LOCUS       NM_003591               4183 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens cullin 2 (CUL2), transcript variant 3, mRNA.
ACCESSION   NM_003591
VERSION     NM_003591.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Wang,T., Zhang,Q., Xu,Y., Yan,R., Pan,Y., Xuan,Y., Shen,M.,
            Chen,X., Zhu,H., Ke,X., Qu,Y. and Zhang,X.
  TITLE     TRAF2 associates with cullin neddylation complex assembly
  JOURNAL   FEBS J 291 (20), 4473-4488 (2024)
   PUBMED   38978293
  REMARK    GeneRIF: TRAF2 associates with cullin neddylation complex assembly.
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Jia,L., Zhang,X., Zhou,T., Xie,J., Jin,J., Zhang,D., Zhu,C. and
            Wan,R.
  TITLE     Comprehensive prognostic and immunological analysis of Cullin2 in
            pan-cancer and its identification in hepatocellular carcinoma
  JOURNAL   Aging (Albany NY) 16 (10), 8898-8921 (2024)
   PUBMED   38787355
  REMARK    GeneRIF: Comprehensive prognostic and immunological analysis of
            Cullin2 in pan-cancer and its identification in hepatocellular
            carcinoma.
REFERENCE   3  (bases 1 to 4183)
  AUTHORS   Li,Y., Wei,Y., Zhang,H., Bai,Y., Wang,X., Li,Q., Liu,Y., Wang,S.,
            Wang,J., Wen,S., Li,J. and Zhao,W.
  TITLE     MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis
            by silencing Cullin2 in vitro and in vivo
  JOURNAL   PeerJ 11, e15641 (2023)
   PUBMED   37397007
  REMARK    GeneRIF: MicroRNA-154-5p suppresses cervical carcinoma growth and
            metastasis by silencing Cullin2 in vitro and in vivo.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4183)
  AUTHORS   Fonseca,B.F. and Chakrabarti,L.A.
  TITLE     A close shave: How SARS-CoV-2 induces the loss of cilia
  JOURNAL   J Cell Biol 221 (7) (2022)
   PUBMED   35695891
  REMARK    GeneRIF: A close shave: How SARS-CoV-2 induces the loss of cilia.
REFERENCE   5  (bases 1 to 4183)
  AUTHORS   Meng,L., Zhang,Y., Wu,P., Li,D., Lu,Y., Shen,P., Yang,T., Shi,G.,
            Chen,Q., Yuan,H., Ge,W., Miao,Y., Tu,M. and Jiang,K.
  TITLE     CircSTX6 promotes pancreatic ductal adenocarcinoma progression by
            sponging miR-449b-5p and interacting with CUL2
  JOURNAL   Mol Cancer 21 (1), 121 (2022)
   PUBMED   35650603
  REMARK    GeneRIF: CircSTX6 promotes pancreatic ductal adenocarcinoma
            progression by sponging miR-449b-5p and interacting with CUL2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4183)
  AUTHORS   Wada,H., Yeh,E.T. and Kamitani,T.
  TITLE     Identification of NEDD8-conjugation site in human cullin-2
  JOURNAL   Biochem Biophys Res Commun 257 (1), 100-105 (1999)
   PUBMED   10092517
REFERENCE   7  (bases 1 to 4183)
  AUTHORS   Lonergan,K.M., Iliopoulos,O., Ohh,M., Kamura,T., Conaway,R.C.,
            Conaway,J.W. and Kaelin,W.G. Jr.
  TITLE     Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau
            tumor suppressor protein requires binding to complexes containing
            elongins B/C and Cul2
  JOURNAL   Mol Cell Biol 18 (2), 732-741 (1998)
   PUBMED   9447969
REFERENCE   8  (bases 1 to 4183)
  AUTHORS   Pause,A., Lee,S., Worrell,R.A., Chen,D.Y., Burgess,W.H.,
            Linehan,W.M. and Klausner,R.D.
  TITLE     The von Hippel-Lindau tumor-suppressor gene product forms a stable
            complex with human CUL-2, a member of the Cdc53 family of proteins
  JOURNAL   Proc Natl Acad Sci U S A 94 (6), 2156-2161 (1997)
   PUBMED   9122164
REFERENCE   9  (bases 1 to 4183)
  AUTHORS   Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M.
  TITLE     cul-1 is required for cell cycle exit in C. elegans and identifies
            a novel gene family
  JOURNAL   Cell 85 (6), 829-839 (1996)
   PUBMED   8681378
REFERENCE   10 (bases 1 to 4183)
  AUTHORS   Kibel,A., Iliopoulos,O., DeCaprio,J.A. and Kaelin,W.G. Jr.
  TITLE     Binding of the von Hippel-Lindau tumor suppressor protein to
            Elongin B and C
  JOURNAL   Science 269 (5229), 1444-1446 (1995)
   PUBMED   7660130
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC401936.1, BC009591.1, AL392046.11 and AI089201.1.
            
            On Jun 2, 2019 this sequence version replaced NM_003591.3.
