LOCUS       NM_003650                891 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens cystatin F (CST7), mRNA.
ACCESSION   NM_003650
VERSION     NM_003650.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Senjor,E., Perisic Nanut,M., Breznik,B., Mitrovic,A., Mlakar,J.,
            Rotter,A., Porcnik,A., Lah Turnsek,T. and Kos,J.
  TITLE     Cystatin F acts as a mediator of immune suppression in glioblastoma
  JOURNAL   Cell Oncol (Dordr) 44 (5), 1051-1063 (2021)
   PUBMED   34189679
  REMARK    GeneRIF: Cystatin F acts as a mediator of immune suppression in
            glioblastoma.
REFERENCE   2  (bases 1 to 891)
  AUTHORS   Li,J., Durose,W.W., Ito,J., Kakita,A., Iguchi,Y., Katsuno,M.,
            Kunisawa,K., Shimizu,T. and Ikenaka,K.
  TITLE     Exploring the factors underlying remyelination arrest by studying
            the post-transcriptional regulatory mechanisms of cystatin F gene
  JOURNAL   J Neurochem 157 (6), 2070-2090 (2021)
   PUBMED   32947653
  REMARK    GeneRIF: Exploring the factors underlying remyelination arrest by
            studying the post-transcriptional regulatory mechanisms of cystatin
            F gene.
REFERENCE   3  (bases 1 to 891)
  AUTHORS   Sawyer,A.J., Garand,M., Chaussabel,D. and Feng,C.G.
  TITLE     Transcriptomic Profiling Identifies Neutrophil-Specific
            Upregulation of Cystatin F as a Marker of Acute Inflammation in
            Humans
  JOURNAL   Front Immunol 12, 634119 (2021)
   PUBMED   33868254
  REMARK    GeneRIF: Transcriptomic Profiling Identifies Neutrophil-Specific
            Upregulation of Cystatin F as a Marker of Acute Inflammation in
            Humans.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 891)
  AUTHORS   Hamilton,G., Colbert,J.D., Schuettelkopf,A.W. and Watts,C.
  TITLE     Cystatin F is a cathepsin C-directed protease inhibitor regulated
            by proteolysis
  JOURNAL   EMBO J 27 (3), 499-508 (2008)
   PUBMED   18256700
  REMARK    GeneRIF: A latent protease inhibitor which is itself regulated by
            proteolysis in the endocytic pathway.
REFERENCE   5  (bases 1 to 891)
  AUTHORS   Cappello,F., Gatti,E., Camossetto,V., David,A., Lelouard,H. and
            Pierre,P.
  TITLE     Cystatin F is secreted, but artificial modification of its
            C-terminus can induce its endocytic targeting
  JOURNAL   Exp Cell Res 297 (2), 607-618 (2004)
   PUBMED   15212960
  REMARK    GeneRIF: CST7 is secreted, but artificial modification of its
            C-terminus can induce its endocytic targeting.
REFERENCE   6  (bases 1 to 891)
  AUTHORS   Morita,M., Hara,Y., Tamai,Y., Arakawa,H. and Nishimura,S.
  TITLE     Genomic construct and mapping of the gene for CMAP
            (leukocystatin/cystatin F, CST7) and identification of a proximal
            novel gene, BSCv (C20orf3)
  JOURNAL   Genomics 67 (1), 87-91 (2000)
   PUBMED   10945474
REFERENCE   7  (bases 1 to 891)
  AUTHORS   Ni,J., Fernandez,M.A., Danielsson,L., Chillakuru,R.A., Zhang,J.,
            Grubb,A., Su,J., Gentz,R. and Abrahamson,M.
  TITLE     Cystatin F is a glycosylated human low molecular weight cysteine
            proteinase inhibitor
  JOURNAL   J Biol Chem 273 (38), 24797-24804 (1998)
   PUBMED   9733783
REFERENCE   8  (bases 1 to 891)
  AUTHORS   Brown,W.M. and Dziegielewska,K.M.
  TITLE     Friends and relations of the cystatin superfamily--new members and
            their evolution
  JOURNAL   Protein Sci 6 (1), 5-12 (1997)
   PUBMED   9007972
  REMARK    Review article
REFERENCE   9  (bases 1 to 891)
  AUTHORS   Thiesse,M., Millar,S.J. and Dickinson,D.P.
  TITLE     The human type 2 cystatin gene family consists of eight to nine
            members, with at least seven genes clustered at a single locus on
            human chromosome 20
  JOURNAL   DNA Cell Biol 13 (2), 97-116 (1994)
   PUBMED   8179826
REFERENCE   10 (bases 1 to 891)
  AUTHORS   Saitoh,E., Isemura,S., Sanada,K., Kim,H.S., Smithies,O. and
            Maeda,N.
