LOCUS       NM_001897               8290 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.
ACCESSION   NM_001897
VERSION     NM_001897.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Uno,K., Koya,Y., Yoshihara,M., Iyoshi,S., Kitami,K., Sugiyama,M.,
            Miyamoto,E., Mogi,K., Fujimoto,H., Yamakita,Y., Wang,X., Nawa,A.
            and Kajiyama,H.
  TITLE     Chondroitin Sulfate Proteoglycan 4 Provides New Treatment Approach
            to Preventing Peritoneal Dissemination in Ovarian Cancer
  JOURNAL   Int J Mol Sci 25 (3), 1626 (2024)
   PUBMED   38338902
  REMARK    GeneRIF: Chondroitin Sulfate Proteoglycan 4 Provides New Treatment
            Approach to Preventing Peritoneal Dissemination in Ovarian Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Bagheri Varzaneh,M., Zhao,Y., Rozynek,J., Han,M. and Reed,D.A.
  TITLE     Disrupting mechanical homeostasis promotes matrix
            metalloproteinase-13 mediated processing of neuron glial antigen 2
            in mandibular condylar cartilage
  JOURNAL   Eur Cell Mater 45, 113-130 (2023)
   PUBMED   37154195
  REMARK    GeneRIF: Disrupting mechanical homeostasis promotes matrix
            metalloproteinase-13 mediated processing of neuron glial antigen 2
            in mandibular condylar cartilage.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8290)
  AUTHORS   Larabee,J.L., Doyle,D.A., Ahmed,U.K.B., Shadid,T.M., Sharp,R.R.,
            Jones,K.L., Kim,Y.M., Li,S. and Ballard,J.D.
  TITLE     Discovery of Hippo signaling as a regulator of CSPG4 expression and
            as a therapeutic target for Clostridioides difficile disease
  JOURNAL   PLoS Pathog 19 (3), e1011272 (2023)
   PUBMED   36972308
  REMARK    GeneRIF: Discovery of Hippo signaling as a regulator of CSPG4
            expression and as a therapeutic target for Clostridioides difficile
            disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8290)
  AUTHORS   Xu,J., Yuan,A., Su,R., Yang,Q., Fan,X. and Zhang,J.
  TITLE     Trophoblast-specific knockdown of CSPG4 expression causes pregnancy
            complications with poor placentation in mice
  JOURNAL   Reprod Biol 23 (1), 100731 (2023)
   PUBMED   36634519
  REMARK    GeneRIF: Trophoblast-specific knockdown of CSPG4 expression causes
            pregnancy complications with poor placentation in mice.
REFERENCE   5  (bases 1 to 8290)
  AUTHORS   Yang,L., Zhang,H., Dong,C., Yue,W., Xue,R., Liu,F., Yang,L. and
            Li,L.
  TITLE     Neuron-Glial Antigen 2 Participates in Liver Fibrosis via
            Regulating the Differentiation of Bone Marrow Mesenchymal Stem Cell
            to Myofibroblast
  JOURNAL   Int J Mol Sci 24 (2), 1177 (2023)
   PUBMED   36674693
  REMARK    GeneRIF: Neuron-Glial Antigen 2 Participates in Liver Fibrosis via
            Regulating the Differentiation of Bone Marrow Mesenchymal Stem Cell
            to Myofibroblast.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8290)
  AUTHORS   Barritt,D.S., Pearn,M.T., Zisch,A.H., Lee,S.S., Javier,R.T.,
            Pasquale,E.B. and Stallcup,W.B.
  TITLE     The multi-PDZ domain protein MUPP1 is a cytoplasmic ligand for the
            membrane-spanning proteoglycan NG2
  JOURNAL   J Cell Biochem 79 (2), 213-224 (2000)
   PUBMED   10967549
REFERENCE   7  (bases 1 to 8290)
  AUTHORS   Eisenmann,K.M., McCarthy,J.B., Simpson,M.A., Keely,P.J., Guan,J.L.,
            Tachibana,K., Lim,L., Manser,E., Furcht,L.T. and Iida,J.
