LOCUS       NM_001371242           10039 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens crystallin beta-gamma domain containing 1 (CRYBG1),
            transcript variant 1, mRNA.
ACCESSION   NM_001371242 XM_005266839
VERSION     NM_001371242.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Scholl,Z.N., Li,Q., Yang,W. and Marszalek,P.E.
  TITLE     Single-molecule Force Spectroscopy Reveals the Calcium Dependence
            of the Alternative Conformations in the Native State of a
            betagamma-Crystallin Protein
  JOURNAL   J Biol Chem 291 (35), 18263-18275 (2016)
   PUBMED   27378818
  REMARK    GeneRIF: Single-molecule Force Spectroscopy Reveals the Calcium
            Dependence of the Alternative Conformations in the Native State of
            a betagamma-Crystallin Protein.
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   Das,R., Lee,Y.K., Strogantsev,R., Jin,S., Lim,Y.C., Ng,P.Y.,
            Lin,X.M., Chng,K., Yeo,G.Sh., Ferguson-Smith,A.C. and Ding,C.
  TITLE     DNMT1 and AIM1 Imprinting in human placenta revealed through a
            genome-wide screen for allele-specific DNA methylation
  JOURNAL   BMC Genomics 14, 685 (2013)
   PUBMED   24094292
  REMARK    GeneRIF: Both DNMT1 and AIM1 show maternal allele-specific
            methylation and paternal allele-specific transcription.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10039)
  AUTHORS   Rosenbaum,E., Begum,S., Brait,M., Zahurak,M., Maldonado,L.,
            Mangold,L.A., Eisenberger,M.A., Epstein,J.I., Partin,A.W.,
            Sidransky,D. and Hoque,M.O.
  TITLE     AIM1 promoter hypermethylation as a predictor of decreased risk of
            recurrence following radical prostatectomy
  JOURNAL   Prostate 72 (10), 1133-1139 (2012)
   PUBMED   22127895
  REMARK    GeneRIF: Methylation status of AIM1 in the prostate cancer specimen
            may predict for time to recurrence in Gleason 3 + 4 = 7 patients
            undergoing prostatectomy.
REFERENCE   4  (bases 1 to 10039)
  AUTHORS   Hoshimoto,S., Kuo,C.T., Chong,K.K., Takeshima,T.L., Takei,Y.,
            Li,M.W., Huang,S.K., Sim,M.S., Morton,D.L. and Hoon,D.S.
  TITLE     AIM1 and LINE-1 epigenetic aberrations in tumor and serum relate to
            melanoma progression and disease outcome
  JOURNAL   J Invest Dermatol 132 (6), 1689-1697 (2012)
   PUBMED   22402438
  REMARK    GeneRIF: AIM1 promoter hypermethylation was found in higher
            frequency (P = 0.005) in metastatic melanoma (65%) than in primary
            melanomas (38%).
REFERENCE   5  (bases 1 to 10039)
  AUTHORS   Vainio,P., Mpindi,J.P., Kohonen,P., Fey,V., Mirtti,T., Alanen,K.A.,
            Perala,M., Kallioniemi,O. and Iljin,K.
  TITLE     High-throughput transcriptomic and RNAi analysis identifies AIM1,
            ERGIC1, TMED3 and TPX2 as potential drug targets in prostate cancer
  JOURNAL   PLoS One 7 (6), e39801 (2012)
   PUBMED   22761906
  REMARK    GeneRIF: AIM1, ERGIC1, and TPX2 were shown to be highly expressed
            especially in prostate cancer tissues, and high mRNA expression of
            ERGIC1 and TMED3 associated with AR and ERG oncogene expression
REFERENCE   6  (bases 1 to 10039)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   7  (bases 1 to 10039)
  AUTHORS   Rajini,B., Graham,C., Wistow,G. and Sharma,Y.
  TITLE     Stability, homodimerization, and calcium-binding properties of a
            single, variant betagamma-crystallin domain of the protein absent
            in melanoma 1 (AIM1)
  JOURNAL   Biochemistry 42 (15), 4552-4559 (2003)
   PUBMED   12693952
REFERENCE   8  (bases 1 to 10039)
  AUTHORS   Ray,M.E., Wistow,G., Su,Y.A., Meltzer,P.S. and Trent,J.M.
