LOCUS       NM_020943               3524 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens CWC22 spliceosome associated protein homolog (CWC22),
            transcript variant 1, mRNA.
ACCESSION   NM_020943 XM_034594
VERSION     NM_020943.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Busetto,V., Barbosa,I., Basquin,J., Marquenet,E., Hocq,R.,
            Hennion,M., Paternina,J.A., Namane,A., Conti,E., Bensaude,O. and Le
            Hir,H.
  TITLE     Structural and functional insights into CWC27/CWC22 heterodimer
            linking the exon junction complex to spliceosomes
  JOURNAL   Nucleic Acids Res 48 (10), 5670-5683 (2020)
   PUBMED   32329775
  REMARK    GeneRIF: Structural and functional insights into CWC27/CWC22
            heterodimer linking the exon junction complex to spliceosomes.
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Han,S.W., Ahn,J.Y., Lee,S., Noh,Y.S., Jung,H.C., Lee,M.H.,
            Park,H.J., Chun,H.J., Choi,S.J., Kim,E.S. and Lee,J.Y.
  TITLE     Gene expression network analysis of lymph node involvement in colon
            cancer identifies AHSA2, CDK10, and CWC22 as possible prognostic
            markers
  JOURNAL   Sci Rep 10 (1), 7170 (2020)
   PUBMED   32345988
  REMARK    GeneRIF: Gene expression network analysis of lymph node involvement
            in colon cancer identifies AHSA2, CDK10, and CWC22 as possible
            prognostic markers.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3524)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3524)
  AUTHORS   Trepte,P., Kruse,S., Kostova,S., Hoffmann,S., Buntru,A.,
            Tempelmeier,A., Secker,C., Diez,L., Schulz,A., Klockmeier,K.,
            Zenkner,M., Golusik,S., Rau,K., Schnoegl,S., Garner,C.C. and
            Wanker,E.E.
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology
            for quantitative mapping of protein-protein interactions in
            mammalian cells
  JOURNAL   Mol Syst Biol 14 (7), e8071 (2018)
   PUBMED   29997244
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3524)
  AUTHORS   Zhang,X., Yan,C., Zhan,X., Li,L., Lei,J. and Shi,Y.
  TITLE     Structure of the human activated spliceosome in three
            conformational states
  JOURNAL   Cell Res 28 (3), 307-322 (2018)
   PUBMED   29360106
REFERENCE   6  (bases 1 to 3524)
  AUTHORS   Tao,S., Wang,Z., Feng,J., Hsu,F.C., Jin,G., Kim,S.T., Zhang,Z.,
            Gronberg,H., Zheng,L.S., Isaacs,W.B., Xu,J. and Sun,J.
  TITLE     A genome-wide search for loci interacting with known prostate
            cancer risk-associated genetic variants
  JOURNAL   Carcinogenesis 33 (3), 598-603 (2012)
   PUBMED   22219177
REFERENCE   7  (bases 1 to 3524)
  AUTHORS   Hegele,A., Kamburov,A., Grossmann,A., Sourlis,C., Wowro,S.,
            Weimann,M., Will,C.L., Pena,V., Luhrmann,R. and Stelzl,U.
  TITLE     Dynamic protein-protein interaction wiring of the human spliceosome
  JOURNAL   Mol Cell 45 (4), 567-580 (2012)
   PUBMED   22365833
REFERENCE   8  (bases 1 to 3524)
  AUTHORS   Lehner,B. and Sanderson,C.M.
  TITLE     A protein interaction framework for human mRNA degradation
  JOURNAL   Genome Res 14 (7), 1315-1323 (2004)
   PUBMED   15231747
REFERENCE   9  (bases 1 to 3524)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   10 (bases 1 to 3524)
  AUTHORS   Kelly,W.G., Xu,S., Montgomery,M.K. and Fire,A.
  TITLE     Distinct requirements for somatic and germline expression of a
            generally expressed Caernorhabditis elegans gene
  JOURNAL   Genetics 146 (1), 227-238 (1997)
   PUBMED   9136012
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC068194.6, BC031216.1, AB046824.1 and AC096587.1.
