LOCUS       NM_004383               2743 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens C-terminal Src kinase (CSK), transcript variant 1,
            mRNA.
ACCESSION   NM_004383
VERSION     NM_004383.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Brian,B.F. 4th, Sjaastad,F.V. and Freedman,T.S.
  TITLE     SH3-domain mutations selectively disrupt Csk homodimerization or
            PTPN22 binding
  JOURNAL   Sci Rep 12 (1), 5875 (2022)
   PUBMED   35393453
  REMARK    GeneRIF: SH3-domain mutations selectively disrupt Csk
            homodimerization or PTPN22 binding.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Chiva-Blanch,G., Pena,E., Cubedo,J., Garcia-Arguinzonis,M.,
            Pane,A., Gil,P.A., Perez,A., Ortega,E., Padro,T. and Badimon,L.
  TITLE     Molecular mapping of platelet hyperreactivity in diabetes: the
            stress proteins complex HSPA8/Hsp90/CSK2alpha and platelet
            aggregation in diabetic and normal platelets
  JOURNAL   Transl Res 235, 1-14 (2021)
   PUBMED   33887528
  REMARK    GeneRIF: Molecular mapping of platelet hyperreactivity in diabetes:
            the stress proteins complex HSPA8/Hsp90/CSK2alpha and platelet
            aggregation in diabetic and normal platelets.
REFERENCE   3  (bases 1 to 2743)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2743)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 2743)
  AUTHORS   Yao,Q., Liu,B.Q., Li,H., McGarrigle,D., Xing,B.W., Zhou,M.T.,
            Wang,Z., Zhang,J.J., Huang,X.Y. and Guo,L.
  TITLE     C-terminal Src kinase (Csk)-mediated phosphorylation of eukaryotic
            elongation factor 2 (eEF2) promotes proteolytic cleavage and
            nuclear translocation of eEF2
  JOURNAL   J Biol Chem 289 (18), 12666-12678 (2014)
   PUBMED   24648518
  REMARK    GeneRIF: Data indicate that the accumulation of the cleaved
            C-terminal small fragment of eukaryotic elongation factor 2 (eEF2)
            in the nucleus, and C-terminal Src kinase (Csk) could enhance the
            proteolytic cleavage of eEF2.
REFERENCE   6  (bases 1 to 2743)
  AUTHORS   Manjarrez-Orduno,N., Marasco,E., Chung,S.A., Katz,M.S.,
            Kiridly,J.F., Simpfendorfer,K.R., Freudenberg,J., Ballard,D.H.,
            Nashi,E., Hopkins,T.J., Cunninghame Graham,D.S., Lee,A.T.,
            Coenen,M.J., Franke,B., Swinkels,D.W., Graham,R.R., Kimberly,R.P.,
            Gaffney,P.M., Vyse,T.J., Behrens,T.W., Criswell,L.A., Diamond,B.
            and Gregersen,P.K.
  TITLE     CSK regulatory polymorphism is associated with systemic lupus
            erythematosus and influences B-cell signaling and activation
  JOURNAL   Nat Genet 44 (11), 1227-1230 (2012)
   PUBMED   23042117
  REMARK    GeneRIF: This suggests that the Lyp-Csk complex increases
            susceptibility to lupus at multiple maturation and activation
            points in B cells.
REFERENCE   7  (bases 1 to 2743)
  AUTHORS   Brauninger,A., Holtrich,U., Strebhardt,K. and Rubsamen-Waigmann,H.
  TITLE     Isolation and characterization of a human gene that encodes a new
            subclass of protein tyrosine kinases
  JOURNAL   Gene 110 (2), 205-211 (1992)
   PUBMED   1371489
REFERENCE   8  (bases 1 to 2743)
  AUTHORS   Armstrong,E., Cannizzaro,L., Bergman,M., Huebner,K. and Alitalo,K.
  TITLE     The c-src tyrosine kinase (CSK) gene, a potential antioncogene,
            localizes to human chromosome region 15q23----q25
  JOURNAL   Cytogenet Cell Genet 60 (2), 119-120 (1992)
   PUBMED   1377109
REFERENCE   9  (bases 1 to 2743)
  AUTHORS   Holtrich,U., Brauninger,A., Strebhardt,K. and Rubsamen-Waigmann,H.