  TITLE     AIM1, a novel non-lens member of the betagamma-crystallin
            superfamily, is associated with the control of tumorigenicity in
            human malignant melanoma
  JOURNAL   Proc Natl Acad Sci U S A 94 (7), 3229-3234 (1997)
   PUBMED   9096375
REFERENCE   9  (bases 1 to 10039)
  AUTHORS   Ray,M.E., Su,Y.A., Meltzer,P.S. and Trent,J.M.
  TITLE     Isolation and characterization of genes associated with
            chromosome-6 mediated tumor suppression in human malignant melanoma
  JOURNAL   Oncogene 12 (12), 2527-2533 (1996)
   PUBMED   8700511
REFERENCE   10 (bases 1 to 10039)
  AUTHORS   Millikin,D., Meese,E., Vogelstein,B., Witkowski,C. and Trent,J.
  TITLE     Loss of heterozygosity for loci on the long arm of chromosome 6 in
            human malignant melanoma
  JOURNAL   Cancer Res 51 (20), 5449-5453 (1991)
   PUBMED   1680551
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL109920.15, AL359292.12 and AL390074.17.
            
            On Aug 27, 2019 this sequence version replaced NM_001371242.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3722516.1,
                                           SRR11853567.26108.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000633556.3/ ENSP00000488010.2
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-365               AL109920.15        142586-142950       c
            366-504             AL109920.15        51835-51973         c
            505-2114            AL359292.12        44433-46042         c
            2115-4437           AL359292.12        36019-38341         c
            4438-4485           AL359292.12        32292-32339         c
            4486-4604           AL359292.12        32086-32204         c
            4605-4770           AL359292.12        30002-30167         c
            4771-4910           AL359292.12        27157-27296         c
            4911-5037           AL359292.12        17943-18069         c
            5038-5073           AL359292.12        15851-15886         c
            5074-5231           AL359292.12        13875-14032         c
            5232-5358           AL359292.12        12775-12901         c
            5359-5504           AL359292.12        12539-12684         c
            5505-5631           AL359292.12        5494-5620           c
            5632-5664           AL359292.12        5256-5288           c
            5665-5777           AL359292.12        3905-4017           c
            5778-5907           AL359292.12        1575-1704           c
            5908-6047           AL390074.17        136753-136892       c
            6048-6171           AL390074.17        134452-134575       c
            6172-6330           AL390074.17        133878-134036       c
            6331-6493           AL390074.17        131452-131614       c
            6494-10039          AL390074.17        123361-126906       c
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q21"
     gene            1..10039
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /note="crystallin beta-gamma domain containing 1"
                     /db_xref="GeneID:202"
                     /db_xref="HGNC:HGNC:356"
                     /db_xref="MIM:601797"
     exon            1..365
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..6588
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta-gamma crystallin domain containing 1; suppression of
                     tumorigenicity 4 (malignant melanoma); absent in melanoma
                     1 protein; beta/gamma crystallin domain-containing protein
                     1"
                     /codon_start=1
