LOCUS       NM_016230               9205 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens cytochrome b5 reductase 4 (CYB5R4), mRNA.
ACCESSION   NM_016230
VERSION     NM_016230.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Benson,D.R., Deng,B., Kashipathy,M.M., Lovell,S., Battaile,K.P.,
            Cooper,A., Gao,P., Fenton,A.W. and Zhu,H.
  TITLE     The N-terminal intrinsically disordered region of Ncb5or docks with
            the cytochrome b5 core to form a helical motif that is of ancient
            origin
  JOURNAL   Proteins 92 (4), 554-566 (2024)
   PUBMED   38041394
  REMARK    GeneRIF: The N-terminal intrinsically disordered region of Ncb5or
            docks with the cytochrome b5 core to form a helical motif that is
            of ancient origin.
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Zambo,V., Toth,M., Schlachter,K., Szelenyi,P., Sarnyai,F., Lotz,G.,
            Csala,M. and Kereszturi,E.
  TITLE     Cytosolic localization of NADH cytochrome b oxidoreductase (Ncb5or)
  JOURNAL   FEBS Lett 590 (5), 661-671 (2016)
   PUBMED   26878259
  REMARK    GeneRIF: Cytosolic localization of NADH cytochrome b oxidoreductase
REFERENCE   3  (bases 1 to 9205)
  AUTHORS   Kalman,F.S., Lizak,B., Nagy,S.K., Meszaros,T., Zambo,V., Mandl,J.,
            Csala,M. and Kereszturi,E.
  TITLE     Natural mutations lead to enhanced proteasomal degradation of human
            Ncb5or, a novel flavoheme reductase
  JOURNAL   Biochimie 95 (7), 1403-1410 (2013)
   PUBMED   23523930
  REMARK    GeneRIF: data provide first evidence for natural mutations in
            NCB5OR gene resulting in decreased protein levels and hence having
            potential implications in human pathology.
REFERENCE   4  (bases 1 to 9205)
  AUTHORS   Percy,M.J., Barnes,C., Crighton,G., Leventer,R.J., Wynn,R. and
            Lappin,T.R.
  TITLE     Methemoglobin reductase deficiency: novel mutation is associated
            with a disease phenotype of intermediate severity
  JOURNAL   J Pediatr Hematol Oncol 34 (6), 457-460 (2012)
   PUBMED   22627575
  REMARK    GeneRIF: Mutation in cytochrome b5 reductase is associated with
            developmental delay and severe neurological problems.
REFERENCE   5  (bases 1 to 9205)
  AUTHORS   Bailey,S.D., Xie,C., Do,R., Montpetit,A., Diaz,R., Mohan,V.,
            Keavney,B., Yusuf,S., Gerstein,H.C., Engert,J.C. and Anand,S.
  CONSRTM   DREAM investigators
  TITLE     Variation at the NFATC2 locus increases the risk of
            thiazolidinedione-induced edema in the Diabetes REduction
            Assessment with ramipril and rosiglitazone Medication (DREAM) study
  JOURNAL   Diabetes Care 33 (10), 2250-2253 (2010)
   PUBMED   20628086
  REMARK    GeneRIF: Observational study of gene-disease association,
            gene-environment interaction, and pharmacogenomic / toxicogenomic.
            (HuGE Navigator)
REFERENCE   6  (bases 1 to 9205)
  AUTHORS   Deng,B., Parthasarathy,S., Wang,W., Gibney,B.R., Battaile,K.P.,
            Lovell,S., Benson,D.R. and Zhu,H.
  TITLE     Study of the individual cytochrome b5 and cytochrome b5 reductase
            domains of Ncb5or reveals a unique heme pocket and a possible role
            of the CS domain
  JOURNAL   J Biol Chem 285 (39), 30181-30191 (2010)
   PUBMED   20630863
  REMARK    GeneRIF: Study of the individual cytochrome b5 and cytochrome b5
            reductase domains of Ncb5or reveals a unique heme pocket and a
            possible role of the CS domain.