  TITLE     Melanoma chondroitin sulphate proteoglycan regulates cell spreading
            through Cdc42, Ack-1 and p130cas
  JOURNAL   Nat Cell Biol 1 (8), 507-513 (1999)
   PUBMED   10587647
REFERENCE   8  (bases 1 to 8290)
  AUTHORS   Iida,J., Meijne,A.M., Oegema,T.R. Jr., Yednock,T.A., Kovach,N.L.,
            Furcht,L.T. and McCarthy,J.B.
  TITLE     A role of chondroitin sulfate glycosaminoglycan binding site in
            alpha4beta1 integrin-mediated melanoma cell adhesion
  JOURNAL   J Biol Chem 273 (10), 5955-5962 (1998)
   PUBMED   9488735
REFERENCE   9  (bases 1 to 8290)
  AUTHORS   Kirschfink,M., Blase,L., Engelmann,S. and Schwartz-Albiez,R.
  TITLE     Secreted chondroitin sulfate proteoglycan of human B cell lines
            binds to the complement protein C1q and inhibits complex formation
            of C1
  JOURNAL   J Immunol 158 (3), 1324-1331 (1997)
   PUBMED   9013976
REFERENCE   10 (bases 1 to 8290)
  AUTHORS   Pluschke,G., Vanek,M., Evans,A., Dittmar,T., Schmid,P., Itin,P.,
            Filardo,E.J. and Reisfeld,R.A.
  TITLE     Molecular cloning of a human melanoma-associated chondroitin
            sulfate proteoglycan
  JOURNAL   Proc Natl Acad Sci U S A 93 (18), 9710-9715 (1996)
   PUBMED   8790396
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP363063.1, AY359468.1,
            X96753.1, BQ679485.1, CA428796.1 and CA438367.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001897.4.
            
            Summary: A human melanoma-associated chondroitin sulfate
            proteoglycan plays a role in stabilizing cell-substratum
            interactions during early events of melanoma cell spreading on
            endothelial basement membranes. CSPG4 represents an integral
            membrane chondroitin sulfate proteoglycan expressed by human
            malignant melanoma cells. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X96753.1, AY359468.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162568 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308508.5/ ENSP00000312506.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-181               BP363063.1         1-181
            182-3512            AY359468.1         89-3419
            3513-3620           X96753.1           3420-3527
            3621-7104           AY359468.1         3528-7011
            7105-7766           BQ679485.1         67-728
            7767-7989           CA428796.1         18-240              c
            7990-8290           CA438367.1         16-316              c
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q24.2"
     gene            1..8290
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /note="chondroitin sulfate proteoglycan 4"
                     /db_xref="GeneID:1464"
                     /db_xref="HGNC:HGNC:2466"
                     /db_xref="MIM:601172"
     exon            1..181
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..7062
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /note="chondroitin sulfate proteoglycan 4
                     (melanoma-associated); melanoma-associated chondroitin