                     /product="beta/gamma crystallin domain-containing protein
                     1 isoform 1"
                     /protein_id="NP_001358171.1"
                     /db_xref="CCDS:CCDS93984.1"
                     /db_xref="GeneID:202"
                     /db_xref="HGNC:HGNC:356"
                     /db_xref="MIM:601797"
                     /translation="MPLSPPAQGDPGEPSPCRPPKKHTTFHLWRSKKKQQPAPPDCGV
                     FVPHPLPAPAGEARALDVVDGKYVVRDSQEFPLHCGESQFFHTTSEALGSLLLESGIF
                     KKSRAQPPEDNRRKPVLGKLGTLFTAGRRRNSRNGLESPTRSNAKPLSPKDVVASPKL
                     PERESERSRSQSSQLKQTDTSEEGSPRENPREAEGELPESGGPAAPPDAELSPRWSSS
                     AAAVAVQQCHENDSPQLEPLEAEGEPFPDATTTAKQLHSSPGNSSRQENAETPARSPG
                     EDASPGAGHEQEAFLGVRGAPGSPTQERPAGGLGEAPNGAPSVCAEEGSLGPRNARSQ
                     PPKGASDLPGEPPAEGAAHTASSAQADCTARPKGHAHPAKVLTLDIYLSKTEGAQVDE
                     PVVITPRAEDCGDWDDMEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDD
                     EVAPNAASDNASAEKKVKSPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPP
                     ASSPTKRKGRSRALEAVPAPPASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARA
                     IPRELPVKSSSLLPEIKPEHKRGPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKP
                     RNHFGVGRSTVTTKVTLPAKPKHVELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKIS
                     LFENKRTNSSPRHTDIRGQRNTPASSKTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQN
                     GVLVKETAIETKVTVSEEEILPATRGMNGDSSENQALGPQPNQDDKADVQTDAGCLSE
                     PVASALIPVKDHKLLEKEDSEAADSKSLVLENVTDTAQDIPTTVDTKDLPPTAMPKPQ
                     HTFSDSQSPAESSPGPSLSLSAPAPGDVPKDTCVQSPISSFPCTDLKVSENHKGCVLP
                     VSRQNNEKMPLLELGGETTPPLSTERSPEAVGSECPSRVLVQVRSFVLPVESTQDVSS
                     QVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEEVLGNEHSHCTAELAAKSGPQVI
                     PPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLATPQRPDQTVTNGQDSPASLL
                     NISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKARMPNSPAPHFAMPPIHED
                     HLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLRPKRASAEQSVLFKSLH
                     TNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLSSLPQDKIFSPSVTS
                     VNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNSLKVFNFNSSSTS
                     HSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDMEKANHIESVIK
                     SNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPRPGKVVIYS
                     EPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLEEGELEL
                     SGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSYFDDT
                     EEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSM
                     RPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPF
                     TSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSN
                     AHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQ
                     YILDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEE
                     TTSQIDDSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSL
                     RFIDVEFSEPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGIWVTYEY
                     GSYRGRQFLLSPAEVPNWYEFSGCRQIGSLRPFVQKRIYFRLRNKATGLFMSTNGNLE
                     DLKLLRIQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNA
                     DSQFWSLKSDGRIYSKLKPNLVLDIKGGTQYDQNHIILNTVSKEKFTQVWEAMVLYT"
     exon            366..504
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            505..2114
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..4437