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (c) is
            shorter at the N-terminus compared to isoform a. Variants 3 and 4
            both encode the same isoform (c).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005651.1, BC009591.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374749.8/ ENSP00000363881.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-525               DC401936.1         43-567
            526-2864            BC009591.1         551-2889
            2865-4163           AL392046.11        43632-44930         c
            4164-4183           AI089201.1         14-33               c
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p11.21"
     gene            1..4183
                     /gene="CUL2"
                     /note="cullin 2"
                     /db_xref="GeneID:8453"
                     /db_xref="HGNC:HGNC:2552"
                     /db_xref="MIM:603135"
     exon            1..163
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            164..304
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..2423
                     /gene="CUL2"
                     /note="isoform c is encoded by transcript variant 3;
                     CUL-2; testis secretory sperm-binding protein Li 238E"
                     /codon_start=1
                     /product="cullin-2 isoform c"
                     /protein_id="NP_003582.2"
                     /db_xref="CCDS:CCDS7179.1"
                     /db_xref="GeneID:8453"
                     /db_xref="HGNC:HGNC:2552"
                     /db_xref="MIM:603135"
                     /translation="MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYA
                     LCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMD
                     CLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILI
                     RMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEY
                     YKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQ
                     FLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQEN
                     MPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLA
                     KYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMS
                     MDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQ
                     IYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKM
                     NYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMIN
                     HDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQA
                     AIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADE
                     YSYVA"
     exon            305..407
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            408..502
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            503..608
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            609..691
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            692..788
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            789..899
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1062
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1187
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1295
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1355
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1484
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1571
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1724
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1802
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1869
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..2072
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2174
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2291
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..4183
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2852..2857
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL2"
                     /note="hexamer: AATAAA"
     polyA_site      2881
                     /gene="CUL2"
                     /note="major polyA site"
     regulatory      2917..2922
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL2"
                     /note="hexamer: AATATA"
     polyA_site      2935
                     /gene="CUL2"
                     /note="major polyA site"
     regulatory      4163..4168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CUL2"
                     /note="hexamer: GATAAA"
     polyA_site      4183
                     /gene="CUL2"
                     /note="major polyA site"
ORIGIN      
        1 ctcggcgcac agtaatggca gcgctgtgag caggggaacg cgagctgaca gccgccgccg
       61 ccgccgcctc cgcccacctt cctcgccggg gcttcgtctt tcactccttc gggctgcctc
      121 cccctcccct tgtcccctgc cccttgccct gcttctgcag aagatttcaa cactacactt
      181 gcacaatgtc tttgaaacca agagtagtag attttgatga aacatggaac aaacttttga
      241 cgacaataaa agccgtggtc atgttggaat acgtcgaaag agcaacatgg aatgaccgtt
