LOCUS       NM_001321                908 bp    mRNA    linear   PRI 27-APR-2025
DEFINITION  Homo sapiens cysteine and glycine rich protein 2 (CSRP2),
            transcript variant 1, mRNA.
ACCESSION   NM_001321
VERSION     NM_001321.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Hayashi,K., Labios,R.J., Morita,T., Ashimori,A., Aoki,R., Mikuni,M.
            and Kimura,K.
  TITLE     Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic
            transition
  JOURNAL   Cell Struct Funct 48 (2), 199-210 (2023)
   PUBMED   37899269
  REMARK    GeneRIF: Significance of the p38MAPK-CRP2 axis in myofibroblastic
            phenotypic transition.
REFERENCE   2  (bases 1 to 908)
  AUTHORS   Hayashi,K., Horoiwa,S., Mori,K., Miyata,H., Labios,R.J., Morita,T.,
            Kobayashi,Y., Yamashiro,C., Higashijima,F., Yoshimoto,T., Kimura,K.
            and Nakagawa,Y.
  TITLE     Role of CRP2-MRTF interaction in functions of myofibroblasts
  JOURNAL   Cell Struct Funct 48 (1), 83-98 (2023)
   PUBMED   37164693
  REMARK    GeneRIF: Role of CRP2-MRTF interaction in functions of
            myofibroblasts.
REFERENCE   3  (bases 1 to 908)
  AUTHORS   Chen,L., Long,X., Duan,S., Liu,X., Chen,J., Lan,J., Liu,X.,
            Huang,W., Geng,J. and Zhou,J.
  TITLE     CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1
            axis-meditated ERK, PAK, and HIPPO signaling pathways
  JOURNAL   Theranostics 10 (24), 11063-11079 (2020)
   PUBMED   33042270
  REMARK    GeneRIF: CSRP2 suppresses colorectal cancer progression via
            p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 908)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 908)
  AUTHORS   Hoffmann,C., Mao,X., Brown-Clay,J., Moreau,F., Al Absi,A.,
            Wurzer,H., Sousa,B., Schmitt,F., Berchem,G., Janji,B. and Thomas,C.
  TITLE     Hypoxia promotes breast cancer cell invasion through
            HIF-1alpha-mediated up-regulation of the invadopodial actin
            bundling protein CSRP2
  JOURNAL   Sci Rep 8 (1), 10191 (2018)
   PUBMED   29976963
  REMARK    GeneRIF: Study showed that CSRP2, an invadopodial actin bundling
            protein, is upregulated by hypoxia in various breast cancer cell
            lines, as well as in pre-clinical and clinical breast tumor
            specimens and functionally characterized two hypoxia responsive
            elements within the proximal promoter of CSRP2 gene which are
            targeted by hypoxia-inducible factor-1 (HIF-1) and required for
            promoter transactivation in response to hypoxia.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 908)
  AUTHORS   Weiskirchen,R. and Gressner,A.M.
  TITLE     The cysteine- and glycine-rich LIM domain protein CRP2 specifically
            interacts with a novel human protein (CRP2BP)
  JOURNAL   Biochem Biophys Res Commun 274 (3), 655-663 (2000)
   PUBMED   10924333
REFERENCE   7  (bases 1 to 908)
  AUTHORS   Weiskirchen,R., Erdel,M., Utermann,G. and Bister,K.
  TITLE     Cloning, structural analysis, and chromosomal localization of the
            human CSRP2 gene encoding the LIM domain protein CRP2
  JOURNAL   Genomics 44 (1), 83-93 (1997)
   PUBMED   9286703
REFERENCE   8  (bases 1 to 908)
  AUTHORS   Jain,M.K., Fujita,K.P., Hsieh,C.M., Endege,W.O., Sibinga,N.E.,
            Yet,S.F., Kashiki,S., Lee,W.S., Perrella,M.A., Haber,E. and
            Lee,M.E.
  TITLE     Molecular cloning and characterization of SmLIM, a developmentally
            regulated LIM protein preferentially expressed in aortic smooth
            muscle cells
  JOURNAL   J Biol Chem 271 (17), 10194-10199 (1996)
   PUBMED   8626582
REFERENCE   9  (bases 1 to 908)
  AUTHORS   Karim,M.A., Ohta,K., Egashira,M., Jinno,Y., Niikawa,N., Matsuda,I.
            and Indo,Y.
  TITLE     Human ESP1/CRP2, a member of the LIM domain protein family:
            characterization of the cDNA and assignment of the gene locus to
            chromosome 14q32.3
  JOURNAL   Genomics 31 (2), 167-176 (1996)
   PUBMED   8824798
REFERENCE   10 (bases 1 to 908)
  AUTHORS   Weiskirchen,R., Pino,J.D., Macalma,T., Bister,K. and Beckerle,M.C.