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..4485
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            4486..4604
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            4605..4770
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            4771..4910
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            4911..5037
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5038..5073
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5074..5231
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5232..5358
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5359..5504
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5505..5631
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5632..5664
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5665..5777
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5778..5907
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            5908..6047
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            6048..6171
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            6172..6330
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            6331..6493
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     exon            6494..10039
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8448..8453
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /note="hexamer: AATAAA"
     polyA_site      8471
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /note="major polyA site"
     regulatory      9981..9986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /note="hexamer: AATAAG"
     regulatory      10000..10005
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
                     /note="hexamer: ACTAAA"
     polyA_site      10000
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
     polyA_site      10036
                     /gene="CRYBG1"
                     /gene_synonym="AIM1; ST4"
ORIGIN      
        1 agtgctgctc gcgctgcgct gggtgctggt tctgcaaccc gcggccgtcc ccccgcatcc
       61 gcgacgaggg ggcggggtcc cacggcgcgc tgagaaaggc gggcgagctg gcgctcaggt
      121 gtgttcttcc atagggcccg ggcggcagag aggaccgcgt cccggcagtc ggagcgggag
      181 gaggacaaga cgatgccgct gtccccgcca gcccagggcg accccgggga gcccagcccg
      241 tgcaggcccc ctaagaagca caccaccttc cacctctggc gctccaaaaa gaagcagcag
      301 ccggcgccgc ctgactgtgg ggtgttcgtt ccgcacccgc tcccggcgcc tgccggagag
      361 gccagagctt tggatgtagt cgatggaaaa tatgtggttc gagactccca ggaatttcca
      421 ctgcactgtg gggaatccca gttcttccac accaccagtg aggcgcttgg ttccttactt
      481 ctagagtctg gaatatttaa aaagtccaga gcacaacctc cagaagacaa cagaaggaag
      541 ccagttttgg ggaaacttgg cactctattc actgcaggaa ggagaagaaa cagtagaaac
      601 gggttagaga gtcccaccag atcaaatgcc aaaccactct ctcccaaaga tgtggtagcc
      661 tctcctaagc tcccagagag agagagtgag aggagcagat ctcagagcag ccaactgaag
      721 caaacggaca caagcgagga gggctccccg cgggagaatc cccgagaggc agagggcgag
      781 ctccccgaga gcggtggccc cgcagccccc cctgacgccg agctgtcacc tcgctggagc
      841 agcagtgcag cggctgtggc tgtgcagcag tgccatgaaa atgattcacc ccaattagaa
      901 cctctggagg cagagggaga gcctttccca gatgccacca ccactgccaa gcagctgcat
      961 tcctcgccgg gaaattcctc caggcaagag aacgcagaga cgcccgcccg cagtccgggg
     1021 gaggacgctt caccaggtgc tggccacgaa caggaggctt tcctgggtgt gaggggtgcg
     1081 ccagggtcgc ccacccagga gcggcccgcg ggaggactag gcgaggcccc taacggagcc
     1141 cccagtgtgt gtgccgaaga aggctccctg gggccccgca acgcccgcag ccagcccccc
     1201 aagggcgcgt ctgatttgcc aggtgagcct ccggccgagg gcgcagcgca cacggccagc
     1261 tccgcgcagg cagactgcac agcccgcccc aagggtcacg cccaccctgc taaggtgcta
