LOCUS       NM_001008895            5821 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens cullin 4A (CUL4A), transcript variant 1, mRNA.
ACCESSION   NM_001008895
VERSION     NM_001008895.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Jia,Y., Huo,X., Wu,L., Zhang,H., Xu,W. and Leng,H.
  TITLE     circSNTB2 and CUL4A Induces Dysfunction of Nucleus Pulposus Cells
            by Competitively Binding miR-665
  JOURNAL   Biochem Genet 62 (2), 968-986 (2024)
   PUBMED   37507642
  REMARK    GeneRIF: circSNTB2 and CUL4A Induces Dysfunction of Nucleus
            Pulposus Cells by Competitively Binding miR-665.
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Tavlas,P., Nikou,S., Geramoutsou,C., Bosgana,P., Tsaniras,S.C.,
            Melachrinou,M., Maroulis,I. and Bravou,V.
  TITLE     CUL4A Ubiquitin Ligase Is an Independent Predictor of Overall
            Survival in Pancreatic Adenocarcinoma
  JOURNAL   Cancer Genomics Proteomics 21 (2), 166-177 (2024)
   PUBMED   38423594
  REMARK    GeneRIF: CUL4A Ubiquitin Ligase Is an Independent Predictor of
            Overall Survival in Pancreatic Adenocarcinoma.
REFERENCE   3  (bases 1 to 5821)
  AUTHORS   Hu,Y., Zhang,R., Lu,S., Zhang,W., Wang,D., Ge,Y., Jiang,F., Qin,X.
            and Liu,Y.
  TITLE     S100 Calcium Binding Protein A16 Promotes Cell Proliferation by
            triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to
            inhibit Hippo pathway in Glioma development
  JOURNAL   Int J Biol Sci 19 (7), 2034-2052 (2023)
   PUBMED   37151881
  REMARK    GeneRIF: S100 Calcium Binding Protein A16 Promotes Cell
            Proliferation by triggering LATS1 ubiquitin degradation mediated by
            CUL4A ligase to inhibit Hippo pathway in Glioma development.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5821)
  AUTHORS   Li,X., Han,Y.R., Xuefeng,X., Ma,Y.X., Xing,G.S., Yang,Z.W.,
            Zhang,Z., Shi,L. and Wu,X.L.
  TITLE     Lentivirus-mediated short hairpin RNA interference of CENPK
            inhibits growth of colorectal cancer cells with overexpression of
            Cullin 4A
  JOURNAL   World J Gastroenterol 28 (37), 5420-5443 (2022)
   PUBMED   36312839
  REMARK    GeneRIF: Lentivirus-mediated short hairpin RNA interference of
            CENPK inhibits growth of colorectal cancer cells with
            overexpression of Cullin 4A.
REFERENCE   5  (bases 1 to 5821)
  AUTHORS   Ito,T., Ando,H., Suzuki,T., Ogura,T., Hotta,K., Imamura,Y.,
            Yamaguchi,Y. and Handa,H.
  TITLE     Identification of a primary target of thalidomide teratogenicity
  JOURNAL   Science 327 (5971), 1345-1350 (2010)
   PUBMED   20223979
REFERENCE   6  (bases 1 to 5821)
  AUTHORS   Angers,S., Li,T., Yi,X., MacCoss,M.J., Moon,R.T. and Zheng,N.
  TITLE     Molecular architecture and assembly of the DDB1-CUL4A ubiquitin
            ligase machinery
  JOURNAL   Nature 443 (7111), 590-593 (2006)
   PUBMED   16964240
  REMARK    GeneRIF: crystallographic analyses of the virally hijacked form of
            the human DDB1-CUL4A-ROC1 machinery
REFERENCE   7  (bases 1 to 5821)
  AUTHORS   Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y.
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners
            with an associated ubiquitin ligase activity
  JOURNAL   Mol Cell 3 (4), 535-541 (1999)
   PUBMED   10230407
REFERENCE   8  (bases 1 to 5821)
  AUTHORS   Chen,L.C., Manjeshwar,S., Lu,Y., Moore,D., Ljung,B.M., Kuo,W.L.,
            Dairkee,S.H., Wernick,M., Collins,C. and Smith,H.S.
  TITLE     The human homologue for the Caenorhabditis elegans cul-4 gene is
            amplified and overexpressed in primary breast cancers
  JOURNAL   Cancer Res 58 (16), 3677-3683 (1998)
   PUBMED   9721878
REFERENCE   9  (bases 1 to 5821)
  AUTHORS   Osaka,F., Kawasaki,H., Aida,N., Saeki,M., Chiba,T., Kawashima,S.,
            Tanaka,K. and Kato,S.
