LOCUS       NM_001382391            4859 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens centrosome and spindle pole associated protein 1
            (CSPP1), transcript variant 8, mRNA.
ACCESSION   NM_001382391 XM_024447278
VERSION     NM_001382391.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   van den Berg,C.M., Volkov,V.A., Schnorrenberg,S., Huang,Z.,
            Stecker,K.E., Grigoriev,I., Gilani,S., Frikstad,K.M., Patzke,S.,
            Zimmermann,T., Dogterom,M. and Akhmanova,A.
  TITLE     CSPP1 stabilizes growing microtubule ends and damaged lattices from
            the luminal side
  JOURNAL   J Cell Biol 222 (4) (2023)
   PUBMED   36752787
  REMARK    GeneRIF: CSPP1 stabilizes growing microtubule ends and damaged
            lattices from the luminal side.
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Odabasi,E., Conkar,D., Deretic,J., Batman,U., Frikstad,K.M.,
            Patzke,S. and Firat-Karalar,E.N.
  TITLE     CCDC66 regulates primary cilium length and signaling via
            interactions with transition zone and axonemal proteins
  JOURNAL   J Cell Sci 136 (3) (2023)
   PUBMED   36606424
REFERENCE   3  (bases 1 to 4859)
  AUTHORS   Zhang,L., Yang,S.T., Wang,C., Zhang,L.C., Zhang,X., Li,F.C.,
            Wang,S.Y. and Ma,K.
  TITLE     Circle RNA circCSPP1 promotes human osteosarcoma cell proliferation
            and increases glucose metabolism by suppressing miR-200c maturation
  JOURNAL   Hum Exp Toxicol 41, 9603271221097364 (2022)
   PUBMED   35713481
  REMARK    GeneRIF: Circle RNA circCSPP1 promotes human osteosarcoma cell
            proliferation and increases glucose metabolism by suppressing
            miR-200c maturation.
REFERENCE   4  (bases 1 to 4859)
  AUTHORS   Latour,B.L., Van De Weghe,J.C., Rusterholz,T.D., Letteboer,S.J.,
            Gomez,A., Shaheen,R., Gesemann,M., Karamzade,A., Asadollahi,M.,
            Barroso-Gil,M., Chitre,M., Grout,M.E., van Reeuwijk,J., van
            Beersum,S.E., Miller,C.V., Dempsey,J.C., Morsy,H., Bamshad,M.J.,
            Nickerson,D.A., Neuhauss,S.C., Boldt,K., Ueffing,M.,
            Keramatipour,M., Sayer,J.A., Alkuraya,F.S., Bachmann-Gagescu,R.,
            Roepman,R. and Doherty,D.
  CONSRTM   University of Washington Center for Mendelian Genomics; Genomics
            England Research Consortium
  TITLE     Dysfunction of the ciliary ARMC9/TOGARAM1 protein module causes
            Joubert syndrome
  JOURNAL   J Clin Invest 130 (8), 4423-4439 (2020)
   PUBMED   32453716
REFERENCE   5  (bases 1 to 4859)
  AUTHORS   Xue,Y.F., Li,M., Li,W., Lin,Q., Yu,B.X., Zhu,Q.B. and Chen,H.J.
  TITLE     Roles of circ-CSPP1 on the proliferation and metastasis of glioma
            cancer
  JOURNAL   Eur Rev Med Pharmacol Sci 24 (10), 5519-5525 (2020)
   PUBMED   32495924
  REMARK    GeneRIF: Roles of circ-CSPP1 on the proliferation and metastasis of
            glioma cancer.
REFERENCE   6  (bases 1 to 4859)
  AUTHORS   Patzke,S., Redick,S., Warsame,A., Murga-Zamalloa,C.A., Khanna,H.,
            Doxsey,S. and Stokke,T.
  TITLE     CSPP is a ciliary protein interacting with Nephrocystin 8 and
            required for cilia formation
  JOURNAL   Mol Biol Cell 21 (15), 2555-2567 (2010)
   PUBMED   20519441
  REMARK    GeneRIF: CSPP isoforms require their common C-terminal domain to
            interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary
            complex with NPHP8 and NPHP4.
REFERENCE   7  (bases 1 to 4859)
  AUTHORS   Asiedu,M., Wu,D., Matsumura,F. and Wei,Q.