     1321 actttggaca tctacttgag taagactgag ggggcacaag tggacgagcc ggtcgtgatt
     1381 actcccagag cggaagattg cggtgactgg gacgacatgg agaagaggtc cagcggccgt
     1441 aggtcgggga ggcggagggg gtcgcagaaa tccaccgact cccccggcgc ggacgccgag
     1501 ctccctgaga gcgctgccag ggacgacgcg gtgttcgacg acgaggtggc gccaaacgcg
     1561 gccagcgata acgcctcggc ggaaaagaaa gtgaaatctc cgcgggcagc cctcgacggg
     1621 ggcgttgcct ccgctgcgag cccagagtcc aagcccagcc ccggtaccaa agggcagctc
     1681 cgaggggagt cggaccggag caaacagcca cccccggctt cgtcccccac gaagaggaag
     1741 ggcaggagcc gtgccctcga ggccgtgccc gccccgcccg ccagcggccc ccgggctccc
     1801 gccaaggagt ccccacccaa gagggtgccc gatcccagcc cagtcaccaa gggcactgcg
     1861 gccgagagcg gggaggaggc ggcgcgggcc atcccccgcg agctcccggt caagagcagc
     1921 tcgctgctgc cggagatcaa gcccgagcac aagaggggcc cgctccccaa ccacttcaac
     1981 ggccgggcag agggaggtcg aagcagagag ctgggcagag cggccggagc gcctggagct
     2041 tctgacgccg acggcttgaa gcccaggaac catttcggcg tgggcaggtc gacagtgacc
     2101 actaaagtga ccctccctgc caagcccaaa catgtggaac taaatcttaa aacccctaag
     2161 aatcttgaca gtttgggaaa tgagcacaat ccatttagcc agccagttca caaaggcaac
     2221 actgccacca aaatctcctt atttgaaaac aaacggacaa acagtagccc aagacacact
     2281 gacattcgag gccaaaggaa tactcctgcc tctagtaaaa cgtttgttgg gagggcaaag
     2341 ctgaatttag ccaaaaaagc caaagaaatg gagcaacctg aaaagaaagt aatgccaaac
     2401 agtccccaga atggtgtgct ggttaaggaa actgctatag aaaccaaagt taccgtctcg
     2461 gaagaagaga ttctgccagc aaccagagga atgaatggag actcttctga gaatcaagct
     2521 cttggtcctc agcctaacca agatgataaa gcagatgtac aaacagatgc tggctgcctt
     2581 tcagaaccag tggcttctgc tctgattcct gtcaaggatc ataagctctt agagaaggag
     2641 gactcagagg ctgcagacag caaaagcctt gtacttgaaa atgtaaccga tacagcacaa
     2701 gacatcccca ccactgtgga taccaaagat ttacctccaa cggccatgcc aaagccacag
     2761 catacatttt ctgactcaca gtcccctgct gagtcatctc ctgggccttc tctttcactg
     2821 tctgcacccg ctcctgggga tgttcccaaa gacacatgtg ttcaatcacc cataagcagt
     2881 ttcccatgca ctgatctaaa agtgtcagaa aaccataaag gatgtgtttt gcctgtgtct
     2941 cgtcagaaca atgagaaaat gccactttta gaacttggag gagaaacaac ccctcctttg
     3001 tccacagagc gtagtccaga agctgtggga agtgagtgtc catccagagt cctcgtccag
     3061 gtcaggtcct tcgtgctccc cgtggagagc acccaggatg tgagctccca ggtcatccca
     3121 gagagctctg aagttagaga agtgcagttg ccaacttgtc acagtaatga acctgaagtg
     3181 gtttccgttg caagttgtgc tcccccacaa gaggaagtac tgggcaatga acactctcat
     3241 tgcacagcag agctcgcggc aaaatctggc ccacaagtca taccgccagc atcagagaaa
     3301 actctgccta ttcaggctca aagtcagggc agcagaacac ccctgatggc tgaatccagt
     3361 cccaccaact ctcccagcag cggaaatcac ttagccactc ctcaaaggcc agatcagact
     3421 gttacaaatg gccaggatag ccctgccagc cttttgaaca tttctgctgg tagtgatgat
     3481 agtgtatttg attcttcttc tgatatggaa aaattcactg aaattataaa acagatggat
     3541 agcgcagttt gtatgcccat gaaaagaaag aaggccagga tgccaaactc tcctgctcct
     3601 cactttgcca tgcctcctat tcacgaagac catttagaaa aggtgtttga tcccaaagtg
     3661 tttacctttg gtttggggaa gaagaaggaa agtcagccag aaatgtcacc ggctttacat
     3721 ttgatgcaga accttgacac aaaatccaaa ctgagaccca aacgtgcatc tgctgaacag
     3781 agcgtcctct tcaagtccct gcacaccaac actaatggga acagtgagcc tctggtgatg
     3841 ccggaaatca atgacaaaga gaacagggac gtcacaaatg gtggcattaa gagatcgaga
     3901 ctagaaaaaa gtgcactttt ctcaagcttg ttatcttctt taccacaaga caaaatcttt
     3961 tctccttctg tgacatcagt caacactatg accacggctt tcagtacttc tcagaacggt
     4021 tccctatctc agtcttcagt gtcacagccc acgactgagg gtgccccgcc ctgtggtttg
     4081 aacaaagaac agtcaaatct tctgcccgac aactccttaa aggtcttcaa tttcaactcg
     4141 tcaagtacat cacactccag tttgaaaagt ccaagccaca tggaaaaata cccgcaaaaa
     4201 gagaaaacca aagaagatct ggattcacga agcaacctac acttgccaga aactaaattt