            
            On Nov 24, 2018 this sequence version replaced NM_020943.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031216.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000410053.8/ ENSP00000387006.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-244               AC068194.6         7523-7766           c
            245-1359            BC031216.1         1-1115
            1360-3523           AB046824.1         935-3098
            3524-3524           AC096587.1         36202-36202         c
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.3"
     gene            1..3524
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /note="CWC22 spliceosome associated protein homolog"
                     /db_xref="GeneID:57703"
                     /db_xref="HGNC:HGNC:29322"
                     /db_xref="MIM:615186"
     exon            1..431
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            432..571
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     CDS             545..3271
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /note="isoform a is encoded by transcript variant 1;
                     functional spliceosome-associated protein b;
                     pre-mRNA-splicing factor CWC22 homolog; nucampholin
                     homolog; CWC22 homolog, spliceosome-associated protein;
                     CWC22 spliceosome-associated protein"
                     /codon_start=1
                     /product="pre-mRNA-splicing factor CWC22 homolog isoform
                     a"
                     /protein_id="NP_065994.1"
                     /db_xref="CCDS:CCDS46465.1"
                     /db_xref="GeneID:57703"
                     /db_xref="HGNC:HGNC:29322"
                     /db_xref="MIM:615186"
                     /translation="MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRD
                     YFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETS
                     VTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWE
                     ALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAAL
                     VAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLE
                     MLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQY
                     MIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFM
                     ENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEI
                     NLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFG
                     LLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLE
                     CIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNT
                     RFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDS
                     SDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTN
                     DRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYS
                     RVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKE
                     MNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK"
     exon            572..639
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            640..750
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            751..996
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1125
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1294
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1348
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1484
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1691
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1754
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1859
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1941
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            1942..2002
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2080
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2233
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2361
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            2362..2463
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2684
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     exon            2685..3524
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3504..3509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /note="hexamer: AATAAA"
     polyA_site      3524
                     /gene="CWC22"
                     /gene_synonym="EIF4GL; fSAPb; NCM"
                     /note="major polyA site"