  TITLE     Centrosome/spindle pole-associated protein regulates cytokinesis
            via promoting the recruitment of MyoGEF to the central spindle
  JOURNAL   Mol Biol Cell 20 (5), 1428-1440 (2009)
   PUBMED   19129481
  REMARK    GeneRIF: CSPP interacts with and recruits MyoGEF to the central
            spindle, where MyoGEF contributes to the spatiotemporal regulation
            of cytokinesis.
REFERENCE   8  (bases 1 to 4859)
  AUTHORS   Patzke,S., Stokke,T. and Aasheim,H.C.
  TITLE     CSPP and CSPP-L associate with centrosomes and microtubules and
            differently affect microtubule organization
  JOURNAL   J Cell Physiol 209 (1), 199-210 (2006)
   PUBMED   16826565
  REMARK    GeneRIF: Taken together, CSPP and CSPP-L interact with centrosomes
            and microtubules and can differently affect microtubule
            organization.
REFERENCE   9  (bases 1 to 4859)
  AUTHORS   Patzke,S., Hauge,H., Sioud,M., Finne,E.F., Sivertsen,E.A.,
            Delabie,J., Stokke,T. and Aasheim,H.C.
  TITLE     Identification of a novel centrosome/microtubule-associated
            coiled-coil protein involved in cell-cycle progression and spindle
            organization
  JOURNAL   Oncogene 24 (7), 1159-1173 (2005)
   PUBMED   15580290
  REMARK    GeneRIF: Novel centrosome/microtubule-associated coiled-coil
            protein (CSPP)is associated with centrosomes and microtubules and
            may play a role in the regulation of G(1)/S-phase progression and
            spindle assembly [CSPP].
REFERENCE   10 (bases 1 to 4859)
  AUTHORS   Parisi,M. and Glass,I.
  TITLE     Joubert Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301500
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC109335.5 and AC087359.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 6, 2020 this sequence version replaced XM_024447278.1.
            
            Summary: This gene encodes a centrosome and spindle pole associated
            protein. The encoded protein plays a role in cell-cycle progression
            and spindle organization, regulates cytokinesis, interacts with
            Nephrocystin 8 and is required for cilia formation. Mutations in
            this gene result in primary cilia abnormalities and classical
            Joubert syndrome. Alternatively spliced transcript variants
            encoding distinct isoforms have been found for this gene. [provided
            by RefSeq, Apr 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968968,
                              SAMEA2142348 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000678616.1/ ENSP00000504733.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               AC109335.5         69739-69885         c
            148-256             AC109335.5         59926-60034         c
            257-356             AC109335.5         57696-57795         c
            357-460             AC109335.5         48167-48270         c
            461-541             AC109335.5         42394-42474         c
            542-640             AC109335.5         40636-40734         c
            641-1080            AC109335.5         38545-38984         c
            1081-1179           AC109335.5         31142-31240         c
            1180-1250           AC109335.5         28302-28372         c
            1251-1344           AC109335.5         22212-22305         c
            1345-1402           AC109335.5         20415-20472         c
            1403-1444           AC109335.5         19907-19948         c
            1445-1653           AC109335.5         18155-18363         c
            1654-1775           AC109335.5         15908-16029         c
            1776-1854           AC109335.5         15456-15534         c
            1855-1984           AC109335.5         2197-2326           c
            1985-2132           AC087359.13        151484-151631       c
            2133-2285           AC087359.13        139152-139304       c
            2286-2398           AC087359.13        134951-135063       c