  TITLE     A new NEDD8-ligating system for cullin-4A
  JOURNAL   Genes Dev 12 (15), 2263-2268 (1998)
   PUBMED   9694792
REFERENCE   10 (bases 1 to 5821)
  AUTHORS   Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M.
  TITLE     cul-1 is required for cell cycle exit in C. elegans and identifies
            a novel gene family
  JOURNAL   Cell 85 (6), 829-839 (1996)
   PUBMED   8681378
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB455959.1, BC008308.1,
            AL136221.38 and BU631279.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001008895.3.
            
            Summary: CUL4A is the ubiquitin ligase component of a multimeric
            complex involved in the degradation of DNA damage-response proteins
            (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.25465.1,
                                           SRR1660807.245263.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375440.9/ ENSP00000364589.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 DB455959.1         109-112
            5-3256              BC008308.1         1-3252
            3257-5273           AL136221.38        56354-58370
            5274-5821           BU631279.1         15-562              c
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q34"
     gene            1..5821
                     /gene="CUL4A"
                     /note="cullin 4A"
                     /db_xref="GeneID:8451"
                     /db_xref="HGNC:HGNC:2554"
                     /db_xref="MIM:603137"
     exon            1..163
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..2295
                     /gene="CUL4A"
                     /note="isoform 1 is encoded by transcript variant 1;
                     CUL-4A"
                     /codon_start=1
                     /product="cullin-4A isoform 1"
                     /protein_id="NP_001008895.1"
                     /db_xref="CCDS:CCDS41908.1"
                     /db_xref="GeneID:8451"
                     /db_xref="HGNC:HGNC:2554"
                     /db_xref="MIM:603137"
                     /translation="MADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLV
                     IKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPML
                     YKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTY
                     VLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLL
                     GMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVIT
                     YLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQ
                     ALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKE
                     SFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFE
                     AFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHF
                     KQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRK
                     LQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSEL
                     RRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQV
                     STTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLI
                     DRDYMERDKDNPNQYHYVA"
     exon            164..279
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            280..383
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            384..453
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            454..527
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            528..690
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            691..780
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            781..863
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            864..931
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1050
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1243
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1348
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1459
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1545
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1653
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1767
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1873
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2046
