4235)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 4235)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 4235)
  AUTHORS   Su,D., May,J.M., Koury,M.J. and Asard,H.
  TITLE     Human erythrocyte membranes contain a cytochrome b561 that may be
            involved in extracellular ascorbate recycling
  JOURNAL   J Biol Chem 281 (52), 39852-39859 (2006)
   PUBMED   17068337
  REMARK    GeneRIF: Duodenal cytochrome b present in human erythrocytes may
            contribute to their ability to reduce extracellular
            monodehydroascorbate.
REFERENCE   7  (bases 1 to 4235)
  AUTHORS   Zaahl,M.G., Merryweather-Clarke,A.T., Kotze,M.J., van der Merwe,S.,
            Warnich,L. and Robson,K.J.
  TITLE     Analysis of genes implicated in iron regulation in individuals
            presenting with primary iron overload
  JOURNAL   Hum Genet 115 (5), 409-417 (2004)
   PUBMED   15338274
  REMARK    GeneRIF: In Africans with iron overload not related to the HFE
            gene, the possible involvement of the SLC40A1 and CYBRD1 genes was
            demonstrated for the first time.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 4235)
  AUTHORS   Vargas,J.D., Herpers,B., McKie,A.T., Gledhill,S., McDonnell,J., van
            den Heuvel,M., Davies,K.E. and Ponting,C.P.
  TITLE     Stromal cell-derived receptor 2 and cytochrome b561 are functional
            ferric reductases
  JOURNAL   Biochim Biophys Acta 1651 (1-2), 116-123 (2003)
   PUBMED   14499595
REFERENCE   9  (bases 1 to 4235)
  AUTHORS   Zoller,H., Theurl,I., Koch,R.O., McKie,A.T., Vogel,W. and Weiss,G.
  TITLE     Duodenal cytochrome b and hephaestin expression in patients with
            iron deficiency and hemochromatosis
  JOURNAL   Gastroenterology 125 (3), 746-754 (2003)
   PUBMED   12949720
REFERENCE   10 (bases 1 to 4235)
  AUTHORS   McKie,A.T., Barrow,D., Latunde-Dada,G.O., Rolfs,A., Sager,G.,
            Mudaly,E., Mudaly,M., Richardson,C., Barlow,D., Bomford,A.,
            Peters,T.J., Raja,K.B., Shirali,S., Hediger,M.A., Farzaneh,F. and
            Simpson,R.J.
  TITLE     An iron-regulated ferric reductase associated with the absorption
            of dietary iron
  JOURNAL   Science 291 (5509), 1755-1759 (2001)
   PUBMED   11230685
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL136693.1, CX866825.1, AK027115.1, CB266845.1, DB000953.1,
            AL834483.1 and AC007969.3.
            
            On Nov 23, 2018 this sequence version replaced NM_024843.3.
            
            Summary: This gene is a member of the cytochrome b(561) family that
            encodes an iron-regulated protein. It highly expressed in the
            duodenal brush border membrane. It has ferric reductase activity
            and is believed to play a physiological role in dietary iron
            absorption. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL136693.1, SRR1660809.135756.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321348.9/ ENSP00000319141.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AL136693.1         2-2
            2-844               CX866825.1         11-853
            845-868             AL136693.1         846-869
            869-1147            AK027115.1         829-1107
            1148-1332           AL136693.1         1149-1333
            1333-1850           CB266845.1         45-562
            1851-2105           AL136693.1         1852-2106
            2106-2642           DB000953.1         2-538
            2643-3019           AL136693.1         2644-3020
            3020-4057           AL834483.1         2300-3337
            4058-4231           AC007969.3         13690-13863         c
            4232-4235           AL136693.1         4233-4236
