LOCUS       NM_153611               2584 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens cytochrome b561 family member A3 (CYB561A3),
            transcript variant 2, mRNA.
ACCESSION   NM_153611
VERSION     NM_153611.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Wang,Z., Guo,R., Trudeau,S.J., Wolinsky,E., Ast,T., Liang,J.H.,
            Jiang,C., Ma,Y., Teng,M., Mootha,V.K. and Gewurz,B.E.
  TITLE     CYB561A3 is the key lysosomal iron reductase required for Burkitt
            B-cell growth and survival
  JOURNAL   Blood 138 (22), 2216-2230 (2021)
   PUBMED   34232987
  REMARK    GeneRIF: CYB561A3 is the key lysosomal iron reductase required for
            Burkitt B-cell growth and survival.
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2584)
  AUTHORS   Asard,H., Barbaro,R., Trost,P. and Berczi,A.
  TITLE     Cytochromes b561: ascorbate-mediated trans-membrane electron
            transport
  JOURNAL   Antioxid Redox Signal 19 (9), 1026-1035 (2013)
   PUBMED   23249217
  REMARK    Review article
REFERENCE   4  (bases 1 to 2584)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP003108.3.
            
            On Dec 3, 2019 this sequence version replaced NM_153611.5.
            
            Transcript Variant: This variant (2) uses an alternate splice
            junction at the 5' end of the last exon compared to variant 4. The
            resulting isoform (2) has a shorter and distinct C-terminus
            compared to isoform 3. Variants 2 and 3 both encode the same
            isoform (2).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014045.2, AK056751.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000294072.9/ ENSP00000294072.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-150               AP003108.3         33921-34070         c
            151-246             AP003108.3         29921-30016         c
            247-445             AP003108.3         28718-28916         c
            446-654             AP003108.3         25972-26180         c
            655-809             AP003108.3         25173-25327         c
            810-966             AP003108.3         23179-23335         c
            967-2584            AP003108.3         20993-22610         c
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.2"
     gene            1..2584
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /note="cytochrome b561 family member A3"
                     /db_xref="GeneID:220002"
                     /db_xref="HGNC:HGNC:23014"
                     /db_xref="MIM:618757"
     exon            1..150
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /inference="alignment:Splign:2.1.0"
     exon            151..246
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /inference="alignment:Splign:2.1.0"
     exon            247..445
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..990
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /EC_number="7.2.1.3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     lysosomal cytochrome b; cytochrome b ascorbate-dependent
                     protein 3; cytochrome b, ascorbate dependent 3; lysosomal
                     membrane ascorbate-dependent ferrireductase CYB561A3;
                     lysosomal cytochrome b561"
                     /codon_start=1
                     /product="lysosomal membrane ascorbate-dependent
                     ferrireductase CYB561A3 isoform 2"
                     /protein_id="NP_705839.3"
                     /db_xref="CCDS:CCDS8004.1"
                     /db_xref="GeneID:220002"
                     /db_xref="HGNC:HGNC:23014"
                     /db_xref="MIM:618757"
                     /translation="MVSGRFYLSCLLLGSLGSMCILFTIYWMQYWRGGFAWNGSIYMF
                     NWHPVLMVAGMVVFYGGASLVYRLPQSWVGPKLPWKLLHAALHLMAFVLTVVGLVAVF
                     TFHNHGRTANLYSLHSWLGITTVFLFACQWFLGFAVFLLPWASMWLRSLLKPIHVFFG
                     AAILSLSIASVISGINEKLFFSLKNTTRPYHSLPSEAVFANSTGMLVVAFGLLVLYIL