      301 tctcagatat ctatgcttta tgtgtggcct atcctgaacc ccttggagaa agactttata
      361 cagaaactaa gatttttttg gaaaatcatg ttcggcattt gcataagaga gttttggagt
      421 cagaagaaca agtacttgtt atgtatcata ggtactggga agaatacagc aagggtgcag
      481 actatatgga ctgcttatat aggtatctca acacccagtt tattaaaaag aataaattaa
      541 cagaagcgga ccttcagtat ggctatggtg gtgtagatat gaatgaacca cttatggaaa
      601 taggagagct agcattggat atgtggagga aattgatggt tgaaccactt caggccatcc
      661 ttatccgaat gctgctccga gaaatcaaaa atgatcgtgg tggagaagac ccaaaccaga
      721 aagtaatcca tggggttatt aactcctttg ttcatgttga acagtataag aaaaaattcc
      781 ccttaaagtt ttatcaggaa atttttgagt ctccctttct gactgaaaca ggagagtatt
      841 acaaacaaga agcttcaaat ttattacaag aatcaaactg ctcacagtat atggaaaagg
      901 ttctaggtag attaaaagat gaagaaattc gatgtcgaaa atacctacat ccaagttcat
      961 atactaaggt gattcatgaa tgtcaacaac gaatggtagc agaccactta cagtttttac
     1021 atgcagaatg tcataatata attcgacaag agaaaaaaaa tgacatggca aatatgtacg
     1081 tcttactccg tgctgtgtcc actggtttac ctcatatgat tcaggagctg caaaaccaca
     1141 tccatgatga gggccttcga gcaaccagca accttactca ggaaaacatg ccaacactat
     1201 ttgtggagtc agttttggaa gtgcatggta aatttgttca gcttatcaac actgttttga
     1261 atggtgatca gcattttatg agtgcgttgg ataaggccct tacgtcagtt gtaaattaca
     1321 gagaacctaa gtctgtttgc aaagcacctg aactgcttgc taagtactgt gacaacttac
     1381 tgaagaagtc agcgaaaggg atgacagaga atgaagtgga agacaggctc acgagcttca
     1441 tcacagtgtt caaatacatt gatgacaagg acgtctttca aaagttctac gcaagaatgc
     1501 tggcaaaacg tttaattcat gggttatcca tgtctatgga ctctgaagaa gccatgatca
     1561 acaaattaaa gcaagcctgt ggttatgagt ttaccagcaa gctacatcgg atgtatacag
     1621 atatgagtgt cagcgctgat ctcaacaata agttcaacaa ttttatcaaa aaccaagaca
     1681 cagtaataga tttgggaatt agttttcaaa tatatgttct acaggctggt gcgtggcctc
     1741 ttactcaggc tccttcatct acgtttgcaa ttccccagga attagaaaaa agtgtacaga
     1801 tgtttgaatt attttatagc caacatttca gtggaaggaa acttacatgg ttacattatc
     1861 tgtgtacagg tgaagttaaa atgaactatt tgggcaaacc atatgtagcc atggttacaa
     1921 cataccaaat ggcagttctt cttgccttta acaacagtga aactgtcagt tataaagagc
     1981 ttcaggacag cactcagatg aatgaaaagg aactgacaaa aacaatcaaa tcattacttg
     2041 atgtgaaaat gattaaccat gattcagaaa aggaagatat tgatgcagaa tcttcgtttt
     2101 cattaaatat gaactttagc agtaaaagaa caaaatttaa aattactaca tcaatgcaga
     2161 aagacacacc acaagaaatg gagcagacta gaagtgcagt tgatgaggac cggaaaatgt
     2221 atctccaagc tgctatagtt cgtatcatga aagcacgaaa agtgcttcgg cacaatgccc
     2281 ttattcaaga ggtgattagc cagtcaagag ctaggtttaa tcccagtatc agcatgatta
     2341 agaagtgtat tgaagttctg atagacaaac aatacataga acgcagccag gcgtcggcag
     2401 atgaatacag ctacgtcgcg tgatgtcgct ctcctccagc gtggtgtgag aagatcattg
     2461 ccatcaccat ttggtgtgtt cctgtgggaa aaagcaggac tgtgcctcca taatttggtc
     2521 atttggcagc ccctgttttc tgctgtttac aacatcacca gtgccacgtc atgagcgtca
     2581 aagaaaatgc ctagagatat ttcaagctca tgtcattatg acatttctta aaactttatt
     2641 aaaagaatga gtgaagtatt gctgaaaagt ggaaattcgg ttgggtacca tgctttttct
     2701 ccccttcacg tttgcagttg atgtgtcttt tttttttttt ttaatgtatc ttaaaggaca
     2761 taaaatttaa aaacttaaat attgtaatat gacagataac ctaataattg tatctacatt
     2821 aaaatgacaa acatgatact gctgcttgtc aaataaaaaa aaaataaaga aatagaatgc
     2881 cttttttatg tggatggagt atcaggttga ccacaaaata tattgactca aagcagctaa
     2941 tgcatcttta gttgcgtttt tatctgaatg gtttaattca cttgtactcc tatttaaatc
     3001 ctacatgaaa aatgtctaga ttattgttct tgactgcata ggactgcatt cagcataaag
     3061 aatgctttat ttttatggat tagatatatt ggatctaaac attttgaatc ttgaagatgt
     3121 aattccatca gcagtttctg gtggtgtgct actccacaga catcgcagag tgtgagcagg
     3181 atgcttggtg acctcaagtc tggcacagag agagcttttc attcaaaagt tgtctttctt
     3241 cggttgcata atccattaat tctagcatag actagtaccc tagctctgtg gccttccctg
     3301 agtcttagga aatctatgat accaacatat tccttctata tgcctcccct acctgttacc
     3361 cttacaaccc tcctccaaca gtttagatac tagagtcact ctcatcaatc acagatgtgc
     3421 ttagcaatgc ataacctaaa tactttttta aaaaagaaaa ttgtacattg tactgggtgc
     3481 cacatatata aatcccatta ttttgtttat tttatatata tatatatata taatatatat
     3541 atatatatat ctcaacagca gtgttaagag tactgcgatc tattatcata tttattgtct
     3601 atccacacca tcaccaccac caccacaccc ctcctccctc aacatacaat ttttctttat
     3661 tttaaaaaaa ataagagacg gggtttcgcc atgtttccca ggctagtctg gaacttctgg
     3721 cctcaagcaa tcctatctct gtctcccaaa gtgctgggat tacaagcatg agccactgca
     3781 tccatccaac acaaaatttt taaaatcgga atattttaaa gcaaatcaca caaattattt
     3841 cacttataat acttcagtaa ggcctttaaa aaatccacag tgatattatt actcctaaca
     3901 aaaacaataa ttacttagta tcatctaata tgtggttcat atttaaattt gttgttttga
     3961 gatgggtctt acaattggtt tattcaattg cattttttct aactcgtgtc tcaagtgttt
     4021 taaaaatcta ctgaacttat aatgacttat ataatgtatt tctcatttta cctttcttcc
     4081 aaaagaggaa ataatggcaa accatataat attgtacatt cactgtcaaa aagcaaaccc
     4141 ttgttttgat aacttgttga ttgataaaag ttttccaaat tga
//
