LOCUS       NM_177559              12984 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens casein kinase 2 alpha 1 (CSNK2A1), transcript variant
            1, mRNA.
ACCESSION   NM_177559
VERSION     NM_177559.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Goel,H. and O'Donnell,S.
  TITLE     Inherited loss of function variant in CSNK2A1: the oldest reported
            cases of Okur-Chung syndrome in a single family
  JOURNAL   Clin Dysmorphol 33 (3), 121-124 (2024)
   PUBMED   38818820
  REMARK    GeneRIF: Inherited loss of function variant in CSNK2A1: the oldest
            reported cases of Okur-Chung syndrome in a single family.
REFERENCE   2  (bases 1 to 12984)
  AUTHORS   Ampofo,E., Pack,M., Wrublewsky,S., Boewe,A.S., Spigelman,A.F.,
            Koch,H., MacDonald,P.E., Laschke,M.W., Montenarh,M. and Gotz,C.
  TITLE     CK2 activity is crucial for proper glucagon expression
  JOURNAL   Diabetologia 67 (7), 1368-1385 (2024)
   PUBMED   38503901
  REMARK    GeneRIF: CK2 activity is crucial for proper glucagon expression.
REFERENCE   3  (bases 1 to 12984)
  AUTHORS   Liu,Z.D., Shi,Y.H., Xu,Q.C., Zhao,G.Y., Zhu,Y.Q., Li,F.X., Ma,M.J.,
            Ye,J.Y., Huang,X.T., Wang,X.Y., Xu,X., Wang,J.Q., Zhao,W. and
            Yin,X.Y.
  TITLE     CSNK2A1 confers gemcitabine resistance to pancreatic ductal
            adenocarcinoma via inducing autophagy
  JOURNAL   Cancer Lett 585, 216640 (2024)
   PUBMED   38290659
  REMARK    GeneRIF: CSNK2A1 confers gemcitabine resistance to pancreatic
            ductal adenocarcinoma via inducing autophagy.
REFERENCE   4  (bases 1 to 12984)
  AUTHORS   Nan,H., Chu,M., Zhang,J., Jiang,D., Wang,Y. and Wu,L.
  TITLE     Okur-Chung neurodevelopmental syndrome: Implications for phenotype
            and genotype expansion
  JOURNAL   Mol Genet Genomic Med 12 (3), e2398 (2024)
   PUBMED   38444259
  REMARK    GeneRIF: Okur-Chung neurodevelopmental syndrome: Implications for
            phenotype and genotype expansion.
            Review article
REFERENCE   5  (bases 1 to 12984)
  AUTHORS   Lee,C.Y., Hubrich,D., Varga,J.K., Schafer,C., Welzel,M.,
            Schumbera,E., Djokic,M., Strom,J.M., Schonfeld,J., Geist,J.L.,
            Polat,F., Gibson,T.J., Keller Valsecchi,C.I., Kumar,M.,
            Schueler-Furman,O. and Luck,K.
  TITLE     Systematic discovery of protein interaction interfaces using
            AlphaFold and experimental validation
  JOURNAL   Mol Syst Biol 20 (2), 75-97 (2024)
   PUBMED   38225382
REFERENCE   6  (bases 1 to 12984)
  AUTHORS   Chung,W. and Okur,V.
  TITLE     Okur-Chung Neurodevelopmental Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   35679446
REFERENCE   7  (bases 1 to 12984)
  AUTHORS   Hagiwara,T., Nakaya,K., Nakamura,Y., Nakajima,H., Nishimura,S. and
            Taya,Y.
  TITLE     Specific phosphorylation of the acidic central region of the N-myc
            protein by casein kinase II
  JOURNAL   Eur J Biochem 209 (3), 945-950 (1992)
   PUBMED   1425701
REFERENCE   8  (bases 1 to 12984)
  AUTHORS   Russo,G.L., Vandenberg,M.T., Yu,I.J., Bae,Y.S., Franza,B.R. Jr. and
            Marshak,D.R.