REFERENCE   7  (bases 1 to 9205)
  AUTHORS   Talmud,P.J., Drenos,F., Shah,S., Shah,T., Palmen,J., Verzilli,C.,
            Gaunt,T.R., Pallas,J., Lovering,R., Li,K., Casas,J.P., Sofat,R.,
            Kumari,M., Rodriguez,S., Johnson,T., Newhouse,S.J., Dominiczak,A.,
            Samani,N.J., Caulfield,M., Sever,P., Stanton,A., Shields,D.C.,
            Padmanabhan,S., Melander,O., Hastie,C., Delles,C., Ebrahim,S.,
            Marmot,M.G., Smith,G.D., Lawlor,D.A., Munroe,P.B., Day,I.N.,
            Kivimaki,M., Whittaker,J., Humphries,S.E. and Hingorani,A.D.
  CONSRTM   ASCOT investigators; NORDIL investigators; BRIGHT Consortium
  TITLE     Gene-centric association signals for lipids and apolipoproteins
            identified via the HumanCVD BeadChip
  JOURNAL   Am J Hum Genet 85 (5), 628-642 (2009)
   PUBMED   19913121
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 9205)
  AUTHORS   Andersen,G., Wegner,L., Rose,C.S., Xie,J., Zhu,H., Larade,K.,
            Johansen,A., Ek,J., Lauenborg,J., Drivsholm,T., Borch-Johnsen,K.,
            Damm,P., Hansen,T., Bunn,H.F. and Pedersen,O.
  TITLE     Variation in NCB5OR: studies of relationships to type 2 diabetes,
            maturity-onset diabetes of the young, and gestational diabetes
            mellitus
  JOURNAL   Diabetes 53 (11), 2992-2997 (2004)
   PUBMED   15504981
  REMARK    GeneRIF: Variaation in the coding region is not a major contributor
            in the pathogenesis of nonautoimmune diabetes.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 9205)
  AUTHORS   Zhu,H., Larade,K., Jackson,T.A., Xie,J., Ladoux,A., Acker,H.,
            Berchner-Pfannschmidt,U., Fandrey,J., Cross,A.R.,
            Lukat-Rodgers,G.S., Rodgers,K.R. and Bunn,H.F.
  TITLE     NCB5OR is a novel soluble NAD(P)H reductase localized in the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 279 (29), 30316-30325 (2004)
   PUBMED   15131110
  REMARK    GeneRIF: NCB5OR is a novel soluble NAD(P)H reductase localized in
            the endoplasmic reticulum
REFERENCE   10 (bases 1 to 9205)
  AUTHORS   Zhu,H., Qiu,H., Yoon,H.W., Huang,S. and Bunn,H.F.
  TITLE     Identification of a cytochrome b-type NAD(P)H oxidoreductase
            ubiquitously expressed in human cells
  JOURNAL   Proc Natl Acad Sci U S A 96 (26), 14742-14747 (1999)
   PUBMED   10611283
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA274558.1, BC025380.1, BC029339.1 and AL034347.23.
            
            On Nov 23, 2018 this sequence version replaced NM_016230.3.
