LOCUS       NM_001043229            2034 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens citrate synthase lysine methyltransferase (CSKMT),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001043229
VERSION     NM_001043229.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2034)
  AUTHORS   Malecki,J., Jakobsson,M.E., Ho,A.Y.Y., Moen,A., Rustan,A.C. and
            Falnes,P.O.
  TITLE     Uncovering human METTL12 as a mitochondrial methyltransferase that
            modulates citrate synthase activity through metabolite-sensitive
            lysine methylation
  JOURNAL   J Biol Chem 292 (43), 17950-17962 (2017)
   PUBMED   28887308
REFERENCE   4  (bases 1 to 2034)
  AUTHORS   Rhein,V.F., Carroll,J., Ding,S., Fearnley,I.M. and Walker,J.E.
  TITLE     Human METTL12 is a mitochondrial methyltransferase that modifies
            citrate synthase
  JOURNAL   FEBS Lett 591 (12), 1641-1652 (2017)
   PUBMED   28391595
  REMARK    GeneRIF: Data suggest that METTL12, localized in mitochondrial
            matrix, modifies/methylates Lys368 of citrate synthase in external
            surface region close to its catalytic site; addition/removal of
            methylation has no effect on citrate synthase activity; Lys368
            occurs in highly conserved sequence of amino acid residues of
            citrate synthase.
REFERENCE   5  (bases 1 to 2034)
  AUTHORS   Weber,M.J.
  TITLE     Mammalian small nucleolar RNAs are mobile genetic elements
  JOURNAL   PLoS Genet 2 (12), e205 (2006)
   PUBMED   17154719
  REMARK    Erratum:[PLoS Genet. 2007 Feb;3(2):e36]
REFERENCE   6  (bases 1 to 2034)
  AUTHORS   Taylor,T.D., Noguchi,H., Totoki,Y., Toyoda,A., Kuroki,Y., Dewar,K.,
            Lloyd,C., Itoh,T., Takeda,T., Kim,D.W., She,X., Barlow,K.F.,
            Bloom,T., Bruford,E., Chang,J.L., Cuomo,C.A., Eichler,E.,
            FitzGerald,M.G., Jaffe,D.B., LaButti,K., Nicol,R., Park,H.S.,
            Seaman,C., Sougnez,C., Yang,X., Zimmer,A.R., Zody,M.C.,
            Birren,B.W., Nusbaum,C., Fujiyama,A., Hattori,M., Rogers,J.,
            Lander,E.S. and Sakaki,Y.
  TITLE     Human chromosome 11 DNA sequence and analysis including novel gene
            identification
  JOURNAL   Nature 440 (7083), 497-500 (2006)
   PUBMED   16554811
REFERENCE   7  (bases 1 to 2034)
  AUTHORS   Choy,K.W., Wang,C.C., Ogura,A., Lau,T.K., Rogers,M.S., Ikeo,K.,
            Gojobori,T., Lam,D.S. and Pang,C.P.
  TITLE     Genomic annotation of 15,809 ESTs identified from pooled early
            gestation human eyes
  JOURNAL   Physiol Genomics 25 (1), 9-15 (2006)
   PUBMED   16368877
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP001458.6, BY796224.2, BX348415.2 and BG676615.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001043229.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.241705.1,
                                           SRR1803611.81617.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144335, SAMEA2145245
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000532971.2/
                                                  ENSP00000431287.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AP001458.6         72579-72599
            22-39               AP001458.6         72914-72931
            40-630              BY796224.2         1-591
            631-990             BX348415.2         203-562
            991-1377            BG676615.1         124-510
            1378-2034           AP001458.6         74719-75375
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.3"
     gene            1..2034
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /note="citrate synthase lysine methyltransferase"
                     /db_xref="GeneID:751071"
                     /db_xref="HGNC:HGNC:33113"
                     /db_xref="MIM:617897"
     exon            1..21
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /inference="alignment:Splign:2.1.0"
     exon            22..321
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /inference="alignment:Splign:2.1.0"
     CDS             255..977
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /EC_number="2.1.1.-"