     4261 tctgaattgt caaaactgaa gaatgatgat atggaaaagg ctaatcatat tgaaagtgtt
     4321 attaaatcaa acttgccaaa ctgtgcaaac agtgacaccg acttcatggg tcttttcaaa
     4381 tcaagccggt atgacccaag catttctttt tctggaatgt cattatcaga cacaatgaca
     4441 cttagaggaa gtgtccaaaa taaactcaat ccccgacctg gaaaggtagt gatatatagt
     4501 gaacccgacg tctctgagaa gtgcattgaa gttttcagtg acattcagga ttgcagttct
     4561 tggagcctct ctccagtgat actcataaaa gttgttagag gatgttggat tttgtatgag
     4621 caaccaaatt ttgaagggca ctccatcccc ttagaagaag gagaattgga actctctggt
     4681 ctctggggta tagaagacat tttggaaagg cacgaagaag cagagtctga taagccagtg
     4741 gtgattggtt ccatcagaca tgtggttcag gattacagag ttagtcacat tgacttattt
     4801 actgaaccag aagggttagg aatcctaagt tcctactttg atgatactga agaaatgcag
     4861 ggatttggtg taatgcagaa gacttgttcc atgaaagtac attggggcac gtggctgatt
     4921 tatgaagaac ctggatttca gggtgttcct ttcatcctgg aacctggtga ataccctgac
     4981 ttgtccttct gggatacaga agaagcgtac attggatcca tgcggcctct gaaaatgggt
     5041 ggccgtaaag ttgaattccc tacagatcca aaggtagttg tttatgaaaa gcctttcttt
     5101 gaaggaaaat gtgtggaact agaaacagga atgtgtagtt ttgtcatgga gggaggtgaa
     5161 acagaagagg cgactggaga cgatcatttg ccgtttacgt cagtggggtc tatgaaagtt
     5221 ctaagaggca tttgggttgc atatgagaaa cctggattta ccggtcatca gtatttgcta
     5281 gaagaaggag aatacaggga ctggaaagcc tggggaggtt acaatggaga gcttcagtct
     5341 ttacgaccta tattaggtga tttttcaaat gctcacatga taatgtacag tgaaaaaaac
     5401 tttggatcca aaggttccag tattgatgta ttgggaattg ttgctaattt aaaggagact
     5461 ggatatggag tgaagacaca gtctattaat gtactgagtg gagtatgggt agcctatgaa
     5521 aatcctgact tcacaggaga acagtatata ctggataaag gattttatac cagttttgag
     5581 gactggggag gcaaaaattg taagatctct tctgttcaac ctatatgttt ggattctttc
     5641 actggcccaa ggagacgaaa tcagattcac ttgttttcag aaccacagtt tcaaggtcac
     5701 agtcaaagtt ttgaagaaac aacaagtcaa attgatgatt cattttctac caagtcttgc
     5761 agagtttcag gaggcagctg ggttgtatat gatggagaaa atttcactgg taatcaatac
     5821 gtgttggaag aaggccatta tccttgtctg tctgcaatgg gatgcccgcc tggagcaact
     5881 ttcaagtctc ttcgttttat agatgttgaa ttttctgaac caacaattat tctctttgaa
     5941 agagaagact tcaaaggaaa aaagattgaa cttaatgcag aaactgtcaa tctccgatcc
     6001 ctgggattca acacacaaat acgctctgtt caggttattg gtggcatatg ggttacttat
     6061 gaatatggca gttacagagg gcgacagttc ctattgtcac ctgcagaagt acctaattgg
     6121 tatgaattca gtggctgtcg ccaaataggt tctctacgac cttttgttca gaagcgaatt
     6181 tatttcagac ttcgaaacaa agcaacaggg ttattcatgt caaccaatgg aaacttagag
     6241 gatctgaagc ttctgaggat acaggtcatg gaggatgtcg gggccgatga tcagatttgg
     6301 atctatcaag aaggatgtat caaatgcagg atagcagaag actgctgcct gacgattgtg
     6361 ggcagcctgg taacatctgg ctccaagcta ggcctggccc tggaccagaa tgctgacagc
     6421 cagttctgga gcttgaagtc cgatggcagg atttacagca agttgaagcc aaatttagtt
     6481 ttagacatta aagggggcac acagtatgat caaaatcaca ttatcctcaa cactgtcagc
     6541 aaagagaagt ttacacaagt gtgggaagcc atggtcctat atacctgaac aaagaaggaa
     6601 gaagaatctt ctggaggtcc ttccagccac cttatttctt aaaaaggaca atgctgatgg
     6661 aagaccagac tggaaagtgg atcgactcct ccttcattga ttctaaattc aaccttaaat
     6721 catgctgcca tgactcagag aacttactca tcgtttcaaa agactatcat agctttaaac
     6781 caataatttg tcctcctttc atttcttgcc tttcattttt ggtagctgct taaacaggtt
     6841 gcctaattag cagcttttgg gtgattttgt aaaatgttat atcaagattt caagactgtg
     6901 tacattttaa attatttcca aagatagtga caggagagaa ctggaacaaa tttaccaact
     6961 ttgtggacct acaaagccct tacactttaa agggtaagac aaaggcttaa gtttgaaagg
     7021 tagagaactg tttagcatct gagaagaaat actttattag gcctgtaatt ttggttcttg
     7081 gccttaaaca ctttctggaa cctttaaata tgctgcatag cacaatggga aagccttagg