  TITLE     Casein kinase II phosphorylates p34cdc2 kinase in G1 phase of the
            HeLa cell division cycle
  JOURNAL   J Biol Chem 267 (28), 20317-20325 (1992)
   PUBMED   1400350
REFERENCE   9  (bases 1 to 12984)
  AUTHORS   Lin,A., Frost,J., Deng,T., Smeal,T., al-Alawi,N., Kikkawa,U.,
            Hunter,T., Brenner,D. and Karin,M.
  TITLE     Casein kinase II is a negative regulator of c-Jun DNA binding and
            AP-1 activity
  JOURNAL   Cell 70 (5), 777-789 (1992)
   PUBMED   1516134
  REMARK    Erratum:[Cell. 1992 Nov 27;71(5):887.. PMID: 1423636]
REFERENCE   10 (bases 1 to 12984)
  AUTHORS   Sacks,D.B., Davis,H.W., Crimmins,D.L. and McDonald,J.M.
  TITLE     Insulin-stimulated phosphorylation of calmodulin
  JOURNAL   Biochem J 286 (Pt 1) (Pt 1), 211-216 (1992)
   PUBMED   1520270
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AU132198.1, M55265.1,
            BC053532.1 and AL049761.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_177559.2.
            
            Summary: Casein kinase II is a serine/threonine protein kinase that
            phosphorylates acidic proteins such as casein. It is involved in
            various cellular processes, including cell cycle control,
            apoptosis, and circadian rhythm. The kinase exists as a tetramer
            and is composed of an alpha, an alpha-prime, and two beta subunits.
            The alpha subunits contain the catalytic activity while the beta
            subunits undergo autophosphorylation. The protein encoded by this
            gene represents the alpha subunit. Multiple transcript variants
            encoding different protein isoforms have been found for this gene.
            [provided by RefSeq, Apr 2018].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a). Variants 1, 2, 4 and 5 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.35161.1,
                                           SRR1803613.132695.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000217244.9/ ENSP00000217244.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-673               AU132198.1         1-673
            674-2392            M55265.1           477-2195
            2393-2788           BC053532.1         2143-2538
            2789-12984          AL049761.11        15579-25774         c
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..12984
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /note="casein kinase 2 alpha 1"
                     /db_xref="GeneID:1457"
                     /db_xref="HGNC:HGNC:2457"
                     /db_xref="MIM:115440"
     exon            1..119
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            120..236
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            237..446
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     CDS             346..1521
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1; CK2
                     catalytic subunit alpha; protein kinase CK2; casein kinase
                     II alpha 1 subunit; casein kinase II alpha 1 polypeptide
                     pseudogene; casein kinase 2, alpha 1 polypeptide"
                     /codon_start=1
