LOCUS       NM_001912               1654 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens cathepsin L (CTSL), transcript variant 1, mRNA.
ACCESSION   NM_001912
VERSION     NM_001912.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Bestle,D., Bittel,L., Werner,A.D., Kamper,L., Dolnik,O.,
            Krahling,V., Steinmetzer,T. and Bottcher-Friebertshauser,E.
  TITLE     Novel proteolytic activation of Ebolavirus glycoprotein GP by
            TMPRSS2 and cathepsin L at an uncharted position can compensate for
            furin cleavage
  JOURNAL   Virus Res 347, 199430 (2024)
   PUBMED   38964470
  REMARK    GeneRIF: Novel proteolytic activation of Ebolavirus glycoprotein GP
            by TMPRSS2 and cathepsin L at an uncharted position can compensate
            for furin cleavage.
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Wu,Y., Jiang,D., Liu,Q., Yan,S., Liu,X., Wu,T., Sun,W. and Li,G.
  TITLE     Cathepsin L induces cellular senescence by upregulating CUX1 and
            p16INK4a
  JOURNAL   Aging (Albany NY) 16 (13), 10749-10764 (2024)
   PUBMED   38944813
  REMARK    GeneRIF: Cathepsin L induces cellular senescence by upregulating
            CUX1 and p16[INK4a].
REFERENCE   3  (bases 1 to 1654)
  AUTHORS   Xu,J., Xu,W., Choi,J., Brhane,Y., Christiani,D.C., Kothari,J.,
            McKay,J., Field,J.K., Davies,M.P.A., Liu,G., Amos,C.I., Hung,R.J.
            and Briollais,L.
  TITLE     Large-scale whole exome sequencing studies identify two genes,CTSL
            and APOE, associated with lung cancer
  JOURNAL   PLoS Genet 19 (9), e1010902 (2023)
   PUBMED   37738239
  REMARK    GeneRIF: Large-scale whole exome sequencing studies identify two
            genes,CTSL and APOE, associated with lung cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1654)
  AUTHORS   Liu,P., Ju,M., Zheng,X., Jiang,Y., Yu,X., Pan,B., Luo,R., Jia,W.
            and Zheng,M.
  TITLE     Methyltransferase-like 3 promotes cervical cancer metastasis by
            enhancing cathepsin L mRNA stability in an
            N6-methyladenosine-dependent manner
  JOURNAL   Cancer Sci 114 (3), 837-854 (2023)
   PUBMED   36382580
  REMARK    GeneRIF: Methyltransferase-like 3 promotes cervical cancer
            metastasis by enhancing cathepsin L mRNA stability in an
            N6-methyladenosine-dependent manner.
REFERENCE   5  (bases 1 to 1654)
  AUTHORS   Cui,K., Yao,S., Liu,B., Sun,S., Gong,L., Li,Q., Fei,B. and Huang,Z.
  TITLE     A novel high-risk subpopulation identified by CTSL and ZBTB7B in
            gastric cancer
  JOURNAL   Br J Cancer 127 (8), 1450-1460 (2022)
   PUBMED   35941174
  REMARK    GeneRIF: A novel high-risk subpopulation identified by CTSL and
            ZBTB7B in gastric cancer.
REFERENCE   6  (bases 1 to 1654)
  AUTHORS   Goretzki,L., Schmitt,M., Mann,K., Calvete,J., Chucholowski,N.,
            Kramer,M., Gunzler,W.A., Janicke,F. and Graeff,H.
  TITLE     Effective activation of the proenzyme form of the urokinase-type
            plasminogen activator (pro-uPA) by the cysteine protease cathepsin
            L
  JOURNAL   FEBS Lett 297 (1-2), 112-118 (1992)
   PUBMED   1551416
REFERENCE   7  (bases 1 to 1654)
  AUTHORS   Cataldo,A.M., Paskevich,P.A., Kominami,E. and Nixon,R.A.
  TITLE     Lysosomal hydrolases of different classes are abnormally
            distributed in brains of patients with Alzheimer disease
  JOURNAL   Proc Natl Acad Sci U S A 88 (24), 10998-11002 (1991)
   PUBMED   1837142
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1992 Mar 15;89(6):2509]
REFERENCE   8  (bases 1 to 1654)
  AUTHORS   Dunn,A.D., Crutchfield,H.E. and Dunn,J.T.
  TITLE     Thyroglobulin processing by thyroidal proteases. Major sites of
            cleavage by cathepsins B, D, and L
  JOURNAL   J Biol Chem 266 (30), 20198-20204 (1991)
   PUBMED   1939080
REFERENCE   9  (bases 1 to 1654)
  AUTHORS   Stearns,N.A., Dong,J.M., Pan,J.X., Brenner,D.A. and Sahagian,G.G.
  TITLE     Comparison of cathepsin L synthesized by normal and transformed
            cells at the gene, message, protein, and oligosaccharide levels
  JOURNAL   Arch Biochem Biophys 283 (2), 447-457 (1990)
   PUBMED   2275556
REFERENCE   10 (bases 1 to 1654)
  AUTHORS   Smith,C.G., Smith,M.T., Besch,N.F., Smith,R.G. and Asch,R.H.
  TITLE     Effect of delta 9-tetrahydrocannabinol (THC) on female reproductive
            function
  JOURNAL   Adv Biosci 22-23, 449-467 (1978)
   PUBMED   116880
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DN993009.1, AL832167.1,
            BM680778.1 and AL160279.21.
            
            On Nov 22, 2018 this sequence version replaced NM_001912.4.