                     sulfate proteoglycan; chondroitin sulfate proteoglycan
                     NG2; melanoma chondroitin sulfate proteoglycan"
                     /codon_start=1
                     /product="chondroitin sulfate proteoglycan 4 precursor"
                     /protein_id="NP_001888.2"
                     /db_xref="CCDS:CCDS10284.1"
                     /db_xref="GeneID:1464"
                     /db_xref="HGNC:HGNC:2466"
                     /db_xref="MIM:601172"
                     /translation="MQSGPRPPLPAPGLALALTLTMLARLASAASFFGENHLEVPVAT
                     ALTDIDLQLQFSTSQPEALLLLAAGPADHLLLQLYSGRLQVRLVLGQEELRLQTPAET
                     LLSDSIPHTVVLTVVEGWATLSVDGFLNASSAVPGAPLEVPYGLFVGGTGTLGLPYLR
                     GTSRPLRGCLHAATLNGRSLLRPLTPDVHEGCAEEFSASDDVALGFSGPHSLAAFPAW
                     GTQDEGTLEFTLTTQSRQAPLAFQAGGRRGDFIYVDIFEGHLRAVVEKGQGTVLLHNS
                     VPVADGQPHEVSVHINAHRLEISVDQYPTHTSNRGVLSYLEPRGSLLLGGLDAEASRH
                     LQEHRLGLTPEATNASLLGCMEDLSVNGQRRGLREALLTRNMAAGCRLEEEEYEDDAY
                     GHYEAFSTLAPEAWPAMELPEPCVPEPGLPPVFANFTQLLTISPLVVAEGGTAWLEWR
                     HVQPTLDLMEAELRKSQVLFSVTRGARHGELELDIPGAQARKMFTLLDVVNRKARFIH
                     DGSEDTSDQLVLEVSVTARVPMPSCLRRGQTYLLPIQVNPVNDPPHIIFPHGSLMVIL
                     EHTQKPLGPEVFQAYDPDSACEGLTFQVLGTSSGLPVERRDQPGEPATEFSCRELEAG
                     SLVYVHRGGPAQDLTFRVSDGLQASPPATLKVVAIRPAIQIHRSTGLRLAQGSAMPIL
                     PANLSVETNAVGQDVSVLFRVTGALQFGELQKQGAGGVEGAEWWATQAFHQRDVEQGR
                     VRYLSTDPQHHAYDTVENLALEVQVGQEILSNLSFPVTIQRATVWMLRLEPLHTQNTQ
                     QETLTTAHLEATLEEAGPSPPTFHYEVVQAPRKGNLQLQGTRLSDGQGFTQDDIQAGR
                     VTYGATARASEAVEDTFRFRVTAPPYFSPLYTFPIHIGGDPDAPVLTNVLLVVPEGGE
                     GVLSADHLFVKSLNSASYLYEVMERPRHGRLAWRGTQDKTTMVTSFTNEDLLRGRLVY
                     QHDDSETTEDDIPFVATRQGESSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRIFHVA
                     RGGRRLLTTDDVAFSDADSGFADAQLVLTRKDLLFGSIVAVDEPTRPIYRFTQEDLRK
                     RRVLFVHSGADRGWIQLQVSDGQHQATALLEVQASEPYLRVANGSSLVVPQGGQGTID
                     TAVLHLDTNLDIRSGDEVHYHVTAGPRWGQLVRAGQPATAFSQQDLLDGAVLYSHNGS
                     LSPRDTMAFSVEAGPVHTDATLQVTIALEGPLAPLKLVRHKKIYVFQGEAAEIRRDQL
                     EAAQEAVPPADIVFSVKSPPSAGYLVMVSRGALADEPPSLDPVQSFSQEAVDTGRVLY
                     LHSRPEAWSDAFSLDVASGLGAPLEGVLVELEVLPAAIPLEAQNFSVPEGGSLTLAPP
                     LLRVSGPYFPTLLGLSLQVLEPPQHGALQKEDGPQARTLSAFSWRMVEEQLIRYVHDG
                     SETLTDSFVLMANASEMDRQSHPVAFTVTVLPVNDQPPILTTNTGLQMWEGATAPIPA
                     EALRSTDGDSGSEDLVYTIEQPSNGRVVLRGAPGTEVRSFTQAQLDGGLVLFSHRGTL
                     DGGFRFRLSDGEHTSPGHFFRVTAQKQVLLSLKGSQTLTVCPGSVQPLSSQTLRASSS
                     AGTDPQLLLYRVVRGPQLGRLFHAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFW
                     EAHDTLELQLSSPPARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVA
                     ALDASNLLASVPSPQRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQL
                     VYAHGGGGTQQDGFHFRAHLQGPAGASVAGPQTSEAFAITVRDVNERPPQPQASVPLR