                     /product="casein kinase II subunit alpha isoform a"
                     /protein_id="NP_808227.1"
                     /db_xref="CCDS:CCDS13003.1"
                     /db_xref="GeneID:1457"
                     /db_xref="HGNC:HGNC:2457"
                     /db_xref="MIM:115440"
                     /translation="MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRK
                     LGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVK
                     DPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPH
                     NVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSL
                     GCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRH
                     SRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARM
                     GSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ"
     exon            447..558
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            559..660
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            661..711
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            712..771
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            772..855
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            856..966
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1068
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1169
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1318
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1405
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..12984
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2763..2768
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /note="hexamer: AATAAA"
     polyA_site      2788
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /note="major polyA site"
     regulatory      4351..4356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /note="hexamer: AATAAA"
     regulatory      4365..4370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /note="hexamer: AATAAA"
     polyA_site      4375
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
     polyA_site      4385
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
     regulatory      10956..10961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
                     /note="hexamer: AATAAA"
     polyA_site      10978
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
     polyA_site      12984
                     /gene="CSNK2A1"
                     /gene_synonym="CK2A1; Cka1; Cka2; CKII; OCNDS"
ORIGIN      
        1 agcctcgctc tggtccctgc ggctggcggc cgagccgtgt gtctcctcct ccatcgccgc
       61 catattgtct gtgtgagcag aggggagagc ggccgccgcc gctgccgctt ccaccacagc
      121 tctatcaagg cttgtcaagc agtgtgctca tcacatggta aatcatgcag cgtggaacct
      181 cataaaatct ccaagaaaca tcattcaccc atactgacta gtttcacatc tctttgtttg
      241 aagaaaacag gtctgaaaca aggtcttacc cccagctgct tctgaacaca gtgactgcca
      301 gatctccaaa catcaagtcc agctttgtcc gccaacctgt ctgacatgtc gggacccgtg
      361 ccaagcaggg ccagagttta cacagatgtt aatacacaca gacctcgaga atactgggat
      421 tacgagtcac atgtggtgga atggggaaat caagatgact accagctggt tcgaaaatta