            
            Summary: NCB5OR is a flavohemoprotein that contains functional
            domains found in both cytochrome b5 (CYB5A; MIM 613218) and CYB5
            reductase (CYB5R3; MIM 613213) (Zhu et al., 1999 [PubMed
            10611283]).[supplied by OMIM, Jan 2010].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.168530.1,
                                           SRR1803612.44040.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369681.10/ ENSP00000358695.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 DA274558.1         44-47
            5-2013              BC025380.1         10-2018
            2014-2209           BC029339.1         1224-1419
            2210-9205           AL034347.23        52355-59350         c
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q14.2"
     gene            1..9205
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /note="cytochrome b5 reductase 4"
                     /db_xref="GeneID:51167"
                     /db_xref="HGNC:HGNC:20147"
                     /db_xref="MIM:608343"
     exon            1..169
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..1660
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /EC_number="1.6.2.2"
                     /note="NADPH cytochrome B5 oxidoreductase;
                     flavohemoprotein b5+b5R; cytochrome b-type NAD(P)H
                     oxidoreductase; flavohemoprotein b5/b5R; N-terminal
                     cytochrome b5 and cytochrome b5 oxidoreductase
                     domain-containing protein"
                     /codon_start=1
                     /product="cytochrome b5 reductase 4"
                     /protein_id="NP_057314.2"
                     /db_xref="CCDS:CCDS5000.2"
                     /db_xref="GeneID:51167"
                     /db_xref="HGNC:HGNC:20147"
                     /db_xref="MIM:608343"
                     /translation="MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSG
                     KDLTGLKGRLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGS
                     DGTELFDQVHRWVNYESMLKECLVGRMAIKPAVLKDYREEEKKVLNGMLPKSQVTDTL
                     AKEGPSYPSYDWFQTDSLVTIAIYTKQKDINLDSIIVDHQNDSFRAETIIKDCLYLIH
                     IGLSHEVQEDFSVRVVESVGKIEIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYR
                     KCQLISKEDVTHDTRLFCLMLPPSTHLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLL
                     SEFKEPVLPNNKYIYFLIKIYPTGLFTPELDRLQIGDFVSVSSPEGNFKISKFQELED
                     LFLLAAGTGFTPMVKILNYALTDIPSLRKVKLMFFNKTEDDIIWRSQLEKLAFKDKRL
                     DVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLVCICGPVPFTEQGVRLLHD
                     LNFSKNEIHSFTA"
     exon            170..323
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            324..424
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            425..506
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            507..539
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            540..600
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            601..658
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            659..752
