LOCUS       NM_001324               4032 bp    mRNA    linear   PRI 27-APR-2025
DEFINITION  Homo sapiens cleavage stimulation factor subunit 1 (CSTF1),
            transcript variant 2, mRNA.
ACCESSION   NM_001324
VERSION     NM_001324.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Tu,Y.X.I., Sydor,A.M., Coyaud,E., Laurent,E.M.N., Dyer,D.,
            Mellouk,N., St-Germain,J., Vernon,R.M., Forman-Kay,J.D., Li,T.,
            Hua,R., Zhao,K., Ridgway,N.D., Kim,P.K., Raught,B. and Brumell,J.H.
  TITLE     Global Proximity Interactome of the Human Macroautophagy Pathway
  JOURNAL   Autophagy 18 (5), 1174-1186 (2022)
   PUBMED   34524948
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4032)
  AUTHORS   Nicolai,S., Filippi,S., Caputo,M., Cipak,L., Gregan,J., Ammerer,G.,
            Frontini,M., Willems,D., Prantera,G., Balajee,A.S. and
            Proietti-De-Santis,L.
  TITLE     Identification of Novel Proteins Co-Purifying with Cockayne
            Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA
            Metabolism and Chromatin Dynamics
  JOURNAL   PLoS One 10 (6), e0128558 (2015)
   PUBMED   26030138
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4032)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   5  (bases 1 to 4032)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   6  (bases 1 to 4032)
  AUTHORS   Ruegsegger,U., Beyer,K. and Keller,W.
  TITLE     Purification and characterization of human cleavage factor Im
            involved in the 3' end processing of messenger RNA precursors
  JOURNAL   J Biol Chem 271 (11), 6107-6113 (1996)
   PUBMED   8626397
REFERENCE   7  (bases 1 to 4032)
  AUTHORS   Murthy,K.G. and Manley,J.L.
  TITLE     The 160-kD subunit of human cleavage-polyadenylation specificity
            factor coordinates pre-mRNA 3'-end formation
  JOURNAL   Genes Dev 9 (21), 2672-2683 (1995)
   PUBMED   7590244
REFERENCE   8  (bases 1 to 4032)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     A polyadenylation factor subunit is the human homologue of the
            Drosophila suppressor of forked protein
  JOURNAL   Nature 372 (6505), 471-474 (1994)
   PUBMED   7984242
REFERENCE   9  (bases 1 to 4032)
  AUTHORS   Takagaki,Y. and Manley,J.L.
  TITLE     A human polyadenylation factor is a G protein beta-subunit
            homologue
  JOURNAL   J Biol Chem 267 (33), 23471-23474 (1992)
   PUBMED   1358884
REFERENCE   10 (bases 1 to 4032)
  AUTHORS   Takagaki,Y., MacDonald,C.C., Shenk,T. and Manley,J.L.
  TITLE     The human 64-kDa polyadenylylation factor contains a
            ribonucleoprotein-type RNA binding domain and unusual auxiliary
            motifs
  JOURNAL   Proc Natl Acad Sci U S A 89 (4), 1403-1407 (1992)
   PUBMED   1741396
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU189971.1, L02547.1,
            BE958524.1, BU189431.1 and AL121914.31.
            
            On May 17, 2019 this sequence version replaced NM_001324.2.
            
            Summary: This gene encodes one of three subunits which combine to
            form cleavage stimulation factor (CSTF). CSTF is involved in the
            polyadenylation and 3'end cleavage of pre-mRNAs. Similar to
            mammalian G protein beta subunits, this protein contains
            transducin-like repeats. Several transcript variants with different
            5' UTR, but encoding the same protein, have been found for this
            gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.195462.1,
                                           SRR1803613.171534.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000217109.9/ ENSP00000217109.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-576               BU189971.1         94-669
            577-1721            L02547.1           657-1801
            1722-1726           BE958524.1         56-60
            1727-2196           BU189431.1         1-470
            2197-4032           AL121914.31        76533-78368
