LOCUS       NM_001025265             834 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens chromosome X open reading frame 65 (CXorf65),
            transcript variant 1, mRNA.
ACCESSION   NM_001025265 XM_088684
VERSION     NM_001025265.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   He,Y., Xu,R., Zhai,B., Zhou,S., Wang,X. and Wang,R.
  TITLE     Gm614 Protects Germinal Center B Cells From Death by Suppressing
            Caspase-1 Transcription in Lupus-Prone Mice
  JOURNAL   Front Immunol 11, 585726 (2020)
   PUBMED   33193409
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB457782.1, DB523273.1 and AL590764.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001025265.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.166161.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374251.6/ ENSP00000363369.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-268               DB457782.1         5-272
            269-699             DB523273.1         1-431
            700-700             AL590764.3         109789-109789       c
            701-733             DB523273.1         433-465
            734-834             AL590764.3         109655-109755       c
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.1"
     gene            1..834
                     /gene="CXorf65"
                     /note="chromosome X open reading frame 65"
                     /db_xref="GeneID:158830"
                     /db_xref="HGNC:HGNC:33713"
     exon            1..209
                     /gene="CXorf65"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..736
                     /gene="CXorf65"
                     /codon_start=1
                     /product="uncharacterized protein CXorf65"
                     /protein_id="NP_001020436.1"
                     /db_xref="CCDS:CCDS35324.1"
                     /db_xref="GeneID:158830"
                     /db_xref="HGNC:HGNC:33713"
                     /translation="MFIFIKHGDNQQFLVNTNCAVVVLLYYIRSKVKLPKTNTIDLCE
                     QTGKMKMLFLMKPNHAEYASKYLTARSTYYVCKVERGPPGTRLENAYRAFVPLLKNPE
                     PWLLVALRIQCDALERRRIQMLKMKEAKKVVIIEPPASVPSKQSGRSDKKKSTRKSPT
                     FRNRPDFRKNKGRQLNKTTKQKK"
     exon            210..296
                     /gene="CXorf65"
                     /inference="alignment:Splign:2.1.0"
     exon            297..434
                     /gene="CXorf65"
                     /inference="alignment:Splign:2.1.0"
     exon            435..503
                     /gene="CXorf65"
                     /inference="alignment:Splign:2.1.0"
     exon            504..610
                     /gene="CXorf65"
                     /inference="alignment:Splign:2.1.0"
     exon            611..834
                     /gene="CXorf65"
                     /inference="alignment:Splign:2.1.0"
     regulatory      710..715
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CXorf65"
                     /note="hexamer: AATAAA"
     polyA_site      736
                     /gene="CXorf65"
                     /note="major polyA site"
ORIGIN      
        1 atccacaact atcaacagct ctctcctgcc tgtccagcct gggcatcctc agcctatgaa
       61 atccatctgc ctctctagtc caccaaccct ataggcctat ttgccccaca caaggcctct
      121 agaacaccct ccttctgcat gactcatcag ggcactttgt gaagagtgag gcgatcttga
      181 gggcatgttc atcttcatca aacatggaga taatcagcag tttctggtca ataccaactg
      241 tgctgtcgtc gtgttgctgt attacatccg cagtaaagta aagttgccta aaacaaacac
      301 catcgatttg tgtgaacaaa cggggaaaat gaagatgctt ttcctgatga agcccaatca
      361 tgcagagtat gccagcaaat accttacagc tcgaagcacc tactacgttt gtaaggtgga
      421 acgtgggcca ccaggaacca ggctggagaa tgcttacaga gcttttgtgc ctctcctcaa
      481 gaatccggag ccttggctgc ttgttgcact gcgcatacaa tgtgatgccc tggagaggag
      541 acgaattcag atgcttaaaa tgaaagaagc caagaaggtc gttataattg aaccccctgc
      601 gagtgtcccg tccaagcaat caggacgatc agacaaaaag aagtccactc gcaagtctcc
      661 gacctttagg aacagaccag acttcaggaa gaataaaggt cgccaactca ataaaaccac
      721 caagcaaaag aagtaatact aggtgtgaaa ttactggaat ttaacgtgag atttcttttt
      781 ctagaaagac tccttctccc aatatctgca ctaaggcttg agtacatacg cccc
//