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            753..785
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            786..908
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1049
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1202
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1353
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1440
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1605
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..9205
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2188..2193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /note="hexamer: ATTATA"
     polyA_site      2209
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /note="major polyA site"
     regulatory      7776..7781
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /note="hexamer: ATTAAA"
     polyA_site      7800
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
     regulatory      9186..9191
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
                     /note="hexamer: AATAAA"
     polyA_site      9205
                     /gene="CYB5R4"
                     /gene_synonym="cb5/cb5R; dJ676J13.1; NCB5OR"
ORIGIN      
        1 gggcttggcc tctgcccggc cacagagccg gagctggagg tgctgtcccg tctggcggcg
       61 atccccgggc agggcccggg gccggggttt gaagatgctg aacgtccctt cccagtcttt
      121 cccggccccc aggtcgcagc agcgtgtcgc ctccgggggg cgtagcaagg tacctttaaa
      181 acagggcaga agccttatgg attggattcg actgaccaaa agtggaaagg atctaacggg
      241 attaaaaggc aggttaattg aagtaactga agaagaactt aagaaacaca acaaaaaaga
      301 tgattgttgg atatgcataa gaggtttcgt ttataatgtc agcccttata tggagtatca
      361 tcctggtgga gaagatgaac taatgagagc agcaggatca gatggtactg aactttttga
      421 tcaggttcat cgttgggtca attatgaatc catgctgaaa gaatgcctgg ttggcagaat
      481 ggccattaaa cctgctgttc tgaaagacta tcgtgaggag gaaaagaaag tcttaaatgg
      541 catgcttccc aagagccaag tgacagatac acttgccaaa gaaggtccta gttatccaag
      601 ctatgattgg ttccaaacag actctttagt caccattgcc atatatacta aacagaagga
      661 tatcaattta gactcaataa tagttgatca tcagaatgat tcctttagag cagaaacaat
      721 tattaaggat tgtttatatc ttatacatat tgggctaagc catgaggttc aggaagattt
      781 ttctgtgcgg gttgttgaga gtgtgggaaa aatagagatt gttctacaaa aaaaagagaa
      841 tacttcttgg gactttcttg gccatcccct gaagaatcat aattcactta ttccaaggaa
      901 agatacaggt ttgtactaca gaaagtgcca gttaatttcc aaggaagatg ttactcatga
      961 tacgaggctt ttctgtttga tgctgccacc aagcactcat cttcaagtgc ccattgggca
     1021 acatgtttac ctcaagctac ctattacagg tacagaaata gtaaagccat atacacctgt
     1081 atctggttcc ttactctcag agttcaagga accagttctt cccaacaata aatacatcta
     1141 ctttttgata aaaatctatc ccactggact cttcacacca gagcttgatc gtcttcagat
     1201 tggagatttt gtttctgtaa gcagtcctga gggcaatttt aaaatatcca agttccaaga
     1261 attagaagat ctctttttgt tggcagctgg aacaggcttc acaccaatgg ttaaaatact
     1321 gaattatgct ttgactgata tacccagtct caggaaagtg aagctgatgt tcttcaataa
     1381 aacagaagat gatataattt ggagaagcca attggagaaa ttagcattta aagataaaag
     1441 actggatgtt gaatttgttc tctcagcacc tatttctgaa tggaatggca aacagggaca
     1501 tatttcacca gctcttcttt ctgaattttt gaaaagaaat ttggacaaat ccaaagttct
     1561 cgtctgcatt tgtggaccag tgccatttac agaacaagga gtaaggttgc tgcatgatct
