LOCUS       NM_000584               1642 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens C-X-C motif chemokine ligand 8 (CXCL8), transcript
            variant 1, mRNA.
ACCESSION   NM_000584
VERSION     NM_000584.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Si,W., Liu,J., Wang,Y., Mao,Y., Zhang,Y., Xu,S., Guo,K., Zhang,Y.,
            Hu,Y. and Zhang,F.
  TITLE     IL-8 promotes lens capsular residual cells migration by
            down-regulates expression of E-cadherin and ZO-1 via the
            CXCR1/2-NF-kappaB-RhoA signal pathway
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113074 (2024)
   PUBMED   39244903
  REMARK    GeneRIF: IL-8 promotes lens capsular residual cells migration by
            down-regulates expression of E-cadherin and ZO-1 via the
            CXCR1/2-NF-kappaB-RhoA signal pathway.
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Dhurua,S., Maity,S., Maity,B. and Jana,M.
  TITLE     Comparative Bindings of Glycosaminoglycans with CXCL8 Monomer and
            Dimer: Insights from Conformational Dynamics and Kinetics of
            Hydrogen Bonds
  JOURNAL   J Phys Chem B 128 (42), 10348-10362 (2024)
   PUBMED   39405497
  REMARK    GeneRIF: Comparative Bindings of Glycosaminoglycans with CXCL8
            Monomer and Dimer: Insights from Conformational Dynamics and
            Kinetics of Hydrogen Bonds.
REFERENCE   3  (bases 1 to 1642)
  AUTHORS   Carpi,S., Polini,B., Nieri,D., Doccini,S., Conti,M., Bazzan,E.,
            Pagnini,M., Santorelli,F.M., Cecchini,M., Nieri,P., Celi,A. and
            Neri,T.
  TITLE     Extracellular Vesicles Induce Nuclear Factor-kappaB Activation and
            Interleukin-8 Synthesis through miRNA-191-5p Contributing to
            Inflammatory Processes: Potential Implications in the Pathogenesis
            of Chronic Obstructive Pulmonary Disease
  JOURNAL   Biomolecules 14 (8), 1030 (2024)
   PUBMED   39199417
  REMARK    GeneRIF: Extracellular Vesicles Induce Nuclear Factor-kappaB
            Activation and Interleukin-8 Synthesis through miRNA-191-5p
            Contributing to Inflammatory Processes: Potential Implications in
            the Pathogenesis of Chronic Obstructive Pulmonary Disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1642)
  AUTHORS   Asiri,A., Hazeldine,J., Moiemen,N. and Harrison,P.
  TITLE     IL-8 Induces Neutrophil Extracellular Trap Formation in Severe
            Thermal Injury
  JOURNAL   Int J Mol Sci 25 (13), 7216 (2024)
   PUBMED   39000323
  REMARK    GeneRIF: IL-8 Induces Neutrophil Extracellular Trap Formation in
            Severe Thermal Injury.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1642)
  AUTHORS   Vukolova,Y., Gubareva,I., Galati,G. and Germanova,O.
  TITLE     Diagnostic utility of sortilin & other biomarkers in the diagnosis
            of carotid & coronary atherosclerosis in individuals with arterial
            hypertension
  JOURNAL   Indian J Med Res 159 (5), 511-518 (2024)
   PUBMED   39382416
  REMARK    GeneRIF: Diagnostic utility of sortilin & other biomarkers in the
            diagnosis of carotid & coronary atherosclerosis in individuals with
            arterial hypertension.
REFERENCE   6  (bases 1 to 1642)
  AUTHORS   Koch,A.E., Polverini,P.J., Kunkel,S.L., Harlow,L.A., DiPietro,L.A.,
            Elner,V.M., Elner,S.G. and Strieter,R.M.
  TITLE     Interleukin-8 as a macrophage-derived mediator of angiogenesis
  JOURNAL   Science 258 (5089), 1798-1801 (1992)
   PUBMED   1281554
REFERENCE   7  (bases 1 to 1642)
  AUTHORS   Morris,S.W., Nelson,N., Valentine,M.B., Shapiro,D.N., Look,A.T.,
            Kozlosky,C.J., Beckmann,M.P. and Cerretti,D.P.
