LOCUS       NM_001915               2929 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens cytochrome b561 (CYB561), transcript variant 1, mRNA.
ACCESSION   NM_001915
VERSION     NM_001915.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Zhuo,J., Zhao,Y., Hao,R., Li,H., Zheng,Z., Dai,L., Sheng,A.,
            Yao,H., Tang,Y., Wang,R., Yang,X. and Liu,W.
  TITLE     CYB561 is a potential therapeutic target for breast cancer and is
            associated with immune cell infiltration
  JOURNAL   Eur J Med Res 29 (1), 414 (2024)
   PUBMED   39135107
  REMARK    GeneRIF: CYB561 is a potential therapeutic target for breast cancer
            and is associated with immune cell infiltration.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Ge,Y., Hong,M., Zhang,Y., Wang,J., Li,L., Zhu,H., Sheng,Y., Wu,W.S.
            and Zhang,Z.
  TITLE     miR-30e-5p regulates leukemia stem cell self-renewal through the
            Cyb561/ROS signaling pathway
  JOURNAL   Haematologica 109 (2), 411-421 (2024)
   PUBMED   37584287
  REMARK    GeneRIF: miR-30e-5p regulates leukemia stem cell self-renewal
            through the Cyb561/ROS signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2929)
  AUTHORS   Holst,J.D., Murphy,L.G., Gorman,M.J. and Ragan,E.J.
  TITLE     Comparison of insect and human cytochrome b561 proteins: Insights
            into candidate ferric reductases in insects
  JOURNAL   PLoS One 18 (12), e0291564 (2023)
   PUBMED   38039324
  REMARK    GeneRIF: Comparison of insect and human cytochrome b561 proteins:
            Insights into candidate ferric reductases in insects.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2929)
  AUTHORS   Zhou,X., Guo,X., Han,J., Wang,M., Liu,Z., Ren,D., Zhao,J. and Li,Z.
  TITLE     Cytochrome b561 regulates iron metabolism by activating the
            Akt/mTOR pathway to promote Breast Cancer Cells proliferation
  JOURNAL   Exp Cell Res 431 (1), 113760 (2023)
   PUBMED   37634562
  REMARK    GeneRIF: Cytochrome b561 regulates iron metabolism by activating
            the Akt/mTOR pathway to promote Breast Cancer Cells proliferation.
REFERENCE   5  (bases 1 to 2929)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2929)
  AUTHORS   Bashtovyy,D., Berczi,A., Asard,H. and Pali,T.
  TITLE     Structure prediction for the di-heme cytochrome b561 protein family
  JOURNAL   Protoplasma 221 (1-2), 31-40 (2003)
   PUBMED   12768339
  REMARK    GeneRIF: structural features in the cyt b(561) family are well
            conserved at both the sequence and the protein level
REFERENCE   7  (bases 1 to 2929)
  AUTHORS   Srivastava,M.
  TITLE     Genomic structure and expression of the human gene encoding
            cytochrome b561, an integral protein of the chromaffin granule
            membrane
  JOURNAL   J Biol Chem 270 (39), 22714-22720 (1995)
   PUBMED   7559396
REFERENCE   8  (bases 1 to 2929)
  AUTHORS   Srivastava,M., Gibson,K.R., Pollard,H.B. and Fleming,P.J.
  TITLE     Human cytochrome b561: a revised hypothesis for conformation in
            membranes which reconciles sequence and functional information
  JOURNAL   Biochem J 303 (Pt 3) (Pt 3), 915-921 (1994)
   PUBMED   7980462
REFERENCE   9  (bases 1 to 2929)
  AUTHORS   McBride,O.W., Yi,H.F. and Srivastava,M.
  TITLE     The human cytochrome b561 gene (CYB561) is located at 17q11-qter
  JOURNAL   Genomics 21 (3), 662-663 (1994)
   PUBMED   7959749
REFERENCE   10 (bases 1 to 2929)
  AUTHORS   Duong,L.T. and Fleming,P.J.