                     /note="methyltransferase-like protein 12, mitochondrial;
                     methyltransferase like 12; citrate synthase-lysine
                     N-methyltransferase CSKMT, mitochondrial"
                     /codon_start=1
                     /product="citrate synthase-lysine N-methyltransferase
                     CSKMT, mitochondrial precursor"
                     /protein_id="NP_001036694.1"
                     /db_xref="CCDS:CCDS41657.1"
                     /db_xref="GeneID:751071"
                     /db_xref="HGNC:HGNC:33113"
                     /db_xref="MIM:617897"
                     /translation="MAALRRMLHLPSLMMGTCRPFAGSLADSCLADRCLWDRLHAQPR
                     LGTVPTFDWFFGYDEVQGLLLPLLQEAQAASPLRVLDVGCGTSSLCTGLYTKSPHPVD
                     VLGVDFSPVAVAHMNSLLEGGPGQTPLCPGHPASSLHFMHADAQNLGAVASSGSFQLL
                     LDKGTWDAVARGGLPRAYQLLSECLRVLNPQGTLIQFSDEDPDVRLPCLEQGSYGWTV
                     TVQELGPFRGITYFAYLIQGSH"
     exon            322..2034
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      973..978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /note="hexamer: ATTAAA"
     polyA_site      997
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /note="major polyA site"
     regulatory      2014..2019
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
                     /note="hexamer: ATTATA"
     polyA_site      2034
                     /gene="CSKMT"
                     /gene_synonym="CS-KMT; METTL12; U99HG"
ORIGIN      
        1 ctttcggagg gtggtgagct agtctgcgga cgctgtatac cctcctccac ttcccgctgc
       61 agccaataaa ggccgttcct accatagtct gtgtccgcgt tcttttttcc gggactgcag
      121 agttcgggga agctgtacgc cgcctttcgc tacgcggaat ttgcagatct tcccctggac
      181 ctcaggcctc tccggctgga gtagggtgga cgcttcacat aagcttctct ggtcgaactt
      241 acccgaatct ccagatggcc gcgctgcgtc gaatgctcca cttgccgagc ctgatgatgg
      301 ggacgtgccg cccctttgcg ggctcactgg ctgatagttg cctggcggac cgctgtctct
      361 gggatcggct gcatgcccag cctcgtttgg gcactgtccc caccttcgac tggttctttg
      421 gatacgacga agtccagggg ctcctactgc cattgctgca ggaggcacag gctgccagtc
      481 ctctgcgagt gctggatgtg ggctgtggga cttccagcct atgtacaggc ctctacacca
      541 aatctccaca cccagtggat gtgctggggg tggacttttc tcctgtggct gtggcccaca
      601 tgaatagcct cctggagggt ggcccaggcc aaacacctct atgccctgga caccctgcct
      661 caagcctcca cttcatgcac gccgatgctc agaacctggg ggctgtggct tcttcaggct
      721 ctttccaact actgctggac aaaggcacat gggatgctgt tgcccgggga ggtctgccta
      781 gggcttacca gcttctatca gaatgcttga gggttctaaa ccctcagggg accctgattc
      841 agttctcaga tgaggaccct gatgtgcgac tgccctgcct ggaacaaggg tcctatggct
      901 ggactgtgac tgtgcaggag ctaggcccgt tcaggggcat cacctacttt gcttacttga
      961 ttcaaggctc tcattaaaga cattttagta gtcctgaccc tagtatttct gtgggcaagg
     1021 agagggctga agaactgtct ttgcaagcta tctggctgca aagtgagaat ttgagtcctg
     1081 gcttccacat ttactagctg ggtgccatat tgctgaatgt ttctgttccc cagtttactc
     1141 atctgcagag tgagaataac ttggagttac ggagattaca tacaatgatg tgcgcaatat
     1201 ttagcacaaa atgaatgctg aaaagagaag gtacaattgg gtcattcccc agtttcaact
     1261 aactggagct cctaaaagca gcagacagga actgaatcaa aacccctgcg ctgactgact
     1321 tgtataatct agtggcctaa cctgtaagcc tcatttttgt cacctgtaaa aggagattgt
     1381 aagaggatgg gtataaggag cttcataaac ctggatgaga tatttgaggg ggagggaaca
     1441 atacttaccc tcaaagctat taggaggcag gagatgggaa tattccacaa agccacttct
     1501 acaaacacgg gagcctgttg agtcccagaa gggacagtgg ttggtggctt ccagacatgc
     1561 tgtgtcctca agaatccaac aagcatcttc ttcatcctgg tcctgccccc agctgagtcc
     1621 agcgttaaat gttcttaaag gacttctagg gtcctgtggg ctccaagccc agcctgggat
     1681 ggaggaagag aggggacaaa aatattactg atatattatc aaattacaaa actaggccag
     1741 gcatgctggc tcatacctat aatcacagca ctttgggagg ccaaggtggg cagattgctt
     1801 gagccctggc aataaagtga cacccctaac tctacaaaaa caaatgagcc cgggatagtg
     1861 gcacaggcat gtagttccaa ctactcagga ggctgagctg ggaggactgc ttgaaccctg
     1921 ggaggtcaaa gctgcaatgg gccatgataa gctgctgcac ttgagctaaa aattaatttc
     1981 tgtggtattt tttgcttata aaaaaataga gcaattatat cactaagagc ctaa
//
