LOCUS       NM_021117               4131 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens cryptochrome circadian regulator 2 (CRY2), transcript
            variant 1, mRNA.
ACCESSION   NM_021117
VERSION     NM_021117.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Li,F., Guo,L., Zhou,M., Han,L., Wu,S., Wu,L. and Yang,J.
  TITLE     Cryptochrome 2 Suppresses Epithelial-Mesenchymal Transition by
            Promoting Trophoblastic Ferroptosis in Unexplained Recurrent
            Spontaneous Abortion
  JOURNAL   Am J Pathol 194 (7), 1197-1217 (2024)
   PUBMED   38537935
  REMARK    GeneRIF: Cryptochrome 2 Suppresses Epithelial-Mesenchymal
            Transition by Promoting Trophoblastic Ferroptosis in Unexplained
            Recurrent Spontaneous Abortion.
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Parlak,G.C., Baris,I., Gul,S. and Kavakli,I.H.
  TITLE     Functional characterization of the CRY2 circadian clock component
            variant p.Ser420Phe revealed a new degradation pathway for CRY2
  JOURNAL   J Biol Chem 299 (12), 105451 (2023)
   PUBMED   37951306
  REMARK    GeneRIF: Functional characterization of the CRY2 circadian clock
            component variant p.Ser420Phe revealed a new degradation pathway
            for CRY2.
REFERENCE   3  (bases 1 to 4131)
  AUTHORS   Li,W., Xiong,X., Kiperman,T. and Ma,K.
  TITLE     Transcription Repression of CRY2 via PER2 Interaction Promotes
            Adipogenesis
  JOURNAL   Mol Cell Biol 43 (10), 500-514 (2023)
   PUBMED   37724597
  REMARK    GeneRIF: Transcription Repression of CRY2 via PER2 Interaction
            Promotes Adipogenesis.
REFERENCE   4  (bases 1 to 4131)
  AUTHORS   Parlak,G.C., Camur,B.B., Gul,S., Ozcan,O., Baris,I. and
            Kavakli,I.H.
  TITLE     The secondary pocket of cryptochrome 2 is important for the
            regulation of its stability and localization
  JOURNAL   J Biol Chem 298 (9), 102334 (2022)
   PUBMED   35933018
  REMARK    GeneRIF: The secondary pocket of cryptochrome 2 is important for
            the regulation of its stability and localization.
REFERENCE   5  (bases 1 to 4131)
  AUTHORS   Kume,K., Zylka,M.J., Sriram,S., Shearman,L.P., Weaver,D.R., Jin,X.,
            Maywood,E.S., Hastings,M.H. and Reppert,S.M.
  TITLE     mCRY1 and mCRY2 are essential components of the negative limb of
            the circadian clock feedback loop
  JOURNAL   Cell 98 (2), 193-205 (1999)
   PUBMED   10428031
REFERENCE   6  (bases 1 to 4131)
  AUTHORS   van der Horst,G.T., Muijtjens,M., Kobayashi,K., Takano,R.,
            Kanno,S., Takao,M., de Wit,J., Verkerk,A., Eker,A.P., van
            Leenen,D., Buijs,R., Bootsma,D., Hoeijmakers,J.H. and Yasui,A.
  TITLE     Mammalian Cry1 and Cry2 are essential for maintenance of circadian
            rhythms
  JOURNAL   Nature 398 (6728), 627-630 (1999)
   PUBMED   10217146
REFERENCE   7  (bases 1 to 4131)
  AUTHORS   Kobayashi,K., Kanno,S., Smit,B., van der Horst,G.T., Takao,M. and
            Yasui,A.
  TITLE     Characterization of photolyase/blue-light receptor homologs in
            mouse and human cells
  JOURNAL   Nucleic Acids Res 26 (22), 5086-5092 (1998)
   PUBMED   9801304
REFERENCE   8  (bases 1 to 4131)
  AUTHORS   Ishibashi,K., Miyamoto,K., Taketani,Y., Morita,K., Takeda,E.,
            Sasaki,S. and Imai,M.