  TITLE     Two additional protein-tyrosine kinases expressed in human lung:
            fourth member of the fibroblast growth factor receptor family and
            an intracellular protein-tyrosine kinase
  JOURNAL   Proc Natl Acad Sci U S A 88 (23), 10411-10415 (1991)
   PUBMED   1720539
REFERENCE   10 (bases 1 to 2743)
  AUTHORS   Partanen,J., Armstrong,E., Bergman,M., Makela,T.P., Hirvonen,H.,
            Huebner,K. and Alitalo,K.
  TITLE     cyl encodes a putative cytoplasmic tyrosine kinase lacking the
            conserved tyrosine autophosphorylation site (Y416src)
  JOURNAL   Oncogene 6 (11), 2013-2018 (1991)
   PUBMED   1945408
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC091230.23.
            
            On Sep 23, 2020 this sequence version replaced NM_004383.2.
            
            Summary: The protein encoded by this gene is involved in multiple
            pathways, including the regulation of Src family kinases. It plays
            an important role in T-cell activation through its association with
            the protein encoded by the protein tyrosine phosphatase,
            non-receptor type 22 (PTPN22) gene. This protein also
            phosphorylates C-terminal tyrosine residues on multiple substrates,
            including the protein encoded by the SRC proto-oncogene,
            non-receptor tyrosine kinase gene. Phosphorylation suppresses the
            kinase activity of the Src family tyrosine kinases. An intronic
            polymorphism (rs34933034) in this gene has been found to affect
            B-cell activation and is associated with systemic lupus
            erythematosus (SLE). Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Aug 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X59932.1, BC106073.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220003.14/ ENSP00000220003.9
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-641               AC091230.23        91247-91887         c
            642-721             AC091230.23        75655-75734         c
            722-835             AC091230.23        75239-75352         c
            836-948             AC091230.23        75029-75141         c
            949-1168            AC091230.23        74476-74695         c
            1169-1262           AC091230.23        73462-73555         c
            1263-1328           AC091230.23        73221-73286         c
            1329-1428           AC091230.23        73045-73144         c
            1429-1519           AC091230.23        72865-72955         c
            1520-1593           AC091230.23        72372-72445         c
            1594-1789           AC091230.23        72077-72272         c
            1790-1876           AC091230.23        71884-71970         c
            1877-2743           AC091230.23        70770-71636         c
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q24.1"
     gene            1..2743
                     /gene="CSK"
                     /note="C-terminal Src kinase"
                     /db_xref="GeneID:1445"
                     /db_xref="HGNC:HGNC:2444"
                     /db_xref="MIM:124095"
     exon            1..641
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            642..721
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     CDS             707..2059
                     /gene="CSK"
                     /EC_number="2.7.10.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tyrosine-protein kinase CSK; C-Src kinase;
                     protein-tyrosine kinase CYL; c-src tyrosine kinase; CSK,
                     non-receptor tyrosine kinase"
                     /codon_start=1
                     /product="tyrosine-protein kinase CSK isoform 1"
                     /protein_id="NP_004374.1"
                     /db_xref="CCDS:CCDS10269.1"
                     /db_xref="GeneID:1445"
                     /db_xref="HGNC:HGNC:2444"
                     /db_xref="MIM:124095"
                     /translation="MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKD
                     PNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETG
                     LFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDA
                     DGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNK
                     VAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVD
                     YLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFG
                     LTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPL
                     KDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL
                     "