      481 ggccgaggta aatacagtga agtatttgaa gccatcaaca tcacaaataa tgaaaaagtt
      541 gttgttaaaa ttctcaagcc agtaaaaaag aagaaaatta agcgtgaaat aaagattttg
      601 gagaatttga gaggaggtcc caacatcatc acactggcag acattgtaaa agaccctgtg
      661 tcacgaaccc ccgccttggt ttttgaacac gtaaacaaca cagacttcaa gcaattgtac
      721 cagacgttaa cagactatga tattcgattt tacatgtatg agattctgaa ggccctggat
      781 tattgtcaca gcatgggaat tatgcacaga gatgtcaagc cccataatgt catgattgat
      841 catgagcaca gaaagctacg actaatagac tggggtttgg ctgagtttta tcatcctggc
      901 caagaatata atgtccgagt tgcttcccga tacttcaaag gtcctgagct acttgtagac
      961 tatcagatgt acgattatag tttggatatg tggagtttgg gttgtatgct ggcaagtatg
     1021 atctttcgga aggagccatt tttccatgga catgacaatt atgatcagtt ggtgaggata
     1081 gccaaggttc tggggacaga agatttatat gactatattg acaaatacaa cattgaatta
     1141 gatccacgtt tcaatgatat cttgggcaga cactctcgaa agcgatggga acgctttgtc
     1201 cacagtgaaa atcagcacct tgtcagccct gaggccttgg atttcctgga caaactgctg
     1261 cgatatgacc accagtcacg gcttactgca agagaggcaa tggagcaccc ctatttctac
     1321 actgttgtga aggaccaggc tcgaatgggt tcatctagca tgccaggggg cagtacgccc
     1381 gtcagcagcg ccaatatgat gtcagggatt tcttcagtgc caaccccttc accccttgga
     1441 cctctggcag gctcaccagt gattgctgct gccaaccccc ttgggatgcc tgttccagct
     1501 gccgctggcg ctcagcagta acggccctat ctgtctcctg atgcctgagc agaggtgggg
     1561 gagtccaccc tctccttgat gcagcttgcg cctggcgggg aggggtgaaa cacttcagaa
     1621 gcaccgtgtc tgaaccgttg cttgtggatt tatagtagtt cagtcataaa aaaaaaatta
     1681 taataggctg attttctttt ttcttttttt ttttaactcg aacttttcat aactcagggg
     1741 attccctgaa aaattacctg caggtggaat atttcatgga caaatttttt tttctcccct
     1801 cccaaattta gttcctcatc acaaaagaac aaagataaac cagcctcaat cccggctgct
     1861 gcatttaggt ggagacttct tcccattccc accattgttc ctccaccgtc ccacacttta
     1921 gggggttggt atctcgtgct cttctccaga gattacaaaa atgtagcttc tcaggggagg
     1981 caggaagaaa ggaaggaagg aaagaaggaa gggaggaccc aatctatagg agcagtggac
     2041 tgcttgctgg tcgcttacat cactttactc cataagcgct tcagtggggt tatcctagtg
     2101 gctcttgtgg aagtgtgtct tagttacatc aagatgttga aaatctaccc aaaatgcaga
     2161 cagatactaa aaacttctgt tcagtaagaa tcatgtctta ctgatctaac cctaaatcca
     2221 actcatttat acttttattt ttagttcagt ttaaaatgtt gataccttcc ctcccaggct
     2281 ccttaccttg gtcttttccc tgttcatctc ccaacatgct gtgctccata gctggtagga
     2341 gagggaaggc aaaatctttc ttagttttct ttgtcttggc cattttgaat tcatttagtt
     2401 actgggcata acttactgct ttttacaaaa gaaacaaaca ttgtctgtac aggtttcatg
     2461 ctagagctaa tgggagatgt ggccacactg acttccattt taagctttct accttctttt
     2521 cctccgaccg tccccttccc tcacatgcca tccagtgaga agacctgctc ctcagtcttg
     2581 taaatgtatc ttgagaggta ggagcagagc cactatctcc attgaagctg aaatggtaga
     2641 cctgtaattg tgggaaaact ataaactctc ttgttacagc cccgccaccc cttgctgtgt
     2701 gtatatatat aatactttgt ccttcatatg tgaaagatcc agtgttggaa ttctttggtg
     2761 taaataaacg tttggtttta tttatcaagg ttagatttaa gttccctgtg taaaggtctt
     2821 gctgggtggg tgtctcatgt tcacatctga ggggcctgca gccctgtacc gtggaggctt
     2881 cccaaggccc ccattttata cacccctcgt tcgacccatg gtaccgggca gagcagagag
     2941 gccttaaaaa aaaagcacca caagccaaag cgtctctggg gattaaggat cctttgccat