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..4235
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /note="cytochrome b reductase 1"
                     /db_xref="GeneID:79901"
                     /db_xref="HGNC:HGNC:20797"
                     /db_xref="MIM:605745"
     exon            1..265
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..933
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /EC_number="7.2.1.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     duodenal cytochrome b; ferric-chelate reductase 3;
                     cytochrome b561 family, member A2; plasma membrane
                     ascorbate-dependent reductase CYBRD1"
                     /codon_start=1
                     /product="plasma membrane ascorbate-dependent reductase
                     CYBRD1 isoform 1"
                     /protein_id="NP_079119.3"
                     /db_xref="CCDS:CCDS2244.1"
                     /db_xref="GeneID:79901"
                     /db_xref="HGNC:HGNC:20797"
                     /db_xref="MIM:605745"
                     /translation="MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSA
                     LEFNWHPVLMVTGFVFIQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVV
                     AVFENHNVNNIANMYSLHSWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHV
                     YSGIVIFGTVIATALMGLTEKLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWI
                     VTRPQWKRPKEPNSTILHPNGGTEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLA
                     LDEAGQRSTM"
     exon            266..474
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /inference="alignment:Splign:2.1.0"
     exon            475..629
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /inference="alignment:Splign:2.1.0"
     exon            630..4235
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4217..4222
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /note="hexamer: AATAAA"
     polyA_site      4235
                     /gene="CYBRD1"
                     /gene_synonym="CYB561A2; DCYTB; FRRS3"
                     /note="major polyA site"
ORIGIN      
        1 gccactaccc agagggctgc cgccgcctct ccaagttctt gtggcccccg cggtgcggag
       61 tatggggcgc tgatggccat ggagggctac tggcgcttcc tggcgctgct ggggtcggca
      121 ctgctcgtcg gcttcctgtc ggtgatcttc gccctcgtct gggtcctcca ctaccgagag
      181 gggcttggct gggatgggag cgcactagag tttaactggc acccagtgct catggtcacc
      241 ggcttcgtct tcatccaggg catcgccatc atcgtctaca gactgccgtg gacctggaaa
      301 tgcagcaagc tcctgatgaa atccatccat gcagggttaa atgcagttgc tgccattctt
      361 gcaattatct ctgtggtggc cgtgtttgag aaccacaatg ttaacaatat agccaatatg
      421 tacagtctgc acagctgggt tggactgata gctgtcatat gctatttgtt acagcttctt
      481 tcaggttttt cagtctttct gcttccatgg gctccgcttt ctctccgagc atttctcatg
      541 cccatacatg tttattctgg aattgtcatc tttggaacag tgattgcaac agcacttatg
      601 ggattgacag agaaactgat tttttccctg agagatcctg catacagtac attcccgcca
      661 gaaggtgttt tcgtaaatac gcttggcctt ctgatcctgg tgttcggggc cctcattttt
      721 tggatagtca ccagaccgca atggaaacgt cctaaggagc caaattctac cattcttcat
      781 ccaaatggag gcactgaaca gggagcaaga ggttccatgc cagcctactc tggcaacaac
      841 atggacaaat cagattcaga gttaaacagt gaagtagcag caaggaaaag aaacttagct
      901 ctggatgagg ctgggcagag atctaccatg taaaatgttg tagagataga gccatataac
      961 gtcacgtttc aaaactagct ctacagtttt gcttctccta ttagccatat gataattggg
     1021 ctatgtagta tcaatattta ctttaatcac aaaggatggt ttcttgaaat aatttgtatt
     1081 gattgaggcc tatgaactga cctgaattgg aaaggatgtg attaatataa ataatagcag
     1141 atataaattg tggttatgtt acctttatct tgttgaggac cacaacatta gcacggtgcc
     1201 ttgtgcagaa tagatactca atatgtgaat atgtgtctac tagtagttaa ttggataaac