            2399-2548           AC087359.13        130491-130640       c
            2549-2695           AC087359.13        129950-130096       c
            2696-2800           AC087359.13        127172-127276       c
            2801-2867           AC087359.13        125289-125355       c
            2868-2985           AC087359.13        124579-124696       c
            2986-3125           AC087359.13        116532-116671       c
            3126-3266           AC087359.13        113651-113791       c
            3267-3313           AC087359.13        111361-111407       c
            3314-3377           AC087359.13        109161-109224       c
            3378-3487           AC087359.13        98328-98437         c
            3488-3626           AC087359.13        95485-95623         c
            3627-4859           AC087359.13        92473-93705         c
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.1-q13.2"
     gene            1..4859
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /note="centrosome and spindle pole associated protein 1"
                     /db_xref="GeneID:79848"
                     /db_xref="HGNC:HGNC:26193"
                     /db_xref="MIM:611654"
     exon            1..147
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            148..256
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..3838
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /note="isoform 8 is encoded by transcript variant 8"
                     /codon_start=1
                     /product="centrosome and spindle pole-associated protein 1
                     isoform 8"
                     /protein_id="NP_001369320.1"
                     /db_xref="CCDS:CCDS94307.1"
                     /db_xref="GeneID:79848"
                     /db_xref="HGNC:HGNC:26193"
                     /db_xref="MIM:611654"
                     /translation="MADNLDEFIEEQKARLAEDKAELESDPPYMEMKGKLSAKLSENS
                     KILISMAKENIPPNSQQTRGSLGIDYGLSLPLGEDYERKKHKLKEELRQDYRRYLTQK
                     NFLSTSETDPSTLGVSLPIGERLSAKERLKLERNKEYNQFLRGKEESSEKFRQVEKST
                     EPKSQRNKKPIGQVKPDLTSQIQTSCENSEGPRKDVLTPSEAYEELLNQRRLEEDRYR
                     QLDDEIELRNRRIIKKANEEVGISNLKHQRFASKAGIPDRRFHRFNEDRVFDRRYHRP
                     DQDPEVSEEMDERFRYESDFDRRLSRVYTNDRMHRNKRGNMPPMEHDGDVIEQSNIRI
                     SSAENKSAPDNETSKSANQDTCSPFAGMLFGGEDRELIQRRKEKYRLELLEQMAEQQR
                     NKRREKDLELRVAASGAQDPEKSWNELLTSLQLSKEEPDRLKQFSVAPRHFEEMIPPE
                     RPRIAFQTPLPPLSAPSVPPIPSVHPVPSQNEDLRSGLSSALGEMVSPRIAPLPPPPL
                     LPPLATNYRTPYDDAYYFYGSRNTFDPSLAYYGSGMMGVQPAAYVSAPVTHQLAQPVV
                     NTVGQNELKITSDQVINSGLIFEDKPKPSKQSLQSYQEALQQQIREREERRKKEREEK
                     EEYEAKLEAEMRTYNPWGKGGGGAPLRDAKGNLITDLNRMHRQNIDAYHNPDARTYED
                     KRAVVSLDPNLATSNAENLEDAANKSSGHMQTQSSPFARGNVFGEPPTELQIKQQELY
                     KNFLRFQIEEKKQREEAERERLRIAEEKEERRLAEQRARIQQEYEEEQEKKREKEEEQ
                     RLKNEEHIRLAEERQKEAERKKKEEEEKYNLQLQHYCERDNLIGEETKHMRQPSPIVP
                     ALQNKIASKLQRPPSVDSIIRSFIHESSMSRAQSPPVPARKNQLRAEEEKKNVIMELS
                     EMRKQLRSEERRLQERLLHMDSDDEIPIRKKERNPMDIFDMARHRLQAPVRRQSPKGL
                     DAATFQNVHDFNELKDRDSETRVDLKFMYLDPPRDHHTLEIQQQALLREQQKRLNRIK
                     MQEGAKVDLDAIPSAKVREQRMPRDDTSDFLKNSLLESDSAFIGAYGETYPAIEDDVL
                     PPPSQLPSARERRRNKWKGLDIDSSRPNVAPDGLSLKSISSVNVDELRVRNEERMRRL
                     NEFHNKPINTDDESSLVDPDDIMKHIGDDGSNSVATEPWLRPGTSETLKRFMAEQLNQ
                     EQQQIPGKPGTFTWQGLSTAHG"
     exon            257..356
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            357..460
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            461..541
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            542..640
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            641..1080
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1179
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1250