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.2-q13.31"
     gene            1..4032
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /note="cleavage stimulation factor subunit 1"
                     /db_xref="GeneID:1477"
                     /db_xref="HGNC:HGNC:2483"
                     /db_xref="MIM:600369"
     exon            1..69
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /inference="alignment:Splign:2.1.0"
     exon            70..270
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1397
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /note="CSTF 50 kDa subunit; CF-1 50 kDa subunit; cleavage
                     stimulation factor 50 kDa subunit; cleavage stimulation
                     factor, 3' pre-RNA, subunit 1, 50kD; cleavage stimulation
                     factor, 3' pre-RNA, subunit 1, 50kDa"
                     /codon_start=1
                     /product="cleavage stimulation factor subunit 1"
                     /protein_id="NP_001315.1"
                     /db_xref="CCDS:CCDS13452.1"
                     /db_xref="GeneID:1477"
                     /db_xref="HGNC:HGNC:2483"
                     /db_xref="MIM:600369"
                     /translation="MYRTKVGLKDRQQLYKLIISQLLYDGYISIANGLINEIKPQSVC
                     APSEQLLHLIKLGMENDDTAVQYAIGRSDTVAPGTGIDLEFDADVQTMSPEASEYETC
                     YVTSHKGPCRVATYSRDGQLIATGSADASIKILDTERMLAKSAMPIEVMMNETAQQNM
                     ENHPVIRTLYDHVDEVTCLAFHPTEQILASGSRDYTLKLFDYSKPSAKRAFKYIQEAE
                     MLRSISFHPSGDFILVGTQHPTLRLYDINTFQCFVSCNPQDQHTDAICSVNYNSSANM
                     YVTGSKDGCIKLWDGVSNRCITTFEKAHDGAEVCSAIFSKNSKYILSSGKDSVAKLWE
                     ISTGRTLVRYTGAGLSGRQVHRTQAVFNHTEDYVLLPDERTISLCCWDSRTAERRNLL
                     SLGHNNIVRCIVHSPTNPGFMTCSDDFRARFWYRRSTTD"
     exon            271..548
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /inference="alignment:Splign:2.1.0"
     exon            549..746
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /inference="alignment:Splign:2.1.0"
     exon            747..1137
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..4032
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1699..1704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /note="hexamer: ATTAAA"
     polyA_site      1719
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /note="major polyA site"
     regulatory      1906..1911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /note="hexamer: AATAAA"
     polyA_site      1930
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /note="major polyA site"
     regulatory      4012..4017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
                     /note="hexamer: AATAAA"
     polyA_site      4032
                     /gene="CSTF1"
                     /gene_synonym="CstF-50; CstFp50"
ORIGIN      
        1 agattgggca ggattcgcgc ctccattttt ccaggagaga gcgggatacc aagagaaccg
       61 gaccagctgc tggcagggaa actgtcttcc ttttctccaa gatgtacaga accaaagtgg
      121 gcttgaagga ccgccagcag ctctacaagc tgatcattag ccagctgcta tatgacggct
      181 acatcagcat cgccaatggc ctcatcaatg aaatcaagcc tcagtctgtg tgtgcaccct
      241 cggagcagct cctgcatctc atcaaactcg gaatggaaaa cgatgacacc gcagttcagt
      301 atgcaattgg tcgttcagat actgttgccc ctggcacagg gattgacctg gaatttgatg
      361 cagatgttca gactatgtcc ccagaggctt ctgagtacga aacatgctat gtcacatcac
      421 ataaaggacc atgccgtgta gctacctata gtagagatgg acagttaata gctactgggt
      481 ctgctgatgc ttcgataaag atacttgaca cagagaggat gttggccaaa agtgccatgc
      541 caatagaggt catgatgaat gagaccgcac aacaaaatat ggaaaaccac ccagtgattc
      601 gaactcttta tgaccatgtg gatgaagtca cgtgccttgc tttccaccca acagaacaga
      661 tcctggcttc tggttcaagg gattatactc ttaaattatt tgattattcc aaaccatcag
      721 caaaaagagc cttcaaatac attcaggaag ctgaaatgtt acgttccatc tcttttcatc
      781 cttctggaga ctttatactt gtcggaactc agcatcctac tcttcgcctt tatgatatca
      841 acacctttca atgttttgtc tcttgcaatc ctcaagatca acacaccgat gctatatgtt
