LOCUS       NM_016403               1522 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens CWC15 spliceosome associated protein homolog (CWC15),
            transcript variant 3, mRNA.
ACCESSION   NM_016403
VERSION     NM_016403.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1522)
  AUTHORS   Bertram,K., Agafonov,D.E., Liu,W.T., Dybkov,O., Will,C.L.,
            Hartmuth,K., Urlaub,H., Kastner,B., Stark,H. and Luhrmann,R.
  TITLE     Cryo-EM structure of a human spliceosome activated for step 2 of
            splicing
  JOURNAL   Nature 542 (7641), 318-323 (2017)
   PUBMED   28076346
REFERENCE   4  (bases 1 to 1522)
  AUTHORS   van Maldegem,F., Maslen,S., Johnson,C.M., Chandra,A., Ganesh,K.,
            Skehel,M. and Rada,C.
  TITLE     CTNNBL1 facilitates the association of CWC15 with CDC5L and is
            required to maintain the abundance of the Prp19 spliceosomal
            complex
  JOURNAL   Nucleic Acids Res 43 (14), 7058-7069 (2015)
   PUBMED   26130721
  REMARK    GeneRIF: The results show that CTNNBL1 enhances the association of
            CWC15 and CDC5L, both core Prp19 complex proteins and identify an
            overlap in the region of CDC5L that binds either CTNNBL1 or CWC15
            suggesting the two proteins might exchange places in the complex.
REFERENCE   5  (bases 1 to 1522)
  AUTHORS   Chanarat,S. and Strasser,K.
  TITLE     Splicing and beyond: the many faces of the Prp19 complex
  JOURNAL   Biochim Biophys Acta 1833 (10), 2126-2134 (2013)
   PUBMED   23742842
  REMARK    Review article
REFERENCE   6  (bases 1 to 1522)
  AUTHORS   Satoh,J., Obayashi,S., Misawa,T., Sumiyoshi,K., Oosumi,K. and
            Tabunoki,H.
  TITLE     Protein microarray analysis identifies human cellular prion protein
            interactors
  JOURNAL   Neuropathol Appl Neurobiol 35 (1), 16-35 (2009)
   PUBMED   18482256
REFERENCE   7  (bases 1 to 1522)
  AUTHORS   Anney,R.J., Lasky-Su,J., O'Dushlaine,C., Kenny,E., Neale,B.M.,
            Mulligan,A., Franke,B., Zhou,K., Chen,W., Christiansen,H.,
            Arias-Vasquez,A., Banaschewski,T., Buitelaar,J., Ebstein,R.,
            Miranda,A., Mulas,F., Oades,R.D., Roeyers,H., Rothenberger,A.,
            Sergeant,J., Sonuga-Barke,E., Steinhausen,H., Asherson,P.,
            Faraone,S.V. and Gill,M.
  TITLE     Conduct disorder and ADHD: evaluation of conduct problems as a
            categorical and quantitative trait in the international multicentre
            ADHD genetics study
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 147B (8), 1369-1378 (2008)
   PUBMED   18951430
REFERENCE   8  (bases 1 to 1522)
  AUTHORS   Jurica,M.S., Licklider,L.J., Gygi,S.R., Grigorieff,N. and
            Moore,M.J.
  TITLE     Purification and characterization of native spliceosomes suitable
            for three-dimensional structural analysis
  JOURNAL   RNA 8 (4), 426-439 (2002)
   PUBMED   11991638
REFERENCE   9  (bases 1 to 1522)
  AUTHORS   Ohi,M.D., Link,A.J., Ren,L., Jennings,J.L., McDonald,W.H. and
            Gould,K.L.
  TITLE     Proteomics analysis reveals stable multiprotein complexes in both
            fission and budding yeasts containing Myb-related Cdc5p/Cef1p,
            novel pre-mRNA splicing factors, and snRNAs
  JOURNAL   Mol Cell Biol 22 (7), 2011-2024 (2002)
   PUBMED   11884590
REFERENCE   10 (bases 1 to 1522)
  AUTHORS   O'Brien,K.P., Tapia-Paez,I., Stahle-Backdahl,M., Kedra,D. and
            Dumanski,J.P.
  TITLE     Characterization of five novel human genes in the 11q13-q22 region
  JOURNAL   Biochem Biophys Res Commun 273 (1), 90-94 (2000)
   PUBMED   10873569
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC040946.1, BM971024.1, CA413488.1 and AP002383.3.
