LOCUS       NM_130794                576 bp    mRNA    linear   PRI 17-APR-2022
DEFINITION  Homo sapiens cystatin 11 (CST11), transcript variant 1, mRNA.
ACCESSION   NM_130794
VERSION     NM_130794.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Frygelius J, Arvestad L, Wedell A and Tohonen V.
  TITLE     Evolution and human tissue expression of the Cres/Testatin subgroup
            genes, a reproductive tissue specific subgroup of the type 2
            cystatins
  JOURNAL   Evol. Dev. 12 (3), 329-342 (2010)
   PUBMED   20565543
REFERENCE   2  (bases 1 to 576)
  AUTHORS   Hamil KG, Liu Q, Sivashanmugam P, Yenugu S, Soundararajan R,
            Grossman G, Richardson RT, Zhang YL, O'Rand MG, Petrusz P, French
            FS and Hall SH.
  TITLE     Cystatin 11: a new member of the cystatin type 2 family
  JOURNAL   Endocrinology 143 (7), 2787-2796 (2002)
   PUBMED   12072414
  REMARK    GeneRIF: CST11 is an antimicrobial peptide that has shown to be
            effective against E. coli.
REFERENCE   3  (bases 1 to 576)
  AUTHORS   Brown WM and Dziegielewska KM.
  TITLE     Friends and relations of the cystatin superfamily--new members and
            their evolution
  JOURNAL   Protein Sci. 6 (1), 5-12 (1997)
   PUBMED   9007972
  REMARK    Review article
REFERENCE   4  (bases 1 to 576)
  AUTHORS   Dickinson DP, Zhao Y, Thiesse M and Siciliano MJ.
  TITLE     Direct mapping of seven genes encoding human type 2 cystatins to a
            single site located at 20p11.2
  JOURNAL   Genomics 24 (1), 172-175 (1994)
   PUBMED   7896273
REFERENCE   5  (bases 1 to 576)
  AUTHORS   Thiesse M, Millar SJ and Dickinson DP.
  TITLE     The human type 2 cystatin gene family consists of eight to nine
            members, with at least seven genes clustered at a single locus on
            human chromosome 20
  JOURNAL   DNA Cell Biol. 13 (2), 97-116 (1994)
   PUBMED   8179826
REFERENCE   6  (bases 1 to 576)
  AUTHORS   Dickinson DP, Thiesse M, Dempsey LD and Millar SJ.
  TITLE     Genomic cloning, physical mapping, and expression of human type 2
            cystatin genes
  JOURNAL   Crit. Rev. Oral Biol. Med. 4 (3-4), 573-580 (1993)
   PUBMED   7690606
REFERENCE   7  (bases 1 to 576)
  AUTHORS   Saitoh E, Isemura S, Sanada K, Kim HS, Smithies O and Maeda N.
  TITLE     Cystatin superfamily. Evidence that family II cystatin genes are
            evolutionarily related to family III cystatin genes
  JOURNAL   Biol. Chem. Hoppe-Seyler 369 Suppl, 191-197 (1988)
   PUBMED   3202964
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL096677.21 and AF335480.1.
            
            On Nov 22, 2018 this sequence version replaced NM_130794.1.
            
            Summary: The cystatin superfamily encompasses proteins that contain
            multiple cystatin-like sequences. Some of the members are active
            cysteine protease inhibitors, while others have lost or perhaps
            never acquired this inhibitory activity. There are three inhibitory
            families in the superfamily, including the type 1 cystatins
            (stefins), type 2 cystatins and the kininogens. The type 2 cystatin
            proteins are a class of cysteine proteinase inhibitors found in a
            variety of human fluids and secretions. The cystatin locus on
            chromosome 20 contains the majority of the type 2 cystatin genes
            and pseudogenes. This gene is located in the cystatin locus and
            encodes an epididymal-specific protein shown to have antimicrobial
            activity against E. coli. Alternative splicing yields two variants
            encoding distinct isoforms. [provided by RefSeq, Sep 2014].
            
            Transcript Variant: This variant (1) is the longest transcript,
            encoding the full-length isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF335480.1, BC121080.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA2142853,
                                           SAMEA2148874 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match                 :: ENST00000377009.8/
                                                  ENSP00000366208.3
            Protein has antimicrobial activity :: PMID: 12072414
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AL096677.21        108060-108090       c
            32-576              AF335480.1         1-545
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p11.21"
     gene            1..576
                     /gene="CST11"
                     /gene_synonym="CST8L; CTES2; dJ322G13.6; SC13"
                     /note="cystatin 11"
                     /db_xref="GeneID:140880"
                     /db_xref="HGNC:HGNC:15959"
                     /db_xref="MIM:609731"
     exon            1..293
                     /gene="CST11"
                     /gene_synonym="CST8L; CTES2; dJ322G13.6; SC13"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..482
                     /gene="CST11"
                     /gene_synonym="CST8L; CTES2; dJ322G13.6; SC13"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; cystatin-related epididymal-specific protein;
                     SC13delta; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="cystatin-11 isoform 1 precursor"
                     /protein_id="NP_570612.1"
                     /db_xref="CCDS:CCDS13155.1"
                     /db_xref="GeneID:140880"
                     /db_xref="HGNC:HGNC:15959"
                     /db_xref="MIM:609731"
                     /translation="MMAEPWQALQLLLAILLTLMALPYQARKKTFLSVHEVMAVENYA
                     KDSLQWITDQYNKESDDKYHFRIFRVLKVQRQVTDHLEYHLNVEMQWTTCQKPETTNC
                     VPQERELHKQVNCFFSVFAVPWFEQYKILNKSCSSD"
     exon            294..398
                     /gene="CST11"
                     /gene_synonym="CST8L; CTES2; dJ322G13.6; SC13"
                     /inference="alignment:Splign:2.1.0"
     exon            399..576
                     /gene="CST11"
                     /gene_synonym="CST8L; CTES2; dJ322G13.6; SC13"
                     /inference="alignment:Splign:2.1.0"
     regulatory      557..562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CST11"
                     /gene_synonym="CST8L; CTES2; dJ322G13.6; SC13"
                     /note="hexamer: AATAAA"
     polyA_site      576
                     /gene="CST11"
                     /gene_synonym="CST8L; CTES2; dJ322G13.6; SC13"
                     /note="major polyA site"
ORIGIN      
        1 agtgtgattc gacttgtccc tgaggagtac agagaacact cttcctgttc ctctgcagct
       61 gaaggatgat ggctgagccc tggcaggccc tacagctcct gttggccatt ctattgactc
      121 tgatggccct cccctaccaa gcaaggaaga aaacctttct aagcgtccat gaagtgatgg
      181 cagtagaaaa ctatgcgaag gacagcttgc agtggatcac cgaccagtat aacaaggaaa
      241 gtgatgacaa gtaccacttc aggatcttcc gagtccttaa agtccagagg caggtcactg
      301 accacctgga gtatcacctg aatgtggaaa tgcagtggac cacctgccaa aagccagaga
      361 ccacgaactg tgtcccccag gaaagggagc ttcacaagca agtcaactgc ttcttctcag
      421 tgtttgctgt accctggttt gaacagtaca aaattttgaa caaaagctgc agcagtgact
      481 aggtgtcctg gagtgtacaa ctgcggccac tgcatgtgag agaatcctgc aatgggcaat
      541 aatccttaat ataggcaata aatgtttgtc aacata
//
