LOCUS       NM_001325               2570 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens cleavage stimulation factor subunit 2 (CSTF2),
            transcript variant 2, mRNA.
ACCESSION   NM_001325
VERSION     NM_001325.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Zheng,Y., Li,X., Deng,S., Zhao,H., Ye,Y., Zhang,S., Huang,X.,
            Bai,R., Zhuang,L., Zhou,Q., Li,M., Su,J., Li,R., Bao,X., Zeng,L.,
            Chen,R., Zheng,J., Lin,D., He,C., Zhang,J. and Zuo,Z.
  TITLE     CSTF2 mediated mRNA N6-methyladenosine modification drives
            pancreatic ductal adenocarcinoma m6A subtypes
  JOURNAL   Nat Commun 14 (1), 6334 (2023)
   PUBMED   37816727
  REMARK    GeneRIF: CSTF2 mediated mRNA N[6]-methyladenosine modification
            drives pancreatic ductal adenocarcinoma m[6]A subtypes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Tan,Y., Zheng,T., Zhang,R., Chen,S., Cheng,Q., Zhang,J., Wang,R.,
            Chen,M. and Na,N.
  TITLE     Alternative polyadenylation writer CSTF2 forms a positive loop with
            FGF2 to promote tubular epithelial-mesenchymal transition and renal
            fibrosis
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1868 (12), 166541 (2022)
   PUBMED   36113752
  REMARK    GeneRIF: Alternative polyadenylation writer CSTF2 forms a positive
            loop with FGF2 to promote tubular epithelial-mesenchymal transition
            and renal fibrosis.
REFERENCE   3  (bases 1 to 2570)
  AUTHORS   Zhang,M.H. and Liu,J.
  TITLE     Cleavage stimulation factor 2 promotes malignant progression of
            liver hepatocellular carcinoma by activating phosphatidylinositol
            3'-kinase/protein kinase B/mammalian target of rapamycin pathway
  JOURNAL   Bioengineered 13 (4), 10047-10060 (2022)
   PUBMED   35412944
  REMARK    GeneRIF: Cleavage stimulation factor 2 promotes malignant
            progression of liver hepatocellular carcinoma by activating
            phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of
            rapamycin pathway.
REFERENCE   4  (bases 1 to 2570)
  AUTHORS   Lin,A., Ji,P., Niu,X., Zhao,X., Chen,Y., Liu,W., Liu,Y., Fan,W.,
            Sun,Y., Miao,C., Zhang,S., Tan,W., Lin,D., Wagner,E.J. and Wu,C.
  TITLE     CstF64-Induced Shortening of the BID 3'UTR Promotes Esophageal
            Squamous Cell Carcinoma Progression by Disrupting ceRNA Cross-talk
            with ZFP36L2
  JOURNAL   Cancer Res 81 (22), 5638-5651 (2021)
   PUBMED   34607841
  REMARK    GeneRIF: CstF64-Induced Shortening of the BID 3'UTR Promotes
            Esophageal Squamous Cell Carcinoma Progression by Disrupting ceRNA
            Cross-talk with ZFP36L2.
REFERENCE   5  (bases 1 to 2570)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2570)
  AUTHORS   Ruegsegger,U., Beyer,K. and Keller,W.
  TITLE     Purification and characterization of human cleavage factor Im
            involved in the 3' end processing of messenger RNA precursors
  JOURNAL   J Biol Chem 271 (11), 6107-6113 (1996)
   PUBMED   8626397
REFERENCE   7  (bases 1 to 2570)
  AUTHORS   Murthy,K.G. and Manley,J.L.
  TITLE     The 160-kD subunit of human cleavage-polyadenylation specificity
            factor coordinates pre-mRNA 3'-end formation
  JOURNAL   Genes Dev 9 (21), 2672-2683 (1995)
   PUBMED   7590244
REFERENCE   8  (bases 1 to 2570)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     A polyadenylation factor subunit is the human homologue of the
            Drosophila suppressor of forked protein
  JOURNAL   Nature 372 (6505), 471-474 (1994)
   PUBMED   7984242
REFERENCE   9  (bases 1 to 2570)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     A human polyadenylation factor is a G protein beta-subunit
            homologue
  JOURNAL   J Biol Chem 267 (33), 23471-23474 (1992)
   PUBMED   1358884
REFERENCE   10 (bases 1 to 2570)
  AUTHORS   Takagaki,Y., MacDonald,C.C., Shenk,T. and Manley,J.L.