     exon            722..835
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            836..948
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1168
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1262
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1328
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1428
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1519
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1593
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1789
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1876
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..2743
                     /gene="CSK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2414..2419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSK"
                     /note="hexamer: AAGAAA"
     polyA_site      2448
                     /gene="CSK"
                     /note="major polyA site"
     regulatory      2691..2696
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSK"
                     /note="hexamer: AATAAA"
     polyA_site      2743
                     /gene="CSK"
                     /note="major polyA site"
ORIGIN      
        1 ggatccgggc cgcgcttcct ctcgccaggc ctgcgagctt cctcccagcg gagccctggg
       61 cgagccgagg ttggccgccg ccgccgccga gcccgctgcc gccctcccgc tcctgcccca
      121 cccgcgcctt gcccgggggc ttctgccggg gtggggtccg agccgggcga ccgcccggct
      181 gcgccgccgt cggggccgta acccggcccg ccgtccctcc cgccccagcc agcctctggc
      241 cgccggagcc cgcggggcgt ggagcgcgag gagccccgcg gccccgatcg agcgtccggg
      301 gcggcccccg gcagccagcg cgacgttcca aaatcgaacc tcagtggcgg cgctcggaag
      361 cggaactctg ccggggccgc gccggctaca ttgtttcctc cccccgactc cctcccgccc
      421 ccttcccccg cctttcttcc ctccgcgacc cgggccgtgc gtccgtcccc ctgcctctgc
      481 ctggcggtcc ctcctcccct ctccttgcac ccatacctct ttgtaccgca ccccctgggg
      541 acccctgcgc ccctcccctc ccccctgacc gcatggaccg tcccgcaggc cgctgatgcc
      601 gcccgcggcg aggtggcccg gaccgcagtg ccccaagaga gctctaatgg taccaagtga
      661 caggttggct ttactgtgac tcggggacgc cagagctcct gagaagatgt cagcaataca
      721 ggccgcctgg ccatccggta cagaatgtat tgccaagtac aacttccacg gcactgccga
      781 gcaggacctg cccttctgca aaggagacgt gctcaccatt gtggccgtca ccaaggaccc
      841 caactggtac aaagccaaaa acaaggtggg ccgtgagggc atcatcccag ccaactacgt
      901 ccagaagcgg gagggcgtga aggcgggtac caaactcagc ctcatgcctt ggttccacgg
      961 caagatcaca cgggagcagg ctgagcggct tctgtacccg ccggagacag gcctgttcct
     1021 ggtgcgggag agcaccaact accccggaga ctacacgctg tgcgtgagct gcgacggcaa
     1081 ggtggagcac taccgcatca tgtaccatgc cagcaagctc agcatcgacg aggaggtgta
     1141 ctttgagaac ctcatgcagc tggtggagca ctacacctca gacgcagatg gactctgtac
     1201 gcgcctcatt aaaccaaagg tcatggaggg cacagtggcg gcccaggatg agttctaccg
     1261 cagcggctgg gccctgaaca tgaaggagct gaagctgctg cagaccatcg ggaaggggga
     1321 gttcggagac gtgatgctgg gcgattaccg agggaacaaa gtcgccgtca agtgcattaa
     1381 gaacgacgcc actgcccagg ccttcctggc tgaagcctca gtcatgacgc aactgcggca
     1441 tagcaacctg gtgcagctcc tgggcgtgat cgtggaggag aagggcgggc tctacatcgt
     1501 cactgagtac atggccaagg ggagccttgt ggactacctg cggtctaggg gtcggtcagt
     1561 gctgggcgga gactgtctcc tcaagttctc gctagatgtc tgcgaggcca tggaatacct
     1621 ggagggcaac aatttcgtgc atcgagacct ggctgcccgc aatgtgctgg tgtctgagga
     1681 caacgtggcc aaggtcagcg actttggtct caccaaggag gcgtccagca cccaggacac
     1741 gggcaagctg ccagtcaagt ggacagcccc tgaggccctg agagagaaga aattctccac
     1801 taagtctgac gtgtggagtt tcggaatcct tctctgggaa atctactcct ttgggcgagt
     1861 gccttatcca agaattcccc tgaaggacgt cgtccctcgg gtggagaagg gctacaagat
     1921 ggatgccccc gacggctgcc cgcccgcagt ctatgaagtc atgaagaact gctggcacct
     1981 ggacgccgcc atgcggccct ccttcctaca gctccgagag cagcttgagc acatcaaaac
     2041 ccacgagctg cacctgtgac ggctggcctc cgcctgggtc atgggcctgt ggggactgaa
     2101 cctggaagat catggacctg gtgcccctgc tcactgggcc cgagcctgaa ctgagcccca
     2161 gcgggctggc gggccttttt cctgcgtccc agcctgcacc cctccggccc cgtctctctt
     2221 ggacccacct gtggggcctg gggagcccac tgaggggcca gggaggaagg aggccacgga
     2281 gcgggaggca gcgccccacc acgtcgggct tccctggcct cccgccactc gccttcttag
     2341 agttttattc ctttcctttt ttgagatttt ttttccgtgt gtttattttt tattattttt
     2401 caagataagg agaaagaaag tacccagcaa atgggcattt tacaagaagt acgaatctta
     2461 tttttcctgt cctgcccgtg agggtggggg ggaccgggcc cctctctagg gacccctcgc
     2521 cccagcctca ttccccattc tgtgtcccat gtcccgtgtc tcctcggtcg ccccgtgttt
     2581 gcgcttgacc atgttgcact gtttgcatgc gcccgaggca gacgtctgtc aggggcttgg
     2641 atttcgtgtg ccgctgccac ccgcccaccc gccttgtgag atggaattgt aataaaccac
     2701 gccatgagga caccgccgcc cgcctcggcg cttcctccac cga
//