  TITLE     Molecular cloning of a second human stanniocalcin homologue (STC2)
  JOURNAL   Biochem Biophys Res Commun 250 (2), 252-258 (1998)
   PUBMED   9753616
REFERENCE   9  (bases 1 to 4131)
  AUTHORS   Zhao,S. and Sancar,A.
  TITLE     Human blue-light photoreceptor hCRY2 specifically interacts with
            protein serine/threonine phosphatase 5 and modulates its activity
  JOURNAL   Photochem Photobiol 66 (5), 727-731 (1997)
   PUBMED   9383998
REFERENCE   10 (bases 1 to 4131)
  AUTHORS   Hsu,D.S., Zhao,X., Zhao,S., Kazantsev,A., Wang,R.P., Todo,T.,
            Wei,Y.F. and Sancar,A.
  TITLE     Putative human blue-light photoreceptors hCRY1 and hCRY2 are
            flavoproteins
  JOURNAL   Biochemistry 35 (44), 13871-13877 (1996)
   PUBMED   8909283
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK302865.1, BC041814.1 and
            AC068385.9.
            
            On May 17, 2019 this sequence version replaced NM_021117.4.
            
            Summary: This gene encodes a flavin adenine dinucleotide-binding
            protein that is a key component of the circadian core oscillator
            complex, which regulates the circadian clock. This gene is
            upregulated by CLOCK/ARNTL heterodimers but then represses this
            upregulation in a feedback loop using PER/CRY heterodimers to
            interact with CLOCK/ARNTL. Polymorphisms in this gene have been
            associated with altered sleep patterns. The encoded protein is
            widely conserved across plants and animals. Two transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Feb 2014].
            
            Transcript Variant: This variant (1) uses an alternate first exon
            which contains the predicted translation start site; the resulting
            protein has a distinct N-terminus.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1817366.1,
                                           SRR7346977.1410335.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000616080.2/
                                                  ENSP00000484684.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2199              AK302865.1         70-2268
            2200-2948           BC041814.1         2197-2945
            2949-2949           AC068385.9         56006-56006
            2950-3812           BC041814.1         2947-3809
            3813-3813           AC068385.9         56870-56870
            3814-4131           BC041814.1         3811-4128
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p11.2"
     gene            1..4131
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /note="cryptochrome circadian regulator 2"
                     /db_xref="GeneID:1408"
                     /db_xref="HGNC:HGNC:2385"
                     /db_xref="MIM:603732"
     exon            1..231
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1798
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cryptochrome-2; cryptochrome circadian clock 2;
                     growth-inhibiting protein 37; cryptochrome 2
                     (photolyase-like)"
                     /codon_start=1
                     /product="cryptochrome-2 isoform 1"
                     /protein_id="NP_066940.3"
                     /db_xref="CCDS:CCDS7915.3"
                     /db_xref="GeneID:1408"
                     /db_xref="HGNC:HGNC:2385"
                     /db_xref="MIM:603732"
                     /translation="MAATVATAAAVAPAPAPGTDSASSVHWFRKGLRLHDNPALLAAV