ORIGIN      
        1 aacttctctt ttttgtcgcc attttggcaa caagacagtg tgcctggatc gactactttg
       61 actctggtat tatgatatgg tatttgaaat aaattaaagg gataacgtgt caagccaatt
      121 gagcgtcaag tttttaaata tttcactatt cgccttcttc ctaccgtcgg tgctcgggaa
      181 gaggaagtga cgtacgtccg gtgtaatggc ggcgcggtgg agctctaggt aattatcgaa
      241 ctccgtggag tccccgcagg cagagacgcg gagtgggggt ggcgggtcgc cggatcgggt
      301 gggaagtaaa agacccccag gagtgggtgc gggctcactg atacggctgt gagcggcatt
      361 ggagtgtgcg tgggtcttct cgctacgctc gggcccgcaa gaggggtgtg agagcggcta
      421 ggtccaagca ggtgatgttt gcagacagaa aaagtaaact gtcaaggaat tcagagcatt
      481 gggtacttgt tatttggacc agtttgaagc atctttgatt tattggacca actggcaaca
      541 gaaaatgaaa agtagtgtgg cacagataaa accttcttct ggtcatgaca gaagggaaaa
      601 ccttaattca tatcagagga actcctctcc agaagacaga tatgaagaac aagaacgatc
      661 cccccgggat agagattact ttgattacag cagatcagac tatgagcatt caagaagagg
      721 acgttcttat gatagtagca tggagtcacg aaacagggac cgagaaaaac gcagagaaag
      781 agaaagagat acggatcgga aaaggtctcg gaaatcccca tctcctggga ggagaaaccc
      841 agaaacatca gtaactcaga gttcctctgc tcaggatgaa cctgctacaa agaaaaagaa
      901 agatgagctg gatcctcttc ttactcgcac tggtggagca tatattcccc ctgcaaagct
      961 caggatgatg caggaacaga ttacagataa aaacagctta gcataccaga ggatgagttg
     1021 ggaggccctg aagaagtcaa ttaatggcct tatcaacaaa gtcaacattt ccaacataag
     1081 tattattatt caagagcttc ttcaagaaaa tatagttaga ggaagaggac tgctgtccag
     1141 gtctgttttg caagcacaga gtgcttctcc aatcttcacc catgtttatg cagcattagt
     1201 ggcaattatc aactcaaaat ttccacaaat tggagaatta atcctcaaaa ggttaattct
     1261 taattttcga aaaggctatc gaagaaatga caagcaactt tgcctgactg cttcaaaatt
     1321 tgtggcgcat cttattaacc aaaatgtggc acacgaagta ttatgcttag agatgctcac
     1381 tttgctcctg gaaagaccaa cagatgatag cgttgaagta gctattggtt ttcttaagga
     1441 atgtggcctc aaattaacac aagtgtcacc aagaggaatc aatgctatat ttgaacgcct
     1501 tcgaaacatt ctgcatgagt ctgaaattga caaaagagtt caatatatga ttgaagtgat
     1561 gtttgctgta cggaaagatg gattcaagga ccaccccatt atcctagaag gtcttgattt
     1621 ggtggaagaa gatgatcaat tcactcatat gctccctctg gaggatgact ataatccaga
     1681 agatgttctt aatgttttca agatggatcc taattttatg gagaatgaag agaagtacaa
     1741 agctattaag aaagaaattc ttgatgaggg agatactgac tcgaacacag accaggatgc
     1801 tgggagtagt gaagaggacg aggaagaaga agaggaagag ggagaagaag atgaagaagg
     1861 acaaaaagta actattcatg acaaaacaga aattaacctg gtctcatttc gtcgtacaat
     1921 ttatcttgct attcagtcaa gtttagattt tgaagaatgt gctcacaaat tgctgaaaat
     1981 ggagtttcct gaaagccaaa caaaagaact ctgcaacatg atacttgatt gctgtgccca
     2041 acagaggaca tacgaaaaat tttttggctt attagctggg cgattttgca tgctaaagaa
     2101 agagtacatg gaatcctttg aaggtatatt caaagaacag tatgatacca tccatcgctt
     2161 ggaaacaaac aagttgcgaa atgttgctaa gatgtttgct caccttttat acactgattc
     2221 acttccatgg agtgttcttg aatgtataaa actgagtgaa gaaaccacta catcatccag
     2281 tagaattttt gtcaaaatat ttttccagga actgtgtgaa tacatgggtc ttcctaaact
     2341 taatgcaaga ttaaaggatg aaactctgca gccattcttt gaaggattat taccccgaga
     2401 taatccaaga aacactcggt ttgccatcaa cttctttact tctataggtc ttggaggttt
     2461 aacggatgaa ctgcgggagc atctcaaaaa tacaccaaag gtcattgtgg cgcagaaacc
     2521 agatgttgag caaaataaat cctccccatc ctcttcctct tcagcgtcct cctcttcaga
     2581 gtctgactca tccgactctg attctgacag cagtgatagc agttcagagt cttccagtga
     2641 agagagcgac tcttcatcca tcagtagtca tagctctgcc tcagctaatg atgtaagaaa
     2701 gaagggacat gggaagacca gaagtaaaga ggtagataaa ttgatcagaa accagcaaac
     2761 aaatgatagg aaacaaaaag aaagaagaca agaacacggg caccaggaaa caaggactga
     2821 gagagaaaga aggtcagaaa aacacagaga tcaaaattca agtggttcaa attggagaga
     2881 tcctataaca aagtacacat cagacaaaga tgttccttct gaacgaaata actacagtag
     2941 agttgcgaat gacagagacc aagaaatgca tatagatttg gaaaataagc atggtgatcc
     3001 caaaaagaag agaggagaga gaagaaattc tttttctgaa aatgagaagc atacacaccg
     3061 aattaaagac agtgaaaatt tcagaagaaa agatagatca aagtcaaagg aaatgaatag
     3121 aaagcactca ggctcaagaa gtgatgaaga tagatatcaa aatggtgccg agagacgatg
     3181 ggaaaaatct agcagatact ctgaacaatc cagagaatca aagaaaaatc aggaccggcg
     3241 aagagaaaag tctccagcaa aacaaaaata attctacaaa atgacataaa ctggacatgc
     3301 cttatattca gttgaaacaa attatttttt actttgacga actttataga gaattcttgt
     3361 ataaagtatt ggtttgtatt tgtaaattta aaaaatgttt tcattttaac atgttttata
     3421 attgatacat ctcaaggccc tacccataaa attatttgaa aactttcacc agaaagggat
     3481 gtaagtctcg cttatatttt gtgaataaaa tgatcaaaag ctca
//