      901 ccgttaatta caattctagt gccaatatgt acgtaactgg aagcaaggac ggctgcatca
      961 aattatggga tggtgtttca aatcgatgca tcacaacttt tgagaaagca catgacggtg
     1021 ctgaagtttg ttctgccatt ttttccaaaa attctaaata cattctctca agtggaaaag
     1081 actctgtagc taaactttgg gaaatatcaa cgggacgaac actggtcaga tacacgggcg
     1141 cgggtttaag tggacgccag gtgcaccgga cacaggctgt gtttaaccac accgaggact
     1201 atgtgttgct gcccgacgag aggacgatca gtctttgctg ctgggactcg aggacagccg
     1261 agcggagaaa cctgctgtcg ttggggcaca acaatattgt acgctgcata gtgcactccc
     1321 ccaccaaccc cgggttcatg acgtgcagcg atgacttcag agcgcggttt tggtaccgga
     1381 gatcgaccac tgactgagcc accctctccg tagggttctt tctcgaggac tctaccctcc
     1441 tcccccacgt cctgtctcag ctgcagtcgt aagtccgtgc accatccttg acgttttgct
     1501 gccacctctg tccacattct tcttggattt gtataaaaga atcttttttt accttgatgt
     1561 agaatcatgg tggaaaaagt tggaaacaca gatctgtgca gttctacatt cactgattat
     1621 tacagtgtga ttttcatcgg ttttgtaagt acaggacttg ccgtttcttt tgatctcttg
     1681 attgaaggag gatagggcat taaagtgctt ttgacatgag gaattttcat ttggttcttc
     1741 tgtcaacttt cttgcttatt ctgctgaacc ctctgtgtat ttcccttcca ggtatatttg
     1801 aacagataag caggcatgca gctctgagac tcctgagttt tatgccatta tcaaaagcat
     1861 tttcaagaat ccctcaattc tatcctgaaa tgatgttatt ctgataataa agctcagatc
     1921 tgcaacttta ttgttatata tttttcactt ctgtaaaatt aatggccagt ttttcctgac
     1981 aatttatagg aacagtatct ttcaacatac tccctacatt atccttcaat atctgcaata
     2041 cttgtcttta gtaactgtat ttctggtaag attatgattt catgagaaac aattgaaaat
     2101 atatccgatc taattaaatt aggtcagcct gctattgctc tcaggtgttg cctgtcttca
     2161 cgaatgatta gtatcgattg acctctttct taggtggttt taagtcttca gttctcgtca
     2221 gcatgaacta acttctcatc atggaggcac atagtaactg cattctcaga agatttatgc
     2281 agttaaccaa ttgatacaag ttttcttttt cttgaatttt ttttttttta attgaggcac
     2341 agtcttgctc tgtcacccag gttggagtgc agtggcgtga tctcagctca ctgcaagctc
     2401 cgcctcctgg tttcacacca ttttcctgcc tcagcctccc aagtagctgg gactacaggc
     2461 acccaccacc acgcccggct aatttttttt tttttttttt ttttttttgt atttttagta
     2521 gagccagggt ttcaccatgt tagccaggat ggtctctatc tcctgacctc gtgatccgcc
     2581 tgcctcagcc tcccaaagtt ttgggattac aggcgtgagc caccgcgccc agccttcctg
     2641 aagcttttta gaacatgaca gtacttctct ggattcagca ctagcagagt cagagaactc
     2701 tgaactcccc ttgtacaaac ataaactttg ctttatattt atcccaaaaa acaaatacct
     2761 tatgatctgc ataggagtac ctagaaatat aatctgggat catttgataa aaatagtaaa
     2821 tagattgaag taaaagtcat tgctttatat tataggaagt tttgttttgt tttgttttgt
     2881 ttgttttgtt tttgagacgg agtttcgctc ttgtccccca ggctttagtg caatggcgcg
     2941 atctcggctc accgcagcct ccgccacctg ggttcaaacg attctcctgc ctcaggctcc
     3001 cgagtaacta ggattacagg cgtgcgccac catgcccggc taattttgta ttttttttag
     3061 tagagacggg gtttctccat gttggtcagg ctggtctcga actcccaacc tcaggtaatc
     3121 cgcccgcctc agcctcccaa agtgctagga ttacaggcgt gagccaccac gcgcggcccc
     3181 atcatacgaa gttactgaag ttattgcgta aacattctgt tttagtcatc cttgaggatc
     3241 gttttaactc cttgatctgt aaaactgtct taagcatctt aacatgttac atcacagggt
     3301 tttagctcag atttccaaag agccatattt tttgatgcat atttccagtt gaattccagt
     3361 gtgaatttcc agttgtactt tgcattctga ttctttgata tttgaacttg gccatctgtg
     3421 ggtgctactc tcgtaagact aaaaggcacc tactaaatac gaacaaggat aaccagttct
     3481 accaaaaaca ttaccaacca aacctcccct gttctttttt cagactttcc ttttgctttc
     3541 aacctcttaa actgtaaagg agagatttaa tgtttcccta ttattctgct ttgactcaca
     3601 tactaaaatg accacatggc actccattga atcttttcag ttgttcacga attaaaataa
     3661 tgcccatgtt ttacagtttt tcgagtagtc tcagcaatgg atctgtagtt tcaggacctg
     3721 tttttaaaag atcaagatac ttttatgtgt ttcagaagta gctgtttttt cattttttgt
     3781 gggtttgttt tttttgtgtt tgtttggttg gttggttttt ggtttttttg ctttttaaag
     3841 gtcacagtgt tgtgagcctg gtgtaaatgc caggaatatt atatggactt agtatctgca
     3901 tctcgttaat tgtaccacac aacactctgc cattcaaata atgaatatct ggtcaggata
     3961 cgaacttata ccctctgtgt gttttttttt tttaagatac gtgatttctg aaataaactt
     4021 tatatacaat ga
//