                     RGARCVRCVYILDPWFAASSSVGINRWRFLLQSLEDLDTSLRKLNSRLFVVRGQPADV
                     FPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGVEVVTENSHTLYDLDRIIEL
                     NGQKPPLTYKRFQAIISRMELPKKPVGLVTSQQMESCRAEIQENHDETYGVPSLEELG
                     FPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRF
                     GCLSCRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPIC
                     IQIPWDRNPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVS
                     WESGVRVFDELLLDADFSVNAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRR
                     YLPKLKAFPSRYIYEPWNAPESIQKAAKCIIGVDYPRPIVNHAETSRLNIERMKQIYQ
                     QLSRYRGLCLLASVPSCVEDLSHPVAEPSSSQAGSMSSAGPRPLPSGPASPKRKLEAA
                     EEPPGEELSKRARVAELPTPELPSKDA"
     exon            232..340
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            341..483
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            484..668
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            669..757
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            758..898
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1210
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1362
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1565
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1658
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1800
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..4131
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4109..4114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /note="hexamer: TATAAA"
     polyA_site      4131
                     /gene="CRY2"
                     /gene_synonym="HCRY2; PHLL2"
                     /note="major polyA site"
ORIGIN      
        1 agcagtctgg acagtcatgg cggcgactgt ggcgacggcg gcagctgtgg ccccggcgcc
       61 agcgcccggc acggacagcg cctcttcggt gcactggttc cgcaaagggc tgcgactcca
      121 cgacaacccg gcgttgctgg cggccgtgcg cggggcgcgc tgcgtgcgct gcgtttacat
      181 tctcgacccg tggttcgcgg cctcctcctc agtcgggatc aaccgatgga ggttcctact
      241 tcagtctctg gaagatttgg acacaagttt aaggaaactg aactcccgcc tgtttgtagt
      301 ccggggacag ccagccgacg tgttcccaag gctgttcaag gaatggggag tgacccgctt
      361 gacctttgaa tatgactctg aaccctttgg gaaagaacgg gatgcagcca tcatgaagat
      421 ggccaaggag gctggtgtgg aagtagtgac ggagaattct cataccctct atgacctgga
      481 caggatcatt gagctgaatg ggcagaagcc accccttaca tacaagcgct ttcaggccat
      541 catcagccgc atggagctgc ccaagaagcc agtgggcttg gtgaccagcc agcagatgga
      601 gagctgcagg gccgagatcc aggagaacca cgacgagacc tacggcgtgc cctccctgga
      661 ggagctgggg ttccccactg aaggacttgg tccagctgtc tggcagggag gagagacaga
      721 agctctggcc cgcctggata agcacttgga acggaaggcc tgggttgcca actatgagag
      781 accccgaatg aacgccaact ccctcctggc cagccccaca ggcctcagcc cctacctgcg
      841 ctttggttgt ctctcctgcc gcctcttcta ctaccgcctg tgggacctgt ataaaaaggt
      901 gaagcggaac agcacacctc ccctctccct atttgggcaa ctcctatggc gagagttctt
      961 ctacacggca gctaccaaca accccaggtt tgaccgcatg gaggggaacc ccatctgcat
     1021 ccagatcccc tgggaccgca atcctgaggc cctggccaag tgggctgagg gcaagacagg
     1081 cttcccttgg attgatgcca tcatgaccca actgaggcag gagggctgga tccaccacct
     1141 ggcccggcat gccgtggcct gcttcctgac ccgcggggac ctctgggtca gctgggagag
     1201 cggggtccgg gtatttgatg agctgctcct ggatgcagat ttcagcgtga acgcaggcag
     1261 ctggatgtgg ctgtcctgca gtgctttctt ccagcagttc ttccactgct actgccctgt
     1321 gggctttggc cgtcgcacgg accccagtgg ggactacatc aggcgatacc tgcccaaatt