  TITLE     The cysteine-rich protein family of highly related LIM domain
            proteins
  JOURNAL   J Biol Chem 270 (48), 28946-28954 (1995)
   PUBMED   7499425
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX461295.2, BC000992.2 and
            AC124784.4.
            
            On Nov 23, 2018 this sequence version replaced NM_001321.2.
            
            Summary: CSRP2 is a member of the CSRP family of genes, encoding a
            group of LIM domain proteins, which may be involved in regulatory
            processes important for development and cellular differentiation.
            CRP2 contains two copies of the cysteine-rich amino acid sequence
            motif (LIM) with putative zinc-binding activity, and may be
            involved in regulating ordered cell growth. Other genes in the
            family include CSRP1 and CSRP3. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes isoform b. Variants
            1-5 all encode the same isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.172419.1,
                                           SRR5189652.173068.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311083.10/ ENSP00000310901.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                BX461295.2         44-74
            32-902              BC000992.2         1-871
            903-908             AC124784.4         122895-122900
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.2"
     gene            1..908
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /note="cysteine and glycine rich protein 2"
                     /db_xref="GeneID:1466"
                     /db_xref="HGNC:HGNC:2470"
                     /db_xref="MIM:601871"
     exon            1..82
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /inference="alignment:Splign:2.1.0"
     exon            83..195
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..665
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /note="isoform b is encoded by transcript variant 1; LIM
                     domain only 5, smooth muscle; LMO-5; cysteine-rich protein
                     2; LIM domain only protein 5; smooth muscle cell LIM
                     protein; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="cysteine and glycine-rich protein 2 isoform b"
                     /protein_id="NP_001312.1"
                     /db_xref="CCDS:CCDS9015.1"
                     /db_xref="GeneID:1466"
                     /db_xref="HGNC:HGNC:2470"
                     /db_xref="MIM:601871"
                     /translation="MPVWGGGNKCGACGRTVYHAEEVQCDGRSFHRCCFLCMVCRKNL
                     DSTTVAIHDEEIYCKSCYGKKYGPKGYGYGQGAGTLNMDRGERLGIKPESVQPHRPTT
                     NPNTSKFAQKYGGAEKCSRCGDSVYAAEKIIGAGKPWHKNCFRCAKCGKSLESTTLTE
                     KEGEIYCKGCYAKNFGPKGFGYGQGAGALVHAQ"
     exon            196..364
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /inference="alignment:Splign:2.1.0"
     exon            365..494
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /inference="alignment:Splign:2.1.0"
     exon            495..588
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /inference="alignment:Splign:2.1.0"
     exon            589..908
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      885..890
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /note="hexamer: AATAAA"
     polyA_site      908
                     /gene="CSRP2"
                     /gene_synonym="CRP2; LMO5; SmLIM"
                     /note="major polyA site"
ORIGIN      
        1 gggatctcgg actccctgga ccctccctcc agcccagcct cgctagctcc gcctgcggta
       61 cgtgctcccg cctccgactc aaaatgcctg tctggggagg tggaaacaag tgtggggcct
      121 gtgggaggac cgtgtaccac gcagaagagg tgcagtgtga tggcaggagc ttccaccgct
      181 gctgctttct ctgcatggtt tgcaggaaaa atttagatag cacaacagtg gcaattcacg
      241 atgaagagat ctactgcaaa tcctgctacg gaaagaagta tgggccaaaa ggctacggtt
      301 atggccaggg cgctggcacg cttaacatgg accgtggcga gaggctgggc atcaaaccag
      361 agagtgttca gcctcacagg cctacaacaa atccaaacac ttctaaattt gctcagaaat
      421 atggaggtgc tgagaagtgt tccagatgtg gggattctgt atatgctgcc gagaagataa
      481 ttggagctgg aaagccctgg cacaaaaact gtttccgatg tgcaaagtgt gggaagagtc
      541 ttgaatcaac aactctgact gaaaaagaag gtgaaatcta ttgtaaagga tgctatgcaa
      601 agaactttgg gcccaaggga tttggctatg gccaaggagc aggggctctt gttcatgccc
      661 agtaagatgt aaaccctgaa ctaaacatca cacactgaga atctcttcat aatctaggca
      721 cagataatct ttaacactaa actactgtga aattctacca gcattaagta ctgtatatcg
      781 ccctgtactt ggataggctg gctaactcgt aggaagagag cactgtatgg tatccttttg
      841 ctttattcac cagcattttg ggggaacatt tcttttacat tttaaataaa acttcagctt
      901 gatttggg
//