                     LTRGSRAPISRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLVGGGLGPVTRFTQADVD
                     SGRLAFVANGSSVAGIFQLSMSDGASPPLPMSLAVDILPSAIEVQLRAPLEVPQALGR
                     SSLSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTN
                     FSSSHDHFRVLALARGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELA
                     NRTGSVPRFRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEG
                     RAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAARTEAGKPE
                     SSTPTGEPGPMASSPEPAVAKGGFLSFLEANMFSVIIPMCLVLLLLALILPLLFYLRK
                     RNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLTAVPGQGPPPGGQPDPELLQ
                     FCRTPNPALKNGQYWV"
     exon            182..345
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            346..3882
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3883..4365
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            4366..4542
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            4543..4741
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            4742..4876
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            4877..5043
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            5044..5227
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     exon            5228..8290
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7963..7968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /note="hexamer: GATAAA"
     polyA_site      7989
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /note="major polyA site"
     regulatory      8269..8274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
                     /note="hexamer: AATAAA"
     polyA_site      8290
                     /gene="CSPG4"
                     /gene_synonym="CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG;
                     MSK16; NG2"
ORIGIN      
        1 gcgcccagga gcagagccgc gctcgctcca ctcagctccc agctcccagg actccgctgg
       61 ctcctcgcaa gtcctgccgc ccagcccgcc gggatgcagt ccgggccgcg gcccccactt
      121 ccagcccccg gcctggcctt ggctttgacc ctgactatgt tggccagact tgcatccgcg
      181 gcttccttct tcggtgagaa ccacctggag gtgcctgtgg ccacggctct gaccgacata
      241 gacctgcagc tgcagttctc cacgtcccag cccgaagccc tccttctcct ggcagcaggc
      301 ccagctgacc acctcctgct gcagctctac tctggacgcc tgcaggtcag acttgttctg
      361 ggccaggagg agctgaggct gcagactcca gcagagacgc tgctgagtga ctccatcccc
      421 cacactgtgg tgctgactgt cgtagagggc tgggccacgt tgtcagtcga tgggtttctg
      481 aacgcctcct cagcagtccc aggagccccc ctagaggtcc cctatgggct ctttgttggg
      541 ggcactggga cccttggcct gccctacctg aggggaacca gccgacccct gaggggttgc
      601 ctccatgcag ccaccctcaa tggccgcagc ctcctccggc ctctgacccc cgatgtgcat
      661 gagggctgtg ctgaagagtt ttctgccagt gatgatgtgg ccctgggctt ctctgggccc
      721 cactctctgg ctgccttccc tgcctggggc actcaggacg aaggaaccct agagtttaca
      781 ctcaccacac agagccggca ggcacccttg gccttccagg cagggggccg gcgtggggac
      841 ttcatctatg tggacatatt tgagggccac ctgcgggccg tggtggagaa gggccagggt
