LOCUS       NM_017748               3067 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens CWC25 spliceosome associated protein homolog (CWC25),
            transcript variant 1, mRNA.
ACCESSION   NM_017748
VERSION     NM_017748.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Zhan,X., Yan,C., Zhang,X., Lei,J. and Shi,Y.
  TITLE     Structure of a human catalytic step I spliceosome
  JOURNAL   Science 359 (6375), 537-545 (2018)
   PUBMED   29301961
REFERENCE   3  (bases 1 to 3067)
  AUTHORS   Fabrizio,P., Dannenberg,J., Dube,P., Kastner,B., Stark,H.,
            Urlaub,H. and Luhrmann,R.
  TITLE     The evolutionarily conserved core design of the catalytic
            activation step of the yeast spliceosome
  JOURNAL   Mol Cell 36 (4), 593-608 (2009)
   PUBMED   19941820
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC008833.2, AC006449.19 and
            AW612704.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017748.4.
            
            Summary: This gene encodes a factor that is part of the
            multi-protein C complex involved in pre-mRNA splicing.
            Alternatively spliced transcripts have been described for this
            gene. [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the supported protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.233348.1,
                                           SRR1660809.244455.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000614790.5/ ENSP00000478070.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2315              BC008833.2         16-2330
            2316-3058           AC006449.19        245453-246195       c
            3059-3067           AW612704.1         17-25               c
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..3067
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /note="CWC25 spliceosome associated protein homolog"
                     /db_xref="GeneID:54883"
                     /db_xref="HGNC:HGNC:25989"
     exon            1..156
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1416
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /note="coiled-coil domain-containing protein 49"
                     /codon_start=1
                     /product="pre-mRNA-splicing factor CWC25 homolog"
                     /protein_id="NP_060218.1"
                     /db_xref="CCDS:CCDS45663.1"
                     /db_xref="GeneID:54883"
                     /db_xref="HGNC:HGNC:25989"
                     /translation="MGGGDLNLKKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQREL
                     REERAREEMQRYAEDVGAVKKKEEKLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEE
                     KEAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKM
                     KKIKELLQMSLEKKEKKKKKEKKKKHKKHKHRSSSSDRSSSEDEHSAGRSQKKMANSS
                     PVLSKVPGYGLQVRNSDRNQGLQGPLTAEQKRGHGMKNHSRSRSSSHSPPRHASKKST
                     REAGSRDRRSRSLGRRSRSPRPSKLHNSKVNRRETGQTRSPSPKKEVYQRRHAPGYTR
                     KLSAEELERKRQEMMENAKWREEERLNILKRHAKDEEREQRLEKLDSRDGKFIHRMKL
                     ESASTSSLEDRVKRNIYSLQRTSVALEKNFMKR"
     exon            157..329
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            330..566
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            567..636
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            637..764
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            765..828
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            829..1040
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1139
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1301
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..3067
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2037
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /note="major polyA site"
     regulatory      3033..3038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /note="hexamer: AATAAA"
     polyA_site      3067
                     /gene="CWC25"