  TITLE     Isolation and properties of cytochrome b561 from bovine adrenal
            chromaffin granules
  JOURNAL   J Biol Chem 257 (15), 8561-8564 (1982)
   PUBMED   7096323
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM552101.1, BC002976.1 and BC091485.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001915.3.
            
            Transcript Variant: This variant (1) represents the shortest
            transcript. Variants 1-3 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.265222.1,
                                           SRR1803613.251353.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2156266 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360793.8/ ENSP00000354028.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                BM552101.1         56-146
            92-2168             BC002976.1         104-2180
            2169-2929           BC091485.1         2195-2955
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.3"
     gene            1..2929
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /note="cytochrome b561"
                     /db_xref="GeneID:1534"
                     /db_xref="HGNC:HGNC:2571"
                     /db_xref="MIM:600019"
     exon            1..62
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /inference="alignment:Splign:2.1.0"
     exon            63..277
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..831
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ferric-chelate reductase 2; cytochrome b-561; cytochrome
                     b561 family, member A1; transmembrane ascorbate-dependent
                     reductase CYB561; chromaffin granule CYB561"
                     /codon_start=1
                     /product="transmembrane ascorbate-dependent reductase
                     CYB561 isoform 1"
                     /protein_id="NP_001906.3"
                     /db_xref="CCDS:CCDS11636.1"
                     /db_xref="GeneID:1534"
                     /db_xref="HGNC:HGNC:2571"
                     /db_xref="MIM:600019"
                     /translation="MEGGAAAATPTALPYYVAFSQLLGLTLVAMTGAWLGLYRGGIAW
                     ESDLQFNAHPLCMVIGLIFLQGNALLVYRVFRNEAKRTTKVLHGLLHIFALVIALVGL
                     VAVFDYHRKKGYADLYSLHSWCGILVFVLYFVQWLVGFSFFLFPGASFSLRSRYRPQH
                     IFFGATIFLLSVGTALLGLKEALLFNLGGKYSAFEPEGVLANVLGLLLACFGGAVLYI
                     LTRADWKRPSQAEEQALSMDFKTLTEGDSPGSQ"
     exon            278..376
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /inference="alignment:Splign:2.1.0"
     exon            377..480
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /inference="alignment:Splign:2.1.0"
     exon            481..638
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /inference="alignment:Splign:2.1.0"
     exon            639..2929
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2911..2916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /note="hexamer: AATAAA"
     polyA_site      2929
                     /gene="CYB561"
                     /gene_synonym="CGCytb; CYB561A1; FRRS2; ORTHYP2"
                     /note="major polyA site"
ORIGIN      
        1 agaaccggcc aagcgacccg ggcgcggcgc ggggaggctg aagggacgct cgggtaggca
       61 agcgtttgcc tcagcatgga gggcggggcc gcggcagcca cccccacagc actgccttac
      121 tacgtggcct tctcccagct gctgggcctg accttggtgg ccatgaccgg cgcgtggctc
      181 gggctgtacc gaggcggcat tgcctgggag agcgacctgc agttcaacgc gcaccccctc