     7141 tattcacaca tttaaggaac tctaaacaaa atactatttt cctttagttc atattaaaaa
     7201 ttaatacatt ttaaaaattt aatgtcaaag tctggtaaca tttgttagta ggatttgagt
     7261 tattattttt tgagacagga tctcaggctg gagtgcagtg gcacaatcac ggctcactgc
     7321 agcctctacc tccccaggct caggtgatcc tcccacctca gcctcccaag tagctgggac
     7381 tataggcaca catcaccaag cccagccaaa ttttgttttt tttttgtaga gatggggttt
     7441 catcacgttg cccaggctga tctcgaacct ctgggctcaa gcaattcact cgcctcggcc
     7501 tcccaaaatg ctgggattac aggcctgagc cactgcgccc agccaggatt tgaattattt
     7561 taactcatcc atgggctgcc ctagaatgtc acaaatgagg gttgtttaat gcctttctta
     7621 tagctgctac tggaacacta ttatgaccta atttatgagc catccttact catctacaag
     7681 tgctgaagca atgttacata cttttttgct aaactcagat tttttagcct aatttcttgt
     7741 cctcctatcc acctgcatcc acacatggcc tgcatggggc tgccttccct gcagtgttct
     7801 gcagccatgc ttcagggtat agctgttggt ggacagcctc aggtcttggg ggcactatag
     7861 ccactaaacg aggtgtgaaa ggctcaagag gatgaccagc aattaattat ccccagaaag
     7921 tgaaggaaaa gagaccttta gggatgttgc tggtcaagtc ttgatttgac cggagtcaaa
     7981 tcaatcttca agcaatcttg gaatcctcaa ctgcagtaag catttcaaaa tgcaaacaaa
     8041 ctgcttaaca actgacaaga caccagccca tacgctgctc ttccaacagt gggttctagc
     8101 tttgaacaaa agtgctaaac atttccttga atatattctt cctctttttg tcctcatcac
     8161 tcaatactgg tgctcttgtc acaggtagaa cagcttgttt cttttccatc tattcaagtg
     8221 tgtttctaat tctaaaatgc tgatcttctc tggagtctat ggtaggcaat tatggtcact
     8281 ggaatagttt gtcttgtttt aaaatattat tggtgcatgt acaacagcat ccaacatatc
     8341 tgtcttgttc ctagatatat agctctgatt ttaggccttt tgtgcatacc attacaatat
     8401 ggtggggtaa gacattctac agtagcctgt gctgaactga tctcttaaat aaacttgctt
     8461 ctggttaact atgttgatga aatctgagag tccattcagt agccctcatc tcacccttca
     8521 aagagagtca gctagcagat tagaaacaaa tgtctgatta ggatgaagca ggtttgaggg
     8581 gtgctggtat aatagcaaga aagatttgct gaccactctc tttccaaact ccatagtttc
     8641 tgagtggaaa cgaggatgag tgaggtcctg gctcaagtat tttactccca ctaccacctg
     8701 ggagccctcc aggaaaagag ctgaggctgc tccagatgga agtcaagagc agctggtatt
     8761 tacacctgga aaatcagatt tttttttctt ttgcaataac tcggtagtaa gaccagacca
     8821 acaattttag agaaaagcct acatctaaca tacatgctac caaacgagtg tctaacagga
     8881 ataaaccaag cagaaatctc aaaacaggtc tacatcaaaa gttacaagca agcatatcag
     8941 gtgcaatact gaggatgagt atattaggaa gtaaaaacac ttaccgtaaa tgaatattcg
     9001 aagtgcacac tgcaatgggt tgattggaga gatatctaaa tgtaaacatt tgacatcaaa
     9061 gtggcaagct ttgggctcca ttggaaggct gcaatttcca tggaaaagcc ctcagagaag
     9121 catgtttcaa ttgccgaaac aggcctaatc cttatgcaag atgtaagggt tttaaaaagt
     9181 tagaaactct gctctcacct ttcaatatta agagcataaa caaatgaatc accaggcaga
     9241 tttttatata tgacactaag agatttagaa ctttaagagc tgtggtgacc acattatggt
     9301 ggaggaacaa aaaaggaaac cttggcagtt agaaagctgc tcagtgatga attcaagaag
     9361 gaaccagctt taaattatct cttcatgcca acatccatct atacactgaa agttttagta
     9421 ttgtacatta agacgatcag gcccagcagg gtggttcacg cctgtaatcc cagaactttg
     9481 ggagactgac atggtggatc acttaagccc aggagtttga gaccagcctg ggcaacatgg
     9541 caaaactgtc tctactaaaa atacaaaaaa actagctggg tgtggtgatg catacctgta
     9601 gtcctaccta cttgagaggc tgaagcagga gaatcgctta aggtcaaggc tgcagtgagc
     9661 tgtgattgtg ccactgcact ccagcctggg tgacacagcg agaccctgtc tctaaaacaa
     9721 aaaagacaat aaagtagttt aagctagtaa aacagaaggt gccacaacaa cctgcaaagc
     9781 cagtgtgaag gaacagcttg aaaaaacttc gaatttctac tgactacaga caaatccaag
     9841 tgctgatatt tttatatcaa ttactggcca aaatggtgtg tttatttttt aaagcttgta
     9901 tcatggaatg tataatctaa tctggaaaaa tgtttgaaag ggatggctag aaaaaaattt
     9961 gggctcacag gcactcacca aataagaacg tcaacaatca ctaaactgca tttttattta
    10021 aacaacatta attacagtc
//