  TITLE     Assignment of the genes encoding human interleukin-8 receptor types
            1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35
  JOURNAL   Genomics 14 (3), 685-691 (1992)
   PUBMED   1427896
REFERENCE   8  (bases 1 to 1642)
  AUTHORS   Shimoya,K., Matsuzaki,N., Taniguchi,T., Kameda,T., Koyama,M.,
            Neki,R., Saji,F. and Tanizawa,O.
  TITLE     Human placenta constitutively produces interleukin-8 during
            pregnancy and enhances its production in intrauterine infection
  JOURNAL   Biol Reprod 47 (2), 220-226 (1992)
   PUBMED   1391327
REFERENCE   9  (bases 1 to 1642)
  AUTHORS   Baggiolini,M. and Clark-Lewis,I.
  TITLE     Interleukin-8, a chemotactic and inflammatory cytokine
  JOURNAL   FEBS Lett 307 (1), 97-101 (1992)
   PUBMED   1639201
  REMARK    Review article
REFERENCE   10 (bases 1 to 1642)
  AUTHORS   Schall,T.J., Bacon,K., Toy,K.J. and Goeddel,D.V.
  TITLE     Selective attraction of monocytes and T lymphocytes of the memory
            phenotype by cytokine RANTES
  JOURNAL   Nature 347 (6294), 669-671 (1990)
   PUBMED   1699135
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG497712.1 and BC013615.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000584.3.
            
            Summary: The protein encoded by this gene is a member of the CXC
            chemokine family and is a major mediator of the inflammatory
            response. The encoded protein is commonly referred to as
            interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages,
            neutrophils, eosinophils, T lymphocytes, epithelial cells, and
            fibroblasts. It functions as a chemotactic factor by guiding the
            neutrophils to the site of infection. Bacterial and viral products
            rapidly induce IL-8 expression. IL-8 also participates with other
            cytokines in the proinflammatory signaling cascade and plays a role
            in systemic inflammatory response syndrome (SIRS). This gene is
            believed to play a role in the pathogenesis of the lower
            respiratory tract infection bronchiolitis, a common respiratory
            tract disease caused by the respiratory syncytial virus (RSV). The
            overproduction of this proinflammatory protein is thought to cause
            the lung inflammation associated with csytic fibrosis. This
            proinflammatory protein is also suspected of playing a role in
            coronary artery disease and endothelial dysfunction. This protein
            is also secreted by tumor cells and promotes tumor migration,
            invasion, angiogenesis and metastasis. This chemokine is also a
            potent angiogenic factor. The binding of IL-8 to one of its
            receptors (IL-8RB/CXCR2) increases the permeability of blood
            vessels and increasing levels of IL-8 are positively correlated
            with increased severity of multiple disease outcomes (eg, sepsis).