     1381 gaaagcgttc ccctctcgat acatctatga gccctggaat gccccagagt caattcagaa
     1441 ggcagccaag tgcatcattg gtgtggacta cccacggccc atcgtcaacc atgccgagac
     1501 cagccggctt aacattgaac gaatgaagca gatttaccag cagctttcgc gctaccgggg
     1561 actctgtcta ctggcatctg tcccttcctg tgtggaagac ctcagtcacc ctgtggcaga
     1621 gcccagctcg agccaggctg gcagcatgag cagtgcaggc ccaagaccac tacccagtgg
     1681 cccagcatcc cccaaacgca agctggaagc agccgaggaa ccacctggtg aagaactcag
     1741 caaacgggcc cgggtggcag agttgccaac cccagagctg ccgagcaagg atgcctgaga
     1801 ctgcagagcc cttgctccgt gagcaaagcc tgggtgccca agcagccacc gcagcagcag
     1861 agtacaacct gcagagaagc tgatcaccgg gcagagatag agcgagcatg tgtgtgtgtg
     1921 tgcgcgtgtg cagaggaggg agtggtgtgc ctgtttgtgt gtgcatgcat ctgttgacac
     1981 tcatgattct gaatgttgcc tgggctgggg gagtacctgt agcacgccag tgctgtttcc
     2041 cggcctccag acacaaggct cgaggttatg gcagtgactt tcagctgaga cctgttcctg
     2101 caagccagct gccttgtctg aacagaacgt agtggtagga ccctagctgg gattctggca
     2161 tctgcctccc tagacctcct tccctccctc ctcacgtcag gctgtggagc aggagcacag
     2221 cagttctggc tgttgtccaa agcatgggat tctggaggca gccagagccc tgctgagttc
     2281 ctgctttctg acctggaggc tgagcaggcc ggagtggatg gatgctgtcc agacgtagcc
     2341 acctggcctc tgtttcttat tttaaaattc tctgctactg ggctcagtcc caggcccttc
     2401 cttgggcttc tgggactgag catgaggcca tagacagatc taaaaagttt ccaccaccct
     2461 acagaagtac acacagatac ctgactggtg tggggtatgc ctggtactgt aataggagcc
     2521 taagacagca cacctacctt ttcaggattt agaacctaaa attagaaaga gaatcccagc
     2581 tgtcattgtt ccttccccag aagctaagag ccagcctcag agcctaccca ggagctgtga
     2641 aggggcaagg gtcaaactga ctcactctac caggaggaga ccaggttgca gtggcgtaag
     2701 gccccctggt ttctctggcc acactccaag gcaccacagt gctgccagtg aggacagctg
     2761 acacccagcc agggaaacca ttctagtctt tattctgttg gcttccaggg cctgtcctgg
     2821 acttgtcagc atccagactg ccatgtcagc tatcccagta gctgagctcc aaggactcag
     2881 gcagagggac tcagggatgg ggactgccag gggcagttgg caaaagtcca agtagagatt
     2941 acacccagaa caccattcct tccaggagca gtaggtggga ggtttgaccc agagaagcca
     3001 atccttgcat tccaggagtg gcctgtgcct cccacctctt ccttcccact gccaaaggcc
     3061 tgtgttgaga aagatgtcat gcaaaaggac gacggtggcc aactaaagca agtcttcctt
     3121 ccaccctgtg gcctgcactt gagccacaaa gtgtgtgtgt gtgtgtgcgt gtgtggtacg
     3181 tgtgtgtgtg tgtggctatg aggctgattc ctgtttggat ttttgtcctc acgtgtatca
     3241 ttaagctggc ctttgggcct tttcctttct acctcccctg tgacctttcc tagcctcaga
     3301 tctgttaatt cttttggccc cagccctgtc cctcactgtc ctctgtcctt ggaccagaac
     3361 cctggggtca gacccatctc ctgtagctgt ccatcacact gacaggcttc ttcctgagat
     3421 atcctcaggt tttctcagcc agagagctgc ctttagagtc caactgttgt acgtatgtca
     3481 ccttcactag aaatgtccca tcatcgtggg aggggagcag ggcacagggg atggtgtgca
     3541 ttcagagcat tgggttgggg gcttccctgt tccctcagcc ccagtcgaga ggaaagagaa
     3601 tcgggccact gccagaaaga gagtcaagca aacctggaag ggcaaatctg agagtgggaa
     3661 ggccaaaggc cgaggcccag atttagtatt cactagcagc gccttcgggt agcaggatga
     3721 ttccttttcc tgcctgtctg ctgctggctc tcttccctaa ggtacaggtt ggcaggacca
     3781 cctccgccta cttctccacc atccctagca tgtcagcccg ttcccagatc aacctgccag
     3841 tggagtcagg cagtgcactc ctggagccaa gagggaaggg cagggtagag agggtatgtc
     3901 cagtagcctg gagctccatg gtggcttcat gcctcccttc tcccagctca ggtggccctg
     3961 agggctccct cggaacagtg cctcaaatcc tgacccaagg gccagcatgg ggaagagatg
     4021 gttgcaggca aaatgcactt tatagagatt ttctattgct gggaaggtgt gtttctccca
     4081 caatttgttt gtgaatattc acttgtttta taaatgtctg acctgtcttg a
//
