LOCUS       NM_018590               3765 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens chondroitin sulfate N-acetylgalactosaminyltransferase
            2 (CSGALNACT2), transcript variant 1, mRNA.
ACCESSION   NM_018590
VERSION     NM_018590.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,M., Wang,C., Wu,M., Gu,S., Yang,J., Zhang,Y., Cheng,S., Xu,S.,
            Zhang,M., Wu,Y., Zhao,Y., Tian,X., Voon,D.C., Takahashi,C.,
            Sheng,J. and Wang,Y.
  TITLE     CSGALNACT2 restricts ovarian cancer migration and invasion by
            modulating MAPK/ERK pathway through DUSP1
  JOURNAL   Cell Oncol (Dordr) 47 (3), 897-915 (2024)
   PUBMED   38082211
  REMARK    GeneRIF: CSGALNACT2 restricts ovarian cancer migration and invasion
            by modulating MAPK/ERK pathway through DUSP1.
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3765)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3765)
  AUTHORS   Adhikara,I.M., Yagi,K., Mayasari,D.S., Ikeda,K., Kitagawa,H.,
            Miyata,O., Igarashi,M., Hatakeyama,K., Asada,Y., Hirata,K.I. and
            Emoto,N.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-2 deletion
            alleviates lipoprotein retention in early atherosclerosis and
            attenuates aortic smooth muscle cell migration
  JOURNAL   Biochem Biophys Res Commun 509 (1), 89-95 (2019)
   PUBMED   30579596
  REMARK    GeneRIF: Chondroitin sulfate N-acetylgalactosaminyltransferase 2 is
            functionally involved in the progression of atherosclerosis both in
            its early and advanced stages.
REFERENCE   5  (bases 1 to 3765)
  AUTHORS   Bret,C., Hose,D., Reme,T., Sprynski,A.C., Mahtouk,K., Schved,J.F.,
            Quittet,P., Rossi,J.F., Goldschmidt,H. and Klein,B.
  TITLE     Expression of genes encoding for proteins involved in heparan
            sulphate and chondroitin sulphate chain synthesis and modification
            in normal and malignant plasma cells
  JOURNAL   Br J Haematol 145 (3), 350-368 (2009)
   PUBMED   19298595
REFERENCE   6  (bases 1 to 3765)
  AUTHORS   Stroud,W.J., Jiang,S., Jack,G. and Storrie,B.
  TITLE     Persistence of Golgi matrix distribution exhibits the same
            dependence on Sar1p activity as a Golgi glycosyltransferase
  JOURNAL   Traffic 4 (9), 631-641 (2003)
   PUBMED   12911817
REFERENCE   7  (bases 1 to 3765)
  AUTHORS   Sato,T., Gotoh,M., Kiyohara,K., Akashima,T., Iwasaki,H.,
            Kameyama,A., Mochizuki,H., Yada,T., Inaba,N., Togayachi,A.,
            Kudo,T., Asada,M., Watanabe,H., Imamura,T., Kimata,K. and
            Narimatsu,H.
  TITLE     Differential roles of two N-acetylgalactosaminyltransferases,
            CSGalNAcT-1, and a novel enzyme, CSGalNAcT-2. Initiation and
            elongation in synthesis of chondroitin sulfate
  JOURNAL   J Biol Chem 278 (5), 3063-3071 (2003)
   PUBMED   12446672
  REMARK    GeneRIF: plays a role in the initiation and elongation in the
            synthesis of chondroitin sulfate
REFERENCE   8  (bases 1 to 3765)
  AUTHORS   Uyama,T., Kitagawa,H., Tanaka,J., Tamura,J., Ogawa,T. and
            Sugahara,K.