            This gene and other members of the CXC chemokine gene family form a
            gene cluster in a region of chromosome 4q. [provided by RefSeq, May
            2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK131067.1, SRR5189667.172257.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in cytokine storm inflammatory
                                      response
            MANE Ensembl match     :: ENST00000307407.8/ ENSP00000306512.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                BG497712.1         65-114
            51-1642             BC013615.1         1-1592
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q13.3"
     gene            1..1642
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /note="C-X-C motif chemokine ligand 8"
                     /db_xref="GeneID:3576"
                     /db_xref="HGNC:HGNC:6025"
                     /db_xref="MIM:146930"
     exon            1..154
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..390
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; tumor necrosis factor-induced gene 1; alveolar
                     macrophage chemotactic factor I; lymphocyte derived
                     neutrophil activating peptide; lung giant cell
                     carcinoma-derived chemotactic protein; beta endothelial
                     cell-derived neutrophil activating peptide; granulocyte
                     chemotactic protein 1; beta-thromboglobulin-like protein;
                     emoctakin; monocyte-derived neutrophil chemotactic factor;
                     chemokine (C-X-C motif) ligand 8; neutrophil-activating
                     peptide 1; small inducible cytokine subfamily B, member 8;
                     interleukin 8; T-cell chemotactic factor; monocyte-derived
                     neutrophil-activating peptide"
                     /codon_start=1
                     /product="interleukin-8 isoform 1 precursor"
                     /protein_id="NP_000575.1"
                     /db_xref="CCDS:CCDS34005.1"
                     /db_xref="GeneID:3576"
                     /db_xref="HGNC:HGNC:6025"
                     /db_xref="MIM:146930"
                     /translation="MTSKLAVALLAAFLISAALCEGAVLPRSAKELRCQCIKTYSKPF
                     HPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS"
     exon            155..290
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /inference="alignment:Splign:2.1.0"
     exon            291..374
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /inference="alignment:Splign:2.1.0"
     exon            375..1642
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1620..1625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /note="hexamer: AATATA"
     polyA_site      1642
                     /gene="CXCL8"
                     /gene_synonym="GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF;
                     MONAP; NAF; NAP-1; NAP1; SCYB8"
                     /note="major polyA site"
ORIGIN      
        1 acaaactttc agagacagca gagcacacaa gcttctagga caagagccag gaagaaacca
       61 ccggaaggaa ccatctcact gtgtgtaaac atgacttcca agctggccgt ggctctcttg
      121 gcagccttcc tgatttctgc agctctgtgt gaaggtgcag ttttgccaag gagtgctaaa
      181 gaacttagat gtcagtgcat aaagacatac tccaaacctt tccaccccaa atttatcaaa
      241 gaactgagag tgattgagag tggaccacac tgcgccaaca cagaaattat tgtaaagctt
      301 tctgatggaa gagagctctg tctggacccc aaggaaaact gggtgcagag ggttgtggag
      361 aagtttttga agagggctga gaattcataa aaaaattcat tctctgtggt atccaagaat
      421 cagtgaagat gccagtgaaa cttcaagcaa atctacttca acacttcatg tattgtgtgg
      481 gtctgttgta gggttgccag atgcaataca agattcctgg ttaaatttga atttcagtaa
      541 acaatgaata gtttttcatt gtaccatgaa atatccagaa catacttata tgtaaagtat
      601 tatttatttg aatctacaaa aaacaacaaa taatttttaa atataaggat tttcctagat
      661 attgcacggg agaatataca aatagcaaaa ttgaggccaa gggccaagag aatatccgaa
      721 ctttaatttc aggaattgaa tgggtttgct agaatgtgat atttgaagca tcacataaaa
      781 atgatgggac aataaatttt gccataaagt caaatttagc tggaaatcct ggattttttt
      841 ctgttaaatc tggcaaccct agtctgctag ccaggatcca caagtccttg ttccactgtg
      901 ccttggtttc tcctttattt ctaagtggaa aaagtattag ccaccatctt acctcacagt
      961 gatgttgtga ggacatgtgg aagcacttta agttttttca tcataacata aattattttc
     1021 aagtgtaact tattaaccta tttattattt atgtatttat ttaagcatca aatatttgtg
     1081 caagaatttg gaaaaataga agatgaatca ttgattgaat agttataaag atgttatagt
     1141 aaatttattt tattttagat attaaatgat gttttattag ataaatttca atcagggttt
     1201 ttagattaaa caaacaaaca attgggtacc cagttaaatt ttcatttcag ataaacaaca
     1261 aataattttt tagtataagt acattattgt ttatctgaaa ttttaattga actaacaatc
     1321 ctagtttgat actcccagtc ttgtcattgc cagctgtgtt ggtagtgctg tgttgaatta
     1381 cggaataatg agttagaact attaaaacag ccaaaactcc acagtcaata ttagtaattt
     1441 cttgctggtt gaaacttgtt tattatgtac aaatagattc ttataatatt atttaaatga
     1501 ctgcattttt aaatacaagg ctttatattt ttaactttaa gatgttttta tgtgctctcc
     1561 aaattttttt tactgtttct gattgtatgg aaatataaaa gtaaatatga aacatttaaa
     1621 atataatttg ttgtcaaagt aa
//