  TITLE     Cystatin superfamily. Evidence that family II cystatin genes are
            evolutionarily related to family III cystatin genes
  JOURNAL   Biol Chem Hoppe Seyler 369 Suppl, 191-197 (1988)
   PUBMED   3202964
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG547285.1, AF031824.1 and
            CA449286.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003650.3.
            
            Summary: The cystatin superfamily encompasses proteins that contain
            multiple cystatin-like sequences. Some of the members are active
            cysteine protease inhibitors, while others have lost or perhaps
            never acquired this inhibitory activity. There are three inhibitory
            families in the superfamily, including the type 1 cystatins
            (stefins), type 2 cystatins and the kininogens. The type 2 cystatin
            proteins are a class of cysteine proteinase inhibitors found in a
            variety of human fluids and secretions. This gene encodes a
            glycosylated cysteine protease inhibitor with a putative role in
            immune regulation through inhibition of a unique target in the
            hematopoietic system. Expression of the protein has been observed
            in various human cancer cell lines established from malignant
            tumors. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM917046.1, SRR5189655.149795.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: experimental evidence
                                                (PMID:9733783)
            MANE Ensembl match               :: ENST00000480798.2/
                                                ENSP00000420384.1
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BG547285.1         41-62
            23-848              AF031824.1         1-826
            849-891             CA449286.1         11-53               c
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p11.21"
     gene            1..891
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /note="cystatin F"
                     /db_xref="GeneID:8530"
                     /db_xref="HGNC:HGNC:2479"
                     /db_xref="MIM:603253"
     exon            1..307
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..675
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /note="cystatin-like metastasis-associated protein;
                     cystatin-7; leukocystatin"
                     /codon_start=1
                     /product="cystatin-F precursor"
                     /protein_id="NP_003641.3"
                     /db_xref="CCDS:CCDS13165.2"
                     /db_xref="GeneID:8530"
                     /db_xref="HGNC:HGNC:2479"
                     /db_xref="MIM:603253"
                     /translation="MRAAGTLLAFCCLVLSTTGGPSPDTCSQDLNSRVKPGFPKTIKT
                     NDPGVLQAARYSVEKFNNCTNDMFLFKESRITRALVQIVKGLKYMLEVEIGRTTCKKN
                     QHLRLDDCDFQTNHTLKQTLSCYSEVWVVPWLQHFEVPVLRCH"
     exon            308..480
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            481..597
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /inference="alignment:Splign:2.1.0"
     exon            598..891
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      864..869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /note="hexamer: AATAGA"
     polyA_site      891
                     /gene="CST7"
                     /gene_synonym="CMAP"
                     /note="major polyA site"
ORIGIN      
        1 actgttccat gctgcccaag aaggctcagc acaggcacaa accattgccc ggcactggcc
       61 cgtgctgcct gagaaggatt ggcacgggca cagaccactg cccccacctg ccctgcgcca
      121 tctacccaag aaggctcggc acgggcacca accactgcct ccaactgccc catgctgcct
      181 gagaaggcac tgcacggcca cccccaactg ccccgcactg tccctacccg ggcagccatg
      241 cgagcggctg gaactctgct ggccttctgc tgcctggtct tgagcaccac tgggggccct
      301 tccccagata cttgttccca ggaccttaac tcacgtgtga agccaggatt tcctaaaaca
      361 ataaagacca atgacccagg agtcctccaa gcagccagat acagtgttga aaagttcaac
      421 aactgcacga acgacatgtt cttgttcaag gagtcccgca tcacaagggc cctagttcag
      481 atagtgaaag gcctgaaata tatgctggag gtggaaattg gcagaactac ctgcaagaaa
      541 aaccagcacc tgcgtctgga tgactgtgac ttccaaacca accacacctt gaagcagact
      601 ctgagctgct actctgaagt ctgggtcgtg ccctggctcc agcacttcga ggtgcctgtt
      661 ctccgttgtc actgaccccc gcctcttcag caagaccaca gccatgacaa acaccaggat
      721 gcatgctcct tgtcccctcc cacccgcctc atgacccagc ctcacagacc ctctcaggcc
      781 tctgacgagt gagcgggtga agtgccactg ggtcaccgca gggcagctgg aatggcagca
      841 tggtagcacc tcctaacaga ttaaatagat cacatttgct tctaaaatta a
//