                     LASSWKRPEPGILTDRQPLLHDGE"
     exon            446..654
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /inference="alignment:Splign:2.1.0"
     exon            655..809
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /inference="alignment:Splign:2.1.0"
     exon            810..966
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /inference="alignment:Splign:2.1.0"
     exon            967..2584
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2563..2568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /note="hexamer: ATTAAA"
     polyA_site      2584
                     /gene="CYB561A3"
                     /gene_synonym="CYBASC3; LCYTB"
                     /note="major polyA site"
ORIGIN      
        1 gctctggggc ggcattgcca gccggctgta ggcattcagg gcagtgtctt ctgcatctcc
       61 taggaacctc gggagcggca gctccggcgc ctggtagcga gaggcgggtt ccggagatcc
      121 cggcctcact tcgtcccact gtggttaggg gtgagtcctg caaatgttaa gtgatttgct
      181 caaggtgccc atttcgcagg aattggagcc caggccagtt ctctgagcct atcattaggg
      241 ctaaaggagt gcgtgatcag aatggtgtct ggacggttct acttgtcctg cctgctgctg
      301 gggtccctgg gctctatgtg catcctcttc actatctact ggatgcagta ctggcgtggt
      361 ggctttgcct ggaatggcag catctacatg ttcaactggc acccagtgct tatggttgct
      421 ggcatggtgg tattctatgg aggtgcgtca ctggtgtacc gcctgcccca gtcgtgggtg
      481 gggcccaaac tgccctggaa actcctccat gcagcgctgc acctgatggc cttcgtcctc
      541 actgttgtgg ggctggttgc tgtctttacg tttcacaacc atggaaggac tgccaacctc
      601 tactcccttc acagctggct gggcatcacc actgtcttcc tcttcgcctg ccagtggttc
      661 ctgggctttg ctgtcttcct cctgccctgg gcgtccatgt ggctgcgcag cctcctaaaa
      721 cctatccacg tcttttttgg agccgccatc ctctctctgt ccatcgcatc cgtcatttcg
      781 ggcattaatg agaagctttt cttcagtttg aaaaacacca ccaggccata ccacagcctg
      841 cccagtgagg cggtctttgc caacagcacc gggatgctgg tggtggcctt tgggctgctg
      901 gtgctctaca tccttctggc ttcatcttgg aagcgcccag agccggggat cctgaccgac
      961 agacagcccc tgctgcatga tggggagtga agcagcagga aggggctccc aagagctcct
     1021 ggtggtgcag cctgtgctcc cctcagaagc tctgctcttc ccagggctcc cggctggttt
     1081 cagcaggcga ctttcttcca atgctgggcc cagacttctt gcctgggtgc tggcctgccc
     1141 tctccggccg cttgctgcct gtctgctttc cttggtggct ttgcctgggt gctgggcctg
     1201 ccctctccgg ccgcttgctg cctgtctgct ttccttggtg gctttgcctg ggtgctgggc
     1261 ctgccttctc tggctgcttg ctgcctgtct gctttccttg gtggctttgg cttctgcact
     1321 ccttggcgtc agcctctcag gtcctccatt cacacgaggt cctcctcgct ctggccgctc
     1381 ttgctgctcc tgtctgaaga aatcagactg atttcctctt aagactccta gggatgtggt
     1441 gaagagctgg gactcaagtg cagtccacgg tgtgaaacat gagggaggtg aggtgtccgt
     1501 ccacttcccc cataaaggtg tgcatttcag ttaggctgcc ccgccacaga gcaggcttca
     1561 tctgctctgc catccagccc catctggatg tgaggtgggg tggagacatc atggggtgat
     1621 tgcagaaagg gggagtggcg gcccacgcag cttctgctga ggagctgacc gctctgagct
     1681 gttctgtttc gtattgctgc tctgtgtctg catgtattgt gaccgtgcgg ctccacctct
     1741 tccagctgct gctacagctg aggcctggat cccggccttt ccctgtgact tacgtgtctg
     1801 tcaccggcag gcagccctac aaatcctggt gacctgctct cccaagaaca gagcctgtcc
     1861 ccagatgtcc cagtagcgat gagtaacaga ggtggctgtg gacttcctct acttctcctt
     1921 gctggatcag ggccttcctg cctcccgctg ggcaggtctg gccttgctct cttggcaggg
     1981 ccccagcccc tctgaccact ctgcagctca ccatgcagct gatgccaaag ttgtggtgtc
     2041 cagtgtgcag cagccctggg agccactgcc accttcagag gggttccttg ctgagaccca
     2101 cattgcttca cctggcccca ccatggctgc ttgcctggcc caacctagcg ttctgtgcca
     2161 tgctagagct tgagctgttg ctcttcttca ggggaggaaa tagggtggag agcgggaagg
     2221 gtcttgctcc taagtgttgc tgctgtggct tttttgcctt ctccaaagac gcactgccag
     2281 gtcccaagct tcagactgct gtgcttagta agcaagtgag aagcctgggg tttggagccc
     2341 acctactctc tggcagcatc agcatcctac tcctggcaac atcaggccaa cgtccacccc
     2401 agcctcacat tgccagatgt tggcagaagg gctaatattg accgtcttga ctggctggag
     2461 ccttcaaagc cactgggatg tcctccaggc acctgggtcc catgaccagc tccccatctc
     2521 cataggggta ggcatttcac tggtttatga agctcgagtt tcattaaata tgttaagaat
     2581 caaa
//