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2199
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..5821
                     /gene="CUL4A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3236..3241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL4A"
                     /note="hexamer: ATTAAA"
     polyA_site      3256
                     /gene="CUL4A"
                     /note="major polyA site"
     regulatory      3731..3736
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL4A"
                     /note="hexamer: AATAAA"
     polyA_site      3758
                     /gene="CUL4A"
                     /note="major polyA site"
     regulatory      3766..3771
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL4A"
                     /note="hexamer: AATAAA"
     polyA_site      3788
                     /gene="CUL4A"
     regulatory      5800..5805
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL4A"
                     /note="hexamer: AATATA"
     polyA_site      5821
                     /gene="CUL4A"
ORIGIN      
        1 ggttccggcc cagccatggc ggacgaggcc ccgcggaagg gcagcttctc ggcgctcgtg
       61 ggccgcacca acggcctcac caagcccgcg gccctggccg ccgcgcccgc caagccgggg
      121 ggcgcgggcg gctccaagaa gctggtcatc aagaacttcc gagacagacc tcggctgccc
      181 gacaactaca cgcaggacac gtggcggaag ctgcacgagg cggtgcgggc cgtgcagagc
      241 agcacctcca tcaggtacaa cctcgaggag ctctaccagg ctgtggaaaa tctctgttct
      301 cacaaagtct ccccaatgct ctacaagcaa ctgcgtcagg cctgtgaaga ccacgtccag
      361 gcacagatcc ttccgtttag agaagactca ctagatagtg ttttattttt aaagaagatt
      421 aacacgtgct ggcaggacca ctgcagacaa atgatcatga tcagaagcat cttcctgttc
      481 ttggaccgca cctatgtgct gcagaactcc acgctgccct ccatctggga tatgggatta
      541 gaactgttta gaacccatat tattagtgat aaaatggttc agagtaaaac cattgatgga
      601 atcctactgc tgatcgagcg cgagaggagc ggcgaggccg tggaccggag cctgttgcgg
      661 agcctcctgg gcatgctgtc tgacctgcag gtgtataaag attcatttga actgaaattt
      721 ttggaagaga ctaattgctt atatgctgcc gaaggccaaa ggttaatgca ggaaagagag
      781 gttccagaat atcttaacca tgtaagtaaa cgcttagagg aagagggaga cagagtaatc
      841 acttacttgg accacagcac acagaaacca ctgattgctt gtgtggagaa acagctatta
      901 ggagaacatt taacagcaat tctgcagaaa gggctcgacc acttactgga tgagaacaga
      961 gtgccggacc tcgcacagat gtaccagctg ttcagccggg tgaggggcgg gcagcaggcg
     1021 ctgctgcagc actggagcga gtacatcaag acttttggaa cagcgatcgt aatcaatcct
     1081 gagaaagaca aagacatggt ccaagacctg ttggacttca aggacaaggt ggaccacgtg
     1141 atcgaggtct gcttccagaa gaatgagcgg ttcgtcaacc tgatgaagga gtcctttgag
     1201 acgttcatca acaagagacc caacaagcct gcagaactga tcgcaaagca tgtggattca
     1261 aagttaagag caggcaacaa agaagccaca gacgaggagc tggagcggac gttggacaag
     1321 atcatgatcc tgttcaggtt tatccacggt aaagatgtct ttgaagcatt ttataaaaaa
     1381 gatttggcaa aaagactcct tgttgggaaa agtgcctcag tcgatgctga aaagtctatg
     1441 ttgtcaaagc tcaagcatga gtgcggtgca gccttcacca gcaagctgga aggcatgttc
     1501 aaggacatgg agctttcgaa ggacatcatg gttcatttca agcagcatat gcagaatcag
     1561 agtgactcag gccctataga cctcacagtg aacatactca caatgggcta ctggccaaca
     1621 tacacgccca tggaagtgca cttaacccca gaaatgatta aacttcagga agtatttaag
     1681 gcattttatc ttggaaagca cagtggtcga aaacttcagt ggcaaactac tttgggacat
     1741 gctgttttaa aagcggagtt taaagaaggg aagaaggaat tccaggtgtc cctcttccag
     1801 acactggtgc tcctcatgtt caacgaggga gatggcttca gctttgagga gataaaaatg
     1861 gccacgggga tagaggatag tgaattgcgc agaacgctgc agtccctggc ctgtggcaaa
     1921 gcacgtgtgc tgattaaaag tcccaaagga aaggaagtgg aagatggaga caagttcatt
     1981 tttaatggag agttcaagca caagttgttt agaataaaga tcaatcaaat tcagatgaag
     2041 gaaactgttg aggaacaggt tagcaccact gagagagtgt ttcaggatag acaatatcag
     2101 attgatgctg ctatcgtcag aataatgaag atgagaaaga ctcttggtca taatcttcta
     2161 gtttctgaat tatataatca gctgaaattt ccagtaaagc ctggagattt gaaaaagaga
     2221 attgaatctc tgatagacag agactatatg gagagagaca aagacaatcc gaatcagtac
     2281 cactacgtgg cctgacgcat ctgcagacgg ttccccttca tgaaacacta gaatgtaccc
     2341 tcagagcagg aagcacacct gtgccatttc tgggactctg attgatccag ctgtggacat
     2401 tggaaggcga aggaagggag gtggctcctg ggtcatcttt cacaaggctc aagacttcaa
     2461 cctgcagatg tatctttttc cctccagttt ttcctctagt tcttttaggc atttaaattg