                     /gene_synonym="CCDC49"
                     /note="major polyA site"
ORIGIN      
        1 agagcggtgg tgaaactggc agttgacggc tcctgggact agatcccgcg aggtagcccc
       61 cgaactattt ctctacgttt tctcttgatc ctcccgaaat cttccagatc cgcgtagtga
      121 ggaatcgtct ccaccgtcat ggggggcgga gacctgaatc tgaagaagag ctggcacccg
      181 cagaccctca ggaatgtgga gaaagtgtgg aaggccgagc agaagcatga ggctgagcgg
      241 aagaagattg aggagcttca gcgggagctg cgagaagaga gagcccggga agagatgcag
      301 cgctatgcgg aggatgttgg ggccgtcaag aaaaaagaag aaaagttgga ctggatgtac
      361 cagggtcctg gtgggatggt gaaccgtgac gagtacctgc tggggcgccc cattgacaaa
      421 tatgtttttg agaagatgga ggagaaggag gcaggctgct cttctgaaac aggacttctc
      481 ccaggctcta tctttgcccc atcaggtgcc aattcccttc ttgacatggc cagcaagatc
      541 cgggaggacc cactcttcat catcaggaag aaggaggagg agaaaaaacg agaggtatta
      601 aataatccag tgaaaatgaa gaaaatcaaa gaattgttgc aaatgagtct ggaaaaaaag
      661 gagaagaaga aaaagaagga gaagaaaaag aagcacaaga aacataagca cagaagctcg
      721 agtagtgatc gttccagcag cgaggatgag cacagtgcag ggagatcaca gaagaagatg
      781 gcaaattcct cccctgtttt gtccaaagtc cctggatatg gcttacaggt ccggaactct
      841 gaccgtaacc agggtcttca gggtcctctg acagcagagc aaaagagagg gcatgggatg
      901 aagaaccatt ccagatccag aagctcctcc cactcacccc caagacatgc cagcaagaag
      961 agcaccaggg aagcagggtc ccgggacagg aggtctcgat ccctgggcag aaggtcacgg
     1021 tccccaagac ccagcaaact gcacaactct aaggtgaaca ggagagagac aggccaaact
     1081 aggagcccat cacctaaaaa agaggtctac caaaggcgac atgctcccgg atacaccaga
     1141 aaactctctg cagaggaatt agagcgaaaa cggcaagaga tgatggaaaa cgccaaatgg
     1201 agggaggagg agagactgaa catcctcaag aggcatgcta aggatgagga acgggagcag
     1261 aggctagaga agctggactc ccgggatggg aagttcatcc accgcatgaa gctggagagt
     1321 gcatctactt cctccctgga ggatcgggtg aagcggaata tctactcttt acagagaact
     1381 tcggtagctc tggagaagaa ctttatgaaa agatgaaaac tgtcccctct cttattggtt
     1441 ttcctgcatt ttccagggaa gctgctgacc ccttaattct ctttataaga gttcaaatga
     1501 cttctttcac agatgtcaaa ccaccagtgt tcaaagtgac cctgcttcat tgagtcctga
     1561 aacagctcac ttcctttgag agctagtgtg acttgctttg tgggacactc agtaactttg
     1621 ggttttgact ctttaacggg tgggcactgg accatctcgg tgggagtgct tgtgccactc
     1681 tggaaggctg ttccctgggg ttgtgatgtt tatcatgcca cttccttctt acctgtgcca
     1741 acagacctat ttcactgcct cagcgtacac cagacccttc agaaacctct ctggtgtcac
     1801 ccagatagat tgtgcttact gagacaaatg aacgtttact tgatttagaa gataatgtga
     1861 cagaatgatg tcaggttagg tcaaagccaa gggagtgaca gaatctggaa aatcaaacaa
     1921 tacaaaaagc cctaaatgaa ctgttaacta tttgatcttt ggatgtaaaa ttgtaatgcg
     1981 tatatgtaca aatgtacaat ttttacatgc ttttaaaaaa ggttagcttt gtgaaaatac
     2041 cttgtttggt caatgacttt actgggtaat agaaccacat tgaaccttga tggcaagtaa
     2101 tacaataagg caggccagct cgtttttctc tctgaatctg gctggtttag gaggagcctg
     2161 ggtttatcga cgagatctgg agtatctatt cttttccact gcttgcagtc tccaatgtag
     2221 gcagtgtaaa ggtatagtaa aatgatttta ggagtcagaa ccaaattgcc aatatgctcc
     2281 atggctccta aaggaaaata aaatggaagt ttttattttg cggcttcact tcttaccgtg
     2341 tagtaacctt actacaatta cagattttca ggagttgttt gctagtctct ttcagtcagg
     2401 tactaaaaaa aaaacaaaaa acaggctggg cgcggtggct cacacctgta atctcagcac
     2461 tttgggaggc tgaggtgggc ggatcacgag gtcaggagat cgagaccatc ctggcgaaca
     2521 cggtgaaacc ccgtctctac taaaaaaata caaaaaatta gccgggcgtg gtggcgggcg
     2581 cctgtagtcc cagctactcg ggaggctgag gcaggagaat ggcgtgaacc caggaggcgg
     2641 agcttgcagt gagccgagat tgtgccaccg cactccagcc tgggtgacag agcgagattc
     2701 cgtctcaaaa aacaacagca acaaaacaaa accctcgagg ggccactctc ctgtctttac
     2761 tccttttccc ttctctattc tttcaccaga agccctcatt tgaccagtga actcctaggc
     2821 cctcttgacc cgcacattag ctgggcgatt tccttgttct gctaattcct aattctgctt
     2881 aaaatgtatt tggatttctg tttttgaaca cttatgatgc caggcactgt aatgcttgaa
     2941 acccgatctt tccctagaga atgtaacata cgtttttatt catttaatca cttcattatg
     3001 ccggggttaa ttatgtttat tttataattg gtaataaagg ccacatttat ttttgtaact
     3061 gtttaaa
//