  TITLE     The human 64-kDa polyadenylylation factor contains a
            ribonucleoprotein-type RNA binding domain and unusual auxiliary
            motifs
  JOURNAL   Proc Natl Acad Sci U S A 89 (4), 1403-1407 (1992)
   PUBMED   1741396
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z95327.1, Z83819.1, M85085.1
            and AW016125.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_001325.2.
            
            Summary: This gene encodes a nuclear protein with an RRM (RNA
            recognition motif) domain. The protein is a member of the cleavage
            stimulation factor (CSTF) complex that is involved in the 3' end
            cleavage and polyadenylation of pre-mRNAs. Specifically, this
            protein binds GU-rich elements within the 3'-untranslated region of
            mRNAs. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the central coding region, compared to variant 1. The
            encoded isoform (2) is shorter, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M85085.1, BC017712.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372972.7/ ENSP00000362063.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                Z95327.1           40208-40291
            85-163              Z95327.1           41344-41422
            164-333             Z95327.1           42068-42237
            334-470             Z95327.1           43109-43245
            471-590             Z95327.1           43703-43822
            591-728             Z95327.1           43937-44074
            729-852             Z95327.1           46451-46574
            853-915             Z95327.1           47857-47919
            916-1057            Z95327.1           51332-51473
            1058-1233           Z95327.1           52551-52726
            1234-1526           Z95327.1           52997-53289
            1527-1637           Z83819.1           677-787
            1638-1763           Z83819.1           1577-1702
            1764-1948           Z83819.1           4052-4236
            1949-1982           M85085.1           1945-1978
            1983-1985           AW016125.1         15-17               c
            1986-2570           Z83819.1           4274-4858
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.1"
     gene            1..2570
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /note="cleavage stimulation factor subunit 2"
                     /db_xref="GeneID:1478"
                     /db_xref="HGNC:HGNC:2484"
                     /db_xref="MIM:300907"
     exon            1..84
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1760
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /note="isoform 2 is encoded by transcript variant 2; CF-1
                     64 kDa subunit; CSTF 64 kDa subunit; cleavage stimulation
                     factor 64 kDa subunit; cleavage stimulation factor, 3'
                     pre-RNA, subunit 2, 64kD; cleavage stimulation factor, 3'
                     pre-RNA, subunit 2, 64kDa"
                     /codon_start=1
                     /product="cleavage stimulation factor subunit 2 isoform 2"
                     /protein_id="NP_001316.1"
                     /db_xref="CCDS:CCDS14473.1"
                     /db_xref="GeneID:1478"
                     /db_xref="HGNC:HGNC:2484"
                     /db_xref="MIM:300907"
                     /translation="MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVS
                     FRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELK
                     SLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARN
                     MLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSN
                     VSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGG
                     GMQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTL
                     LSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPML
                     DQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAM
                     EARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTG
                     MQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILIL
                     KEQIQKSTGAP"
     exon            85..163
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            164..333
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            334..470
