LOCUS       NM_001326               2812 bp    mRNA    linear   PRI 15-JUL-2024
DEFINITION  Homo sapiens cleavage stimulation factor subunit 3 (CSTF3),
            transcript variant 1, mRNA.
ACCESSION   NM_001326
VERSION     NM_001326.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Luo,X., Wei,Q., Jiang,X., Chen,N., Zuo,X., Zhao,H., Liu,Y., Liu,X.,
            Xie,L., Yang,Y., Liu,T., Yi,P. and Xu,J.
  TITLE     CSTF3 contributes to platinum resistance in ovarian cancer through
            alternative polyadenylation of lncRNA NEAT1 and generating the
            short isoform NEAT1_1
  JOURNAL   Cell Death Dis 15 (6), 432 (2024)
   PUBMED   38898019
  REMARK    GeneRIF: CSTF3 contributes to platinum resistance in ovarian cancer
            through alternative polyadenylation of lncRNA NEAT1 and generating
            the short isoform NEAT1_1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Grozdanov,P.N., Masoumzadeh,E., Latham,M.P. and MacDonald,C.C.
  TITLE     The structural basis of CstF-77 modulation of cleavage and
            polyadenylation through stimulation of CstF-64 activity
  JOURNAL   Nucleic Acids Res 46 (22), 12022-12039 (2018)
   PUBMED   30257008
  REMARK    GeneRIF: Reverse genetics and nuclear magnetic resonance studies of
            recombinant CstF-64 (RRM-Hinge) and CstF-77
            (monkeytail-carboxy-terminal domain) indicate that the last 30
            amino acids of CstF-77 increases the stability of the RRM, thus
            altering the affinity of the complex for RNA. These results provide
            new insights into the mechanism by which CstF regulates the
            location of the RNA cleavage site during Cleavage/polyadenylation.
REFERENCE   3  (bases 1 to 2812)
  AUTHORS   Yang,W., Hsu,P.L., Yang,F., Song,J.E. and Varani,G.
  TITLE     Reconstitution of the CstF complex unveils a regulatory role for
            CstF-50 in recognition of 3'-end processing signals
  JOURNAL   Nucleic Acids Res 46 (2), 493-503 (2018)
   PUBMED   29186539
  REMARK    GeneRIF: Data show that the recruitment of CstF subunit CstF-50 to
            CstF via interaction with cleavage stimulation factor, 3' pre-RNA,
            subunit 3, 77kDa protein (CstF-77) and establish that the hexameric
            assembly of CstF.
REFERENCE   4  (bases 1 to 2812)
  AUTHORS   Hasegawa,Y., Taylor,D., Ovchinnikov,D.A., Wolvetang,E.J., de
            Torrente,L. and Mar,J.C.
  TITLE     Variability of Gene Expression Identifies Transcriptional
            Regulators of Early Human Embryonic Development
  JOURNAL   PLoS Genet 11 (8), e1005428 (2015)
   PUBMED   26288249
  REMARK    GeneRIF: Results from a study on gene variability markers in
            early-stage human embryos shows that CSTF3 is a putative
            variability marker for the 3-day, 8-cell embryo stage.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2812)
  AUTHORS   Mick,E., McGough,J., Deutsch,C.K., Frazier,J.A., Kennedy,D. and
            Goldberg,R.J.
  TITLE     Genome-wide association study of proneness to anger
  JOURNAL   PLoS One 9 (1), e87257 (2014)
   PUBMED   24489884
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2812)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     Complex protein interactions within the human polyadenylation
            machinery identify a novel component
  JOURNAL   Mol Cell Biol 20 (5), 1515-1525 (2000)
   PUBMED   10669729
REFERENCE   7  (bases 1 to 2812)
  AUTHORS   Murthy,K.G. and Manley,J.L.
  TITLE     The 160-kD subunit of human cleavage-polyadenylation specificity
            factor coordinates pre-mRNA 3'-end formation
  JOURNAL   Genes Dev 9 (21), 2672-2683 (1995)
   PUBMED   7590244
REFERENCE   8  (bases 1 to 2812)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     A polyadenylation factor subunit is the human homologue of the
            Drosophila suppressor of forked protein
  JOURNAL   Nature 372 (6505), 471-474 (1994)
   PUBMED   7984242
REFERENCE   9  (bases 1 to 2812)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     A human polyadenylation factor is a G protein beta-subunit
            homologue
  JOURNAL   J Biol Chem 267 (33), 23471-23474 (1992)
   PUBMED   1358884
REFERENCE   10 (bases 1 to 2812)
  AUTHORS   Takagaki,Y., MacDonald,C.C., Shenk,T. and Manley,J.L.