      901 accgtattgc tccacaacag tgtgcctgtg gccgatgggc agccccatga ggtcagtgtc
      961 cacatcaatg ctcaccggct ggaaatctcc gtggaccagt accctacgca tacttcgaac
     1021 cgaggagtcc tcagctacct ggagccacgg ggcagtctcc ttctcggggg gctggatgca
     1081 gaggcctctc gtcacctcca ggaacaccgc ctgggcctga caccagaggc caccaatgcc
     1141 tccctgctgg gctgcatgga agacctcagt gtcaatggcc agaggcgggg gctgcgggaa
     1201 gctttgctga cgcgcaacat ggcagccggc tgcaggctgg aggaggagga gtatgaggac
     1261 gatgcctatg gacattatga agctttctcc accctggccc ctgaggcttg gccagccatg
     1321 gagctgcctg agccatgcgt gcctgagcca gggctgcctc ctgtctttgc caatttcacc
     1381 cagctgctga ctatcagccc actggtggtg gccgaggggg gcacagcctg gcttgagtgg
     1441 aggcatgtgc agcccacgct ggacctgatg gaggctgagc tgcgcaaatc ccaggtgctg
     1501 ttcagcgtga cccgaggggc acgccatggc gagctcgagc tggacatccc gggagcccag
     1561 gcacgaaaaa tgttcaccct cctggacgtg gtgaaccgca aggcccgctt catccacgat
     1621 ggctctgagg acacctccga ccagctggtg ctggaggtgt cggtgacggc tcgggtgccc
     1681 atgccctcat gccttcggag gggccaaaca tacctcctgc ccatccaggt caaccctgtc
     1741 aatgacccac cccacatcat cttcccacat ggcagcctca tggtgatcct ggaacacacg
     1801 cagaagccgc tggggcctga ggttttccag gcctatgacc cggactctgc ctgtgagggc
     1861 ctcaccttcc aggtccttgg cacctcctct ggcctccccg tggagcgccg agaccagcct
     1921 ggggagccgg cgaccgagtt ctcctgccgg gagttggagg ccggcagcct agtctatgtc
     1981 caccgcggtg gtcctgcaca ggacttgacg ttccgggtca gcgatggact gcaggccagc
     2041 cccccggcca cgctgaaggt ggtggccatc cggccggcca tacagatcca ccgcagcaca
     2101 gggttgcgac tggcccaagg ctctgccatg cccatcttgc ccgccaacct gtcggtggag
     2161 accaatgccg tggggcagga tgtgagcgtg ctgttccgcg tcactggggc cctgcagttt
     2221 ggggagctgc agaagcaggg ggcaggtggg gtggagggtg ctgagtggtg ggccacacag
     2281 gcgttccacc agcgggatgt ggagcagggc cgcgtgaggt acctgagcac tgacccacag
     2341 caccacgctt acgacaccgt ggagaacctg gccctggagg tgcaggtggg ccaggagatc
     2401 ctgagcaatc tgtccttccc agtgaccatc cagagagcca ctgtgtggat gctgcggctg
     2461 gagccactgc acactcagaa cacccagcag gagaccctca ccacagccca cctggaggcc
     2521 accctggagg aggcaggccc aagcccccca accttccatt atgaggtggt tcaggctccc
     2581 aggaaaggca accttcaact acagggcaca aggctgtcag atggccaggg cttcacccag
     2641 gatgacatac aggctggccg ggtgacctat ggggccacag cacgtgcctc agaggcagtc
     2701 gaggacacct tccgtttccg tgtcacagct ccaccatatt tctccccact ctataccttc
     2761 cccatccaca ttggtggtga cccagatgcg cctgtcctca ccaatgtcct cctcgtggtg
     2821 cctgagggtg gtgagggtgt cctctctgct gaccacctct ttgtcaagag tctcaacagt
     2881 gccagctacc tctatgaggt catggagcgg ccccgccatg ggaggttggc ttggcgtggg
     2941 acacaggaca agaccactat ggtgacatcc ttcaccaatg aagacctgtt gcgtggccgg
     3001 ctggtctacc agcatgatga ctccgagacc acagaagatg atatcccatt tgttgctacc
     3061 cgccagggcg agagcagtgg tgacatggcc tgggaggagg tacggggtgt cttccgagtg
     3121 gccatccagc ccgtgaatga ccacgcccct gtgcagacca tcagccggat cttccatgtg
     3181 gcccggggtg ggcggcggct gctgactaca gacgacgtgg ccttcagcga tgctgactcg
     3241 ggctttgctg acgcccagct ggtgcttacc cgcaaggacc tcctctttgg cagtatcgtg
     3301 gccgtagatg agcccacgcg gcccatctac cgcttcaccc aggaggacct caggaagagg