     1621 caacttttcc aaaaatgaga tccatagttt tacagcataa tgaagagctg tcattgtcct
     1681 ttattcaact agtttatcta aatttgtgat tgcttagggt tttttaagag aacatttttg
     1741 tacataacaa aaggttaact agaatccagc cttcagtttc ttaaatgaaa tcaaatgttc
     1801 cttcagtaca ggtaacttct tggctttctt ttgtaccaca acttatttta ctactgatat
     1861 ttgacctgga aagttaatca tggcaacaaa tacatacagg attctttgtt atgaatcaca
     1921 aatttccttg ccatttaaat tatatcactg ttctacaata agcacttgtg ttttatgaca
     1981 tgataaagta aatgatcact tcgatcatgt ttctatgctg taaaatgtct tattagcaat
     2041 acagattaaa ttttaccttg cactgttaac tcaggaaatg atcatttatg tccttgttat
     2101 taatgttaaa acatagaatg ttataactta ttaagtgatc caaacatttt tttgtgtgtg
     2161 tatggcattg atgcagaata gaataaaatt atacttaagt tctttttaat ccttgtggta
     2221 gaaatgtggt tgaatgcaac aatattgggc cagcgtctca aggggaattt taaaaggcag
     2281 caataaaata tattagagac atgacacaca tgcgcctttg gactaggtgt gttaacagag
     2341 cttgagagag ggaaccttga cagctatttt tactcttagc caaaaacaaa gacattagga
     2401 aagagagtga aagtatagtg tctagttcca cagcataaag ttattatttg gtgctttgga
     2461 atgggttgaa ttttaattct ttagtccatc taaatcagct ggaaaaatga ggaacattct
     2521 gccttgaatg gttcttcccc tggttggctg ctttctccac aactggtggt tcagcaggaa
     2581 gccctcttag gttcttgcat gtgggcaact tattttctct tcctgctgct ttgtgcaact
     2641 gtcaaatact ttcacagatt cagatcagca aagggtatca aggatagtca ttgtgagctt
     2701 ttcgtgagga aactgttgaa tgcataggta gctactgtat aagctaacta ggatttttca
     2761 gtcttagtac agctgcaatg ttttcacaga aataggggat tgaaagagtc tccaatttct
     2821 ctgtaaatga ttttgtaaac agctaaatct ttgctgaatg aataatatac tcaagaggaa
     2881 cacaatatta atattcccca aattacagac gcccagcttg tgaagcttcc tatgtgcaaa
     2941 tggctggttg aacactatca ctttttcctg tgcctctgtc ttctgtagcc aattccacca
     3001 tcatcattat tcacaaagac aggaatctag ctgtcctgga ctggtactgt tccctggtag
     3061 gtaacaagaa ctttttctag cttcatgcct tccccctact tatttttttc catctaaggg
     3121 tgaggtgatt tggctctgtg tcctcaccca aatctcctct caaattgtaa ttcccacatg
     3181 tcgagggagg gtcttggtgg gaggtgattg gatcatggga gtgattgccc ccatgctgtt
     3241 ctcatgatgg taagtgagtt ctcatgagat ctgatggttt aaaagtgtgg cacttcctcc
     3301 ccacctcctg ctgtattatg aagaaggtcc ttgcttcttc tttgctttct accataattg
     3361 tacatttcct gaggcctccc cagccatgca gaactgtgag tcaattaaac ctcttttctt
     3421 cataaattac ccagtctcag gtagttcttt atagcagtgt gaaaatagac taatataaaa
     3481 agagactatt taccactttt ttttagttca tttgcgatgt atcaaatcta acttagccaa
     3541 catctttctc atcttttcta agcctcttct tttttctctc tcctttccca agcaacagaa
     3601 tctcctatta gtcgctatat gtaatgcttc ctcattcctc cttttatgtt ttatattttt
     3661 gtttatattt tatttatatt ttgtttatat tttgttatat tttattatat tttgttatat
     3721 ttatattttg tttatatttt aaaacaaatc cataagtgtc tcctgggtaa tctccactga
     3781 catgatttta tcaacttaca tgttaattcc caaatgggag tgactaattt gagaatcagc
     3841 tgagcttcag ttttcaatat ccatttactt cctgcatacc accccaccat ccacctagta
     3901 agccattcta gaactattag gaatcaacat caatgtcccc ttctccagtc tgactcttcc
     3961 ataccagcta accctgtcct atcatccctc agctggatta gtacagtagc ttgataattc
     4021 atcttcatgc tgcagtcctg atctcctaaa attcattctc cattccaggg tattctagaa
     4081 ggacttggag gaaaaaatcc cctaagtaga gcctcagtct tccaatgaat gcaaccaaat