  TITLE     Molecular cloning and expression of a second chondroitin
            N-acetylgalactosaminyltransferase involved in the initiation and
            elongation of chondroitin/dermatan sulfate
  JOURNAL   J Biol Chem 278 (5), 3072-3078 (2003)
   PUBMED   12433924
REFERENCE   9  (bases 1 to 3765)
  AUTHORS   Uyama,T., Kitagawa,H., Tamura Ji,J. and Sugahara,K.
  TITLE     Molecular cloning and expression of human chondroitin
            N-acetylgalactosaminyltransferase: the key enzyme for chain
            initiation and elongation of chondroitin/dermatan sulfate on the
            protein linkage region tetrasaccharide shared by heparin/heparan
            sulfate
  JOURNAL   J Biol Chem 277 (11), 8841-8846 (2002)
   PUBMED   11788602
REFERENCE   10 (bases 1 to 3765)
  AUTHORS   Miles,S., McManus,H., Forsten,K.E. and Storrie,B.
  TITLE     Evidence that the entire Golgi apparatus cycles in interphase HeLa
            cells: sensitivity of Golgi matrix proteins to an ER exit block
  JOURNAL   J Cell Biol 155 (4), 543-555 (2001)
   PUBMED   11696556
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB291068.1, DA674683.1,
            BC030268.1 and BX102005.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018590.4.
            
            Summary: This gene encodes a member of the chondroitin
            N-acetylgalactosaminyltransferase family. The encoded protein is
            involved in elongation during chondroitin sulfate synthesis.
            Alternative splicing of this gene results in multiple transcript
            variants. Two related pseudogenes have been identified on
            chromosome X. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC030268.1, SRR1660805.163279.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374466.4/ ENSP00000363590.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                DB291068.1         1-27
            28-41               DA674683.1         23-36
            42-2814             BC030268.1         1-2773
            2815-3448           BX102005.1         87-720
            3449-3765           BC030268.1         3408-3724
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.21"
     gene            1..3765
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /note="chondroitin sulfate
                     N-acetylgalactosaminyltransferase 2"
                     /db_xref="GeneID:55454"
                     /db_xref="HGNC:HGNC:24292"
                     /db_xref="MIM:616616"
     exon            1..123
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     exon            124..1037
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..2005
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /EC_number="2.4.1.174"
                     /note="isoform 1 is encoded by transcript variant 1;
                     chondroitin beta1,4 N-acetylgalactosaminyltransferase 2;
                     beta 4 GalNAcT-2; glucuronylgalactosylproteoglycan
                     4-beta-N-acetylgalactosaminyltransferase 2"
                     /codon_start=1
                     /product="chondroitin sulfate
                     N-acetylgalactosaminyltransferase 2 isoform 1"
                     /protein_id="NP_061060.3"