            
            Summary: The protein encoded by this gene is a lysosomal cysteine
            proteinase that plays a major role in intracellular protein
            catabolism. Its substrates include collagen and elastin, as well as
            alpha-1 protease inhibitor, a major controlling element of
            neutrophil elastase activity. The encoded protein has been
            implicated in several pathologic processes, including myofibril
            necrosis in myopathies and in myocardial ischemia, and in the renal
            tubular response to proteinuria. This protein, which is a member of
            the peptidase C1 family, is a dimer composed of disulfide-linked
            heavy and light chains, both produced from a single protein
            precursor. Additionally, this protein cleaves the S1 subunit of the
            SARS-CoV-2 spike protein, which is necessary for entry of the virus
            into the cell. [provided by RefSeq, Aug 2020].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-4 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X12451.1, SRR3476690.922332.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in SARS-CoV-2 infection
            MANE Ensembl match     :: ENST00000343150.10/ ENSP00000345344.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-280               DN993009.1         67-346
            281-1576            AL832167.1         139-1434
            1577-1650           BM680778.1         21-94               c
            1651-1651           AL160279.21        91641-91641
            1652-1654           BM680778.1         17-19               c
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q21.33"
     gene            1..1654
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /note="cathepsin L"
                     /db_xref="GeneID:1514"
                     /db_xref="HGNC:HGNC:2537"
                     /db_xref="MIM:116880"
     exon            1..280
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     exon            281..416
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..1292
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /EC_number="3.4.22.15"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; major excreted protein; cathepsin L1;
                     procathepsin L"
                     /codon_start=1
                     /product="procathepsin L isoform 1 preproprotein"
                     /protein_id="NP_001903.1"
                     /db_xref="CCDS:CCDS6675.1"
                     /db_xref="GeneID:1514"
                     /db_xref="HGNC:HGNC:2537"
                     /db_xref="MIM:116880"
                     /translation="MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNE
                     EGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRK
                     GKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLIS
                     LSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYS
                     VANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDH
                     GVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV"
     exon            417..539
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     exon            540..686
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     exon            687..911
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1074
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1192
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1654
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1556..1561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /note="hexamer: AATAAA"
     polyA_site      1578
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /note="major polyA site"
     regulatory      1623..1628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
                     /note="hexamer: AATAAA"
     polyA_site      1654
                     /gene="CTSL"
                     /gene_synonym="CATL; CTSL1; MEP"
ORIGIN      
        1 agaaccgcga cctccgcaac cttgagcggc atccgtggag tgcgcctgcg cagctacgac
       61 cgcagcagga aagcgccgcc ggccaggccc agctgtggcc ggacagggac tggaagagag
      121 gacgcggtcg agtaggtgtg caccagccct ggcaacgaga gcgtctaccc cgaactctgc
      181 tggccttgag gtggggaagc cggggagggc agttgaggac cccgcggagg cgcgtgactg
      241 gttgagcggg caggccagcc tccgagccgg gtggacacag gttttaaaac atgaatccta
      301 cactcatcct tgctgccttt tgcctgggaa ttgcctcagc tactctaaca tttgatcaca
      361 gtttagaggc acagtggacc aagtggaagg cgatgcacaa cagattatac ggcatgaatg
      421 aagaaggatg gaggagagca gtgtgggaga agaacatgaa gatgattgaa ctgcacaatc
      481 aggaatacag ggaagggaaa cacagcttca caatggccat gaacgccttt ggagacatga
      541 ccagtgaaga attcaggcag gtgatgaatg gctttcaaaa ccgtaagccc aggaagggga
      601 aagtgttcca ggaacctctg ttttatgagg cccccagatc tgtggattgg agagagaaag
      661 gctacgtgac tcctgtgaag aatcagggtc agtgtggttc ttgttgggct tttagtgcta
      721 ctggtgctct tgaaggacag atgttccgga aaactgggag gcttatctca ctgagtgagc
      781 agaatctggt agactgctct gggcctcaag gcaatgaagg ctgcaatggt ggcctaatgg
      841 attatgcttt ccagtatgtt caggataatg gaggcctgga ctctgaggaa tcctatccat
      901 atgaggcaac agaagaatcc tgtaagtaca atcccaagta ttctgttgct aatgacaccg
      961 gctttgtgga catccctaag caggagaagg ccctgatgaa ggcagttgca actgtggggc
     1021 ccatttctgt tgctattgat gcaggtcatg agtccttcct gttctataaa gaaggcattt
     1081 attttgagcc agactgtagc agtgaagaca tggatcatgg tgtgctggtg gttggctacg
     1141 gatttgaaag cacagaatca gataacaata aatattggct ggtgaagaac agctggggtg
     1201 aagaatgggg catgggtggc tacgtaaaga tggccaaaga ccggagaaac cattgtggaa
     1261 ttgcctcagc agccagctac cccactgtgt gagctggtgg acggtgatga ggaaggactt
     1321 gactggggat ggcgcatgca tgggaggaat tcatcttcag tctaccagcc cccgctgtgt
     1381 cggatacaca ctcgaatcat tgaagatccg agtgtgattt gaattctgtg atattttcac
     1441 actggtaaat gttacctcta ttttaattac tgctataaat aggtttatat tattgattca
     1501 cttactgact ttgcattttc gtttttaaaa ggatgtataa atttttacct gtttaaataa
     1561 aatttaattt caaatgtagt ggtggggctt ctttctattt ttgatgcact gaatttttgt
     1621 gtaataaaga acataattgg gctctaagcc ataa
//