            
            On Nov 22, 2018 this sequence version replaced NM_016403.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.162484.1,
                                           SRR3476690.988637.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146, SAMN03267760
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000279839.8/ ENSP00000475615.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-803               BC040946.1         9-811
            804-1027            BM971024.1         16-239              c
            1028-1520           CA413488.1         17-509              c
            1521-1522           AP002383.3         85592-85593         c
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q21"
     gene            1..1522
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /note="CWC15 spliceosome associated protein homolog"
                     /db_xref="GeneID:51503"
                     /db_xref="HGNC:HGNC:26939"
     exon            1..59
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            60..198
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..757
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /note="CWC15 spliceosome-associated protein"
                     /codon_start=1
                     /product="spliceosome-associated protein CWC15 homolog"
                     /protein_id="NP_057487.2"
                     /db_xref="CCDS:CCDS73369.1"
                     /db_xref="GeneID:51503"
                     /db_xref="HGNC:HGNC:26939"
                     /translation="MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYR
                     QTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAAN
                     LDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRM
                     ENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSE
                     FHKKFMEKYIK"
     exon            199..311
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            312..400
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            401..508
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            509..627
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /inference="alignment:Splign:2.1.0"
     exon            628..1522
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      776..781
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /note="hexamer: ATTAAA"
     polyA_site      797
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /note="major polyA site"
     regulatory      999..1004
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /note="hexamer: AATAAA"
     polyA_site      1027
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
     regulatory      1501..1506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
                     /note="hexamer: AATAAA"
     polyA_site      1522
                     /gene="CWC15"
                     /gene_synonym="AD002; C11orf5; Cwf15; HSPC148; ORF5"
ORIGIN      
        1 aagagtggta atggcgtctg tatgatcttc ggagcctgct gcatcggacc tcggccagtc
       61 ataaaagatg acaacagcag ccaggccaac ctttgaacct gccagaggtg gaaggggaaa
      121 aggagaaggt gatttgagcc aactttcaaa gcagtattca agcagagacc taccctctca
      181 tacaaagata aaatacagac agactactca ggatgcccct gaagaggttc gtaaccgtga
      241 cttcaggaga gagttggaag aaagagagag agctgctgca agagagaaaa atagggatcg
      301 tccaacccga gaacatacaa cctcctcttc agtgtcaaaa aagccacggt tagaccagat
      361 tcctgccgcc aaccttgatg cagatgaccc tctaacagat gaggaagatg aagattttga
      421 agaagaaagt gatgatgatg atactgcagc tcttcttgca gaactggaaa aaattaaaaa
      481 agaaagagct gaagagcagg ccaggaagga acaagaacaa aaagctgaag aagagaggat
      541 tcgtatggaa aacattctga gcggaaaccc tctccttaat ctcactggcc catcccagcc
      601 tcaggccaac ttcaaagtta aaagaaggtg ggatgatgac gttgtcttca agaactgtgc
      661 aaaaggtgta gatgaccaga agaaagacaa aagatttgta aatgacacac tgcgatctga
      721 atttcacaaa aagttcatgg agaaatatat taaatagtac agttttatgt gcttaattaa
      781 agactgtaaa acgtaaagga tcattctgac tttttttatg agtttttttt ttcttttccc
      841 ctgtctaaat tgtgtgtggg cttccccagt tttaaactcc tatctttttc tttagtgata
      901 ccttacttga taatggactt gggaataggt ttaacccagt ttgcattctc caaatgtttc
      961 cagtgatatg ttttcctggg aaaagcaaat ctgcctgtaa taaatatttt taatctaaat
     1021 aaccaaatct gggtgcccaa ctttttgttg gaatttatgc tgctagtaat acattccata
     1081 atatcttttg aaaagaaaaa ttaccattgt gtattgtgct tgtgcattaa cttgtccttt
     1141 tccccctgtt tctctcacat ttaagatgag ttaattcata ttttctctgc attttttaaa
     1201 agagtgtaat ggaagatgag agggattcta ttttggacca catgttggtg tggaggagtg
     1261 tcattgacag taagcacccc aggcgtgtgt ctgggagagc attgggtatc gctcacttct
     1321 gcaggtactt gttttttttt ctcatggccg aagaggagag ctctgtaagc attcttttaa
     1381 agtatacggt actcacttgt gttctggata ctcaactgcc atgtttcctt tgaaagagat
     1441 tgaatatgca cctgacatgc atttagtgca aagcctgaga aagcttcaaa caatatataa
     1501 aataaaacct ttggaaataa ca
//