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            471..590
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            591..728
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            729..852
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            853..915
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1057
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1233
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1526
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1637
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1763
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..2570
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1951..1956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /note="hexamer: AATAAA"
     polyA_site      1985
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /note="major polyA site"
     regulatory      2548..2553
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
                     /note="hexamer: AATAAA"
     polyA_site      2570
                     /gene="CSTF2"
                     /gene_synonym="CstF-64; CSTF64; XLID113"
ORIGIN      
        1 gcaccggaag ccgactcaac agagctatgg cgggtttgac tgtgagagac ccagcggtgg
       61 atcgttctct acgttctgtg ttcgtgggga acattcctta tgaagctact gaagagcagt
      121 tgaaggacat cttttctgag gttggacctg ttgttagttt cagattggta tacgatagag
      181 agacaggaaa gccaaagggt tatggcttct gtgaatacca agaccaagag acagcactta
      241 gtgccatgcg gaacctgaat gggcgcgaat tcagtgggag agcacttcga gtggacaatg
      301 ctgccagtga aaagaacaaa gaagagctga agagccttgg cactggtgcc cctgtcattg
      361 agtcacctta tggagagacc atcagtcctg aggatgcccc tgagtccatt agcaaagcag
      421 ttgccagcct tccaccagag cagatgtttg agctgatgaa acaaatgaag ctctgtgtcc
      481 agaatagtcc ccaggaggca cggaacatgt tacttcagaa ccctcaactg gcttatgctt
      541 tgctgcaagc acaggtagtg atgagaattg tggatccgga aattgccctg aaaattctgc
      601 atcgccagac aaatatccca acgctgattg caggcaaccc tcagccagtc catggtgctg
      661 ggcctggctc aggatccaat gtgtcaatga accagcagaa tcctcaggcc cctcaggccc
      721 agtctttggg tggaatgcat gtcaatggcg cacctcctct gatgcaagct tctatgcagg
      781 gtggagttcc agcaccaggg caaatgccag ctgctgtcac aggacctggc cctggttcct
      841 tagctcctgg aggaggaatg caggctcagg ttggaatgcc aggaagtgga ccagtgtcca
      901 tggaacgggg gcaagtgccg atgcaagacc ccagagcagc tatgcagcgg ggatccttgc
      961 ctgcgaatgt cccaacccct cgaggcttgt taggagatgc tccgaatgat ccacggggag
     1021 gcactttact ttctgtaact ggagaggtag agcctagagg ttacttggga ccacctcatc
     1081 agggtccacc catgcaccat gtccctggcc atgagagccg aggaccaccc ccacatgaac
     1141 tgaggggagg gccattaccc gagcccagac ctctaatggc agaaccaaga ggacccatgc
     1201 tagatcagag gggtccaccc ttggatggca gaggtggaag ggatccccga ggaatagatg
     1261 cacgagggat ggaggcccga gccatggagg caagagggtt agatgccaga ggattagagg
     1321 cccgtgcaat ggaggcccgt gcgatggaag ctcgtgcaat ggaggcccga gcgatggagg
     1381 cccgtgcaat ggaagtccga gggatggagg ccagaggcat ggataccaga ggcccagtgc
     1441 ctggccccag aggacctata cctagtggaa tgcagggtcc cagtccaatt aacatggggg
     1501 cggttgtccc ccagggatcc agacaggtcc cagtcatgca gggaacagga atgcaaggag
     1561 caagtataca gggtggaagc cagcctggcg gctttagtcc cgggcagaac caagtcactc
     1621 cacaggatca tgagaaggct gctttgatta tgcaggttct acaactgact gcagaccaga
     1681 ttgccatgtt gcctcctgag caaaggcaga gtatcctgat tttaaaggaa caaatacaga
     1741 aatccactgg agcaccttga taggttttca aaaatacctg gcaagaaatc tggaaattct
     1801 ataattttgt tgaaatattg aaaaaagatg acctgcatcc taacccttga atgactcaaa
     1861 tcagtgccag gtggaggact cccatcacct tctctcagaa caaaatcact tcattttatt
     1921 gtcttagttt gtatattttc tgtgacttga aataaacttt gaacacaatt ttagtacact
     1981 gcaaattgat acacatgacc tttttgcttt taaaaagctt aacactaagg gtgaaacctt
     2041 agatgtgttt tctaccctta ctgcagtatt gtactttaac atattgccat agttttgttt
     2101 ttaagttaac cacttattct tcttgatgat atttgagaat ttctcagaag caatgttttc
     2161 tggaacttgg tttttgttta gtttgcttcc agtaagaatt attataaggc tttgccctct
     2221 gaaaatattc tagcacttcg gagtcttcta ggagaaaata tcacatgctt agagctataa
     2281 aatggtcaga aggctaacag agcaaagagc ctagcacagc tgtgtgttta gtttagaaaa
     2341 atccaaatat caaaatgcat ttgtttcaat agcaggtctg aacatgtata attcagagtt
     2401 aagacaaaaa ttgttttagc actggtttga gtgtgtcagc aacacaagag actgttgaca
     2461 tttacttgtg tgttcttaaa cttatcagtc taggcagtca actttgaaag tttaaaaagc
     2521 cactgatttt tcacatgctc aagatgaaat aaaaacagaa atcaaatgaa
//