      241 tgcatggtca taggcctgat cttcctgcag ggaaatgccc tgctggttta ccgtgtcttc
      301 aggaacgaag ctaaacgcac caccaaggtc ctgcacgggc tgctgcacat ctttgcgctc
      361 gtcatcgccc tggttggctt ggtggcggtg ttcgactacc acaggaagaa gggctacgct
      421 gacctgtaca gcctacacag ctggtgcggg atccttgtct ttgtcctgta ctttgtgcag
      481 tggctggtgg gcttcagctt cttcctgttc cccggagctt cattctccct gcggagccgc
      541 taccgcccac agcacatctt ctttggtgct accatcttcc tcctttccgt gggcaccgcc
      601 ctgctgggcc tgaaggaggc actgctgttc aacctcgggg gcaagtatag cgcatttgag
      661 cccgagggtg tcctggccaa cgtgctgggc ctgctgctgg cctgcttcgg tggggcggtg
      721 ctctacatct tgacccgggc cgactggaag cggccttccc aggcggaaga gcaggccctc
      781 tccatggact tcaagacgct gacggaggga gatagccccg gctcccagtg atgcgcccgg
      841 ccggccctgg gggttcgcgg ggtgtcttct tgcctgcccc tgctgaggcg tcttcaggac
      901 tgcaggctcc ggagagtggc tctggcagca ggcgggcgcg tgggtgcagc tgcatctgtt
      961 tgagtgctgc tttctggggt caggtctccg cctcctctgc ttctcctttc tccgctgcta
     1021 tagaccagtt cattgtgtgt ggctcccgtg tctctgttgc ccccttcagt gcagaaggct
     1081 ttgggtagga cttcgggtgt tcggtcctgg tcgcagagca cagatcttta aagaagcgag
     1141 agaggaggcc ccaccctcct ggcagcagat gcctggggca aggccagggg aaactggggg
     1201 ggcctcaggg acaggcctgg aaaggccacg atggctgctg aattcaaaca aggagtccct
     1261 ccagcctgaa taacacgtgg cacaaatggg cccggccttt ggcagaggag caagtgatat
     1321 gatgtgtaaa gtatgttggt ggtgaaagca aggttcccca ggagagggga gggactggcc
     1381 cctgggaagc tctgagatga ggctgtggcc cagctgtagt cctgaccttc ctcttcttta
     1441 accctttagc cctaggatgg ctttggtggg agaggggata gaagcccatg acttcagaca
     1501 gactttctct tggcagatgc aggcgggcct cctcccaggc tgctccagac atgggggttg
     1561 gggatggggg gcaccttgca gccccttcct gctggggctc cctccttgta gcacccccct
     1621 tgcggctcag ctctggtttc ctctcccagg ctcacccagg ctctgctcag gctgggaggc
     1681 agagggcaca aaccttataa ttttttaaat gaaaaaccgc tgctgctggc tgtggctaga
     1741 gccccctggg gctgctggag ctgctgcctc tgttctggag gacgagcctt ctccttatct
     1801 gctgcccatc tttccaggaa gtcaggatgg agtcagaaca actacagtca tcccccgtgg
     1861 tgtctgcaca tcactccagc cccataaaga gtgtcatgtt agctgagtca ccatttggct
     1921 tcggcctgga aatagtgtga ttagaacact gatcgtgtgc gaggccagga gatcaagacc
     1981 atcctgacta acaaacacag tgaaaccccg tctctactaa aaatacaaaa aaattagcca
     2041 ggcgtggtgg tgggcgcctg tagtcccagc tacttgggag gctgaggcag gagaatggtg
     2101 tgaacccggg agatggcgct tgcagtgagc tgagattgca ctccagcctg ggcgacaggc
     2161 tcaaaaaaga aaaaaaaaag aacaccgatc atgtgcttct tggatctggt gactgttctc
     2221 tccctgtttt cctttctttt tgggtgtttg aggagcatgg cttagcttga gacacacaca
     2281 gacactgtgt acttcagtga agggcttaat atacagtttc caaacctgac gactcttctt
     2341 cttgtaatgg ctgccctttt cctacctgag gccgtcttag agaaaggggc cagtctcctc
     2401 taatgctcag atttcccata gttggctttt gctgtgtctc ctgcctcagg cagtgtcatt
     2461 tctgggagca ggtggttgta gtccaggccc ctccccagca gggtctgccc aggctccttc
     2521 gagccccttt ccccgcctcc tctcagcctg tccggatgac agtgttcgcc tcctgtttag
     2581 actgtacact cttcaggggt aggggtgccg tcagttcttc aatcagctgg cacacacttg
     2641 tatagtgaaa tgtttacatg tgggaaaact ccgccttaga caaactacca aagtacaatc
     2701 gtgtctctct ctagccggaa tgctacagag agaaatggaa ccttagattt gcaacaaaag
     2761 tctgtaaact ggtctgtttg ccaaagtgaa cactggatga ctaaggagct gaagaaggcc
     2821 cccagaagcg gatttgtggt gggttatttt attttgcctg tggccaatct tctgtgaaat
     2881 acaatgtgct gttggtgcaa cagatgattc aataaatgtc tacagcaga
//