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1344
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1402
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1444
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1653
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1775
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1854
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1984
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2132
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2285
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2398
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2399..2548
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2549..2695
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2696..2800
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2867
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..2985
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            2986..3125
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            3126..3266
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3313
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            3314..3377
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            3378..3487
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            3488..3626
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     exon            3627..4859
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4482..4487
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /note="hexamer: AATAAA"
     polyA_site      4508
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /note="major polyA site"
     regulatory      4839..4844
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /note="hexamer: ATTAAA"
     polyA_site      4859
                     /gene="CSPP1"
                     /gene_synonym="CSPP; CSPP-L; JBTS21"
                     /note="major polyA site"
ORIGIN      
        1 gtctgtcatg ctgttcccgc tccaggtggc cgctgtaacc tcttcggtcc gcgacgatcc
       61 tctagagcac tgtgtgtctc cccggacgcg agcccgctcc cctgagtaag agtcagccag
      121 ccgcggatgg ggagcgtgag tggcgagaat ctgcaaaatg gctgataatt tggatgaatt
      181 tattgaagag caaaaagcca gattggccga agacaaagca gagttggaaa gtgatccacc
      241 ttacatggaa atgaagggaa agttgtcagc gaagctttct gaaaacagta agatactgat
      301 ctctatggct aaggaaaaca taccaccaaa tagtcaacag accaggggtt ccttaggaat
      361 tgattatgga ttaagtttac cacttggaga agactatgaa cggaagaaac ataaattaaa
      421 agaagaattg cggcaagatt acagacgtta tcttactcag aaaaattttc tatctacgag
      481 tgaaacagat ccatctactt tgggagtttc tcttcctatt ggtgagaggt tatctgctaa
      541 ggaaaggttg aaacttgaac gtaacaaaga atacaatcag tttctcaggg gtaaggaaga
      601 atccagtgaa aagttcaggc aggtggaaaa gagtactgag cccaagagtc agagaaataa
      661 aaaacctatt ggtcaagtta agcctgatct aacttcacaa atacagacat cttgtgaaaa
      721 ttcagagggt cctagaaaag atgtcttaac tccttcagag gcatatgaag aacttctgaa
      781 ccaaagacga ctagaggagg acagataccg acaactagat gatgaaatcg aattaaggaa
      841 tagaagaatt attaaaaaag caaatgaaga agtgggcatt tccaacctaa aacatcaaag
      901 gtttgcaagc aaggctggca ttccagatag aagatttcac agatttaatg aggatcgtgt
      961 ttttgataga cggtatcata gaccagacca agatcctgaa gtaagtgaag aaatggatga
     1021 gaggtttaga tatgaaagtg attttgatag aagactttcg agagtgtata caaatgacag
     1081 gatgcacagg aacaaacgag ggaatatgcc tcctatggaa catgatgggg atgttataga
     1141 acagtcaaac ataagaattt catctgctga aaataaaagt gctccagaca atgaaacatc
     1201 caaatctgct aatcaagata cctgtagtcc ttttgcaggg atgctctttg gaggtgaaga
     1261 tcgagaactt attcagagaa ggaaagagaa atacagacta gaactgttgg aacaaatggc
     1321 tgagcaacag aggaacaaga gacgagaaaa agatttagaa ctcagggttg cagcgtctgg
     1381 agcacaagac cctgagaaat cgtggaatga attactcaca tcattgcagt taagtaagga
     1441 ggagcctgat agactaaagc agtttagtgt ggcaccaaga cactttgaag agatgatacc
     1501 acctgaaaga cccagaatag ctttccagac acctctccct cctttatctg ccccatctgt
     1561 cccacccatc ccatcagttc atcctgttcc ttctcaaaat gaagatttgc gcagtggact
     1621 cagcagcgcc cttggtgaaa tggtgtctcc caggattgca cctctgcctc cacctcccct