     3001 aaaactcatt ttgtgtctca gcagtgcagg gattggggat ggaaaaagtc gccagttttt
     3061 gtgtgtttgt gtgtgtgtgt aaagtggact ttccactgta atccaaccac ctaagtttat
     3121 caggtgcttc actgaggaag cctagttttt taagcacaat agcaaaacca tcagctctgt
     3181 attttctcct gttatttcat tacagtagct gcttgtggga actaggaaaa attcttccaa
     3241 catattttaa ggcctaaaat cttagttccc cattctccta ccttatagat tcacaggcct
     3301 ttctcgccta ggcatcatag ataaacgtaa ttgtttgggg agttgaattt aatgaactta
     3361 tctaactttg taacccatct tggctttagt aactttatca aggtggtggc tttagtgaat
     3421 ataatggtaa actttagagg acgctaaagc ctccttttat agcgcttctc aacggtaggg
     3481 agagctgaag ggaaaacatt ctgactgtgt ggcagggtga ttttcttcct tatgaccact
     3541 tacagtggat atttattgta cttgaccctt ttatgcccta gaatgctgtg agggttacca
     3601 tgttgaattt gtgcagaagc taaaagcacc agatgtgcca gagatgcaat ttgtgattat
     3661 gtttgcactg gattgtgatt tgaacaggac acttataact aatgagttct ttcttttgag
     3721 gtggggagag ggttgtaaat caagacttca taccctatcc ttgtagctcg gaaattgagg
     3781 tgtagcttag gctgatgcgg agagctgcag acagctggac ctccttacac ccatcagacc
     3841 ttggaataaa ccgaaggtga atgcagagga ctctgaggtg ccacccaaga gccttttaaa
     3901 ataaatagaa gtttttaaaa atgctactta agagtcactg cagaagcatg gagagagaga
     3961 aaaaaaaaaa agacccaatt gggtgggggg tgggggggag gcaagggttt acagagtaac
     4021 aacttacgtg ttgttgtaaa ttacctcatc tgtatacctt gtattgggac actatttctc
     4081 agcactacct gtgtctgcat tgcttagatg gcaggaaatg gaatggccaa catgaaaggt
     4141 tatgttggca attactctga aagatttttt aaagtatttt taaactttgt ttgaagtggt
     4201 ccctcccctc ttcctctgct tttttttaag tttgaagcca gaaggccctc cccaccttgc
     4261 tttattggtt gtaattgtat tgtaattggt atgactaaac cataactgtg ctgggaagaa
     4321 gttgtgccat gaaggtcttt aatttttttt aataaaaaca tttaaataaa tgaaatgtcc
     4381 ctgcagcctg agcaatatgc ttctccaacc gacaccgtgt gtgatagatg gctccgtttt
     4441 cacgttttga attctgtttc ttgtacttga aatgcctgtc cagcttacat aagatagatt
     4501 aaattgttag agtgggactg tcagaggaat atatcttgta aagtagatcc tcagggtatg
     4561 gctaattact atagtcctaa agtagcagag aaaattagag attggttgac agatttggga
     4621 attttaattt cttcttagtt tttaactttc atctttgctt caccttttga caagtatggt
     4681 tataattaga attgaccatt taagcatctc ttgaaaaacc tgattttgtc ccaccagcaa
     4741 attctctccc aagactggaa ttgggtttgc atagtagaga gcctgtcttc ccagggcctt
     4801 ttcctagtta aagaaacact taactaaaaa ggctgtgtgg taaggtcatg ctcagttcct
     4861 tcctcaaaaa atgagtctaa aattggttta tcctgaatcc gacttccccc actgtcatca
     4921 cttacccttg cccttctttt tcctcagaac acctgaactt ttgttgtatt attttgtatc
     4981 tccccacttg catgtaaact ccctggtgac cttgcatgtc ttatgcaatc ctcattaaaa
     5041 ccttttgttt ttttttttaa gtgggttcgt tttccctcag gatcgaagag tattgaatgg
     5101 aatggcacga ggaaaatatt taacacccta cttggcttgt aataataggt gcttaataaa
     5161 ttgtagttcc actctccact ccattgcccc ctttctctcc ctacacacac acacccaccc
     5221 acccacacac acacacacac acacacaaag taaatggaag tattgttgca taaagagcat
     5281 ctcaaaatcc taagaacttt gcccttgagt ttgcagctat aattacaagc cacaggccag
     5341 actgaactaa catgacaagt aactttattt aatgagaccc tgataaatag gtatctgaag
     5401 actaaggaat gatcctggcc aatttagtag acttagatgc tgcagtctcc ctgcttttcc
