LOCUS       NM_005231               3249 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens cortactin (CTTN), transcript variant 1, mRNA.
ACCESSION   NM_005231
VERSION     NM_005231.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Chen,S., Duan,X., He,Y. and Chen,W.
  TITLE     METTL3 promotes osteogenic differentiation of human umbilical cord
            mesenchymal stem cells by up-regulating m6A modification of
            circCTTN
  JOURNAL   Biosci Rep 44 (3) (2024)
   PUBMED   38358895
  REMARK    GeneRIF: METTL3 promotes osteogenic differentiation of human
            umbilical cord mesenchymal stem cells by up-regulating m6A
            modification of circCTTN.
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Inoue,H., Kanda,T., Hayashi,G., Munenaga,R., Yoshida,M.,
            Hasegawa,K., Miyagawa,T., Kurumada,Y., Hasegawa,J., Wada,T.,
            Horiuchi,M., Yoshimatsu,Y., Itoh,F., Maemoto,Y., Arasaki,K.,
            Wakana,Y., Watabe,T., Matsushita,H., Harada,H. and Tagaya,M.
  TITLE     A MAP1B-cortactin-Tks5 axis regulates TNBC invasion and
            tumorigenesis
  JOURNAL   J Cell Biol 223 (3) (2024)
   PUBMED   38353696
  REMARK    GeneRIF: A MAP1B-cortactin-Tks5 axis regulates TNBC invasion and
            tumorigenesis.
REFERENCE   3  (bases 1 to 3249)
  AUTHORS   Kumar,R., Rottner,K. and Rao,G.N.
  TITLE     Requirement of Site-Specific Tyrosine Phosphorylation of Cortactin
            in Retinal Neovascularization and Vascular Leakage
  JOURNAL   Arterioscler Thromb Vasc Biol 44 (2), 366-390 (2024)
   PUBMED   38126170
  REMARK    GeneRIF: Requirement of Site-Specific Tyrosine Phosphorylation of
            Cortactin in Retinal Neovascularization and Vascular Leakage.
REFERENCE   4  (bases 1 to 3249)
  AUTHORS   Yun,S.I., Kwak,C., Lee,S.Y., Shin,S., Oh,C., Kim,J.S., Rhee,H.W.
            and Kim,K.K.
  TITLE     Binding of USP4 to cortactin enhances cell migration in HCT116
            human colon cancer cells
  JOURNAL   FASEB J 37 (5), e22900 (2023)
   PUBMED   37039823
  REMARK    GeneRIF: Binding of USP4 to cortactin enhances cell migration in
            HCT116 human colon cancer cells.
REFERENCE   5  (bases 1 to 3249)
  AUTHORS   Senguven Tokozlu,B., Sapkota,D., Vallenari,E.M., Schreurs,O. and
            Soland,T.M.
  TITLE     Cortactin expression in a Norwegian cohort of human papilloma virus
            negative oral squamous cell carcinomas of the mobile tongue
  JOURNAL   Eur J Oral Sci 131 (2), e12925 (2023)
   PUBMED   36790139
  REMARK    GeneRIF: Cortactin expression in a Norwegian cohort of human
            papilloma virus negative oral squamous cell carcinomas of the
            mobile tongue.
REFERENCE   6  (bases 1 to 3249)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
REFERENCE   7  (bases 1 to 3249)
  AUTHORS   Schuuring,E., Verhoeven,E., Litvinov,S. and Michalides,R.J.
  TITLE     The product of the EMS1 gene, amplified and overexpressed in human
            carcinomas, is homologous to a v-src substrate and is located in
            cell-substratum contact sites
  JOURNAL   Mol Cell Biol 13 (5), 2891-2898 (1993)
   PUBMED   8474448
REFERENCE   8  (bases 1 to 3249)
  AUTHORS   Brookes,S., Lammie,G.A., Schuuring,E., de Boer,C., Michalides,R.,
            Dickson,C. and Peters,G.
  TITLE     Amplified region of chromosome band 11q13 in breast and squamous
            cell carcinomas encompasses three CpG islands telomeric of FGF3,
            including the expressed gene EMS1
  JOURNAL   Genes Chromosomes Cancer 6 (4), 222-231 (1993)
   PUBMED   7685625
REFERENCE   9  (bases 1 to 3249)
  AUTHORS   Wu,H. and Parsons,J.T.
  TITLE     Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous
            actin-binding protein enriched in the cell cortex
  JOURNAL   J Cell Biol 120 (6), 1417-1426 (1993)
   PUBMED   7680654
REFERENCE   10 (bases 1 to 3249)
  AUTHORS   Schuuring,E., Verhoeven,E., Mooi,W.J. and Michalides,R.J.