     4141 actgaggagc acagatagga gtatagttta tcactgttat tctcaagcaa ataactcaaa
     4201 gactcctaca tcacatattt gcagtgaaga agaattacgc tcaaagtcac atttgcttgc
     4261 aaattctgct ttttttaaaa aaaagtttat taattttcta ttgctgctag atcaaattac
     4321 tacaaactta gtagcttaaa acaacaccca tttattatct cacagttcca tggcacagaa
     4381 atctaggcac aatatggctc aactgggtcc tctgcttaaa gcctcacaag gctgtaatca
     4441 gattgtcagc tgagctgggc tcttagctag aggctgtaag gggaagtccc tagataagag
     4501 ttctttcagc ctcattaggt agatttcagt tccttgcagt tgtaggactg agttccccat
     4561 ttccttgcta gctgttagct gtgggctagt ctttgtttct agaatttgcc cacattcctt
     4621 ctcatacttt ctgtgtggcc ctcttcagca ttagtggatg aatctcatgc tttcagtctc
     4681 tctgaattcc cttgtgggct tcttcagcat tggtagatga atcttatgct ttcaatctct
     4741 ctgaattccc tttctgccat atctttcttg ccctagcaag agttcactgc ttttaagatc
     4801 tcatgtggtt agattggacc tatccagata tccagggtaa tcttcctatt ttaaaaatct
     4861 ataattttaa ttatagctgt aaagtccctt ttcctatata atatattcaa aggttgcagg
     4921 catttgggtg ttgacatctt tgatgggcac attgtgtcta ccacaggcta tagaaagaac
     4981 agagaaattt gtccaagata agatgattag gttttgtcta gataggggcc cttgctatgg
     5041 gccctcagtt tttaccttaa tgtaacagaa catgagagga gctttgacac ccaccccaaa
     5101 agagtcaact tcattatgct ggcaaaggca tgggtacaac ctgctctgtg atctaccttc
     5161 tgaaccacac aagcttgtcc tgaacgaggt tggggctgag tctgttgata acagaccccc
     5221 atttttgggc agaaaaaaca gattctgtat gatctacagt atttaacatt gtggcaaata
     5281 aattataaag gaaaaatgga atctcaagta gttacagtct cttggtgtct ttcaacattg
     5341 gttttatttt gaagtcattt tcacccagca ttgcaagttt agcagacctc aaaacagaat
     5401 gccaaagtga tcttaaaatt caaaaatgag tttactttct ttgttaaagt tctcttttga
     5461 tgcatatccc ccattcatgg aatggaagca ttatcttggg tgcagcatta cacgtagagt
     5521 taaaatgtgg aaacaaccca aacatcctga tatggtttgg ctctgtgtca ccacccaaat
     5581 ctcatcttga attgtactct cataattccc atgtgttgtg ggaggaaccc agtgggagat
     5641 aatttgaatc atgggggcag tttccctcat actgttctca tggtagtgaa taactctcac
     5701 aagacctggt ggttttatca ggggtttccg cttttgcatc ttactcattt tctcttgcgg
     5761 ccgccatgta agaagtgcct ttcacctcct gccatgattc tgaggcctcc ccagccatgt
     5821 ggaactgtaa gtccaattaa accccttttt ttccccagtc tcaggtacgt cttttatcag
     5881 cagcgtgaaa atggactaat acagtaaatt ggtaccagta gagcaggtgt tgctgcaaag
     5941 ataccgggaa atgtggaagc aactttggaa cttggtaaca ggcagagatt ggaacagttt
     6001 ggagggctca gaagaagaca ggaaaatggg aaagtttgga acttccttga gacttgttga
     6061 atggctttga caaaaatact gataatgata tggacaatga aatctagact gaggtggtct
     6121 caggtggaga tgaggaacct gttgagaact ggagcaaagg tgactcttgt tatgatttag
     6181 caaagagact ggcggcattt tgcccctgcc ctggagattt gtggaactct gaacttgaga
     6241 gagatgatta agggtatctg gtggaagaaa tttctaagca gcaaagcatt caagaggtga
     6301 ctcaagtgtt gttaaaggca ttcagtttta aaagggaagc agagcattaa aattcagaaa
     6361 attagcagct tgacaatgcg atagaaaaga aaatcctatt ttctgaggag aaattcaagc
     6421 tggctgtaga tatttgcata agtaacaagg aactgaatgt taatttccaa gacaatgggg
     6481 aaaatgtctc cagggcatgt cagagacatt tgtggcagcc tcttctatca cagacctgga
     6541 ggtctaggaa gaaaaaatgg taaaaatggt tttgtgggca aggcctaggg tccttgtgct
     6601 gtgtgcagtc taaggacttg gtgccctgtt tcctagccac tccggccatg gctgaaaggg