     1261 tggcagcatc cctggcctgt tgtcatgcag tcatttcctg ttaattctgg gagacaatga
     1321 tttcacaact agcgggaagc agtcctaaaa gtttaaaatc cgataaggaa tatctgggac
     1381 agggtttaga tcatgactct acacagatac catgatgaga gtatattaaa gaaatttagg
     1441 aaagcacctg gttcctttct ccccatgcct gccttctgct ccctccccag ctggtttggg
     1501 ctcaaattgt ccctggagac tagggtttat gttagggtat tgatagatta gagcaggtgg
     1561 ttgaagagat cttctctggt cagacttgga agaatttcca aaactgaagt tagccccaag
     1621 acttccctag ggttgatgta ctttatgatc cagatgctaa acttcttaga atgaaaatat
     1681 gcttcaacac ttaagtagca tacactgccc tacaaacctc agagagcact tttccccaag
     1741 ttcttgtttt tatttttgaa agtactcaca cagcacttac tatgctccaa acactcctct
     1801 aagcacttta cacatattag ctcattcagt ccccagacag acgggatgaa gtaggtattg
     1861 ttactgttcc cattttacag gtgagagatt tgaagcctgg ggaggctagt aactcacccc
     1921 aaggtcacac ggctcataca tggtgggact gagactcaga tgcaggcagt ctggcacctc
     1981 agtctggatt ctaaccattt cactaagcta tttttgtctt gtactacttt gacccacccc
     2041 tgaataaacc tcaattgctg gagtggggtg tagttattaa agggatgctt tttacctttt
     2101 gctgtttgct gtggcagatt ccccagataa ccaaggaaaa ggggccaccc atacctggaa
     2161 ataggccata gggcccctac tactgccaac aagccatggc ctaccttgac acttgtttga
     2221 tcttaaaatt gtgtcttggt aacaaaagat ttggacaggc atatctgtag ctttcaagtt
     2281 aattaattgc aatatttttt tcttcaggat tttagctgct gaacaacttt cagtttggag
     2341 ctaaaagaga cctgtctcat ggtctgccct tccctggggc aatagctagg gtctttcctg
     2401 atttttatgg aattttaggg gatattttga gctttgggtt ctcagtagtg aattgagact
     2461 tggaggtgac ttttcatgtt tggagtatca tctctgtctg ggatctgggc tgacaaatta
     2521 aaacctagag tagtgcttat gctgaaatga tacttttcat tttttggttg atttttttgc
     2581 cttcccttca attttaaact gaagcatttt aatatgggta gaaactctac accaaataca
     2641 ctaaacattt tggtgcttag tggatttctt tttaggtaac tggtacttac ttccaaagac
     2701 tgaatacaag ccacactcca tcatatccct taaacttcat gaaaaaccat tcaagatccc
     2761 cttgctgcaa cactgttctc ttcttctcta ctaaattcta tttccaaaat tggtaataga
     2821 gccagaagga tccccagtac ccagccctct gcctggcaca aagtggtagc acaattaaat
     2881 tcagtatggg tggagcatgg tacagtcttg gtgccataga aggagtagtt gcatagtcac
     2941 acatcatttg ataagttgga tgttccatta catagaggaa cacaaaattc cagggttttt
     3001 ggaggaaggg attagatagt gactaagccg ccagaattga ggtggccatt cctttttgta
     3061 taggctaaga aacaggttat cagtgaaaag ttaattatgg ctttggcact agaatagcac
     3121 tgttgcaaag tatttaagca ccccccatct cagcccttta ttttatcttt catgtgggct
     3181 aatgtgagga taatcttaca gatattatag gaatttcttt tctatcttta tgaaaacaac
     3241 gtatataaaa tatatctaga aaacctttgt ttgagactct tatttaatgg gcttttgatt
     3301 ctaatgataa ttgtaccttt atctttcaaa agctgatatt tcctacctaa gcatctcccg
     3361 agaaaaatat ctcattaaaa agcccataaa taatagggga gaagaaagcc ttaggtatca
     3421 attccaaaac agtgattgaa atttcccaaa ataattatgg cttctgtcat ctccagagat
     3481 aatctggctt ggtttacccc ataatctaat ttcagaaaag aaagctttat tttaacactc
     3541 atctgaatca acattaaagc cttttctctc aaagcgttta ttgagaaact caaatgaata
     3601 tactttttga attactgtca tcaaaagtgt acggcttcct gtgctgcttg tgtcaaatgg
     3661 aacctgccct ctaaagcact ttctttcctt tacttgcgtg gtttcatgta agctgtgctg
     3721 tttagaaaca acatctcaga ctttacaaag aaatgacaaa gaaggcaatt gcacttttta
     3781 agggatatcg acaagcagtt tctgttttct aaaggacaaa atacagagtg tgtgtcattt
     3841 ttaattagat tctttcccct gctgagttgg aaattccagt gcagcactga ttgaccacag
     3901 ttgccaatct aaaagcacaa agacagaagt aaagctttat gctaatttta tttcaatatg
     3961 atagaaaatt tatcttggta tgtccttttt tagataactc cagcaggaaa ctgtaactgc
     4021 tatgtcttta ggaaaatgta gaagaaagaa cattattgtt ctttaattcc tacaaggtac
     4081 tcgaaaacct taagtgaaaa agatttctat ctttttatct tagcgcattt atggaaaaaa
     4141 tattaactat cctgaatatt ttataatttt gtaggaaaaa tatgcatcta ttttttcttg
     4201 acttctttta tatagtaata aaagttattt tggaa
//