     5461 cctgctgtat tacctgtgtg taatataggt tgaatatccc ctatccaaaa tggttgggac
     5521 cagaagtgtt ttgaattttt ttggattttt ggaatatttg cggaatacat accagttcaa
     5581 tattcctaat ctgaaatttg aaatattcca aatagcattt actttaggca tcatgttgat
     5641 gcttaaaaaa tttcagattt ttgaggattt tgtattttca aattagaaat gctcaacgtg
     5701 taatacgaaa ttcagactat tctccaagta aaacatatgt agatggtgtc acaagatgtt
     5761 ctggactcaa tcctatgtaa ataagtactt cctagacttt atagctctcc tttgattctg
     5821 tgctaaattt aaccattaga gaagcaatgt aatgttactg caagtggtct acctctaaca
     5881 gagttctgtc tggcagtgtg atttttttga tcagttaacc tctctgaatc ttttcttcac
     5941 aggtgattta aattgttatt gtaaaacatt ttaggcatgt accaaattat aaagaaagct
     6001 gtaacacatc cataatccta ccacccaagt tgaatttcca ttgccaatac agaccaagcc
     6061 ccctttttgc ccttttttgg tgataccctc ttcttcctcc cttccccaga ggtcccaata
     6121 tttcctatct gttaaattgg ggtataagta ctgatttcat agagcggttg ttcagattct
     6181 atgacagatg cctggtatat ggtagagtta caccaaatgt tggtttctct tctaaattgt
     6241 acagttgtgc agactatgta cagtaaaagg atgctacatt atagggaggc catagatgtt
     6301 tgcatttgga aaatttctga ctcttttcca gcaggtggca gtaaagtgtc tttactagaa
     6361 gaaaaaatat ccaagcatta aacatgacag gttttcaacg ggtacaagtc aagtttctga
     6421 ttgaatgaag gccagcattg ccttgttccc actccctcta tggaaagcca gatattttgc
     6481 actgtagcaa attacaaact ggatttgtct ccctgtcgct ctactcacta gaagtttttt
     6541 gtttgttttg ttttttgata cgggtcttgc tctgttaccc aggctagagt gcagtagcgc
     6601 catcacagct cactgcagcc tcaacctccc aggctcaggt gatcctccca actcagcctt
     6661 ccagggacta caggcacgag ccatcatgct tggctaattt tttttttttt ttttttggta
     6721 gagatgaagt tttgccgtgt tgcccaggct ggtcttgaac tcctgagctc aacagtctac
     6781 ccgccttggc ctcccaaagt gctgagatta gaggcatgag ccaccacacc tggcccacta
     6841 gggattctta ttggcctcaa tgtgagaagc ccttccccag gtacctccag atggaagctt
     6901 acaggatggt cagtccattt cttagagaag ttcctggggg gattgagtat ttctgagtca
     6961 gcttcactct tgcatcatct gctttgacag ttttcttggc tcctgtaatg agaaaaaggg
     7021 aaaaaaatgc tgcagcagct ggggctggct tcctgatccc accctgggaa tcagagtctc
     7081 agctattgat aaacatttct agccatacct agaaggagag gaccttgctt ctgtgtgttg
     7141 aacagttcca agaactccat ggtgaacttc ctatgaagaa ccctccataa ccctcaaccc
     7201 ttcaaacctg acggtatacc tcattttgtt ctcccgtctc tggcattcct cacccaccca
     7261 cgctgactgg tctttccaaa gttcatttct gtaatatagg atgtctttgg tttcaacaaa
     7321 catttgtaag tgcccctgag cctggtcttt gctatacact ggggattgag aggtgagaca
     7381 caagcccatg gggaaatgta tgttaattag aaaagtgaaa aaattgtgta tatgggataa
     7441 ttccaactgt tttaaaaaaa aaatcctagg aaaaatgcaa atgcctattt cagagaaaga
     7501 aatggaaatc ccaaggtggc aaaaaggaag gcagaattca tcctgaatgt aactggggtt
     7561 tttttgtttt gttttgtttt gtttgtttgt tttgtttttg agacaggcat gggattacat
     7621 gcaccaccac gcccagctaa ttttgtattt ttagtagaga cggggtttct ccatgttggt
     7681 caggctggtc tcgaactccc gacctcaggt gatccgcccg cccaagcctc ccaaagtggt
     7741 gggattacag gcgtgagcca ctgcgcccag cctaagtggg tattaaagtc agtgcttcca
     7801 agacactggg tctgggtata ggtgggcctc aggctatgga ctagggtttg aatgccccca
     7861 gggagatagg acacagactc ccgggctttc taagatagaa actgaaacaa tgctgtggca
     7921 cttggaaagg ctacaggggc aggattgagg tggtgggggt ggtaaaaact agaagaatac