  TITLE     The human 64-kDa polyadenylylation factor contains a
            ribonucleoprotein-type RNA binding domain and unusual auxiliary
            motifs
  JOURNAL   Proc Natl Acad Sci U S A 89 (4), 1403-1407 (1992)
   PUBMED   1741396
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG435786.1, U15782.1 and
            BC010533.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001326.2.
            
            Summary: The protein encoded by this gene is one of three
            (including CSTF1 and CSTF2) cleavage stimulation factors that
            combine to form the cleavage stimulation factor complex (CSTF).
            This complex is involved in the polyadenylation and 3' end cleavage
            of pre-mRNAs. The encoded protein functions as a homodimer and
            interacts directly with both CSTF1 and CSTF2 in the CSTF complex.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.11290.1,
                                           SRR1660805.258395.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323959.9/ ENSP00000315791.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                BG435786.1         13-36
            25-2790             U15782.1           1-2766
            2791-2812           BC010533.1         1234-1255
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p13"
     gene            1..2812
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /note="cleavage stimulation factor subunit 3"
                     /db_xref="GeneID:1479"
                     /db_xref="HGNC:HGNC:2485"
                     /db_xref="MIM:600367"
     exon            1..182
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..2309
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /note="isoform 1 is encoded by transcript variant 1; CSTF
                     77 kDa subunit; CF-1 77 kDa subunit; cleavage stimulation
                     factor 77 kDa subunit; cleavage stimulation factor, 3'
                     pre-RNA, subunit 3, 77kD; cleavage stimulation factor, 3'
                     pre-RNA, subunit 3, 77kDa"
                     /codon_start=1
                     /product="cleavage stimulation factor subunit 3 isoform 1"
                     /protein_id="NP_001317.1"
                     /db_xref="CCDS:CCDS7883.1"
                     /db_xref="GeneID:1479"
                     /db_xref="HGNC:HGNC:2485"
                     /db_xref="MIM:600367"
                     /translation="MSGDGATEQAAEYVPEKVKKAEKKLEENPYDLDAWSILIREAQN
                     QPIDKARKTYERLVAQFPSSGRFWKLYIEAEIKAKNYDKVEKLFQRCLMKVLHIDLWK
                     CYLSYVRETKGKLPSYKEKMAQAYDFALDKIGMEIMSYQIWVDYINFLKGVEAVGSYA
                     ENQRITAVRRVYQRGCVNPMINIEQLWRDYNKYEEGINIHLAKKMIEDRSRDYMNARR
                     VAKEYETVMKGLDRNAPSVPPQNTPQEAQQVDMWKKYIQWEKSNPLRTEDQTLITKRV
                     MFAYEQCLLVLGHHPDIWYEAAQYLEQSSKLLAEKGDMNNAKLFSDEAANIYERAIST
                     LLKKNMLLYFAYADYEESRMKYEKVHSIYNRLLAIEDIDPTLVYIQYMKFARRAEGIK
                     SGRMIFKKAREDTRTRHHVYVTAALMEYYCSKDKSVAFKIFELGLKKYGDIPEYVLAY
                     IDYLSHLNEDNNTRVLFERVLTSGSLPPEKSGEIWARFLAFESNIGDLASILKVEKRR
                     FTAFKEEYEGKETALLVDRYKFMDLYPCSASELKALGYKDVSRAKLAAIIPDPVVAPS
                     IVPVLKDEVDRKPEYPKPDTQQMIPFQPRHLAPPGLHPVPGGVFPVPPAAVVLMKLLP
                     PPICFQGPFVQVDELMEIFRRCKIPNTVEEAVRIITGGAPELAVEGNGPVESNAVLTK
                     AVKRPNEDSDEDEEKGAVVPPVHDIYRARQQKRIR"
     exon            183..284
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            285..380
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            381..413
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            414..511
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            512..578
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            579..613
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            614..740
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            741..818
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            819..981
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1091
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1208
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1283
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1427