     3361 cgagtactgt tcgtgcactc aggggctgac cgtggctgga tccagctgca ggtgtccgac
     3421 gggcaacacc aggccactgc gctgctggag gtgcaggcct cggaacccta cctccgtgtg
     3481 gccaacggct ccagccttgt ggtccctcaa ggaggccagg gcaccatcga cacggccgtg
     3541 ctccacctgg acaccaacct cgacatccgc agtggggatg aggtccacta ccacgtcaca
     3601 gctggccctc gctggggaca gctagtccgg gctggtcagc cagccacagc cttctcccag
     3661 caggacctgc tggatggggc cgttctctat agccacaatg gcagcctcag cccccgcgac
     3721 accatggcct tctccgtgga agcagggcca gtgcacacgg atgccaccct acaagtgacc
     3781 attgccctag agggcccact ggccccactg aagctggtcc ggcacaagaa gatctacgtc
     3841 ttccagggag aggcagctga gatcagaagg gaccagctgg aggcagccca ggaggcagtg
     3901 ccacctgcag acatcgtatt ctcagtgaag agcccaccga gtgccggcta cctggtgatg
     3961 gtgtcgcgtg gcgccttggc agatgagcca cccagcctgg accctgtgca gagcttctcc
     4021 caggaggcag tggacacagg cagggtcctg tacctgcact cccgccctga ggcctggagc
     4081 gatgccttct cgctggatgt ggcctcaggc ctgggtgctc ccctcgaggg cgtccttgtg
     4141 gagctggagg tgctgcccgc tgccatccca ctagaggcgc aaaacttcag cgtccctgag
     4201 ggtggcagcc tcaccctggc ccctccactg ctccgtgtct ccgggcccta cttccccact
     4261 ctcctgggcc tcagcctgca ggtgctggag ccaccccagc atggagccct gcagaaggag
     4321 gacggacctc aagccaggac cctcagcgcc ttctcctgga gaatggtgga agagcagctg
     4381 atccgctacg tgcatgacgg gagcgagaca ctgacagaca gttttgtcct gatggctaat
     4441 gcctccgaga tggatcgcca gagccatcct gtggccttca ctgtcactgt cctgcctgtc
     4501 aatgaccaac cccccatcct cactacaaac acaggcctgc agatgtggga gggggccact
     4561 gcgcccatcc ctgcggaggc tctgaggagc acggacggcg actctgggtc tgaggatctg
     4621 gtctacacca tcgagcagcc cagcaacggg cgggtagtgc tgcggggggc gccgggcact
     4681 gaggtgcgca gcttcacgca ggcccagctg gacggcgggc tcgtgctgtt ctcacacaga
     4741 ggaaccctgg atggaggctt ccgcttccgc ctctctgacg gcgagcacac ttcccccgga
     4801 cacttcttcc gagtgacggc ccagaagcaa gtgctcctct cgctgaaggg cagccagaca
     4861 ctgactgtct gcccagggtc cgtccagcca ctcagcagtc agaccctcag ggccagctcc
     4921 agcgcaggca ctgaccccca gctcctgctc taccgtgtgg tgcggggccc ccagctaggc
     4981 cggctgttcc acgcccagca ggacagcaca ggggaggccc tggtgaactt cactcaggca
     5041 gaggtctacg ctgggaatat tctgtatgag catgagatgc cccccgagcc cttttgggag
     5101 gcccatgata ccctagagct ccagctgtcc tcgccgcctg cccgggacgt ggccgccacc
     5161 cttgctgtgg ctgtgtcttt tgaggctgcc tgtccccagc gccccagcca cctctggaag
     5221 aacaaaggtc tctgggtccc cgagggccag cgggccagga tcaccgtggc tgctctggat
     5281 gcctccaatc tcttggccag cgttccatca ccccagcgct cagagcatga tgtgctcttc
     5341 caggtcacac agttccccag ccggggccag ctgttggtgt ccgaggagcc cctccatgct
     5401 gggcagcccc acttcctgca gtcccagctg gctgcagggc agctagtgta tgcccacggc
     5461 ggtgggggca cccagcagga tggcttccac tttcgtgccc acctccaggg gccagcaggg
     5521 gcctccgtgg ctggacccca aacctcagag gcctttgcca tcacggtgag ggatgtaaat
     5581 gagcggcccc ctcagccaca ggcctctgtc ccactccggc tcacccgagg ctctcgtgcc
     5641 cccatctccc gggcccagct gagtgtggtg gacccagact cagctcctgg ggagattgag
     5701 tacgaggtcc agcgggcacc ccacaacggc ttcctcagcc tggtgggtgg tggcctgggg
     5761 cccgtgaccc gcttcacgca agccgatgtg gattcagggc ggctggcctt cgtggccaac