     1681 actaccacct ttggctacta actatcgaac tccttatgat gatgcatact atttttatgg
     1741 gtccaggaat actttcgatc ccagtcttgc ttattatggt tcaggaatga tgggcgtaca
     1801 gcctgcagct tatgttagtg ctcctgtcac ccaccaacta gcacaacctg ttgtgaatac
     1861 ggttggacag aatgaactga agattacaag tgatcaagtg ataaattcag gattgatttt
     1921 tgaagataaa ccgaaacctt ccaaacagtc acttcagtct taccaagagg ctttgcagca
     1981 gcagattcgg gaaagagaag aaagaaggaa gaaagaacgt gaagaaaaag aagaatatga
     2041 agctaaatta gaagctgaaa tgagaacata taatccctgg ggaaaaggtg gaggtggtgc
     2101 tcctctcagg gatgcaaaag gaaatctgat aactgatttg aataggatgc acagacaaaa
     2161 tatagatgcc taccataacc cagatgcaag aacatatgaa gataaaaggg ctgttgtatc
     2221 tctagaccca aatttagcca cttcaaatgc tgagaaccta gaagatgctg caaataaaag
     2281 ctcaggtcat atgcaaacac agagctctcc ttttgctcgg ggaaatgtat ttggtgagcc
     2341 tccaactgaa cttcagatta aacagcaaga attatacaag aattttcttc gtttccagat
     2401 tgaggaaaag aaacaaagag aggaagcaga gcgagagaga ctgagaattg cagaagaaaa
     2461 agaagaaaga cggcttgcag aacagagggc acgaattcag caggagtatg aagaggaaca
     2521 ggaaaagaaa agagagaaag aggaggagca aaggctaaaa aatgaagagc atattcggtt
     2581 agctgaagaa agacaaaaag aagcagaaag aaagaagaaa gaagaagaag aaaaatataa
     2641 cctgcaactt cagcactact gtgaaagaga caatttgatt ggggaagaaa caaagcacat
     2701 gagacagcct tctcctatag ttcctgctct tcagaacaaa attgcaagca aactccaaag
     2761 acctccttca gttgacagca tcatacgttc ctttattcat gaaagttcca tgtccagggc
     2821 acagtcaccc ccggtacctg ccaggaaaaa tcagctccgt gcagaagagg agaaaaaaaa
     2881 tgtaattatg gaattatcag aaatgagaaa acagcttcgt agtgaagaga ggcgtctaca
     2941 agagcgattg ctacacatgg acagtgatga tgaaattcct atcaggaaaa aggaaaggaa
     3001 tcccatggat atatttgata tggctagaca tcggttgcaa gctcctgtca gaagacagtc
     3061 ccctaagggc ttagacgctg ccacttttca gaatgttcat gattttaatg agctgaaaga
     3121 tagagattca gaaacacgag ttgatctgaa atttatgtac ctggatcctc caagagatca
     3181 tcacacctta gagattcagc agcaagccct gctaagagag cagcagaaga ggctgaacag
     3241 aataaaaatg caggaaggtg ccaaagttga cttagatgcc atcccaagtg ctaaagtacg
     3301 agagcaaaga atgcccagag atgacactag tgatttcttg aaaaactcat tattggaatc
     3361 tgatagtgct tttattgggg cttacggtga gacatatcct gccattgaag atgacgtcct
     3421 ccctccacca tcacagttgc cctctgcacg ggagcgcagg aggaacaaat ggaaaggact
     3481 agacattgat agcagtcgtc ctaatgtagc accagatggt ctctctctaa aatctatatc
     3541 cagtgtaaat gttgatgagc ttagagtgag aaatgaggaa cgaatgcgaa gactgaatga
     3601 atttcacaat aaacctatta atacagatga tgagagttca ctggttgacc ctgatgacat
     3661 catgaaacac ataggggatg acggatcaaa ctctgtagca actgagccct ggctccgccc
     3721 tggcacttca gaaacgctga aacgtttcat ggcagagcag ctgaaccagg agcagcagca
     3781 gattcctgga aaaccaggca ctttcacttg gcagggcctg tcgactgcac atggttaaaa
     3841 taaacctgta ctggacccag tagtgccttt taaggtgaaa ggaatggtaa atctgtacct
     3901 ttaatatgtc ctacttttgg cccctacctg aaagttactt tttttccatc atctgtatat
     3961 aaaattattt ttatcatgat gtatattatg tacataaata aaaggccatg attattgatt
     4021 tatataatag aattgtatag attatttttg cacagttttg tcataaatta gggtggtaat
     4081 gaactggatt gaactactat atgtgcatta tattgaattc tgcttgtcat taagataagg
     4141 tgaataagtg tcttaaacgt cctgtaaaac cggactcccc tttgttacat gcacattttc
     4201 cattgttacc tcgatgcaaa agaattcatt tagtaggtac atctattgta gctgtgatta
     4261 ttccagtttc tgtgtgatgc aatcaaatgt cctattaatt aattattatt tcatgtcatt
     4321 tgtagctact gatacagcag aaatgaaggg aactgtaatt acttgtattt ttgtaagcca
     4381 tacgttaaat gtttgttaca tcatctttct gcttctattt ttatgccaat gaaggcattt
     4441 gtcttgttac taattacatg atgtaactac ttcttgatat aaataaattt ttattttaat
     4501 tactaaaatc tttttaacta ctatggagct ttctagacta gttttctaga ggttgaatag
     4561 aggtggggac acccgggagt caaggacaga gagactggag tcttcttctt ccaccatgga
     4621 accttggata agtgagtcca tgtgagtttt ctaatcactc agtaagtgat acttctaaaa
     4681 aaggaaagag tcattcaaat aattgtgaca ttctgactca gattagaatc tcagaaccat
     4741 gacagctgag aacatcccca ggcactgctt ctctctctaa taatacttcc tctgatgtaa
     4801 ttaacggttg gtagacaata tcagacaaat ttgccacaat taaattactg atattttga
//
