LOCUS       NP_787055                586 aa            linear   PRI 12-JUN-2025
DEFINITION  CTP synthase 2 [Homo sapiens].
ACCESSION   NP_787055
VERSION     NP_787055.1
DBSOURCE    REFSEQ: accession NM_175859.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 586)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (residues 1 to 586)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (residues 1 to 586)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (residues 1 to 586)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (residues 1 to 586)
  AUTHORS   Gou,K.M., Chang,C.C., Shen,Q.J., Sung,L.Y. and Liu,J.L.
  TITLE     CTP synthase forms cytoophidia in the cytoplasm and nucleus
  JOURNAL   Exp Cell Res 323 (1), 242-253 (2014)
   PUBMED   24503052
REFERENCE   6  (residues 1 to 586)
  AUTHORS   Tan,W.L., Bhattacharya,B., Loh,M., Balasubramanian,I., Akram,M.,
            Dong,D., Wong,L., Thakkar,B., Salto-Tellez,M., Soo,R.A.,
            Fichtner,I., Iacopetta,B. and Soong,R.
  TITLE     Low cytosine triphosphate synthase 2 expression renders resistance
            to 5-fluorouracil in colorectal cancer
  JOURNAL   Cancer Biol Ther 11 (6), 599-608 (2011)
   PUBMED   21378502
  REMARK    GeneRIF: Low CTPS2 expression may be a rationally-based determinant
            of 5FU resistance.
REFERENCE   7  (residues 1 to 586)
  AUTHORS   Gamazon,E.R., Im,H.K., O'Donnell,P.H., Ziliak,D., Stark,A.L.,
            Cox,N.J., Dolan,M.E. and Huang,R.S.
  TITLE     Comprehensive evaluation of the contribution of X chromosome genes
            to platinum sensitivity
  JOURNAL   Mol Cancer Ther 10 (3), 472-480 (2011)
   PUBMED   21252287
  REMARK    GeneRIF: CTPS2 was identified by genome-wide gene expression
            analyses as correlated with cellular sensitivity to cisplatin and
            carboplatin.
REFERENCE   8  (residues 1 to 586)
  AUTHORS   Kassel,K.M., Au da,R., Higgins,M.J., Hines,M. and Graves,L.M.
  TITLE     Regulation of human cytidine triphosphate synthetase 2 by
            phosphorylation
  JOURNAL   J Biol Chem 285 (44), 33727-33736 (2010)
   PUBMED   20739275
  REMARK    GeneRIF: analysis of the kinetic properties of hCTPS1 and hCTPS2,
            and determination that Ser(68) is a major site of CTPS2 regulation
            by phosphorylation
REFERENCE   9  (residues 1 to 586)
  AUTHORS   Han,G.S., Sreenivas,A., Choi,M.G., Chang,Y.F., Martin,S.S.,
            Baldwin,E.P. and Carman,G.M.
  TITLE     Expression of Human CTP synthetase in Saccharomyces cerevisiae
            reveals phosphorylation by protein kinase A
  JOURNAL   J Biol Chem 280 (46), 38328-38336 (2005)
   PUBMED   16179339
REFERENCE   10 (residues 1 to 586)
  AUTHORS   van Kuilenburg,A.B., Meinsma,R., Vreken,P., Waterham,H.R. and van
            Gennip,A.H.
  TITLE     Identification of a cDNA encoding an isoform of human CTP
            synthetase
  JOURNAL   Biochim Biophys Acta 1492 (2-3), 548-552 (2000)
   PUBMED   10899599
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL445467.6 and AC073909.25.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: The protein encoded by this gene catalyzes the formation
            of CTP from UTP with the concomitant deamination of glutamine to
            glutamate. This protein is the rate-limiting enzyme in the
            synthesis of cytosine nucleotides, which play an important role in
            various metabolic processes and provide the precursors necessary
            for the synthesis of RNA and DNA. Cancer cells that exhibit
            increased cell proliferation also exhibit an increased activity of
            this encoded protein. Thus, this protein is an attractive target
            for selective chemotherapy. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 3. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034986.1, SRR1803615.3917.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000359276.9/ ENSP00000352222.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.2"
     Protein         1..586
                     /product="CTP synthase 2"
                     /EC_number="6.3.4.2"
                     /note="CTP synthetase type 2; cytidine 5'-triphosphate
                     synthetase 2; UTP-ammonia ligase 2; CTP synthase II"
                     /calculated_mol_wt=65547
     Region          1..557
                     /region_name="PLN02327"
                     /note="CTP synthase"
                     /db_xref="CDD:215186"
     Region          563..586
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NRF8.1)"
     Site            568
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
                     ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569;
                     propagated from UniProtKB/Swiss-Prot (Q9NRF8.1)"
     Site            571
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17081983,
                     ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163,
                     ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q9NRF8.1)"
     Site            574
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163,
                     ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q9NRF8.1)"
     CDS             1..586
                     /gene="CTPS2"
                     /gene_synonym="GATD5B"
                     /coded_by="NM_175859.3:375..2135"
                     /db_xref="CCDS:CCDS14175.1"
                     /db_xref="GeneID:56474"
                     /db_xref="HGNC:HGNC:2520"
                     /db_xref="MIM:300380"
ORIGIN      
        1 mkyilvtggv isgigkgiia ssigtilksc glrvtaikid pyinidagtf spyehgevfv
       61 lndggevdld lgnyerfldi nlykdnnitt gkiyqhvink errgdylgkt vqvvphitda
      121 vqewvmnqak vpvdgnkeep qicvielggt igdiegmpfv eafrqfqfka krenfcnihv
      181 slvpqlsatg eqktkptqns vralrglgls pdlivcrsst piemavkeki smfchvnpeq
      241 vicihdvsst yrvpvlleeq sivkyfkerl hlpigdsasn llfkwrnmad ryerlqkics
      301 ialvgkytkl rdcyasvfka lehsalainh klnlmyidsi dlekiteted pvkfheawqk
      361 lckadgilvp ggfgirgtlg klqaiswart kkipflgvcl gmqlaviefa rnclnlkdad
      421 stefrpnapv plvidmpehn pgnlggtmrl girrtvfkte nsilrklygd vpfieerhrh
      481 rfevnpnlik qfeqndlsfv gqdvdgdrme iielanhpyf vgvqfhpefs srpmkpsppy
      541 lglllaatgn lnaylqqgck lsssdrysda sddsfsepri aeleis
//