  TITLE     Identification and cloning of two overexpressed genes, U21B31/PRAD1
            and EMS1, within the amplified chromosome 11q13 region in human
            carcinomas
  JOURNAL   Oncogene 7 (2), 355-361 (1992)
   PUBMED   1532244
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA716772.1, AK222613.1,
            AK291097.1 and BC008799.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005231.3.
            
            Summary: This gene is overexpressed in breast cancer and squamous
            cell carcinomas of the head and neck. The encoded protein is
            localized in the cytoplasm and in areas of the cell-substratum
            contacts. This gene has two roles: (1) regulating the interactions
            between components of adherens-type junctions and (2) organizing
            the cytoskeleton and cell adhesion structures of epithelia and
            carcinoma cells. During apoptosis, the encoded protein is degraded
            in a caspase-dependent manner. The aberrant regulation of this gene
            contributes to tumor cell invasion and metastasis. Three splice
            variants that encode different isoforms have been identified for
            this gene. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) is the longest transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ900949.1, SRR3476690.713548.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000301843.13/ ENSP00000301843.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DA716772.1         24-31
            9-1348              AK222613.1         1-1340
            1349-1926           AK291097.1         1232-1809
            1927-1950           AK222613.1         1919-1942
            1951-3249           BC008799.2         1825-3123
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.3"
     gene            1..3249
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /note="cortactin"
                     /db_xref="GeneID:2017"
                     /db_xref="HGNC:HGNC:3338"
                     /db_xref="MIM:164765"
     exon            1..86
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            87..183
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..1836
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /note="isoform a is encoded by transcript variant 1;
                     oncogene EMS1; ems1 sequence (mammary tumor and squamous
                     cell carcinoma-associated (p80/85 src substrate);
                     amplaxin; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="src substrate cortactin isoform a"
                     /protein_id="NP_005222.2"
                     /db_xref="CCDS:CCDS41680.1"
                     /db_xref="GeneID:2017"
                     /db_xref="HGNC:HGNC:3338"
                     /db_xref="MIM:164765"
                     /translation="MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTV
                     QGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGH
                     EYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGG
                     KYGVQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGFEYQGK
                     TEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQ
                     SERQDSAAVGFDYKEKLAKHESQQDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAY
                     QKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLE
                     EQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEA
                     ADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGD
                     DEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ"
     exon            184..270
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            271..344
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            345..474
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            475..585
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            586..640
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            641..751
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            752..862
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            863..973
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1084
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1140
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1210
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1359
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1449
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1627