                     /db_xref="CCDS:CCDS7201.1"
                     /db_xref="GeneID:55454"
                     /db_xref="HGNC:HGNC:24292"
                     /db_xref="MIM:616616"
                     /translation="MPRRGLILHTRTHWLLLGLALLCSLVLFMYLLECAPQTDGNASL
                     PGVVGENYGKEYYQALLQEQEEHYQTRATSLKRQIAQLKQELQEMSEKMRSLQERRNV
                     GANGIGYQSNKEQAPSDLLEFLHSQIDKAEVSIGAKLPSEYGVIPFESFTLMKVFQLE
                     MGLTRHPEEKPVRKDKRDELVEVIEAGLEVINNPDEDDEQEDEEGPLGEKLIFNENDF
                     VEGYYRTERDKGTQYELFFKKADLTEYRHVTLFRPFGPLMKVKSEMIDITRSIINIIV
                     PLAERTEAFVQFMQNFRDVCIHQDKKIHLTVVYFGKEGLSKVKSILESVTSESNFHNY
                     TLVSLNEEFNRGRGLNVGARAWDKGEVLMFFCDVDIYFSAEFLNSCRLNAEPGKKVFY
                     PVVFSLYNPAIVYANQEVPPPVEQQLVHKKDSGFWRDFGFGMTCQYRSDFLTIGGFDM
                     EVKGWGGEDVHLYRKYLHGDLIVIRTPVPGLFHLWHEKRCADELTPEQYRMCIQSKAM
                     NEASHSHLGMLVFREEIETHLHKQAYRTNSEAVG"
     exon            1038..1254
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1356
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1535
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1630
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1712
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..3765
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3737..3742
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /note="hexamer: AATAAA"
     polyA_site      3765
                     /gene="CSGALNACT2"
                     /gene_synonym="beta4GalNAcT; ChGn-2; CHGN2; GALNACT-2;
                     GALNACT2; PRO0082"
                     /note="major polyA site"
ORIGIN      
        1 ctctcaggcg cggcggcgcg cccgggggtg ggtggctgag gcggcggcgg gcccaaggcg
       61 tgaggcgccg cccgggtgtc cccgcggcgc aggaggcggt ggagcgcaga gcgggcgagc
      121 gcgaaaaatc actaccaata taatggattt tatatatcag attgctttat tctggatatc
      181 atggtaacaa tacagaaagt atacataatt tcccatttct gcaagtagtc atgactgctg
      241 aagaaagaaa aacttaaagc tacggcagaa ttattttatg gaaattctga ttttgttttt
      301 aatttttgat aactttttac taaaggtatg aacacacaaa gagcttattt tgttaggcaa
      361 atacacatta ataagaatgc ctagaagagg actgattctt cacacccgga cccactggtt
      421 gctgttgggc cttgctttgc tctgcagttt ggtattattt atgtacctcc tggaatgtgc
      481 cccccagact gatggaaatg catctcttcc tggtgttgtt ggggaaaatt atggtaaaga
      541 gtattatcaa gccctcctac aggaacaaga agaacattat cagaccaggg caaccagtct
      601 gaaacgccaa attgcccaac taaaacaaga attacaagaa atgagtgaga agatgcggtc
      661 actgcaagaa agaaggaatg taggggctaa tggcataggc tatcagagca acaaagagca
      721 agcacctagt gatcttttag agtttcttca ttcccaaatt gacaaagctg aagttagcat
      781 aggggccaaa ctacccagtg agtatggggt cattcccttt gaaagtttta ccttaatgaa
      841 agtatttcaa ttggaaatgg gtctcactcg ccatcctgaa gaaaagccag ttagaaaaga
      901 caaacgagat gaattggtgg aagttattga agcgggcttg gaggtcatta ataatcctga
      961 tgaagatgat gaacaagaag atgaggaggg tccccttgga gagaaactga tatttaatga
     1021 aaatgacttc gtagaaggtt attatcgcac tgagagagat aagggcacac agtatgaact
     1081 cttttttaag aaagcagacc ttacggaata tagacatgtg accctcttcc gcccttttgg
     1141 acctctcatg aaagtgaaga gtgagatgat tgacatcact agatcaatta ttaatatcat