     5821 gggagcagcg tggcaggcat cttccagctg agcatgtctg atggggccag cccacccctg
     5881 cccatgtccc tggctgtgga catcctacca tccgccatcg aggtgcagct gcgggcaccc
     5941 ctggaggtgc cccaagcttt ggggcgctcc tcactgagcc agcagcagct ccgggtggtt
     6001 tcagatcggg aggagccaga ggcagcatac cgcctcatcc agggacccca gtatgggcat
     6061 ctcctggtgg gcgggcggcc cacctcggcc ttcagccaat tccagataga ccagggcgag
     6121 gtggtctttg ccttcaccaa cttctcctcc tctcatgacc acttcagagt cctggcactg
     6181 gctaggggtg tcaatgcatc agccgtagtg aacgtcactg tgagggctct gctgcatgtg
     6241 tgggcaggtg ggccatggcc ccagggtgcc accctgcgcc tggaccccac cgtcctagat
     6301 gctggcgagc tggccaaccg cacaggcagt gtgccgcgct tccgcctcct ggagggaccc
     6361 cggcatggcc gcgtggtccg cgtgccccga gccaggacgg agcccggggg cagccagctg
     6421 gtggagcagt tcactcagca ggaccttgag gacgggaggc tggggctgga ggtgggcagg
     6481 ccagagggga gggcccccgg ccccgcaggt gacagtctca ctctggagct gtgggcacag
     6541 ggcgtcccgc ctgctgtggc ctccctggac tttgccactg agccttacaa tgctgcccgg
     6601 ccctacagcg tggccctgct cagtgtcccc gaggccgccc ggacggaagc agggaagcca
     6661 gagagcagca cccccacagg cgagccaggc cccatggcat ccagccctga gcccgctgtg
     6721 gccaagggag gcttcctgag cttccttgag gccaacatgt tcagcgtcat catccccatg
     6781 tgcctggtac ttctgctcct ggcgctcatc ctgcccctgc tcttctacct ccgaaaacgc
     6841 aacaagacgg gcaagcatga cgtccaggtc ctgactgcca agccccgcaa cggcctggct
     6901 ggtgacaccg agacctttcg caaggtggag ccaggccagg ccatcccgct cacagctgtg
     6961 cctggccagg ggccccctcc aggaggccag cctgacccag agctgctgca gttctgccgg
     7021 acacccaacc ctgcccttaa gaatggccag tactgggtgt gaggcctggc ctgggcccag
     7081 atgctgatcg ggccagggac aggcttgccc atgtcccggg ccccattgct tccatgcctg
     7141 gtgctgtctg agtatcccca gagcaagaga gacctggaga caccaggggt ggagggtcct
     7201 gggagatagt cccaggggtc cgggacagag tggagtcaag agctggaacc tccctcagct
     7261 cactccgagc ctggagaact gcaggggcca aggtggaggc aggcttaagt tcagtcctcc
     7321 tgccctggag ctggtttggg ctgtcaaaac cagggtaacc tcctacatgg gtcatgactc
     7381 tgggtcctgg gtctgtgacc ttgggtaagt cgcgcctgac ccaggctgct aagagggcaa
     7441 ggagaaggaa gtaccctggg gagggaaggg acagaggaag ctattcctgg cttttccact
     7501 ccaacccagg ccaccctttg tctctgcccc agagttgaga aaaaaacttc ctcccctggt
     7561 tttttaggga gatggtatcc cctggagtag agggcaagag gagagagcgc ctccagtcta
     7621 gaaggcataa gccaatagga taatatattc agggtgcagg gtgggtaggt tgctctgggg
     7681 atgggtttat ttaagggaga ttgcaaggaa gctatttaac atggtgctga gctagccagg
     7741 actgatggag cccctggggg tgtgggatgg aggagggtct gcagccagtt cattcccagg
     7801 gccccatctt gatgggccaa gggctaaaca tgcatgtgtc agtggctttg gagcaggtta
     7861 ggctggggct catcgagggt ctcaggccga ggccactgcg gtgccagtgc ccccctgagg
     7921 actagggcag gcagctgggg gcacttggtt ccatggagcc tggataaaca gtgctttgga
     7981 ggctctggac agctgtgtgg tgtttgtgtc ttaactatgc actgggccct tgtctgcgtc
     8041 ggcttgcata cagagggccc ctggggtcgg ccctccggcc tggcctcagc cagtgggatg
     8101 gacagggcca ggcaggcctc tgaacttcca cctcctgggg cctcccagac ctcctgtgcc
     8161 cccacctgtg tgggcaggtg ggccagtctt cgggtgatgg gaccaaaccc cttcagttca
     8221 gtagagaaag gctaggtcct ctacaaagag ctgcaagaca aaaattaaaa taaatgctcc
     8281 ccaccctaga
//