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1530
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1600
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1796
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1950
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2045
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2106
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2812
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2793..2798
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /note="hexamer: AATAAA"
     polyA_site      2812
                     /gene="CSTF3"
                     /gene_synonym="CSTF-77"
                     /note="major polyA site"
ORIGIN      
        1 aattcatatt tgagtgggcg gtggcgattg gtgttggcgg tctggctcag ctgggcaggg
       61 ggtaacttta ctgatttggg ggtggttttt agtttaattt ttcttttcta gcttcccatc
      121 gacggtcagt gcgcacgttg taatcagctg aggccatgtc aggagacgga gccacggagc
      181 aggcagctga gtatgtccca gagaaggtga agaaagcgga aaagaaatta gaagagaatc
      241 catatgacct tgatgcttgg agcattctca ttcgagaggc acagaatcaa cctatagaca
      301 aagcacggaa gacttatgaa cgccttgttg cccagttccc cagttctggc agattctgga
      361 aactgtacat tgaagcagag attaaagcta aaaattatga caaggttgaa aagctatttc
      421 agagatgcct tatgaaggtt ttgcacattg atttatggaa gtgttatctt tcatatgtcc
      481 gagaaaccaa gggtaaacta ccaagttaca aagaaaaaat ggctcaagca tatgactttg
      541 cactggataa aattggaatg gaaattatgt cctatcagat ttgggtggat tacatcaatt
      601 tcctaaaagg cgtggaagct gtaggatctt atgcagaaaa tcaaagaata acagctgtcc
      661 gaagagttta tcaacgaggt tgtgttaatc cgatgatcaa cattgaacag ctctggagag
      721 actataacaa gtatgaagag ggtatcaata ttcatttagc taaaaaaatg attgaagatc
      781 ggagtagaga ttatatgaat gctagacgtg tagcaaagga atatgagaca gtaatgaaag
      841 gcttggaccg taatgctccc tcggtgcctc ctcagaatac tcctcaagaa gctcaacaag
      901 tagatatgtg gaagaaatat atacagtggg aaaagagcaa ccctcttcgt acagaggatc
      961 agacccttat aacaaaaaga gttatgtttg cttatgaaca gtgcctgctt gtgctgggcc
     1021 atcaccctga tatttggtat gaagctgccc agtatcttga gcagtcaagt aaactgctcg
     1081 cagaaaaggg agatatgaat aatgccaaat tatttagtga tgaagctgct aatatatatg
     1141 aaagagccat aagcacttta ttgaagaaga atatgcttct ttattttgca tatgcagatt
     1201 atgaagagag tcgcatgaag tatgaaaagg ttcacagtat atataacaga cttctggcaa
     1261 ttgaggatat tgaccctacc ttggtatata tccaatatat gaaatttgca cggagagcag
     1321 aaggcatcaa atctggaaga atgatattta aaaaagcaag agaagatacc agaacccgcc
     1381 accatgtcta tgttactgca gcactcatgg aatattactg tagtaaggac aaatctgttg
     1441 cctttaagat ttttgagctg gggctaaaaa aatatggaga cattccagag tatgtcctgg
     1501 cctatattga ctatctttct cacctcaatg aggacaataa tacccgagtt ttgtttgaac
     1561 gagttttaac atctggaagc cttcctcctg agaagtctgg agaaatctgg gcccgatttc
     1621 tagcatttga aagtaatatt ggtgatctag ctagtatact caaagtggag aaaagacggt
     1681 ttacagcatt caaagaagag tatgaaggga aagaaacggc tttactagta gatagataca
     1741 agttcatgga tttatatcct tgctctgcaa gtgaattaaa agcacttggt tataaggatg
     1801 tctcccgtgc taagctagca gctataattc cggacccagt tgtagctcct tctatagtgc
     1861 ctgttctgaa agatgaagtg gatagaaaac cagaataccc taaaccagac actcagcaga
     1921 tgattccatt tcagccacga catttagcac ctccaggttt acaccctgta cctggtggag
     1981 tgttcccagt ccctcctgca gctgttgttt taatgaaact tctccctcct cctatctgtt
     2041 tccagggtcc ttttgtacaa gtggatgaac tgatggaaat tttccgaaga tgcaagatac
     2101 caaatactgt tgaggaagct gtgaggatca ttactggtgg ggccccagag ctagctgtag
     2161 aaggcaacgg ccccgtggaa agtaatgcag tactcaccaa ggccgtcaaa aggcccaacg
     2221 aggattcaga tgaagatgaa gaaaagggag ccgttgtccc ccctgttcat gacatttaca
     2281 gagcacggca gcagaagcgg attcggtagg gttttaaacg cctctgcaga aaactcctgt
     2341 ccaggattcc ttttgcctca agtggtatgt ttaaaagaga caacgctttg ttacaaggtt
     2401 cttggaaaca aagttgtatt gtcattggtg cctctatcac atggttcttg agaaaaaaca
     2461 aaccaacctg tgtgaatttt agaatacgga acagacctat gctctaagca aaattaggtt
     2521 ttcaaaaatg tgagaacagt acaaagtggc agaaccacat tttgttccct cttcaagggt
     2581 gtcttgtatg tgccgcttga agatttgtga gtttttcaac agttttattt taaaaactgg
     2641 atggcttatg attgtaaagc attttatcac attttctgaa aacaattgtt cttggtttgc
     2701 ttatgtagag tcctgcctta ttgtttgttt ttatttatgg cagaatgtat gaaatccgtt
     2761 ttgtagtttc aaattttaaa agtcctttaa aaaataaaag gattcagaaa ca
//