     1201 tgtgccactt gctgaaagaa ctgaagcatt tgtacaattt atgcagaact tcagggatgt
     1261 ttgtattcat caagacaaga agattcatct cacagtggtg tattttggta aagaaggact
     1321 gtctaaagtc aagtctatcc tagaatctgt caccagtgag tctaattttc acaattacac
     1381 cttggtctca ttgaatgaag aatttaatcg tggacgagga ctaaatgtgg gtgcccgagc
     1441 ttgggacaag ggagaggtct tgatgttttt ctgtgatgtt gatatctatt tctcagccga
     1501 attccttaac agctgccggt taaatgctga gccaggtaag aaggtgtttt accctgtggt
     1561 gttcagtctt tacaatcctg ccattgttta tgccaaccag gaagtgccac cacctgtgga
     1621 gcagcagctg gttcacaaaa aggattctgg cttttggcga gattttggct ttggaatgac
     1681 ttgtcagtat cgttcagatt tcctgaccat tggtggattt gacatggaag tgaaaggttg
     1741 gggtggagaa gatgttcatc tttatcgaaa atacttacat ggtgacctca ttgtgattcg
     1801 gactccggtt cctggtcttt tccacctctg gcatgaaaag cgctgtgctg atgagctgac
     1861 ccccgagcag taccgcatgt gcatccagtc taaagccatg aatgaggcct ctcactccca
     1921 cctgggaatg ctggtcttca gggaggaaat agagacgcat cttcataaac aggcatacag
     1981 gacaaacagt gaagctgttg gttgaaatca taattaatgc gttactgtat gaaccacaaa
     2041 acagcactat ttatttagcc ttacttctac ttccagatgc agtgcctctt ttggagaaga
     2101 catgtttatt tttcatgttc tttctgacat tactttagca attcaacttg atgtgagaag
     2161 aaaaaacaaa tgtttcaaca caaaatctct gttttgtgag aatactgcac tatggaataa
     2221 ttgacaaatt gaaatctcat atttgtccca aaagttgttt tgagttagtt ctacctggtg
     2281 cccatgttct gattgtgtgt gggattgcat ggtgtcctga ttgcatctag gtggagcgga
     2341 tggaatgtgc tgggccactg ttgggtggag agcagcacat tcttacagag gagatggagc
     2401 gttatgagca tagtatgtgg ataggtatct tcacctgccc gcccctgagt cagcctcctt
     2461 gacttgatag cttgaagaat ccttttccac tgaaatagag gataattaat tgacacatct
     2521 gaaatcccca atcaatcaat caagagaaag gtagaactaa aaactcctta acttactgtt
     2581 gcttacaccc ctgaaagtct gtttttaagc aaatgggtaa tagtagaaaa taggttagaa
     2641 tctatggctt gattaaaaat atgttattac attatcatgt tcaggattag gattagtagt
     2701 cagttgctgt aaactatttt gaacaaacag aaaagaacac ggaaacattt ttaacagagc
     2761 atttaattat gttggaatac aggatcctag ctctgtctgg gaacattagt ttatttgagc
     2821 cagctctatc agggtcttcc catggtggtt cagaatagat gagcatagca tggttttgtt
     2881 tgtttttgct ttcaattttc taatttggca tggatccata tgtatttact atcctttttc
     2941 taatatatta atatatgcta catttgtatt tgcattacta taatactttg agttgaaaaa
     3001 gagtttcatt gtggagagaa aaagcaaatg gtatgccaca agatcactct gatttgagaa
     3061 aagggaggag gggaagatag tctgaatgga aatctgaaat acggaatgtt ttagagaaat
     3121 atgtcacttg catatagaat gttttaattg aggtataaat taatgagaca aagtgaaaaa
     3181 gaaattatat tcagatagga ctgcactaca ttatttgtca cacatggatc tgttaccatc
     3241 aggtcaattc ctagtatgca taaatttttt aaccctttta aaagagacct atgttgaaaa
     3301 cccctgaaaa ttcactgaag aaaaatcatt actctttttc tcagtaaatc atatcatctg
     3361 aaatattaca aatttcaaat ttctaggtgc tatattaatt caatattaca ataactctta
     3421 cctaattatt cttacaagtt ttaagttgtg gtagtttagt gattttttta aaagatgtgt
     3481 gaaatgttct ctgcaaaata attcaggcca ctgtctcctt ttatatatta ttataattat
     3541 ttattatgaa gaccagtgaa ttacgatatt taaagtgaga gaacttaatt atttgcaaag
     3601 gtaagttaca gcttgttttt tgagagaatc aaatgagttt acttttgttc ctgttgtttt
     3661 taactagctt taagtttaaa gatggaagct aagcaatgga aatgctatac gtttttgaca
     3721 tttattaaat ggtaccaata aagtatttta ttaccaaaag ttaaa
//