     7981 tagctgttgt tgcctctggg tgatgaggtt aagataccac ggaaggccca cacacctgtg
     8041 gttggaacac tttgggagcc gggagtccca gaccagcctg gacaacatgg cgaaaaccca
     8101 cctctacaaa aaatagatta gctgagtgtg gtggtgtgcg cctgtgtcct agctactggg
     8161 gaggccaaag gacgagaatt aattgcctga gcccaagaag tcaaggctgc agtgaggtgt
     8221 gattgtgcca cagtactcaa gcccaagtga cagagcaaga ccctgtctaa aaagattttt
     8281 ttaaagtcac agaagctggg cgtggtggct catgcctgta atctcagctc tttgggaggc
     8341 tgaggcagga ggattgcttg aggccacgag ttggagacca gcctgggcaa catagggaga
     8401 ccctgtctct accaaaatta aaaattagct ggatgtggtg gcacatgcct gtagtgctag
     8461 ctactctgga ggctgaggca ggaggatcac ttgagcccag gggttcaagg ctgcagtgag
     8521 ctatgatagt gccactgcac cccagactga gcaacagagt gagaccctgt ctgtaaaaga
     8581 agttgttatt aattgaaaaa aagatttaac aggctgggtg cagtggctca cacctgtaat
     8641 ctcagcactt tgagaggtgg aggcgggcga ctcacttgag ctcaggaatt caaggccggt
     8701 ctgggaaaca tgatgaaacc ctctctacaa aaaatacaca gattagctgg gcatggtggt
     8761 gcatgcctgt agtcccagct acttgggagg ctgagatgga ggaatcaatt gagcccagga
     8821 ggttgaggct gcagtgagcc atgatttcac cactgcactc cagcctgtgc aacataggga
     8881 gaccccgcct ccaaaaaaaa aagtaacaga aaaactcact aggtgccggg catggtggct
     8941 cacgcctgta atccaacact ttgggagacc aaggcgagtg gatcacctga ggtcaggagt
     9001 ttgaaaccag actgaacaac atggtgacac cctgtttcta ctaaaaaata caaaaattag
     9061 ctgggcttgg tgacgtgtgc ctgcagtccc agctacttgg gaggctgagg caggagaatt
     9121 gcttgagcct gggaggtggc ggttacagtg agctgagatc acgtcagtgt actccagcct
     9181 gggtgacaga gccagactcc atctaaaagt aaaaaagaaa aactcactag gaaggaatct
     9241 tgaagtcttg gagaagagag aatggtccat gaggcctcag aggaggggct gcctgaactg
     9301 gcgagggaga ggcccagcct aacaagtttc agctgccaag gcccatgcct gggatcagaa
     9361 ccctgtctcc tggctcaatt ttgcttgtgg ggctgctttt gcacagctat gttgccagga
     9421 atctgtccag gttcctagcc agtcattgca gacaaatttt ttctttcttt tgcttttgtt
     9481 ctctgtctag tcttagtctc acaaatttct aaggcaactg tatgtagttc agctgatcta
     9541 gatcaagttc agcccaagca gctgtttttc aggttgtggg tcggctgggc tggacacctc
     9601 catgtgaatt ggattcaggt ttgcccaatg tggcagaagt tgcagcagct actcagggcg
     9661 tgctcttctc atgaatcaca gaaacttaga agagaccaaa tggaatcaca caagcacatt
     9721 tcaagcctct gctgtgtcac aagcaccatc atcccattgg cgaaagcaaa tcacatagcc
     9781 aagcccaaca tcagtgcagt agggaagcat agagcctgtg ttgggaaaac tttatgaggt
     9841 tatatggcaa agggtgtgta tgtttagtct tattacaggg gaatgaagaa acaaaccaat
     9901 aatgcaatca gccatactat tcatatatat cagtaggaac agaaggcaac cacatataac
     9961 atctatgtat atatatatgt gtgtgtggaa aggattacac caactttttt tttttttttt
    10021 tgagtcagag tcttaactct gtcacccagg ctggagtgcg gtggcatgat ctcagcccac
    10081 tgcaacctcc acctcccagg ttcaagcgat tctcctgcct cagcctccca agtagttggg
    10141 attataggca catgccacca cacccagata gtttttgtat ttttacaaaa caacagggtt
    10201 tcaccatgtt ggccaggctg gtctggaact cctgacatca agtaatctgc ctgcctcggc
    10261 ctccaaaagt gctgagatta taggcgtgag ccaccgtgcc cggctgagaa tacttgttta
    10321 cttgttttca agcaacaaaa attgagaatc agtctttgag actccatggc cagaaacaac
    10381 acaacattcc actatacaca gggtgcttct gggggtcctc ggtgttgtgt ttattggggt
    10441 ggtacctaaa tgttgtattt gctctataat tatttgttaa actctccatt tacgtttggg