     2521 tttctgttac tctgtgcaaa ataactttga gattggacaa gaagatgtta ctaaagagaa
     2581 gttcctttaa aaggtcttgt tcttgtgtca aaaagctgca agtttggttt gttctcgtgt
     2641 gtgatcatga gtgcacaatg aagaagaccc tagatgctgc attttttagc tctgaagatt
     2701 ccttaggtat ccctgaagac agctcgctca gatgatcagc atttagagtg aaaacaaggg
     2761 cccttcatgg gtgaacatta gaaagagcca gggttcaaag ctggcgaatg gatgacgcac
     2821 cctagccact ggcccctctc tgtttcatgt atttccaaaa gttgtaaact ttgatggctg
     2881 atttttcgta agtcaggttt ctaagtgagc tccctgaggt gccaaggcca tggtgtccgc
     2941 cctgctgcgt ctgttcgtca gctgagttcc ttgtgaatct ctgttttagg gtttggggct
     3001 agtgtgtttg tgtttccatt ctaagattga gtctggcagt ccctgttttt ttgcattggg
     3061 gtaactgctc tttgattttt tttaattgca gtatttgtgt gattgcaata ataaagtttg
     3121 gtttggtttt tacagtcatg cgcagggacg atccttgttc tctgctgtaa actgtaaaaa
     3181 gtttatggag acttaaagtc ttgatgttgt gaagcagagg ttattttgtg gaaagattaa
     3241 aaggattttg ttggtacctg gttttgtgtt gtgtatatat acatgaggtt gaacagtgaa
     3301 aggaaagttc agtagtgatg ttagaagggt aactatgaca aagatacttt tgagataaca
     3361 tttaaaagta ctttatattt tacataatag catgtttcat tttgattaaa agctaccaaa
     3421 ggaattttga tcatggcata agtgtttaaa gcaatatttt ctggaatata ccaagtttat
     3481 ataatttgat tttgtgctaa attattaaga gtctcttttt gaaacatgcg ggtttgaaat
     3541 atgacacctt gtgggtttcc atattaaaat cctcactctt taattgtcat ttctatcttt
     3601 gaaaattttc atttatgagt tccatgatat gtggtctaag aaagaccaaa cagatttcta
     3661 tttttttttt cttataagtt cgttgtgtct agagattgtt aatattgtaa tttaatgtag
     3721 acttactttg aataaaatta gtttaattgg ccttaaaatt acattaataa aactttgtga
     3781 tatgcaaatg acacattctt cataactctt tagcagcttt gactactagt gcactagaca
     3841 gaagtcagag cagggagcac ctgtcccctc tcaagtgatt attctttcct ggccacctct
     3901 accacaaaat accagtgggg ccaggtagtg acaagtgggt cttttttact tggacgattc
     3961 gttgtgctgt gcctttgttt catttgagca cagtggctca cgcctgtagt cccagctatg
     4021 ggagggcaag gtgggaggat cgtttgagcc tgagttccag accatcctga gcaccatagg
     4081 gagatcctgt ttccatttta gtttatttat ttttaattga ggtggagtct tgctctgttg
     4141 cccaagctgg agtgcagtgg tgcaatctca gctcactgca ccctccgcct cctgggttca
     4201 agcagttctc ccacctcagt ctcccaagta gctgggatta caggcacccg ccatcatgcc
     4261 cagctaatat ttgtattttt gtagagacgg ggtttcacta tgttggccag actggtcttg
     4321 aactcctaac ctcaggtgat ctgcccgcct tggcctccca aagtgctggg attacaggcg
     4381 tgagccacca tgcccggcct gcctgtttct gttttataaa agaaaggaaa atctatttat
     4441 atctattctc ttatttagca atatttaatg ttttattgtt aaaggtcagt gtggtaaaac
     4501 aaatagtata gaagaaatca ttgaaaaaga aacagcactt agacagaaat gctgtaaaag
     4561 tggaaaggag aaggtgaaat tacttgcagg ttagatggtt gcatacccag gtcatctaag
     4621 gagcaaatga aaatctgtga ctgaagaaag aattcaccaa gttaaccaga cacaaaatta
     4681 acaaaaacat cagtagcttt cctataaata taggctataa tggagggtca ttttttattt
     4741 ttatttagag acagggtctt aactgtcacc tggactggag tgcagtggca tgatcatgtt
     4801 tcacggcagc ctctaccttc tgggctcaag cgatcctctc ttctcagcca cctgagtagc
     4861 tgggcctaca ggtgtacatc accacgccca gataattttt attgtatttt ttatagaggc
     4921 agtgtcttgc tatgttgcca aggctgttct tgtactcctg gcctcaagcg aacctcctac
     4981 ctcagcctcc ccaaatgctg ggattacagg catgagacac tgcacctgac cagaagtcat
     5041 tttttcaata gcaaccccca aaaaagataa aatacctagt aacaaatagg gaaacaccta
     5101 tgtgaaaggt attataacat tactaaggaa cacaggccct gaataagtga aagttctgtt
     5161 gattctcaca ttttgctata cagtatctta agatgtaaaa ccttccttta aaacacaagc
     5221 ggattctaaa gttaaaacag aaaagcaaat atgccagact agtcaaattg ttttaaattc
     5281 tgctgttttt agaacattgt aattgaaaca gcatgatgct ggccctttaa aaagattcct
     5341 ggaacaagaa agtccacaaa taagccaaat acacaggaac tcggcgtggc acaaaggggc
     5401 cgtgtgtgat caggaactgg aactttgcaa caactaccca tgctcagaag tttgggccac
     5461 cttgaaaaga gaaaagttgg attagactcc tttttatact aagggaagtt ccaagtggtc
     5521 aagtatttac gtgtaaaaaa atacatatat atgaaaccat aatcataaga tacttttttt
     5581 gtaaattgac tattattgta taaatttata gagtataaag tgatcttata aattatgcat
     5641 acattgtgga ctagtcaagc tagttacatc catcacctca aatacttaac atttttgtag
     5701 tgagaacatc tgaaatttac tcttagcaat tttgaaatgt acaatactca atttttgact
     5761 gtattcacca tgctgtacaa tagaactcag aaaaagaaaa atatagtcct cctttctaag
     5821 a
//