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1699
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..3249
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3230..3235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /note="hexamer: AATAAA"
     polyA_site      3249
                     /gene="CTTN"
                     /gene_synonym="EMS1"
                     /note="major polyA site"
ORIGIN      
        1 ggaaccggaa gtagagcctg gtgcctggga gcggctggcg cggcggaatc cagggccgac
       61 ccgggccgga ccgaccccag gcggcgacgg aatcagtccc caatgcctgg aaattcctca
      121 ttggattact gtgttttaaa cagaatttcg tgaacagcct tttatctcca agcggaaaga
      181 aagatgtgga aagcttcagc aggccacgct gtgtccatcg cccaggatga cgcgggggcc
      241 gatgactggg agaccgaccc tgattttgtg aatgatgtga gtgagaagga gcaaagatgg
      301 ggtgccaaga cggtgcaggg ctccgggcac caggagcata tcaacataca caagctgagg
      361 gagaatgtct ttcaagagca tcagaccctt aaggagaagg aacttgaaac aggaccaaaa
      421 gcttcccatg gctatggagg gaaatttggt gtggaacaag accgaatgga taagtcagct
      481 gtcggccacg aatatcagtc gaaactttcc aagcactgct cgcaggtgga ctcggtccgt
      541 ggcttcggag gcaagtttgg tgtccagatg gacagagttg atcagtctgc tgtaggcttt
      601 gaataccagg ggaagactga gaagcatgcc tcccagaaag actactccag tggttttggc
      661 ggcaagtatg gcgtgcaggc cgaccgagta gacaagagcg cggtgggctt cgactaccag
      721 ggcaagacgg agaagcacga gtcacagaga gattactcca aaggtttcgg cggcaaatac
      781 ggtatcgaca aggacaaagt ggataagagc gccgttggct ttgagtatca aggcaaaacg
      841 gagaagcacg agtcccagaa agactatgtg aaagggtttg gaggaaaatt tggtgtgcag
      901 acagacagac aagacaaatg tgcccttggc tgggatcacc aggagaaatt gcagctgcat
      961 gaatcccaaa aagattataa gactggtttt ggaggcaaat tcggtgttca gtcggagagg
     1021 caggactccg ctgctgtggg gtttgattac aaggagaagc tggccaagca cgagtcccag
     1081 caagactact ccaaaggatt cggcgggaag tatggggtgc agaaggatcg gatggataag
     1141 aatgcgtcaa cctttgagga tgtcacccag gtgtcctctg cctaccagaa gacagtacct
     1201 gtcgaagctg tgaccagcaa aacaagtaac atcagagcta actttgaaaa cctcgctaag
     1261 gagaaagagc aggaggacag gcggaaggcg gaggcggaga gagcccagcg gatggccaag
     1321 gagcggcagg agcaggaaga ggccaggagg aagctggagg agcaagccag agccaaaacg
     1381 caaacgcccc ctgtgtcgcc cgcacctcag ccaaccgagg agaggctgcc ctcgagcccc
     1441 gtctatgagg atgcggcttc cttcaaggca gagctgagct acagaggccc tgtgagtggg
     1501 acggagccgg agcccgtgta cagcatggag gccgctgact accgagaggc cagcagccag
     1561 cagggcctgg cctatgccac agaggctgtc tatgaaagcg cagaggcccc gggccactat
     1621 cccgcagagg acagcaccta cgatgagtac gagaacgatc tggggatcac agccgtcgcc
     1681 ctgtacgact accaggctgc gggcgatgat gagatctcat ttgaccctga tgacatcatc
     1741 accaacatcg agatgattga cgacggctgg tggcgcgggg tgtgcaaggg ccggtacggg
     1801 ctcttcccag ccaactatgt ggagctgcgg cagtagggcc cccagccccc ccccggagct
     1861 gcgccctgga tcctcacact acagatcagg ccttctttgg ttcttgggtg gttttgggtt
     1921 ttttctgttt tttttttttt tttttttttt ttgaaggtgg ggaggggaat atacacattg
     1981 cttttatatt taatactttt gctgatgctt ttgaaaatgt ttatgccaca gaatttgcta
     2041 atatattgta atcacattcc ttaggaggac tttggtaatt ggttttatgc attgatggtt
     2101 ttttttttct tttttgccaa attgactgtc acgcggcagc ttcagggagc tcgcattctc
     2161 ttgtgttcgt gttgccctcg tgcccatcaa gtgcagtcgg gacctcccag gacaagcacg
     2221 aggcctcagg tcggccctgt ggcgggtagg caggaaggac tgtcccagac gaggggcttc
     2281 ctctagagtc tcactgctgg ggaggagagg actgggcctg atggaagtta acccggagct
     2341 aagtcaccca gagcacagga gctgccatgt cagatgggaa atctgcctat gtcataccgt
     2401 gacagcccgc aggatcaggt gacttctagc agagaccctg gtttttttcc tgtgcccact
     2461 ccggcttgtc ctcatctcta cccatcccct gatgcccagg tcaccgggag ggctgctggg
     2521 agcctctcct gtccccgccg ggcagtgtca ctgagtcctt gaaatcctcc cctgccccgc
     2581 gggtctctgg attgggacgc acagtgcagt tgaggtctgc gtcgggcttg gcttttcaca
     2641 aaggctgatg tcttaactgt cacccatatg gtccctgggc caccgggcag cctggggcgg
     2701 tgtgtgtgcc atgtcacagc atggcctctc ggccttggga aggaaggcag tgcctgctct
     2761 gctgtgagcc gccaggaacc ctcctcctgt caatgggggt gtagtatttt tgccaaaata
     2821 tcatgttcaa tttcagtagt ttgatcagtt gaaggctaga agtgtgaagt gcagatgagt
     2881 gtgtgttctt ccccaaggtc cccccacagc tccaggacac cgctgtcctg gcatttgtgg
     2941 ccactcactt tgtaggaaac tcatctcctt cctgaggagc cgggaggctg gaccagtccc
     3001 gtcgtgcagt caggtgggcg gtgtgtcttt ccagaaggtc acgtggaaat gtctcgggac
     3061 ttgggtcccg gagtgcccgt gaagcgtgtt tttgctcctg aggtgcattt tctcatcatc
     3121 cttgctttac cacaatgagc aatgaggtcg ggttttatat gcaacttatt gtatctgaat
     3181 tcctgtagca cacctcatag gtatgatttt tttaaattaa agaattcaga ataaacattt
     3241 tttgatcca
//