    10501 gcacttctca gtatatttgc tagagttcaa atgtttaaaa gtttacaaaa tgaaaactga
    10561 gcagagtaag acaagccaaa aaagtattaa ctcagcagga atgggaaaag ttatgaaagg
    10621 agggaaaact aaatatcata ctgctggttc agctgtgatt agaatttacg tagtcataat
    10681 ggaaacactg aatactggtc taaccaggat tacagtatga tgacaatgga aagaggagtg
    10741 atgggaaatg ctggcatgtg tggtggtgtt ggtaacagga caagcggtct aagctctaaa
    10801 ctctcatcat ttataagggg aactaatgcc taaaactgaa aaatcaaaca gttgcatgtt
    10861 attttgagat tttttaaatt attgttaaga tagtagcagt atttactttt aaaactatat
    10921 ttatgtatta ctctggtgaa aaagcaacta cttttaataa atggatattt atcttgaatg
    10981 catccacttc ctcccattcc cgttgccaaa ttcctcatcc cagccaccat cctctcttgc
    11041 ttggagtatc atagcagata actggtcttg atgccttccc atctcgtctc aattcctgat
    11101 tcatctgaca cctgcaacca aagatctttc aaaaataaat attctagtcc aggcacagtg
    11161 actcatatct ataaccccag cattttggga ggccaaggcc cgatgatcac ttgaggcctg
    11221 ggtaacacag cgagtcctct gttctacaaa aaatgaaaac attaactagt gtggtggtgt
    11281 gtgactatag tgctagctac tggggaggct gggtgggagg atacgttgag cccaggagtt
    11341 ggaggctgca gtgagctatg atcacattgc tctcagtgag acctggctga cagagtgaga
    11401 acctctctct taaaaataat aaataaattt tatctcagtc ccttgcttaa aagcctttca
    11461 aagactagat cacttgcaat gttgttgtct cctcccaagg gagtcattcc tcccttcctc
    11521 tttgttgaca gagccccagt ttgagggaca gcgggatatc caccatgctg ctgtgagact
    11581 cgggcagttg tgactggttt agaagtggat acacagggag aggtcttctg gaaaaggtct
    11641 tctggagagc tgagatcaga agacaatcaa ctggcttcaa atctaaggag agacaggcac
    11701 aaaatggaag agatgacatg ggcactggtt acctggtaaa cacaagtgga cactggtgag
    11761 ggactgggtc atggagactg cagtagtctt tacccttttc agcgtttttc accatgaacc
    11821 ccactgggag cacacagaag attggagaaa gttttaataa aatgtctcaa gatctggaag
    11881 aagacaatgg cagctactgt gaaaggatca cagtgctcca cccagaccct tccctgtatc
    11941 ccctaccgga caaaagcctt agtctgtgga gggaaggaca ccaaatcctg tcaccccctt
    12001 ggatgaagct cccttgcatc tgggggaatg aaacaggaac aaaacaaaac tttccctggg
    12061 caggggcagg aatacacact gggcccagaa ctgggggagg aacaggagca ctaaggagga
    12121 cataacccca aaatcagaga cacaaagggc ctgacagaga ctgagacttg atcagaacag
    12181 agaattccct cctcccccac ctgccaatgg taagctgaca agccttgagt tagaagtaaa
    12241 aatgttacag gagagagaca agagtatggg aagaagaccc tttctgaggg tcacaaaggg
    12301 aggacctaaa gctgagcagg gagcacacat ggaaggagaa aatccctctg gcaggccagc
    12361 ctgcaccctc agtccaaggt gtcattggag gaactggaag ctgctgcatt gggggtaacc
    12421 atagcaacaa taaacctcaa acctagccca actctttttt ttatttactt tttagagaca
    12481 aggtcttgct ctgttgccca ggctgaagtg cagtggtgtg atcgcagctc actgcagcct
    12541 caaactcctg ggctcaagca atcctcctgc ttcagccttt gtaggagatt ggtcagggtg
    12601 gtgggagaaa ttataggaaa gacacaaacc ttcttggaag gccgagaggt tttgcaaaag
    12661 cttcagaaag aaattatggc tgaaggcagc caaattctta tctgaagcct gagagcaaag
    12721 ggcagataac aggggagttg tataggaact tacctagata aatttgttta ttcctgtgtc
    12781 cagaaaccaa cctttgatca ttcacacaca ggactgctgt ctacttggga tgttgacaat
    12841 gtttattgcc cacaaattgt gtttgctcca agcctttgtc attaaatttg tgctaaataa
    12901 atgtgagggc caccagctta aggggactgc taactctctt cggcccctag tgctggcagt
    12961 cccctagcct gctctctcac tgaa
//