     6661 gccaacatga agctcaggcc atggcttcag agggtgaaag ccttaagcct tggcaacttc
     6721 catgtgatgt tgagcatgtg ggtgcacaga agtcaagaaa tggggtttgg gaacctctgt
     6781 ctagatttca gaagatgtat ggaaatgcct ggatgcccag gcagaagttt gctgcagggg
     6841 cggggctctc atggagaacc tcggctaggg cagtgcagaa gggaaatgtg gggttggagc
     6901 cccctcacag agtccctact gggacaccgc ctagtggagc tgtgagaaga ggaccactgt
     6961 cctccagacc ccagaatggt agatcgactt acagcttgta ccaggtgcct ggaaaagctg
     7021 cagatactca acaccagccc atgaaagcag ccaggatgga ggctgtaccc tgaaagccac
     7081 agggccagag ctgcccaaga ccatgggaag ccacctcttg catcagcgtg acctggatgt
     7141 gagacctgga gtaaaaggag atcattttgg agctttaaaa tttgactgcc ccactggatt
     7201 ttggacttcc atgggccctg taaccccttt gttttggcca atttctccca tttggaatgg
     7261 ctgtatttac ccaatacctg taccctcatt gtatctagga agtaactagc ttgcttttga
     7321 ttttacaggc tcataagtgg aagggacttg ccttgtctca gatgagactt ttgaactgtg
     7381 gacttttggg ttaatgctga aatgatttaa gactttgggg tactgttggg aatgcatgat
     7441 tggttttgaa atgtgaggac ataagatttg gaggagccag gggtgggatg atatggtttg
     7501 gctctgtgtc cccacccaaa tttcatcttg aattatactc ccataattcc catgtgttat
     7561 acgtgggacc tggtgggaga taatttgaat catgggggtg gtttccccca tactgttctc
     7621 atggtagaga ataagtctca tgagatctga tggtttcatc agggggttcc gcttttgcat
     7681 cttactcatt tctcttgctg ccaccctgta agaagtattt ttaacctacc gccatgattc
     7741 tgaggcctcc ccagccatgt ggaactataa gtccaattaa acctcttttt cttggcttaa
     7801 tttcttgggt atgtctttat cagcagtgat tctattccta tgaaatgtct agaacaggaa
     7861 aatctatgag acataaagta attaagtggc tgttcagggg atacaggaat aggggataat
     7921 aactaaaggg ttgggagggt gtttttgaaa tgctaaaata ttctgaagtt tactgtggtt
     7981 atggttgcac atacttatga atatacctaa aaatgttgaa ttgtacattt taagtagatg
     8041 aattgtatct aatttgaacc atatctcagt aaagatataa aaatgttttt gggtactaag
     8101 actaaactag aaagaacata agaggaaata catattatat aagaagaaaa gagtaaaaat
     8161 aaatctttaa aaaaataaaa ataaaaagga ggcctgtgtg cggtggctca tgcctgtaat
     8221 cccagcactt tgggaggccg aggcgggtgg atcacctgag gtcgggagtt tgagaccagc
     8281 ctgaccaaca tggagaaacc ctctctctac taaaaataca aaattagcca ggtgtggtag
     8341 cataggcctg taatccctgc tacttgggag gctgaggcag gataattgcc tgaacccggg
     8401 aggcggaggt tgcggtgagc cgagattgca cctttgcact ccagcctggg caacaagagt
     8461 gaaactccat ctcaaaaaat aaaacaaata aaaaggattc tagaggaagt ggtaaatagt
     8521 gaagataggc aaagaatatc caacatatga ataatagtag tccttgaaga caaaaatcaa
     8581 aggaaaagaa tactaaaaac tctacttaat gaaaaatcat cctgaaatta acaagaaaaa
     8641 ttgaaattaa tgttgaaaga gcaccagaga gtatacatcc aaaaagacca acattaatat
     8701 aaattttagt aaaattactg aaccatgaaa agtaaatgcc tttagttatc taaacaaaaa
     8761 ctaattactt aaaagggaaa gaaaattaga tcatcaccag acttttcaac agcattgcct
     8821 tatgctttaa gaaagtgttg taccacattt gatatcctga tggaaaaaaa tgtgagccaa
     8881 ggattttaca cccaacaaaa cttaatggtg aatataaagg cataaaccaa tatagaatgc
     8941 aaaaacttgg gaaatattgt tttccaaagc ctttcctaag taatctacaa aagaacaagc
     9001 tttaaacaac cagaaagact agaaagtcat caataaaagg attggagaac attaaacttg
     9061 tagaactaaa acaaaatgag agttaaaaaa gaaagagtat aggtatatgc taaactattt
     9121 cagtagtttt ggcagtggtt gtattagtat tgttattctg aagatgtgaa ataatgcaaa
     9181 cgcataataa aaaatgtcat ctggt
//
